R. Manjunatha Kini

Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags (2010)

Siang, Ang, Doley, Robin, Vonk, Freek J, Kini, R Manjunatha

Abstract Background The Red-headed krait ( Bungarus flaviceps , Squamata: Serpentes: Elapidae) is a medically important venomous snake that inhabits South-East Asia. Although the venoms of most...

Role of accelerated segment switch in exons to alter targeting (ASSET) in the molecular evolution of snake venom proteins (2009)

Doley, Robin, Mackessy, Stephen P, Kini, R Manjunatha

Abstract Background Snake venom toxins evolve more rapidly than other proteins through accelerated changes in the protein coding regions. Previously we have shown that accelerated segment switch in...

BMC Evolutionary Biology BioMed Central (2009)

Robin Doley, Stephen P Mackessy, R Manjunatha Kini

Research article Role of accelerated segment switch in exons to alter targeting (ASSET) in the molecular evolution of snake venom proteins

Accelerated exchange of exon segments in Viperid three-finger toxin genes (Sistrurus catenatus edwardsii; Desert Massasauga) (2008)

Doley, Robin, Pahari, Susanta, Mackessy, Stephen P, Kini, R Manjunatha

Abstract Background Snake venoms consist primarily of proteins and peptides showing a myriad of potent biological activities which have been shaped by both adaptive and neutral selective forces....

Unusual accelerated rate of deletions and insertions in toxin genes in the venom glands of the pygmy copperhead (Austrelaps labialis) from kangaroo island (2008)

Doley, Robin, Tram, Nguyen, Reza, Md Abu, Kini, R Manjunatha

Abstract Background Toxin profiling helps in cataloguing the toxin present in the venom as well as in searching for novel toxins. The former helps in understanding potential pharmacological profile...

A universal method for fishing target proteins from mixtures of biomolecules using isothermal titration calorimetry (2008)

Zhou, Xingding, Sun, Qingxiang, Kini, R. Manjunatha, Sivaraman, J.

The most challenging tasks in biology include the identification of (1) the orphan receptor for a ligand, (2) the ligand for an orphan receptor protein, and (3) the target protein(s) for a given drug...

The venom gland transcriptome of the Desert Massasauga Rattlesnake (Sistrurus catenatus edwardsii): towards an understanding of venom composition among advanced snakes (Superfamily Colubroidea) (2007)

Pahari, Susanta, Mackessy, Stephen P, Kini, R Manjunatha

Abstract Background Snake venoms are complex mixtures of pharmacologically active proteins and peptides which belong to a small number of superfamilies. Global cataloguing of the venom transcriptome...

Expression pattern of three-finger toxin and phospholipase A2genes in the venom glands of two sea snakes, Lapemis curtusand Acalyptophis peronii: comparison of evolution of these toxins in land snakes, sea kraits and sea snakes (2007)

Pahari, Susanta, Bickford, David, Fry, Bryan G, Kini, R Manjunatha

Abstract Background Snake venom composition varies widely both among closely related species and within the same species, based on ecological variables. In terrestrial snakes, such variation has been...

BMC Molecular Biology 2007, 8:115 doi:10.1186/1471-2199-8-115 (2007)

Susanta Pahari, Stephen P Mackessy, Manjunatha R Kini, Susanta Pahari, Stephen P. Mackessy, R. Manjunatha Kini

PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. The venom gland transcriptome of the Desert Massasauga...

Isolation and characterization of rufoxin, a novel protein exhibiting neurotoxicity from venom of the psammophiine, Rhamphiophis oxyrhynchus (Rufous beaked snake) (2007)

Lumsden, Natalie G., Banerjee, Yajnavalka, Kini, R. Manjunatha, Kuruppu, Sanjaya, Hodgson, Wayne C.

Colubrid snake venoms potentially represent a vast source of novel biological actives and structural motifs owing to their diverse phylogeny. The present study describes the identification of...

Putting the Brakes on Snake Venom Evolution: the Unique Molecular Evolutionary Patterns of Aipysurus eydouxii (Marbled sea snake) Phospholipase A2 Toxins (2005)

Li, Min, Fry, Bryan G., Kini, R. Manjunatha

Accelerated evolution of toxins is a unique feature of venoms, with the toxins evolving via the birth-and-death mode of molecular evolution. The venoms of sea snakes, however, are remarkably simple...

Putting the Brakes on Snake Venom Evolution: The Unique Molecular Evolutionary Patterns of Aipysurus eydouxii (Marbled Sea Snake) Phospholipase A2 Toxins (2005)

Li, Min, Fry, Bryan G., Kini, R. Manjunatha

Accelerated evolution of toxins is a unique feature of venoms, with the toxins evolving via the birth-and-death mode of molecular evolution. The venoms of sea snakes, however, are remarkably simple...

Putting the Brakes on Snake Venom Evolution: the Unique Molecular Evolutionary Patterns of Aipysurus eydouxii (Marbled sea snake) Phospholipase A2 Toxins (2005)

Li, Min, Fry, Bryan G., Kini, R. Manjunatha

Accelerated evolution of toxins is a unique feature of venoms, with the toxins evolving via the birth-and-death mode of molecular evolution. The venoms of sea snakes, however, are remarkably simple...

Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides (2003)

Ajikumar, Parayil Kumaran, Lakshminarayanan, Rajamani, Valiyaveettil, Suresh, Kini, R. Manjunatha

Model peptides were designed, synthesized and conducted a detailed structure-property study to unravel the molecular mechanism of goose eggshell calcification. The peptides were designed based on the...

Eggshell Matrix Protein Mimetics: Elucidation of Molecular Mechanism of Goose Eggshell Calcification using Designed Peptides (2003)

Ajikumar, Parayil Kumaran, Lakshminarayanan, Rajamani, Valiyaveettil, Suresh, Kini, R. Manjunatha

Model peptides were designed, synthesized and conducted a detailed structure-property study to unravel the molecular mechanism of goose eggshell calcification. The peptides were designed based on the...

Designer peptides to understand the mineralization of calcium salts (2003)

Ajikumar, Parayil Kumaran, Lakshminarayanan, Rajamani, Valiyaveettil, Suresh, Kini, R. Manjunatha

Recently, we reported the extraction, purification and amino acid sequence of ansocalcin, the major goose eggshell matrix protein. In vitro studies showed that ansocalcin induces spherical calcite...

Designer peptides to understand the mineralization of calcium salts (2003)

Ajikumar, Parayil Kumaran, Lakshminarayanan, Rajamani, Valiyaveettil, Suresh, Kini, R. Manjunatha

Recently, we reported the extraction, purification and amino acid sequence of ansocalcin, the major goose eggshell matrix protein. In vitro studies showed that ansocalcin induces spherical calcite...

kappa-Hefutoxin1, a novel toxin from the scorpion Heterometrus fulvipes with unique structure and function. Importance of the functional diad in potassium channel selectivity (2002)

Sivaraja, Vaithiyalingam, Sasaki, Toru, Cheng, Betty, Kumar, Thallampuranam Krishnaswamy S, ...

An important and exciting challenge in the postgenomic era is to understand the functions of newly discovered proteins based on their structures. The main thrust is to find the common structural...

Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix

Lakshminarayanan, Rajamani, Kini, R. Manjunatha, Valiyaveettil, Suresh

The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The...

Group D prothrombin activators from snake venom are structural homologues of mammalian blood coagulation factor Xa.

Rao, Veena S, Joseph, Jeremiah S, Kini, R Manjunatha

Procoagulant venoms of several Australian elapids contain proteinases that specifically activate prothrombin; among these, Group D activators are functionally similar to coagulation factor Xa (FXa)....

Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix

Lakshminarayanan, Rajamani, Kini, R. Manjunatha, Valiyaveettil, Suresh

The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The...

Group D prothrombin activators from snake venom are structural homologues of mammalian blood coagulation factor Xa.

Rao, Veena S, Joseph, Jeremiah S, Kini, R Manjunatha

Procoagulant venoms of several Australian elapids contain proteinases that specifically activate prothrombin; among these, Group D activators are functionally similar to coagulation factor Xa (FXa)....

Isolation and pharmacological characterization of a phospholipase A2 myotoxin from the venom of the Irian Jayan death adder (Acanthophis rugosus)

Wickramaratna, Janith C, Fry, Bryan G, Aguilar, Marie-Isabel, Kini, R Manjunatha, Hodgson, Wayne C

It has long been thought that death adder venoms are devoid of myotoxic activity based on studies done on Acanthophis antarcticus (Common death adder) venom. However, a recent clinical study reported...

Biophysical Characterization of Anticoagulant Hemextin AB Complex from the Venom of Snake Hemachatus haemachatus

Banerjee, Yajnavalka, Lakshminarayanan, Rajamani, Vivekanandan, Subramanian, Anand, Ganesh Srinivasan, Valiyaveettil, Suresh, Kini, R. Manjunatha

Hemextin AB complex from the venom of Hemachatus haemachatus is the first known natural anticoagulant that specifically inhibits the enzymatic activity of blood coagulation factor VIIa in the absence...

Crystallization and preliminary X-ray diffraction analysis of hemextin A: a unique anticoagulant protein from Hemachatus haemachatus venom

Banerjee, Yajnavalka, Kumar, Sundramurthy, Jobichen, Chacko, Kini, R. Manjunatha

Crystals of hemextin A, a three-finger toxin isolated and purified from African Ringhals cobra (H. haemachatus), are orthorhombic, space group P212121, with unit-cell parameters a = 49.27, b = 49.51,...

Structural Characterization of Myotoxic Ecarpholin S From Echis carinatus Venom

Zhou, Xingding, Tan, Tien-Chye, Valiyaveettil, S., Go, Mei Lin, Kini, R. Manjunatha, Velazquez-Campoy, Adrian, ...

Phospholipase A2 (PLA2), a common toxic component of snake venom, has been implicated in various pharmacological effects. Ecarpholin S, isolated from the venom of the snake Echis carinatus sochureki,...

A universal method for fishing target proteins from mixtures of biomolecules using isothermal titration calorimetry

Zhou, Xingding, Sun, Qingxiang, Kini, R. Manjunatha, Sivaraman, J.

The most challenging tasks in biology include the identification of (1) the orphan receptor for a ligand, (2) the ligand for an orphan receptor protein, and (3) the target protein(s) for a given drug...