Scientific Commons: R. Meima

Previous work in our group indicated that structural plasmid instability in Bacillus subtilis is often caused by illegitimate recombination between non-repeated sequences, characterized by a...

Essential Bacillus subtilis genes

Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...

To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...

Effects of lysine-to-glycine mutations in the ATP-binding consensus sequences in the AddA and AddB subunits on the Bacillus subtilis AddAB enzyme activities.

Haijema, B J, Meima, R, Kooistra, J, Venema, G

The N-terminal regions of both subunits AddA and AddB of the Bacillus subtilis AddAB enzyme contain amino acid sequences, designated motif I, which are commonly found in ATP-binding enzymes. The...

The expression of a plasmid-specified exported protein causes structural plasmid instability in Bacillus subtilis.

Cordes, C, Meima, R, Twiest, B, Kazemier, B, Venema, G, Van Dijl, J M, ...

The rolling-circle plasmid pGP1 was used to study the effects of the expression of a plasmid-specified exported protein on structural plasmid stability in Bacillus subtilis. pGP1 contains a fusion...

Role of enzymes of homologous recombination in illegitimate plasmid recombination in Bacillus subtilis.

Meima, R, Haijema, B J, Dijkstra, H, Haan, G J, Venema, G, Bron, S

The structural stability of plasmid pGP1, which encodes a fusion between the penicillinase gene (penP) of Bacillus licheniformis and the Escherichia coli lacZ gene, was investigated in Bacillus...

Sequence specificity of illegitimate plasmid recombination in Bacillus subtilis: possible recognition sites for DNA topoisomerase I.

Meima, R, Haan, G J, Venema, G, Bron, S, De Jong, S