The study of the interaction of a model at-helical peptide with lipid bilayers and monolayers (2004)
Vitovic,P., Kresak,S., Naumann,Renate, Schiller,Stefan M., McElhaney,R. N., ...
The study of the interaction of a model at-helical peptide with lipid bilayers and monolayers (2004)
Vitovic, P., Kresak, S., Naumann, Renate, Schiller, Stefan M., McElhaney, R. N., ...
Yamaguchi, S., Takeuchi, K., Mase, T., Oikawa, K., McMullen, T., Derewenda, U., ...
The extracellular lipase from Penicillium camembertii has unique substrate specificity restricted to mono- and diglycerides. The enzyme is a member of a homologous family of lipases from filamentous...
1. 1. Acholeplasma laidlawii B (previously denoted as Mycoplasma laidlawii B) was grown in the presence of equimolar mixtures of palmitic or stearic acid and the following unsaturated fatty acids:...
Mahajan, S, Lewis, R N, George, R, Sykes, B D, McElhaney, R N
The active transport of sodium ions in live Acholeplasma laidlawii B cells and in lipid vesicles containing the (Na+-Mg2+)-ATPase from the plasma membrane of this microorganism was studied by 23Na...
Jinks, D C, Silvius, J R, McElhaney, R N
The membrane-bound adenosine triphosphatase (ATPase) activity of Acholeplasma laidlawii B differs in many respects from the common (Mg2+, Ca2+)-ATPase activity of higher bacteria, most notably in...
The uptake of D-glucose by Acholeplasma laidlawii B occurs via a mediated transport process, as shown by the following observations: (i) glucose permeates A. laidlawii B cells at a rate at least 100...
Influence of Lipid Components of Mycoplasma laidlawii Membranes on Osmotic Fragility of Cells
Razin, S., Tourtellotte, M. E., McElhaney, R. N., Pollack, J. D.
Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells....
Careful analysis of the Arrhenius plot of the Na+,Mg2+-ATPase (ATP pyrophosphohydrolase, EC 3.6.1.8) activity in Acholeplasma laidlawii B membranes of varying fatty acid composition has been combined...
Lewis, R N, Pohle, W, McElhaney, R N
The thermotropic phase behavior of aqueous dispersions of dipalmitoylphosphatidylcholine (DPPC) and its 1,2-dialkyl, 1-acyl 2-alkyl and 1-alkyl 2-acyl analogs was examined by differential scanning...
Lewis, R N, McElhaney, R N, Monck, M A, Cullis, P R
Hydrocarbon chain conformational and orientational order in liquid-crystalline bilayers of the highly chain-asymmetric 1-O-eicosanoyl, 2-O-dodecanoyl and 1-O-decanoyl, 2-O-docosanoyl...
Trouard, T P, Mannock, D A, Lindblom, G, Rilfors, L, Akiyama, M, McElhaney, R N
The hydration properties and the phase structure of 1,2-di-O-tetradecyl-3-O(3-O-methyl-beta-D-glucopyranosyl)-sn-glycerol (3-O-Me-beta-D-GlcDAIG) in water have been studied via differential scanning...
Lewis, R N, McElhaney, R N, Pohle, W, Mantsch, H H
Previous vibrational spectroscopic studies of solid acyl-alkyl and diacyl phosphatidylcholines suggested that the sn1- and sn2-carbonyl stretching modes of 1,2-diacylglycerolipids have different...
The mixed interdigitated gel phases of unlabeled, specifically 13C = O-labeled, and specifically chain-perdeuterated samples of 1-O-eicosanoyl, 2-O-lauroyl phosphatidylcholine and 1-O-decanoyl,...
McMullen, T P, Vilchèze, C, McElhaney, R N, Bittman, R
We have investigated the thermotropic phase behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containing a series of cholesterol analogues varying in the length and structure of their alkyl...
The infrared dichroism of transmembrane helical polypeptides.
Axelsen, P H, Kaufman, B K, McElhaney, R N, Lewis, R N
Polarized attenuated total internal reflectance techniques were applied to study the infrared dichroism of the amide I transition moment in two membrane-bound peptides that are known to form oriented...
The subgel phases of unlabeled, specifically chain perdeuterated and specifically 13C = O labeled representative samples of the n-saturated diacylphosphatidylcholines were studied by...
Mannock, D A, Lewis, R N, McElhaney, R N, Akiyama, M, Yamada, H, Turner, D C, ...
We have studied the physical properties of aqueous dispersions of 1,2-sn- and 2,3-sn-didodecyl-beta-D-glucopyranosyl glycerols, as well as their diastereomeric mixture, using differential scanning...
The polymorphic phase behavior of a homologous series of n-saturated 1,2-diacyl phosphatidylethanolamines was investigated by differential scanning calorimetry, 31P-nuclear magnetic resonance, and...
Lewis, R N, McElhaney, R N, Osterberg, F, Gruner, S M
Twelve saturated mixed-chain phosphatidylcholines have been identified for which the thermotropic phase behavior observed upon cooling from the L alpha phase is dependent upon the thermal history of...
Mannock, D A, McElhaney, R N, Harper, P E, Gruner, S M
We have investigated the thermotropic phase behavior of aqueous dispersions of the 1,2- and 2,3-di-O-tetradecyl-1(3)-O-(beta-D-galactopyranosyl)-sn- glycerols and their diastereomeric mixture using...
McMullen, T P, Lewis, R N, McElhaney, R N
We have investigated the comparative effects of the incorporation of increasing quantities of androstenol and cholesterol on the thermotropic phase behavior of aqueous dispersions of members of a...
Lewis, R N, McElhaney, R N, Harper, P E, Turner, D C, Gruner, S M
We have synthesized a number of 1,2-diacyl phosphatidylcholines with hydrophobic substituents adjacent to the carbonyl group of the fatty acyl chain and studied their thermotropic phase behavior by...
Thermotropic phase behavior of phosphatidylcholines with omega-tertiary-butyl fatty acyl chains.
Lewis, R N, Mantsch, H H, McElhaney, R N
The thermotropic phase behavior of a homologous series of phosphatidylcholines containing acyl chains with omega-tertiary butyl groups was studied by differential scanning calorimetry, Fourier...
Zhang, Y P, Lewis, R N, Hodges, R S, McElhaney, R N
High-sensitivity differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR) spectroscopy were used to study the interaction of a synthetic alpha-helical hydrophobic transmembrane...
The lamellar/nonlamellar phase preferences of lipid model membranes composed of mixtures of several cationic lipids with various zwitterionic and anionic phospholipids were examined by a combination...
The thermotropic phase behavior of lipid bilayer model membranes composed of the even-numbered, N-saturated 1,2-diacyl phosphatidylserines was studied by differential scanning calorimetry and by...
McMullen, T P, Lewis, R N, McElhaney, R N
We have examined the effects of cholesterol on the thermotropic phase behavior and organization of aqueous dispersions of a homologous series of linear disaturated phosphatidylserines by...
Lewis, R N, Winter, I, Kriechbaum, M, Lohner, K, McElhaney, R N
Differential scanning calorimetry, x-ray diffraction, and infrared and (31)P-nuclear magnetic resonance ((31)P-NMR) spectroscopy were used to examine the thermotropic phase behavior and organization...
Harper, P E, Mannock, D A, Lewis, R N, McElhaney, R N, Gruner, S M
X-ray diffraction is used to solve the low-resolution structures of fully hydrated aqueous dispersions of seven different diacyl phosphatidylethanolamines (PEs) whose hydrocarbon chains have the same...
X-ray structure study of thermotropic phases in isoacylphosphatidylcholine multibilayers.
Church, S E, Griffiths, D J, Lewis, R N, McElhaney, R N, Wickman, H H
Structures of lamellar phases in aqueous dispersions of diisoacylphosphatidylcholines (17iPC and 20iPC) were determined by x-ray diffraction methods. In agreement with previous DSC studies, subgel,...
It has recently been proposed that the semipermeable surface barrier of living cells is in fact provided by water polarized in multilayers by the cellular protein, and not by the lipids of the cell...
Mahajan, S, Lewis, R N, George, R, Sykes, B D, McElhaney, R N
The active transport of sodium ions in live Acholeplasma laidlawii B cells and in lipid vesicles containing the (Na+-Mg2+)-ATPase from the plasma membrane of this microorganism was studied by 23Na...
Jinks, D C, Silvius, J R, McElhaney, R N
The membrane-bound adenosine triphosphatase (ATPase) activity of Acholeplasma laidlawii B differs in many respects from the common (Mg2+, Ca2+)-ATPase activity of higher bacteria, most notably in...
The uptake of D-glucose by Acholeplasma laidlawii B occurs via a mediated transport process, as shown by the following observations: (i) glucose permeates A. laidlawii B cells at a rate at least 100...
Influence of Lipid Components of Mycoplasma laidlawii Membranes on Osmotic Fragility of Cells
Razin, S., Tourtellotte, M. E., McElhaney, R. N., Pollack, J. D.
Razin, S. (University of Connecticut, Storrs), M. E. Tourtellotte, R. N. McElhaney, and J. D. Pollack. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells....
Careful analysis of the Arrhenius plot of the Na+,Mg2+-ATPase (ATP pyrophosphohydrolase, EC 3.6.1.8) activity in Acholeplasma laidlawii B membranes of varying fatty acid composition has been combined...
Lewis, R N, Pohle, W, McElhaney, R N
The thermotropic phase behavior of aqueous dispersions of dipalmitoylphosphatidylcholine (DPPC) and its 1,2-dialkyl, 1-acyl 2-alkyl and 1-alkyl 2-acyl analogs was examined by differential scanning...
Lewis, R N, McElhaney, R N, Monck, M A, Cullis, P R
Hydrocarbon chain conformational and orientational order in liquid-crystalline bilayers of the highly chain-asymmetric 1-O-eicosanoyl, 2-O-dodecanoyl and 1-O-decanoyl, 2-O-docosanoyl...
Trouard, T P, Mannock, D A, Lindblom, G, Rilfors, L, Akiyama, M, McElhaney, R N
The hydration properties and the phase structure of 1,2-di-O-tetradecyl-3-O(3-O-methyl-beta-D-glucopyranosyl)-sn-glycerol (3-O-Me-beta-D-GlcDAIG) in water have been studied via differential scanning...
Lewis, R N, McElhaney, R N, Pohle, W, Mantsch, H H
Previous vibrational spectroscopic studies of solid acyl-alkyl and diacyl phosphatidylcholines suggested that the sn1- and sn2-carbonyl stretching modes of 1,2-diacylglycerolipids have different...
The mixed interdigitated gel phases of unlabeled, specifically 13C = O-labeled, and specifically chain-perdeuterated samples of 1-O-eicosanoyl, 2-O-lauroyl phosphatidylcholine and 1-O-decanoyl,...
McMullen, T P, Vilchèze, C, McElhaney, R N, Bittman, R
We have investigated the thermotropic phase behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containing a series of cholesterol analogues varying in the length and structure of their alkyl...
The infrared dichroism of transmembrane helical polypeptides.
Axelsen, P H, Kaufman, B K, McElhaney, R N, Lewis, R N
Polarized attenuated total internal reflectance techniques were applied to study the infrared dichroism of the amide I transition moment in two membrane-bound peptides that are known to form oriented...
The subgel phases of unlabeled, specifically chain perdeuterated and specifically 13C = O labeled representative samples of the n-saturated diacylphosphatidylcholines were studied by...
Mannock, D A, Lewis, R N, McElhaney, R N, Akiyama, M, Yamada, H, Turner, D C, ...
We have studied the physical properties of aqueous dispersions of 1,2-sn- and 2,3-sn-didodecyl-beta-D-glucopyranosyl glycerols, as well as their diastereomeric mixture, using differential scanning...
The polymorphic phase behavior of a homologous series of n-saturated 1,2-diacyl phosphatidylethanolamines was investigated by differential scanning calorimetry, 31P-nuclear magnetic resonance, and...
Lewis, R N, McElhaney, R N, Osterberg, F, Gruner, S M
Twelve saturated mixed-chain phosphatidylcholines have been identified for which the thermotropic phase behavior observed upon cooling from the L alpha phase is dependent upon the thermal history of...
Mannock, D A, McElhaney, R N, Harper, P E, Gruner, S M
We have investigated the thermotropic phase behavior of aqueous dispersions of the 1,2- and 2,3-di-O-tetradecyl-1(3)-O-(beta-D-galactopyranosyl)-sn- glycerols and their diastereomeric mixture using...
McMullen, T P, Lewis, R N, McElhaney, R N
We have investigated the comparative effects of the incorporation of increasing quantities of androstenol and cholesterol on the thermotropic phase behavior of aqueous dispersions of members of a...
Lewis, R N, McElhaney, R N, Harper, P E, Turner, D C, Gruner, S M
We have synthesized a number of 1,2-diacyl phosphatidylcholines with hydrophobic substituents adjacent to the carbonyl group of the fatty acyl chain and studied their thermotropic phase behavior by...
Thermotropic phase behavior of phosphatidylcholines with omega-tertiary-butyl fatty acyl chains.
Lewis, R N, Mantsch, H H, McElhaney, R N
The thermotropic phase behavior of a homologous series of phosphatidylcholines containing acyl chains with omega-tertiary butyl groups was studied by differential scanning calorimetry, Fourier...
Zhang, Y P, Lewis, R N, Hodges, R S, McElhaney, R N
High-sensitivity differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR) spectroscopy were used to study the interaction of a synthetic alpha-helical hydrophobic transmembrane...
The lamellar/nonlamellar phase preferences of lipid model membranes composed of mixtures of several cationic lipids with various zwitterionic and anionic phospholipids were examined by a combination...
The thermotropic phase behavior of lipid bilayer model membranes composed of the even-numbered, N-saturated 1,2-diacyl phosphatidylserines was studied by differential scanning calorimetry and by...
McMullen, T P, Lewis, R N, McElhaney, R N
We have examined the effects of cholesterol on the thermotropic phase behavior and organization of aqueous dispersions of a homologous series of linear disaturated phosphatidylserines by...
Lewis, R N, Winter, I, Kriechbaum, M, Lohner, K, McElhaney, R N
Differential scanning calorimetry, x-ray diffraction, and infrared and (31)P-nuclear magnetic resonance ((31)P-NMR) spectroscopy were used to examine the thermotropic phase behavior and organization...
Harper, P E, Mannock, D A, Lewis, R N, McElhaney, R N, Gruner, S M
X-ray diffraction is used to solve the low-resolution structures of fully hydrated aqueous dispersions of seven different diacyl phosphatidylethanolamines (PEs) whose hydrocarbon chains have the same...
X-ray structure study of thermotropic phases in isoacylphosphatidylcholine multibilayers.
Church, S E, Griffiths, D J, Lewis, R N, McElhaney, R N, Wickman, H H
Structures of lamellar phases in aqueous dispersions of diisoacylphosphatidylcholines (17iPC and 20iPC) were determined by x-ray diffraction methods. In agreement with previous DSC studies, subgel,...
It has recently been proposed that the semipermeable surface barrier of living cells is in fact provided by water polarized in multilayers by the cellular protein, and not by the lipids of the cell...
McDonough, B., Macdonald, P. M., Sykes, B. D., McElhaney, R. N.
The technique of 19F-nuclear magnetic resonance (19F-NMR) spectroscopy offers a number of advantages for studies of lipid fatty acyl chain orientation and dynamics in biomembranes. However, the...