INTELLIGIBILITY OF THE PHONETIC ALPHABET WHEN MASKED BY RANDOM WHITE NOISE, (2005)
The Listen Study was designed to develop and test a training method to increase the ability of Air Force personnel in identifying verbal signals masked by jamming. Findings relating to alphabet...
Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene.
A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS...
Saporito, S M, Gedenk, M, Cunningham, R P
The role of exonuclease III and endonuclease IV in the repair of pyrimidine dimers in bacteriophage T4-infected Escherichia coli was examined. UV-irradiated T4 showed reduced survival when plated on...
Nucleotide sequence of the xth gene of Escherichia coli K-12.
Saporito, S M, Smith-White, B J, Cunningham, R P
The xth gene of Escherichia coli K-12, which encodes exonuclease III, has been sequenced. Exonuclease III from a cloned copy of the E. coli K-12 gene has been purified and characterized. The...
Nucleotide sequence of the nfo gene of Escherichia coli K-12.
Saporito, S M, Cunningham, R P
The nfo gene of Escherichia coli K-12 which encodes endonuclease IV has been sequenced. The predicted gene product has a molecular weight of 31,562, in good agreement with the size of the gene...
Endonuclease IV (nfo) mutant of Escherichia coli.
Cunningham, R P, Saporito, S M, Spitzer, S G, Weiss, B
A cloned gene, designated nfo, caused overproduction of an EDTA-resistant endonuclease specific for apurinic-apyrimidinic sites in DNA. The sedimentation coefficient of the enzyme was similar to that...
Escherichia coli K-12 mutants in which viability is dependent on recA function.
A gene required for growth and viability in recA mutants of Escherichia coli K-12 was identified. This gene, rdgB (for Rec-dependent growth), mapped near 64 min on the E. coli genetic map. In a...
The nth gene of Escherichia coli affects the production of endonuclease III, a glycosylase-endonuclease that attacks DNA damaged by oxidizing agents or by ionizing radiation. An nth insertion mutant...
Folded chromosomes were isolated from Eschericia coli thermosensitive dnaA initiation mutants incubated at the nonpermissive temperature and were analyzed by neutral sucrose density gradient...
IST2: an insertion sequence from Thiobacillus ferrooxidans.
Yates, J R, Cunningham, R P, Holmes, D S
The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined....
T4 endonuclease VII cleaves the crossover strands of Holliday junction analogs.
Mueller, J E, Kemper, B, Cunningham, R P, Kallenbach, N R, Seeman, N C
We have formed four-arm branched DNA junctions that contain no more than a single base pair of branch migratory freedom. Recently, we have shown that these Holliday junction analogs have twofold...
Polarity of heteroduplex formation promoted by Escherichia coli recA protein.
Kahn, R, Cunningham, R P, DasGupta, C, Radding, C M
When recA protein pairs circular single strands with linear duplex DNA, the circular strand displaces its homolog from only one end of the duplex molecule and rapidly creates heteroduplex joints that...
Enzymatic recognition of DNA modifications induced by singlet oxygen and photosensitizers.
Müller, E, Boiteux, S, Cunningham, R P, Epe, B
DNA modifications induced either by photosensitization (illumination in the presence of methylene blue) or by chemically generated singlet oxygen (thermal decomposition of an...
Shibata, T, DasGupta, C, Cunningham, R P, Radding, C M
Escherichia coli single-strand binding protein (SSB) or phage T4 gene 32 protein reduced the amount of recA protein required to catalyze the formation of D loops from double-stranded DNA and...
Doetsch, P W, Henner, W D, Cunningham, R P, Toney, J H, Helland, D E
We have compared the sites of nucleotide incision on DNA damaged by oxidizing agents when cleavage is mediated by either Escherichia coli endonuclease III or an endonuclease present in bovine and...
Shibata, T, DasGupta, C, Cunningham, R P, Radding, C M
Purified Escherichia coli recA protein catalyzed ATP-dependent pairing of superhelical DNA and homologous single-stranded fragments. The product of the reaction: (i) was retained by nitrocellulose...
Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure.
Thayer, M M, Ahern, H, Xing, D, Cunningham, R P, Tainer, J A
The 1.85 A crystal structure of endonuclease III, combined with mutational analysis, suggests the structural basis for the DNA binding and catalytic activity of the enzyme. Helix-hairpin-helix (HhH)...
Endonuclease III (nth) mutants of Escherichia coli.
Two strains that overproduce endonuclease III were found in a colony bank containing hybrid ColE1-Escherichia coli plasmids. The enzyme was identified in crude extracts by the degradation of...
Homologous pairing in genetic recombination: complexes of recA protein and DNA.
Shibata, T, Cunningham, R P, DasGupta, C, Radding, C M
recA protein, which is essential for general genetic recombination in Escherichia coli, promotes the homologous pairing of single-stranded DNA with double-stranded DNA to form a D loop. The amount of...
Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene.
A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS...
Saporito, S M, Gedenk, M, Cunningham, R P
The role of exonuclease III and endonuclease IV in the repair of pyrimidine dimers in bacteriophage T4-infected Escherichia coli was examined. UV-irradiated T4 showed reduced survival when plated on...
Nucleotide sequence of the xth gene of Escherichia coli K-12.
Saporito, S M, Smith-White, B J, Cunningham, R P
The xth gene of Escherichia coli K-12, which encodes exonuclease III, has been sequenced. Exonuclease III from a cloned copy of the E. coli K-12 gene has been purified and characterized. The...
Nucleotide sequence of the nfo gene of Escherichia coli K-12.
Saporito, S M, Cunningham, R P
The nfo gene of Escherichia coli K-12 which encodes endonuclease IV has been sequenced. The predicted gene product has a molecular weight of 31,562, in good agreement with the size of the gene...
Endonuclease IV (nfo) mutant of Escherichia coli.
Cunningham, R P, Saporito, S M, Spitzer, S G, Weiss, B
A cloned gene, designated nfo, caused overproduction of an EDTA-resistant endonuclease specific for apurinic-apyrimidinic sites in DNA. The sedimentation coefficient of the enzyme was similar to that...
Escherichia coli K-12 mutants in which viability is dependent on recA function.
A gene required for growth and viability in recA mutants of Escherichia coli K-12 was identified. This gene, rdgB (for Rec-dependent growth), mapped near 64 min on the E. coli genetic map. In a...
The nth gene of Escherichia coli affects the production of endonuclease III, a glycosylase-endonuclease that attacks DNA damaged by oxidizing agents or by ionizing radiation. An nth insertion mutant...
Folded chromosomes were isolated from Eschericia coli thermosensitive dnaA initiation mutants incubated at the nonpermissive temperature and were analyzed by neutral sucrose density gradient...
IST2: an insertion sequence from Thiobacillus ferrooxidans.
Yates, J R, Cunningham, R P, Holmes, D S
The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined....
T4 endonuclease VII cleaves the crossover strands of Holliday junction analogs.
Mueller, J E, Kemper, B, Cunningham, R P, Kallenbach, N R, Seeman, N C
We have formed four-arm branched DNA junctions that contain no more than a single base pair of branch migratory freedom. Recently, we have shown that these Holliday junction analogs have twofold...
Polarity of heteroduplex formation promoted by Escherichia coli recA protein.
Kahn, R, Cunningham, R P, DasGupta, C, Radding, C M
When recA protein pairs circular single strands with linear duplex DNA, the circular strand displaces its homolog from only one end of the duplex molecule and rapidly creates heteroduplex joints that...
Enzymatic recognition of DNA modifications induced by singlet oxygen and photosensitizers.
Müller, E, Boiteux, S, Cunningham, R P, Epe, B
DNA modifications induced either by photosensitization (illumination in the presence of methylene blue) or by chemically generated singlet oxygen (thermal decomposition of an...
Shibata, T, DasGupta, C, Cunningham, R P, Radding, C M
Escherichia coli single-strand binding protein (SSB) or phage T4 gene 32 protein reduced the amount of recA protein required to catalyze the formation of D loops from double-stranded DNA and...
Doetsch, P W, Henner, W D, Cunningham, R P, Toney, J H, Helland, D E
We have compared the sites of nucleotide incision on DNA damaged by oxidizing agents when cleavage is mediated by either Escherichia coli endonuclease III or an endonuclease present in bovine and...
Shibata, T, DasGupta, C, Cunningham, R P, Radding, C M
Purified Escherichia coli recA protein catalyzed ATP-dependent pairing of superhelical DNA and homologous single-stranded fragments. The product of the reaction: (i) was retained by nitrocellulose...
Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure.
Thayer, M M, Ahern, H, Xing, D, Cunningham, R P, Tainer, J A
The 1.85 A crystal structure of endonuclease III, combined with mutational analysis, suggests the structural basis for the DNA binding and catalytic activity of the enzyme. Helix-hairpin-helix (HhH)...
Endonuclease III (nth) mutants of Escherichia coli.
Two strains that overproduce endonuclease III were found in a colony bank containing hybrid ColE1-Escherichia coli plasmids. The enzyme was identified in crude extracts by the degradation of...
Homologous pairing in genetic recombination: complexes of recA protein and DNA.
Shibata, T, Cunningham, R P, DasGupta, C, Radding, C M
recA protein, which is essential for general genetic recombination in Escherichia coli, promotes the homologous pairing of single-stranded DNA with double-stranded DNA to form a D loop. The amount of...