R. P. Siraganian

Tyrosine phosphorylation coupled to IgE receptor-mediated signal transduction and histamine release.

Benhamou, M, Gutkind, J S, Robbins, K C, Siraganian, R P

Antigen-induced cross-linking of IgE bound to its receptors at the surface of basophils or mast cells initiates a number of biochemical events culminating in the release of histamine-containing...

Conformational changes induced in the protein tyrosine kinase p72syk by tyrosine phosphorylation or by binding of phosphorylated immunoreceptor tyrosine-based activation motif peptides.

Kimura, T, Sakamoto, H, Appella, E, Siraganian, R P

A critical event in signaling in immune cells is the interaction of Syk or ZAP-70 protein tyrosine kinases with multisubunit receptors that contain an approximately 18-amino-acid domain called the...

Characterization of monoclonal antibodies to fimbria-associated adhesins of Bacteroides loescheii PK1295.

Weiss, E I, London, J, Kolenbrander, P E, Andersen, R N, Fischler, C, Siraganian, R P

Bacteroides loescheii PK1295 fimbriae, which mediate the lactose-sensitive coaggregation with Streptococcus sanguis 34 and the lactose-insensitive coaggregation with Actinomyces israelii PK14, were...

Human and rat mast cell high-affinity immunoglobulin E receptors: characterization of putative alpha-chain gene products.

Shimizu, A, Tepler, I, Benfey, P N, Berenstein, E H, Siraganian, R P, Leder, P

We have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative alpha subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs...

Rat basophilic leukemia cell lines defective in phospholipid methyltransferase enzymes, Ca2+ influx, and histamine release: reconstitution by hybridization.

McGivney, A, Crews, F T, Hirata, F, Axelrod, J, Siraganian, R P

Variants of the rat basophilic leukemia (RBL) cell line were isolated and screened for phospholipid methyltransferase I and II activities, enzymes that convert phosphatidylethanolamine to...

A unique insert in the linker domain of Syk is necessary for its function in immunoreceptor signalling.

Latour, S, Zhang, J, Siraganian, R P, Veillette, A

Accumulating data indicate that the 'linker' region of Syk, which lies between its tandem Src homology 2 (SH2) domains and kinase region, provides a critical function for the biological activity of...

Tyrosine phosphorylation coupled to IgE receptor-mediated signal transduction and histamine release.

Benhamou, M, Gutkind, J S, Robbins, K C, Siraganian, R P

Antigen-induced cross-linking of IgE bound to its receptors at the surface of basophils or mast cells initiates a number of biochemical events culminating in the release of histamine-containing...

Conformational changes induced in the protein tyrosine kinase p72syk by tyrosine phosphorylation or by binding of phosphorylated immunoreceptor tyrosine-based activation motif peptides.

Kimura, T, Sakamoto, H, Appella, E, Siraganian, R P

A critical event in signaling in immune cells is the interaction of Syk or ZAP-70 protein tyrosine kinases with multisubunit receptors that contain an approximately 18-amino-acid domain called the...

Characterization of monoclonal antibodies to fimbria-associated adhesins of Bacteroides loescheii PK1295.

Weiss, E I, London, J, Kolenbrander, P E, Andersen, R N, Fischler, C, Siraganian, R P

Bacteroides loescheii PK1295 fimbriae, which mediate the lactose-sensitive coaggregation with Streptococcus sanguis 34 and the lactose-insensitive coaggregation with Actinomyces israelii PK14, were...

Human and rat mast cell high-affinity immunoglobulin E receptors: characterization of putative alpha-chain gene products.

Shimizu, A, Tepler, I, Benfey, P N, Berenstein, E H, Siraganian, R P, Leder, P

We have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative alpha subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs...

Rat basophilic leukemia cell lines defective in phospholipid methyltransferase enzymes, Ca2+ influx, and histamine release: reconstitution by hybridization.

McGivney, A, Crews, F T, Hirata, F, Axelrod, J, Siraganian, R P

Variants of the rat basophilic leukemia (RBL) cell line were isolated and screened for phospholipid methyltransferase I and II activities, enzymes that convert phosphatidylethanolamine to...

A unique insert in the linker domain of Syk is necessary for its function in immunoreceptor signalling.

Latour, S, Zhang, J, Siraganian, R P, Veillette, A

Accumulating data indicate that the 'linker' region of Syk, which lies between its tandem Src homology 2 (SH2) domains and kinase region, provides a critical function for the biological activity of...

Rapid phosphorylation of a 92,000 MW protein on activation of rat basophilic leukaemia cells for histamine release.

Hattori, Y, Siraganian, R P

Many cellular functions are modulated by phosphorylation of proteins. The IgE- or ionophore-mediated activation of rat basophilic leukaemia cells (RBL-2H3) was accompanied by the prominent...

IgE antibody suppression following aerosol exposure to antigens.

Fox, P C, Siraganian, R P

Exposure of mice to aerosolized antigens induced a low level IgG1 response but not detectable IgE antibodies. Subsequent intraperitoneal immunization of these mice demonstrated immunoglobulin...

Influence of anions, cations and osmolarity on IgE-mediated histamine release from human basophils.

Hook, W A, Siraganian, R P

Allergen-activated human basophils require Ca2+ for the in vitro release of histamine. This study evaluated the importance of anions, cations and osmotic pressure on histamine release from human...

Enhancement of IgE-mediated histamine release from human basophils by immune-specific lymphokines.

Ida, S, Hooks, J J, Siraganian, R P, Notkins, A L

Human leucocytes (basophils) release histamine when exposed to ragweed antigen E or anti-IgE. The present study shows that when leucocytes from BCG-positive donors are first incubated with PPD and...

The mechanism of basophil histamine release in patients with periodontal disease.

Olsson-Wennström, A, Wennström, J L, Mergenhagen, S E, Siraganian, R P

Histamine release from washed peripheral blood basophils of thirty-three subjects with varying degrees of periodontal disease was studied. Dental plaque, serum and basophil leucocytes were collected...