Radical changes to chlamydial taxonomy are not necessary just yet (letter and reply) (2001)
Schachter, J., Stephens, R.S., Timms, P., Kuo, C., Bavoil, P.M., Birkelund, S., ...
Cytotoxic-T-lymphocyte-mediated cytolysis of L cells persistently infected with Chlamydia spp.
Rasmussen, S J, Timms, P, Beatty, P R, Stephens, R S
Persistent chlamydial infections have been proposed as a means whereby chlamydiae evade immune resolution of infection. Such a mechanism would require evasion not only of the humoral immune responses...
Beatty, P R, Rasmussen, S J, Stephens, R S
Cells infected with Chlamydia trachomatis are lysed by CD8+ T cells in vitro. The ability of C. trachomatis-elicited spleen cells to lyse target cells infected with other chlamydial strains was...
We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC[GGCCGC), SgrAI (C(A/G)[CCGG(T/G)G), and Sse8387I (CCTGCA[GG), and we analyzed...
The 60,000-molecular-weight cysteine-rich outer membrane protein (OMP2) from Chlamydia trachomatis participates in the disulfide-mediated outer-membrane organization unique to this organism. In...
Stephens, R S, Wagar, E A, Edman, U
Chlamydia trachomatis has a biphasic developmental cycle which is characterized by qualitative and quantitative changes in protein expression. The molecular mechanisms that mediate these changes are...
Sequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2.
Stephens, R S, Mullenbach, G, Sanchez-Pescador, R, Agabian, N
The structural gene for the major outer membrane protein (MOMP) from Chlamydia trachomatis was cloned and sequenced. A lambda gt11 recombinant (lambda gt11/L2/33) that contains a portion of the MOMP...
Diversity of Chlamydia trachomatis major outer membrane protein genes.
Stephens, R S, Sanchez-Pescador, R, Wagar, E A, Inouye, C, Urdea, M S
Genomic DNA libraries were constructed for Chlamydia trachomatis serovars B and C by using BamHI fragments, and recombinants that contained the major outer membrane protein (omp1) gene for each...
Ishizaki, M, Allen, J E, Beatty, P R, Stephens, R S
The antigenically variant Chlamydia trachomatis major outer membrane protein (MOMP) is a target of antibody-mediated neutralization in vitro, and it is an important protein for designing a subunit...
Cerrone, M C, Ma, J J, Stephens, R S
We isolated and sequenced the gene for the chlamydial heat shock protein 60 (HSP-60) from a Chlamydia trachomatis genomic library by molecular genetic methods. The DNA sequence derived revealed an...
Dean, D, Patton, M, Stephens, R S
The nucleotide sequences of variable segments (VS) 1, 2, and 4 for the major outer membrane protein gene (omp1) of Chlamydia trachomatis were determined for serologically defined subtypes D', I', and...
Identification of Chlamydia trachomatis antigens by use of murine T-cell lines.
Chlamydia-specific short-term T-cell lines were used in conjunction with immunoblot techniques to examine Chlamydia trachomatis proteins for T-cell-stimulatory activity. This study was undertaken...
Developmental-form-specific DNA-binding proteins in Chlamydia spp.
We identified DNA-binding proteins specific to the elementary body (EB) developmental form of Chlamydia spp. Chlamydial proteins from whole lysates were separated by polyacrylamide gel...
Stephens, R S, Kuo, C C, Newport, G, Agabian, N
DNA obtained from Chlamydia trachomatis (serovar L2) was partially digested with DNase I and inserted into the beta-galactosidase gene of bacteriophage lambda gt11. Seven recombinants were selected...
Tubulin and high-molecular-weight polypeptides as Giardia lamblia antigens.
Torian, B E, Barnes, R C, Stephens, R S, Stibbs, H H
We determined the antigenic specificities of nine murine monoclonal antibodies to Giardia lamblia. Four distinct antibody reaction patterns were detected by immunofluorescence with G. lamblia...
Chlamydia trachomatis species-specific epitope detected on mouse biovar outer membrane protein.
A common antigenic determinant on the chlamydial major outer membrane protein was detected on each of the three Chlamydia trachomatis biovars (trachoma, lymphogranuloma venereum, and mouse). This...
Specific and common antigens of Trichomonas vaginalis detected by monoclonal antibodies.
Torian, B E, Connelly, R J, Stephens, R S, Stibbs, H H
Monoclonal antibodies to Trichomonas vaginalis were prepared by immunizing mice with a cloned isolate of T. vaginalis. Eight antibodies reacted with the same four isolates or strains but did not...
Genetic polymorphisms in the major outer membrane protein gene (omp-1) of Chlamydia trachomatis B and Ba serovars have been demonstrated in Tunisian isolates. A total of 15 of 27 unique sequence...
Stephens, R S, Kuo, C C, Tam, M R
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell...
Zhao, Q, Schachter, J, Stephens, R S
The major outer membrane protein gene (omp1) was sequenced for each of six Chlamydia psittaci (guinea pig inclusion conjunctivitis [GPIC]) strains isolated from guinea pigs. Five of the isolates were...
Stephens, R S, Chen, W J, Kuo, C C
Suppression of the inflammatory reaction with daily doses of cortisone acetate or cyclophosphamide substantially prolonged the pulmonary infection in mice which had been intranasally inoculated with...
Rasmussen, S J, Eckmann, L, Quayle, A J, Shen, L, Zhang, Y X, Anderson, D J, ...
Chlamydia species infect epithelial cells at mucosal surfaces, and are major causes of sexually transmitted diseases. Infection is characterized by inflammation which is exacerbated upon reinfection,...
Cytotoxic-T-lymphocyte-mediated cytolysis of L cells persistently infected with Chlamydia spp.
Rasmussen, S J, Timms, P, Beatty, P R, Stephens, R S
Persistent chlamydial infections have been proposed as a means whereby chlamydiae evade immune resolution of infection. Such a mechanism would require evasion not only of the humoral immune responses...
Beatty, P R, Rasmussen, S J, Stephens, R S
Cells infected with Chlamydia trachomatis are lysed by CD8+ T cells in vitro. The ability of C. trachomatis-elicited spleen cells to lyse target cells infected with other chlamydial strains was...
We constructed the physical map of Chlamydia trachomatis serovar L2 by using three restriction endonucleases, NotI (GC[GGCCGC), SgrAI (C(A/G)[CCGG(T/G)G), and Sse8387I (CCTGCA[GG), and we analyzed...
The 60,000-molecular-weight cysteine-rich outer membrane protein (OMP2) from Chlamydia trachomatis participates in the disulfide-mediated outer-membrane organization unique to this organism. In...
Stephens, R S, Wagar, E A, Edman, U
Chlamydia trachomatis has a biphasic developmental cycle which is characterized by qualitative and quantitative changes in protein expression. The molecular mechanisms that mediate these changes are...
Sequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2.
Stephens, R S, Mullenbach, G, Sanchez-Pescador, R, Agabian, N
The structural gene for the major outer membrane protein (MOMP) from Chlamydia trachomatis was cloned and sequenced. A lambda gt11 recombinant (lambda gt11/L2/33) that contains a portion of the MOMP...
Diversity of Chlamydia trachomatis major outer membrane protein genes.
Stephens, R S, Sanchez-Pescador, R, Wagar, E A, Inouye, C, Urdea, M S
Genomic DNA libraries were constructed for Chlamydia trachomatis serovars B and C by using BamHI fragments, and recombinants that contained the major outer membrane protein (omp1) gene for each...
Ishizaki, M, Allen, J E, Beatty, P R, Stephens, R S
The antigenically variant Chlamydia trachomatis major outer membrane protein (MOMP) is a target of antibody-mediated neutralization in vitro, and it is an important protein for designing a subunit...
Cerrone, M C, Ma, J J, Stephens, R S
We isolated and sequenced the gene for the chlamydial heat shock protein 60 (HSP-60) from a Chlamydia trachomatis genomic library by molecular genetic methods. The DNA sequence derived revealed an...
Dean, D, Patton, M, Stephens, R S
The nucleotide sequences of variable segments (VS) 1, 2, and 4 for the major outer membrane protein gene (omp1) of Chlamydia trachomatis were determined for serologically defined subtypes D', I', and...
Identification of Chlamydia trachomatis antigens by use of murine T-cell lines.
Chlamydia-specific short-term T-cell lines were used in conjunction with immunoblot techniques to examine Chlamydia trachomatis proteins for T-cell-stimulatory activity. This study was undertaken...
Developmental-form-specific DNA-binding proteins in Chlamydia spp.
We identified DNA-binding proteins specific to the elementary body (EB) developmental form of Chlamydia spp. Chlamydial proteins from whole lysates were separated by polyacrylamide gel...
Stephens, R S, Kuo, C C, Newport, G, Agabian, N
DNA obtained from Chlamydia trachomatis (serovar L2) was partially digested with DNase I and inserted into the beta-galactosidase gene of bacteriophage lambda gt11. Seven recombinants were selected...
Tubulin and high-molecular-weight polypeptides as Giardia lamblia antigens.
Torian, B E, Barnes, R C, Stephens, R S, Stibbs, H H
We determined the antigenic specificities of nine murine monoclonal antibodies to Giardia lamblia. Four distinct antibody reaction patterns were detected by immunofluorescence with G. lamblia...
Chlamydia trachomatis species-specific epitope detected on mouse biovar outer membrane protein.
A common antigenic determinant on the chlamydial major outer membrane protein was detected on each of the three Chlamydia trachomatis biovars (trachoma, lymphogranuloma venereum, and mouse). This...
Specific and common antigens of Trichomonas vaginalis detected by monoclonal antibodies.
Torian, B E, Connelly, R J, Stephens, R S, Stibbs, H H
Monoclonal antibodies to Trichomonas vaginalis were prepared by immunizing mice with a cloned isolate of T. vaginalis. Eight antibodies reacted with the same four isolates or strains but did not...
Genetic polymorphisms in the major outer membrane protein gene (omp-1) of Chlamydia trachomatis B and Ba serovars have been demonstrated in Tunisian isolates. A total of 15 of 27 unique sequence...
Stephens, R S, Kuo, C C, Tam, M R
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell...
Zhao, Q, Schachter, J, Stephens, R S
The major outer membrane protein gene (omp1) was sequenced for each of six Chlamydia psittaci (guinea pig inclusion conjunctivitis [GPIC]) strains isolated from guinea pigs. Five of the isolates were...
Stephens, R S, Chen, W J, Kuo, C C
Suppression of the inflammatory reaction with daily doses of cortisone acetate or cyclophosphamide substantially prolonged the pulmonary infection in mice which had been intranasally inoculated with...
Rasmussen, S J, Eckmann, L, Quayle, A J, Shen, L, Zhang, Y X, Anderson, D J, ...
Chlamydia species infect epithelial cells at mucosal surfaces, and are major causes of sexually transmitted diseases. Infection is characterized by inflammation which is exacerbated upon reinfection,...
Davies, A F, Imaizumi, K, Mirza, G, Stephens, R S, Kuroki, Y, Matsuno, M, ...
Chromosomal translocations affecting the 6p24 region have been associated with orofacial clefting. Here we present a female patient with cleft palate, severe growth retardation, developmental delay,...