Scientific Commons: R. W. Buckheit

The phosphorothioate oligonucleotide T2G4T2 was identified as an inhibitor of HIV infection in vitro by combinatorial screening of a library of phosphorothioate oligonucleotides that contained all...

3'-Azido-3'-deoxythymidine-induced reduction in the ability of uninfected CD4-expressing cells to participate in syncytium formation.

Buckheit, R W, Germany-Decker, J, Qualls-Goodwin, K, Bowdon, B J, Shannon, W M

A cocultivation assay system consisting of uninfected human T cells and cells chronically infected with human immunodeficiency virus type 1 has been used to investigate syncytium formation in...

Michellamine B, a novel plant alkaloid, inhibits human immunodeficiency virus-induced cell killing by at least two distinct mechanisms.

McMahon, J B, Currens, M J, Gulakowski, R J, Buckheit, R W, Lackman-Smith, C, Hallock, Y F, ...

Studies of the mechanism of action of michellamine B, a novel anti-human immunodeficiency virus (HIV) alkaloid from the tropical plant Ancistrocladus korupensis, have revealed that the compound acts...

T30177, an oligonucleotide stabilized by an intramolecular guanosine octet, is a potent inhibitor of laboratory strains and clinical isolates of human immunodeficiency virus type 1.

Ojwang, J O, Buckheit, R W, Pommier, Y, Mazumder, A, De Vreese, K, Esté, J A, ...

T30177, an oligonucleotide composed of only deoxyguanosine and thymidine, is 17 nucleotides in length and contains single phosphorothioate internucleoside linkages at its 5' and 3' ends for...

Structure-activity and cross-resistance evaluations of a series of human immunodeficiency virus type-1-specific compounds related to oxathiin carboxanilide.

Buckheit, R W, Kinjerski, T L, Fliakas-Boltz, V, Russell, J D, Stup, T L, Pallansch, L A, ...

A series of compounds related to the nonnucleoside reverse transcriptase (RT) inhibitor (NNRTI) oxathiin carboxanilide (UC84) were evaluated for activity against the human immunodeficiency virus...

Inhibition of multiple phases of human immunodeficiency virus type 1 replication by a dithiane compound that attacks the conserved zinc fingers of retroviral nucleocapsid proteins.

Rice, W G, Baker, D C, Schaeffer, C A, Graham, L, Bu, M, Terpening, S, ...

The human immunodeficiency virus type 1 (HIV-1) nucleocapsid p7 protein contains two retrovirus-type zinc finger domains that are required for multiple phases of viral replication. Chelating residues...

Highly potent oxathiin carboxanilide derivatives with efficacy against nonnucleoside reverse transcriptase inhibitor-resistant human immunodeficiency virus isolates.

Buckheit, R W, Snow, M J, Fliakas-Boltz, V, Kinjerski, T L, Russell, J D, Pallansch, L A, ...

The structure-activity relationships of a series of compounds related to the nonnucleoside reverse transcriptase (RT) inhibitor (NNRTI) oxathiin carboxanilide have been described (R. W. Buckheit,...

Discovery of cyanovirin-N, a novel human immunodeficiency virus-inactivating protein that binds viral surface envelope glycoprotein gp120: potential applications to microbicide development.

Boyd, M R, Gustafson, K R, McMahon, J B, Shoemaker, R H, O'Keefe, B R, Mori, T, ...

We have isolated and sequenced a novel 11-kDa virucidal protein, named cyanovirin-N (CV-N), from cultures of the cyanobacterium (blue-green alga) Nostoc ellipsosporum. We also have produced CV-N...

Diarylsulfones, a new chemical class of nonnucleoside antiviral inhibitors of human immunodeficiency virus type 1 reverse transcriptase.

McMahon, J B, Gulakowski, R J, Weislow, O S, Schultz, R J, Narayanan, V L, Clanton, D J, ...

A series of variously substituted diarylsulfones and related derivatives were found to prevent human immunodeficiency virus type 1 (HIV-1) replication and HIV-1-induced cell killing in vitro. One of...

Inhibition of HIV-LTR gene expression by oligonucleotides targeted to the TAR element.

Vickers, T, Baker, B F, Cook, P D, Zounes, M, Buckheit, R W, Germany, J, ...

All human immunodeficiency virus mRNAs contain a sequence known as TAR (trans-activating responsive sequence). The TAR element forms a stable RNA stem-loop structure which binds the HIV tat...

Decontamination of an HIV-contaminated CPR manikin.

Corless, I B, Lisker, A, Buckheit, R W

There has been a concern that the number of persons engaging in cardiopulmonary resuscitation (CPR) training could decline because of questions about human immunodeficiency virus (HIV-1)...

Combinatorially selected guanosine-quartet structure is a potent inhibitor of human immunodeficiency virus envelope-mediated cell fusion.

Wyatt, J R, Vickers, T A, Roberson, J L, Buckheit, R W, Klimkait, T, DeBaets, E, ...