A baboon model for hematologic studies of cardiopulmonary bypass (1997)
Hiramatsu, Y., Gikakis, N., Gorman, J.H., Khan, M.M.H., Hack, C.E., Velthuis, H. Te, ...
Pixley, R A, Colman, R W, Schapira, M
To define the factors responsible for the inactivation of the active fragment derived from Factor XII (Factor XIIf ) in plasma, we studied the inactivation kinetics of Factor XIIf in various purified...
The intrinsic pathway of blood coagulation is activated when factor XIa, one of the three contact-system enzymes, is generated and then activates factor IX. Factor XI has been shown to be efficiently...
Grant, P G, Mannarino, A F, Colman, R W
Treatment of intact human platelets with the adenylate cyclase agonist forskolin (100 microM) resulted in an increase in cAMP phosphodiesterase activity in freeze-thaw lysates. When the low-Km (high...
Platelet hypersensitivity induced by cholesterol incorporation.
Shattil, S J, Anaya-Galindo, R, Bennett, J, Colman, R W, Cooper, R A
Platelets from individuals with familial hypercholesterolemia show increased sensitivity to the aggregating atents, epinephrine and ADP. Since the mechanism of this abnormal sensitivity is unknown,...
Wong, P Y, Talamo, R C, Williams, G H, Colman, R W
The possibility that bradykinin, a potent vasodilator, might be a physiological antagonist of the renin-angiotensin system was investigated. 11 norman subjects, ranging in age from 21 to 33 yr were...
Human neutrophils contain and bind high molecular weight kininogen.
Gustafson, E J, Schmaier, A H, Wachtfogel, Y T, Kaufman, N, Kucich, U, Colman, R W
Because plasma kallikrein activates human neutrophils, and in plasma prekallikrein (PK) circulates complexed with high molecular weight kininogen (HMWK), we determined whether HMWK could mediate...
Subcellular localization and secretion of factor V from human platelets.
Chesney, C M, Pifer, D, Colman, R W
Factor V, a plasma protein cofactor necessary for optimal conversion of prothrombin to thrombin, is also present in considerable concentration in blood platelets (9.9 units per 10(9) platelets)....
Pixley, R A, Page, J D, Kaufman, N, Wyshock, E G, Chang, A, ...
The hypotension and disseminated intravascular coagulation (DIC) in bacteremia is thought to be mediated by the combined actions of cytokines, prostaglandins, and complement. The contact system, via...
Colman, R W, Bagdasarian, A, Talamo, R C, Scott, C F, Seavey, M, Guimaraes, J A, ...
An asymptomatic woman (Ms. Williams) was found to have a severe abnormality in the surface-activated intrinsic coagulation, fibrinolytic, and kinin-generating pathways. Assays for known coagulation...
Prostaglandin E1 (PGE1) covalently linked to omega-NH2-hexyl-agarose (PGE1-hexyl-agarose) stimulates the activity of human platelet adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1]...
Purified human plasma kallikrein aggregates human blood neutrophils.
Schapira, M, Despland, E, Scott, C F, Boxer, L A, Colman, R W
Exposure of human blood polymorphonuclear leukocytes (PMN) to purified active plasma kallikrein resulted in PMN aggregation when kallikrein was present at concentrations ranging from 0.4 to 0.6 U/ml...
Human plasma kallikrein releases neutrophil elastase during blood coagulation.
Wachtfogel, Y T, Kucich, U, James, H L, Scott, C F, Schapira, M, Zimmerman, M, ...
Elastase is released from human neutrophils during the early events of blood coagulation. Human plasma kallikrein has been shown to stimulate neutrophil chemotaxis, aggregation, and oxygen...
Isolation of alpha 1-protease inhibitor from human normal and malignant ovarian tissue.
Bagdasarian, A, Wheeler, J, Stewart, G J, Ahmed, S S, Colman, R W
Proteolytic enzymes are associated with normal and neoplastic tissues. Therefore protease inhibitors might also be involved in the control of cell function. alpha 1-protease antigen and antitryptic...
Potentiation of the function of Hageman factor fragments by high molecular weight kininogen.
Liu, C Y, Scott, C F, Bagdasarian, A, Pierce, J V, Kaplan, A P, Colman, R W
Patients lacking high molecular weight (HMW) kininogen have profound abnormalities of the Hageman factor-dependent pathways of coagulation, kinin formation, and fibrinolysis. The ability of HMW...
Meier, H L, Pierce, J V, Colman, R W, Kaplan, A P
The activation and function of surface-bound Hageman factor in human plasma are dependent upon both high molecular weight (HMW) kininogen and prekallikrein. HMW kininogen does not affect the binding...
De Agostini, A, Schapira, M, Wachtfogel, Y T, Colman, R W, Carrel, S
The inactivation of human plasma kallikrein (EC 3.4.21.8) by the inhibitor of activated complement component 1 (C1 inhibitor) induces the formation of a 1:1 stoichiometric kallikrein-C1 inhibitor...
Scott, C F, Carrell, R W, Glaser, C B, Kueppers, F, Lewis, J H, Colman, R W
Alpha-1-antitrypsin-Pittsburgh is a human variant that resulted from a point mutation in the plasma protease inhibitor, alpha 1-antitrypsin (358 Met----Arg). This defect in the alpha 1-antitrypsin...
Platelet C1- inhibitor. A secreted alpha-granule protein.
Schmaier, A H, Smith, P M, Colman, R W
In order to characterize which proteins of the contact phase of coagulation interact with platelets, human platelets were studied immunochemically and functionally to determine if they contain C1-...
Biosynthesis of factor V in isolated guinea pig megakaryocytes.
Chiu, H C, Schick, P K, Colman, R W
Although platelets contain Factor V, localized primarily in the alpha-granules, the origin of this coagulation cofactor in these cells is not known. We therefore explored whether isolated...
A unique elastase in human blood platelets.
James, H L, Wachtfogel, Y T, James, P L, Zimmerman, M, Colman, R W, Cohen, A B
Previous investigations suggested that elastolytic activity found in platelets could be due to contamination by neutrophil elastase. In the present study, the lysate of blood platelets free of...
High molecular weight kininogen is an inhibitor of platelet calpain.
Schmaier, A H, Bradford, H, Silver, L D, Farber, A, Scott, C F, Schutsky, D, ...
Recent studies from our laboratory indicate that a high concentration of platelet-derived calcium-activated cysteine protease (calpain) can cleave high molecular weight kininogen (HMWK). On...
Scott, C F, Silver, L D, Schapira, M, Colman, R W
High molecular weight kininogen (HMW)-kininogen, the cofactor of contact-activated blood coagulation, accelerates the activation of Factor XII, prekallikrein, and Factor XI on a negatively charged...
Identification of prekallikrein and high-molecular-weight kininogen as a complex in human plasma.
Mandle, R J, Colman, R W, Kaplan, A P
Prekallikrein and high-molecular-weight kininogen were found associated in normal human plasma at a molecular weight of 285,000 as assessed by gel filtration on Sephadex G-200. The molecular weight...
High-molecular weight kininogen. A secreted platelet protein.
Schmaier, A H, Zuckerberg, A, Silverman, C, Kuchibhotla, J, Tuszynski, G P, Colman, R W
Human platelets were studied immunochemically to determine if they contain high-molecular weight kininogen. On crossed immunoelectrophoresis with total kininogen antisera (antisera that recognizes...
Inactivation of factor XII active fragment in normal plasma. Predominant role of C-1-inhibitor.
De Agostini, A, Lijnen, H R, Pixley, R A, Colman, R W, Schapira, M
To define the factors responsible for the inactivation of the active fragment derived from Factor XII (Factor XIIf ) in plasma, we studied the inactivation kinetics of Factor XIIf in various purified...
Wachtfogel, Y T, Abrams, W, Kucich, U, Weinbaum, G, Schapira, M, Colman, R W
We investigated whether adhesive glycoproteins, such as fibronectin or fibrinogen, could function to provide a nidus for neutrophil degranulation. Elastase release in recalcified plasma was normal in...
Chien, P, Pixley, R A, Stumpo, L G, Colman, R W, Schreiber, A D
Macrophage Fc gamma receptors play a significant role in inflammation and host defense. One monocyte/macrophage Fc gamma receptor, Fc gamma RI, the binding site for monomeric IgG, appears to be...
Colman, R W, Pixley, R A, Najamunnisa, S, Yan, W, Wang, J, Mazar, A, ...
The urokinase receptor (uPAR) binds urokinase-type plasminogen activator (u-PA) through specific interactions with uPAR domain 1, and vitronectin through interactions with a site within uPAR domains...
Inhibition of thromboxane A2 synthesis in human platelets by coagulation factor Xa.
Sinha, A K, Rao, A K, Willis, J, Colman, R W
Factor Xa binds to platelets provided that factor Va is present on the platelet surface, an interaction that results in a striking acceleration of the conversion of prothrombin to thrombin. Thrombin...
Kinetics of inhibition of platelet calpain II by human kininogens.
Bradford, H N, Schmaier, A H, Colman, R W
The plasma kininogens, high-molecular-mass and low-molecular-mass kininogens, are the most potent plasma inhibitors of platelet calpain. We explored the kinetic mechanisms for kininogen inhibition of...
A simple assay for adenylate cyclase in intact cells by affinity elution chromatography.
A method using the principle of affinity elution chromatography is described for the assay of adenylate cyclase in intact human platelets. By incubating platelet-rich plasma in the presence of...
Ghazaleh, F A, Omburo, G A, Colman, R W
Camp is a major regulator of platelet function. cGMP-inhibited phosphodiesterase (cGI-PDE) is the predominant platelet enzyme hydrolysing cAMP. The pH-rate profile plot for this enzyme yields pKa...
The intrinsic pathway of blood coagulation is activated when factor XIa, one of the three contact-system enzymes, is generated and then activates factor IX. Factor XI has been shown to be efficiently...
Grant, P G, Mannarino, A F, Colman, R W
Treatment of intact human platelets with the adenylate cyclase agonist forskolin (100 microM) resulted in an increase in cAMP phosphodiesterase activity in freeze-thaw lysates. When the low-Km (high...
Platelet hypersensitivity induced by cholesterol incorporation.
Shattil, S J, Anaya-Galindo, R, Bennett, J, Colman, R W, Cooper, R A
Platelets from individuals with familial hypercholesterolemia show increased sensitivity to the aggregating atents, epinephrine and ADP. Since the mechanism of this abnormal sensitivity is unknown,...
Wong, P Y, Talamo, R C, Williams, G H, Colman, R W
The possibility that bradykinin, a potent vasodilator, might be a physiological antagonist of the renin-angiotensin system was investigated. 11 norman subjects, ranging in age from 21 to 33 yr were...
Human neutrophils contain and bind high molecular weight kininogen.
Gustafson, E J, Schmaier, A H, Wachtfogel, Y T, Kaufman, N, Kucich, U, Colman, R W
Because plasma kallikrein activates human neutrophils, and in plasma prekallikrein (PK) circulates complexed with high molecular weight kininogen (HMWK), we determined whether HMWK could mediate...
Subcellular localization and secretion of factor V from human platelets.
Chesney, C M, Pifer, D, Colman, R W
Factor V, a plasma protein cofactor necessary for optimal conversion of prothrombin to thrombin, is also present in considerable concentration in blood platelets (9.9 units per 10(9) platelets)....
Pixley, R A, Page, J D, Kaufman, N, Wyshock, E G, Chang, A, ...
The hypotension and disseminated intravascular coagulation (DIC) in bacteremia is thought to be mediated by the combined actions of cytokines, prostaglandins, and complement. The contact system, via...
Colman, R W, Bagdasarian, A, Talamo, R C, Scott, C F, Seavey, M, Guimaraes, J A, ...
An asymptomatic woman (Ms. Williams) was found to have a severe abnormality in the surface-activated intrinsic coagulation, fibrinolytic, and kinin-generating pathways. Assays for known coagulation...
Prostaglandin E1 (PGE1) covalently linked to omega-NH2-hexyl-agarose (PGE1-hexyl-agarose) stimulates the activity of human platelet adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1]...
Purified human plasma kallikrein aggregates human blood neutrophils.
Schapira, M, Despland, E, Scott, C F, Boxer, L A, Colman, R W
Exposure of human blood polymorphonuclear leukocytes (PMN) to purified active plasma kallikrein resulted in PMN aggregation when kallikrein was present at concentrations ranging from 0.4 to 0.6 U/ml...
Human plasma kallikrein releases neutrophil elastase during blood coagulation.
Wachtfogel, Y T, Kucich, U, James, H L, Scott, C F, Schapira, M, Zimmerman, M, ...
Elastase is released from human neutrophils during the early events of blood coagulation. Human plasma kallikrein has been shown to stimulate neutrophil chemotaxis, aggregation, and oxygen...
Isolation of alpha 1-protease inhibitor from human normal and malignant ovarian tissue.
Bagdasarian, A, Wheeler, J, Stewart, G J, Ahmed, S S, Colman, R W
Proteolytic enzymes are associated with normal and neoplastic tissues. Therefore protease inhibitors might also be involved in the control of cell function. alpha 1-protease antigen and antitryptic...
Potentiation of the function of Hageman factor fragments by high molecular weight kininogen.
Liu, C Y, Scott, C F, Bagdasarian, A, Pierce, J V, Kaplan, A P, Colman, R W
Patients lacking high molecular weight (HMW) kininogen have profound abnormalities of the Hageman factor-dependent pathways of coagulation, kinin formation, and fibrinolysis. The ability of HMW...
Meier, H L, Pierce, J V, Colman, R W, Kaplan, A P
The activation and function of surface-bound Hageman factor in human plasma are dependent upon both high molecular weight (HMW) kininogen and prekallikrein. HMW kininogen does not affect the binding...
De Agostini, A, Schapira, M, Wachtfogel, Y T, Colman, R W, Carrel, S
The inactivation of human plasma kallikrein (EC 3.4.21.8) by the inhibitor of activated complement component 1 (C1 inhibitor) induces the formation of a 1:1 stoichiometric kallikrein-C1 inhibitor...
Scott, C F, Carrell, R W, Glaser, C B, Kueppers, F, Lewis, J H, Colman, R W
Alpha-1-antitrypsin-Pittsburgh is a human variant that resulted from a point mutation in the plasma protease inhibitor, alpha 1-antitrypsin (358 Met----Arg). This defect in the alpha 1-antitrypsin...
Platelet C1- inhibitor. A secreted alpha-granule protein.
Schmaier, A H, Smith, P M, Colman, R W
In order to characterize which proteins of the contact phase of coagulation interact with platelets, human platelets were studied immunochemically and functionally to determine if they contain C1-...
Biosynthesis of factor V in isolated guinea pig megakaryocytes.
Chiu, H C, Schick, P K, Colman, R W
Although platelets contain Factor V, localized primarily in the alpha-granules, the origin of this coagulation cofactor in these cells is not known. We therefore explored whether isolated...
A unique elastase in human blood platelets.
James, H L, Wachtfogel, Y T, James, P L, Zimmerman, M, Colman, R W, Cohen, A B
Previous investigations suggested that elastolytic activity found in platelets could be due to contamination by neutrophil elastase. In the present study, the lysate of blood platelets free of...
High molecular weight kininogen is an inhibitor of platelet calpain.
Schmaier, A H, Bradford, H, Silver, L D, Farber, A, Scott, C F, Schutsky, D, ...
Recent studies from our laboratory indicate that a high concentration of platelet-derived calcium-activated cysteine protease (calpain) can cleave high molecular weight kininogen (HMWK). On...
Scott, C F, Silver, L D, Schapira, M, Colman, R W
High molecular weight kininogen (HMW)-kininogen, the cofactor of contact-activated blood coagulation, accelerates the activation of Factor XII, prekallikrein, and Factor XI on a negatively charged...
Identification of prekallikrein and high-molecular-weight kininogen as a complex in human plasma.
Mandle, R J, Colman, R W, Kaplan, A P
Prekallikrein and high-molecular-weight kininogen were found associated in normal human plasma at a molecular weight of 285,000 as assessed by gel filtration on Sephadex G-200. The molecular weight...
High-molecular weight kininogen. A secreted platelet protein.
Schmaier, A H, Zuckerberg, A, Silverman, C, Kuchibhotla, J, Tuszynski, G P, Colman, R W
Human platelets were studied immunochemically to determine if they contain high-molecular weight kininogen. On crossed immunoelectrophoresis with total kininogen antisera (antisera that recognizes...
Inactivation of factor XII active fragment in normal plasma. Predominant role of C-1-inhibitor.
De Agostini, A, Lijnen, H R, Pixley, R A, Colman, R W, Schapira, M
To define the factors responsible for the inactivation of the active fragment derived from Factor XII (Factor XIIf ) in plasma, we studied the inactivation kinetics of Factor XIIf in various purified...
Wachtfogel, Y T, Abrams, W, Kucich, U, Weinbaum, G, Schapira, M, Colman, R W
We investigated whether adhesive glycoproteins, such as fibronectin or fibrinogen, could function to provide a nidus for neutrophil degranulation. Elastase release in recalcified plasma was normal in...
Chien, P, Pixley, R A, Stumpo, L G, Colman, R W, Schreiber, A D
Macrophage Fc gamma receptors play a significant role in inflammation and host defense. One monocyte/macrophage Fc gamma receptor, Fc gamma RI, the binding site for monomeric IgG, appears to be...
Colman, R W, Pixley, R A, Najamunnisa, S, Yan, W, Wang, J, Mazar, A, ...
The urokinase receptor (uPAR) binds urokinase-type plasminogen activator (u-PA) through specific interactions with uPAR domain 1, and vitronectin through interactions with a site within uPAR domains...
Inhibition of thromboxane A2 synthesis in human platelets by coagulation factor Xa.
Sinha, A K, Rao, A K, Willis, J, Colman, R W
Factor Xa binds to platelets provided that factor Va is present on the platelet surface, an interaction that results in a striking acceleration of the conversion of prothrombin to thrombin. Thrombin...
Kinetics of inhibition of platelet calpain II by human kininogens.
Bradford, H N, Schmaier, A H, Colman, R W
The plasma kininogens, high-molecular-mass and low-molecular-mass kininogens, are the most potent plasma inhibitors of platelet calpain. We explored the kinetic mechanisms for kininogen inhibition of...
A simple assay for adenylate cyclase in intact cells by affinity elution chromatography.
A method using the principle of affinity elution chromatography is described for the assay of adenylate cyclase in intact human platelets. By incubating platelet-rich plasma in the presence of...
Ghazaleh, F A, Omburo, G A, Colman, R W
Camp is a major regulator of platelet function. cGMP-inhibited phosphodiesterase (cGI-PDE) is the predominant platelet enzyme hydrolysing cAMP. The pH-rate profile plot for this enzyme yields pKa...
Activation of the contact system in lethal hypotensive bacteremia in a baboon model.
Pixley, R. A., DeLa Cadena, R. A., Page, J. D., Kaufman, N., Wyshock, E. G., Colman, R. W., ...
The hypotension in septicemia is believed to be mediated by the combined action of many mediators including cytokines, prostaglandins, and complement components. To evaluate the contribution of the...
A primate model of hyperacute renal allograft rejection.
Busch, G. J., Martins, A. C., Hollenberg, N. K., Wilson, R. E., Colman, R. W.
Hyperacute renal allograft rejection is initiated by primary immune injury to vascular endothelium and is propagated by secondary vasoconstriction, platelet aggregation and intravascular coagulation....
Busch, G. J., Kobayashi, K., Hollenberg, N. K., Birtch, A. G., Colman, R. W.
Two groups of specifically presensitized Macaca speciosa monkeys received renal allografts via anastomosis to an indwelling arteriovenous (A-V) shunt. One group was pretreated with heparin (2 mg/kg)...
Busch, G. J., Martins, A. C., Hollenberg, N. K., Moretz, R. C., Wilson, R. E., Colman, R. W.
Inhibition of renal vasoconstriction during hyperacute rejection by phenoxybenzamine or methylprednisolone combined with either the antiplatelet agent pyridinolcarbamate or heparin was evaluted in...
Colman, R. W., Habal, M., Hollenberg, N. K., Birtch, A. G., Busch, G. J.
Two antiplatelet drugs, pyridinolcarbamate and Sudoxicam, were tested separately and in combination with heparin for their ability to modify experimental hyperacute renal allograft rejection in...
DeLa Cadena, R. A., Colman, R. W.
The histidine-glycine-rich region of the light chain of cleaved high molecular weight kininogen (HK) is thought to be responsible for binding to negatively charged surfaces and initiation of the...