R. Y. Wang

Publication List Details

Period

2008 - 2008

Number

28

Co-Authors

Material parameter measurements for ferroelectrics using the partial unloading method. (2008)

Zhou, D., Wang, R.Y., Kamlah, M., Laskewitz, B., Gan, Y.

SPIE Smart Structures : Smart Structures and Materials and Nondestructive Evaluation and Health Monitoring ; 15th Annual Internat.Symp., San Diego, Calif., March 9-13, 2008

DNA methylation inhibits the transfecting activity of replicative- form phi X174 DNA.

Wang, R Y, Shenoy, S, Ehrlich, M

Replacement of virtually all the cytosine residues with 5-methylcytosine residues in the complementary strand of the replicative form (RF) of phi X174 DNA caused a 300- to 500-fold loss in its...

5-methyl-dCTP deaminase induced by bacteriophage XP-12.

Wang, R Y, Ehrlich, M

Bacteriophage XP-12, whose DNA contains 34 mol% 5-methylcytosine, induces the synthesis of a unique enzyme, 5-methyl-dCTP deaminase. The substrate for this enzyme, 5-methyl-dCTP, is produced by...

Selective detection of Mycoplasma fermentans by polymerase chain reaction and by using a nucleotide sequence within the insertion sequence-like element.

Wang, R Y, Hu, W S, Dawson, M S, Shih, J W, Lo, S C

A new assay using the polymerase chain reaction to amplify a 206-nucleotide specific gene sequence within the insertion sequence-like element of Mycoplasma fermentans has been developed. The unique...

Effect of site-specific DNA methylation and mutagenesis on recognition by methylated DNA-binding protein from human placenta.

Zhang, X Y, Ehrlich, K C, Wang, R Y, Ehrlich, M

Methylated DNA-binding protein (MDBP) from human placenta is the first protein shown to bind specifically to certain DNA sequences only when they are methylated at cytosine residues. Among the sites...

Human placental DNA methyltransferase: DNA substrate and DNA binding specificity.

Wang, R Y, Huang, L H, Ehrlich, M

We have partially purified a DNA methyltransferase from human placenta using a novel substrate for a highly sensitive assay of methylation of hemimethylated DNA. This substrate was prepared by...

Human DNA sequences exhibiting gamete-specific hypomethylation.

Zhang, X Y, Wang, R Y, Ehrlich, M

Three human DNA sequences have been cloned from DNA regions which are strikingly undermethylated in sperm, highly methylated in adult somatic tissues, and methylated to an intermediate extent in...

Comparison of bisulfite modification of 5-methyldeoxycytidine and deoxycytidine residues.

Wang, R Y, Gehrke, C W, Ehrlich, M

Sodium bisulfite is a mutagen which can specifically deaminate more than 96% of the cytosine residues in single-stranded DNA via formation of a 5,6-dihydrocytosine-6-sulfonate intermediate. Under the...

The 5-methylcytosine content of highly repeated sequences in human DNA.

Gama-Sosa, M A, Wang, R Y, Kuo, K C, Gehrke, C W, Ehrlich, M

Previously, we found much tissue- or cell-specificity in the levels of 5-methylcytosine (m5C) in the total human genome as well as in DNA fractions resolved by reassociation kinetics. We now report...

A human DNA-binding protein is methylation-specific and sequence-specific.

Wang, R Y, Zhang, X Y, Ehrlich, M

A nuclear protein isolated from human placenta, methylated DNA-binding protein (MDBP), binds selectively to DNA enriched in 5-methylcytosine. We now demonstrate that MDBP is a sequence-specific, as...

Methylated DNA-binding protein from human placenta recognizes specific methylated sites on several prokaryotic DNAs.

Wang, R Y, Zhang, X Y, Khan, R, Zhou, Y W, Huang, L H, Ehrlich, M

Methylated DNA-binding protein (MDBP) from human placenta recognizes specific DNA sequences containing 5-methylcytosine (m5C) residues. Comparisons of binding of various prokaryotic DNAs to MDBP...

DNA methylation inhibits the transfecting activity of replicative- form phi X174 DNA.

Wang, R Y, Shenoy, S, Ehrlich, M

Replacement of virtually all the cytosine residues with 5-methylcytosine residues in the complementary strand of the replicative form (RF) of phi X174 DNA caused a 300- to 500-fold loss in its...

5-methyl-dCTP deaminase induced by bacteriophage XP-12.

Wang, R Y, Ehrlich, M

Bacteriophage XP-12, whose DNA contains 34 mol% 5-methylcytosine, induces the synthesis of a unique enzyme, 5-methyl-dCTP deaminase. The substrate for this enzyme, 5-methyl-dCTP, is produced by...

Selective detection of Mycoplasma fermentans by polymerase chain reaction and by using a nucleotide sequence within the insertion sequence-like element.

Wang, R Y, Hu, W S, Dawson, M S, Shih, J W, Lo, S C

A new assay using the polymerase chain reaction to amplify a 206-nucleotide specific gene sequence within the insertion sequence-like element of Mycoplasma fermentans has been developed. The unique...

Effect of site-specific DNA methylation and mutagenesis on recognition by methylated DNA-binding protein from human placenta.

Zhang, X Y, Ehrlich, K C, Wang, R Y, Ehrlich, M

Methylated DNA-binding protein (MDBP) from human placenta is the first protein shown to bind specifically to certain DNA sequences only when they are methylated at cytosine residues. Among the sites...

Human placental DNA methyltransferase: DNA substrate and DNA binding specificity.

Wang, R Y, Huang, L H, Ehrlich, M

We have partially purified a DNA methyltransferase from human placenta using a novel substrate for a highly sensitive assay of methylation of hemimethylated DNA. This substrate was prepared by...

Human DNA sequences exhibiting gamete-specific hypomethylation.

Zhang, X Y, Wang, R Y, Ehrlich, M

Three human DNA sequences have been cloned from DNA regions which are strikingly undermethylated in sperm, highly methylated in adult somatic tissues, and methylated to an intermediate extent in...

Comparison of bisulfite modification of 5-methyldeoxycytidine and deoxycytidine residues.

Wang, R Y, Gehrke, C W, Ehrlich, M

Sodium bisulfite is a mutagen which can specifically deaminate more than 96% of the cytosine residues in single-stranded DNA via formation of a 5,6-dihydrocytosine-6-sulfonate intermediate. Under the...

The 5-methylcytosine content of highly repeated sequences in human DNA.

Gama-Sosa, M A, Wang, R Y, Kuo, K C, Gehrke, C W, Ehrlich, M

Previously, we found much tissue- or cell-specificity in the levels of 5-methylcytosine (m5C) in the total human genome as well as in DNA fractions resolved by reassociation kinetics. We now report...

A human DNA-binding protein is methylation-specific and sequence-specific.

Wang, R Y, Zhang, X Y, Ehrlich, M

A nuclear protein isolated from human placenta, methylated DNA-binding protein (MDBP), binds selectively to DNA enriched in 5-methylcytosine. We now demonstrate that MDBP is a sequence-specific, as...

Methylated DNA-binding protein from human placenta recognizes specific methylated sites on several prokaryotic DNAs.

Wang, R Y, Zhang, X Y, Khan, R, Zhou, Y W, Huang, L H, Ehrlich, M

Methylated DNA-binding protein (MDBP) from human placenta recognizes specific DNA sequences containing 5-methylcytosine (m5C) residues. Comparisons of binding of various prokaryotic DNAs to MDBP...

Unruptured right coronary sinus to left ventricle aneurysm diagnosed by cross sectional echocardiography.

Mok, C K, Cheung, K L, Wang, R Y

A 37 year old Chinese man presenting with features of chest infection was diagnosed as having severe aortic regurgitation. Cross sectional echocardiography showed an intact right coronary sinus of...

Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy

Zhang, T., Wang, R.Y., Bao, Q-Y., Rawson, D.M.

Information on fish embryo membrane permeability is vital in their cryopreservation. Whilst conventional volumetric measurement based assessment methods have been widely used in fish embryo membrane...