Rajendra B. Krishnan

Direct and crossover PCR amplification to facilitate Tn5supF-based sequencing of {lambda} phage clones (1991)

Krishnan, Rajendra B., Kersulyte, Dangeruta, Brikun, Igor, Berg, Claire M., Berg, Douglas E.

The 264 bp mini-transposon Tn5supF was constructed to sequence DNAs cloned in phage λ without extensive shotgun subcloning or primer walking. Unique sequences near each transposon end serve as...