The major subtypes of human B-cell lymphomas lack mutations in BCL-2 family member BAD (2006)
Schmitz, Roland, Thomas, Roman K., Harttrampf, Anne C., Wickenhauser, Claudia, Schultze, Joachim L., Hansmann, Martin-Leo, ...
Goossens, Tina, Klein, Ulf, Küppers, Ralf
Human naive and germinal center (GC) B cells were sorted by flow cytometry and rearranged VH region genes were amplified and sequenced from single cells. Whereas no deletions or insertions were found...
Isolation of viable Hodgkin and Reed-Sternberg cells from Hodgkin disease tissues
Irsch, Johannes, Nitsch, Silke, Hansmann, Martin-Leo, Rajewsky, Klaus, Tesch, Hans, Diehl, Volker, ...
Hodgkin disease (HD) is characterized by a small number of malignant Hodgkin and Reed-Sternberg (H/RS) cells among a major population of nonmalignant cells. The analysis of H/RS cells has been...
Pasqualucci, Laura, Migliazza, Anna, Fracchiolla, Nicola, William, Christopher, Neri, Antonino, Baldini, Luca, ...
The molecular mechanism involved in the process of antigen-driven somatic hypermutation of Ig genes is unknown, but it is commonly believed that this mechanism is restricted to the Ig loci. B cell...
Braeuninger, Andreas, Küppers, Ralf, Strickler, John G., Wacker, Hans-Heinrich, Rajewsky, Klaus, Hansmann, Martin-Leo
Among the four subtypes of Hodgkin disease (HD), lymphocyte-predominant (LP) HD is now generally considered as a separate entity. The B cell nature of the typical Hodgkin and Reed–Sternberg (HRS)...
Identification of Hodgkin and Reed-Sternberg cell-specific genes by gene expression profiling
Küppers, Ralf, Klein, Ulf, Schwering, Ines, Distler, Verena, Bräuninger, Andreas, Cattoretti, Giorgio, ...
Hodgkin lymphoma (HL) is a malignancy of unknown pathogenesis. The malignant Hodgkin and Reed/Sternberg (HRS) cells derive from germinal center B cells (or rarely, T cells) but have a heterogeneous...
Kurth, Julia, Hansmann, Martin-Leo, Rajewsky, Klaus, Küppers, Ralf
To assess the impact of the germinal center (GC) reaction on viral spread in Epstein–Barr virus (EBV) infection, we isolated EBV+ GC B cells from the tonsils of two infectious mononucleosis...
Schwering, Ines, Bräuninger, Andreas, Distler, Verena, Jesdinsky, Julia, Diehl, Volker, Hansmann, Martin-Leo, ...
The malignant cells of classical Hodgkin’s lymphoma (cHL), Hodgkin and Reed-Sternberg (HRS) cells, appear to be derived from germinal center (GC) B cells in most cases of the disease. Apart from...
Goossens, Tina, Klein, Ulf, Küppers, Ralf
Human naive and germinal center (GC) B cells were sorted by flow cytometry and rearranged VH region genes were amplified and sequenced from single cells. Whereas no deletions or insertions were found...
Isolation of viable Hodgkin and Reed-Sternberg cells from Hodgkin disease tissues
Irsch, Johannes, Nitsch, Silke, Hansmann, Martin-Leo, Rajewsky, Klaus, Tesch, Hans, Diehl, Volker, ...
Hodgkin disease (HD) is characterized by a small number of malignant Hodgkin and Reed-Sternberg (H/RS) cells among a major population of nonmalignant cells. The analysis of H/RS cells has been...
Pasqualucci, Laura, Migliazza, Anna, Fracchiolla, Nicola, William, Christopher, Neri, Antonino, Baldini, Luca, ...
The molecular mechanism involved in the process of antigen-driven somatic hypermutation of Ig genes is unknown, but it is commonly believed that this mechanism is restricted to the Ig loci. B cell...
Braeuninger, Andreas, Küppers, Ralf, Strickler, John G., Wacker, Hans-Heinrich, Rajewsky, Klaus, Hansmann, Martin-Leo
Among the four subtypes of Hodgkin disease (HD), lymphocyte-predominant (LP) HD is now generally considered as a separate entity. The B cell nature of the typical Hodgkin and Reed–Sternberg (HRS)...
Identification of Hodgkin and Reed-Sternberg cell-specific genes by gene expression profiling
Küppers, Ralf, Klein, Ulf, Schwering, Ines, Distler, Verena, Bräuninger, Andreas, Cattoretti, Giorgio, ...
Hodgkin lymphoma (HL) is a malignancy of unknown pathogenesis. The malignant Hodgkin and Reed/Sternberg (HRS) cells derive from germinal center B cells (or rarely, T cells) but have a heterogeneous...
Kurth, Julia, Hansmann, Martin-Leo, Rajewsky, Klaus, Küppers, Ralf
To assess the impact of the germinal center (GC) reaction on viral spread in Epstein–Barr virus (EBV) infection, we isolated EBV+ GC B cells from the tonsils of two infectious mononucleosis...
Schwering, Ines, Bräuninger, Andreas, Distler, Verena, Jesdinsky, Julia, Diehl, Volker, Hansmann, Martin-Leo, ...
The malignant cells of classical Hodgkin’s lymphoma (cHL), Hodgkin and Reed-Sternberg (HRS) cells, appear to be derived from germinal center (GC) B cells in most cases of the disease. Apart from...
Absence of Immunoglobulin Class Switch in Primary Lymphomas of the Central Nervous System
Montesinos-Rongen, Manuel, Schmitz, Roland, Courts, Cornelius, Stenzel, Werner, Bechtel, Dörte, Niedobitek, Gerald, ...
Primary lymphomas of the central nervous system (PCNSLs) were investigated for their capacity to perform further maturation steps. We studied a series of 11 PCNSLs derived from immunocompetent...
Aberrant Expression of ID2, a Suppressor of B-Cell-Specific Gene Expression, in Hodgkin’s Lymphoma
Renné, Christoph, Martin-Subero, Jose Ignacio, Eickernjäger, Maren, Hansmann, Martin-Leo, Küppers, Ralf, Siebert, Reiner, ...
The global loss of B-cell-specific gene expression is a distinctive feature of the Hodgkin-Reed/Sternberg (HRS) cells of classical Hodgkin’s lymphoma (HL). The reasons for this loss remained...
Single-Cell PCR Analysis of T Helper Cells in Human Lymph Node Germinal Centers
Roers, Axel, Hansmann, Martin Leo, Rajewsky, Klaus, Küppers, Ralf
The T helper cell population of human lymph node germinal centers (GCs) was analyzed for clonality and signs of antigen selection. Frozen sections of lymph node biopsies taken from three different...
Montesinos-Rongen, Manuel, Küppers, Ralf, Schlüter, Dirk, Spieker, Tilmann, Van Roost, Dirk, Schaller, Carlo, ...
Primary central nervous system lymphomas (PCNSLs) have recently received considerable clinical attention due to their increasing incidence. To clarify the histogenetic origin of these intriguing...
Willenbrock, Klaus, Roers, Axel, Seidl, Christian, Wacker, Hans-Heinrich, Küppers, Ralf, Hansmann, Martin-Leo
Angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) is defined in the current lymphoma classifications as a T-cell non-Hodgkin’s lymphoma. However, in approximately one third of the cases...
Silencing of B Cell Receptor Signals in Human Naive B Cells
Feldhahn, Niklas, Schwering, Ines, Lee, Sanggyu, Wartenberg, Maria, Klein, Florian, Wang, Hui, ...
To identify changes in the regulation of B cell receptor (BCR) signals during the development of human B cells, we generated genome-wide gene expression profiles using the serial analysis of gene...
Bräuninger, Andreas, Spieker, Tilmann, Willenbrock, Klaus, Gaulard, Philippe, Wacker, Hans-Heinrich, Rajewsky, Klaus, ...
Angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) is a peculiar T cell lymphoma, as expanding B cell clones are often present besides the malignant T cell clones. In addition, large...
Clonal Deleterious Mutations in the Iκbα Gene in the Malignant Cells in Hodgkin's Lymphoma
Jungnickel, Berit, Staratschek-Jox, Andrea, Bräuninger, Andreas, Spieker, Tilmann, Wolf, Jürgen, Diehl, Volker, ...
Members of the nuclear factor (NF)-κB family of transcription factors play a crucial role in cellular activation, immune responses, and oncogenesis. In most cells, they are kept inactive in the...
Rare Occurrence of Classical Hodgkin's Disease as a T Cell Lymphoma
Müschen, Markus, Rajewsky, Klaus, Bräuninger, Andreas, Baur, Audrey Sylvia, Oudejans, Joost J., Roers, Axel, ...
Recent work identified Hodgkin and Reed-Sternberg (H/RS) cells in classical Hodgkin's disease (cHD) as clonal progeny of mature B cells. Therefore, it is generally assumed that cHD homogenously...
Klein, Ulf, Rajewsky, Klaus, Küppers, Ralf
Immunoglobulin (Ig)M+IgD+ B cells are generally assumed to represent antigen-inexperienced, naive B cells expressing variable (V) region genes without somatic mutations. We report here that human...
Somatic Mutation of the Cd95 Gene in Human B Cells as a Side-Effect of the Germinal Center Reaction
Müschen, Markus, Re, Daniel, Jungnickel, Berit, Diehl, Volker, Rajewsky, Klaus, Küppers, Ralf
Somatic hypermutation specifically modifies rearranged immunoglobulin (Ig) genes in germinal center (GC) B cells. However, the bcl-6 gene can also acquire somatic mutations during the GC reaction,...
Brune, Verena, Tiacci, Enrico, Pfeil, Ines, Döring, Claudia, Eckerle, Susan, ...
The pathogenesis of nodular lymphocyte–predominant Hodgkin lymphoma (NLPHL) and its relationship to other lymphomas are largely unknown. This is partly because of the technical challenge of...
A Comprehensive Microarray-Based DNA Methylation Study of 367 Hematological Neoplasms
Martin-Subero, Jose I., Ammerpohl, Ole, Bibikova, Marina, Wickham-Garcia, Eliza, Agirre, Xabier, Alvarez, Sara, ...
TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma
Schmitz, Roland, Hansmann, Martin-Leo, Bohle, Verena, Martin-Subero, Jose Ignacio, Hartmann, Sylvia, Mechtersheimer, Gunhild, ...
Proliferation and survival of Hodgkin and Reed/Sternberg (HRS) cells, the malignant cells of classical Hodgkin lymphoma (cHL), are dependent on constitutive activation of nuclear factor κB (NF-κB)....