Marion S. Dorer, Donald Kirton, Joel S. Bader, Ralph R. Isberg
Legionella pneumophila translocates multiple bacterial effector proteins into host cells to direct formation of a replication vacuole for the bacterium. The emerging consensus is that formation of...
Yersinia pseudotuberculosis Spatially Controls Activation and Misregulation of Host Cell Rac1 (2005)
Yersinia pseudotuberculosis binds host cells and modulates the mammalian Rac1 guanosine triphosphatase (GTPase) at two levels. Activation of Rac1 results from integrin receptor engagement, while...
Mechanism and regulation of Tn5 transposition in Escherichia coli [microform] / (1983)
Thesis (Ph. D.)--Harvard University, 1984.
Mechanism and regulation of Tn5 transposition in Escherichia coli /--by Ralph R. Isberg. (1983)
Thesis (Ph. D.)--Harvard University, 1984.
Involvement of focal adhesion kinase in invasin-mediated uptake
Alrutz, Michael A., Isberg, Ralph R.
High-efficiency entry of the enteropathogenic bacterium Yersinia pseudotuberculosis into nonphagocytic cells is mediated by the bacterial outer membrane protein invasin. Invasin-mediated uptake...
Dersch, Petra, Isberg, Ralph R.
The binding of the Yersinia pseudotuberculosis and Yersinia enterocolitica invasin proteins to β1 integrin receptors allows internalization of these organisms by cultured cells. The C-terminal...
Solomon, Jonathan M., Rupper, Adam, Cardelli, James A., Isberg, Ralph R.
Conditions were established in which Legionella pneumophila, an intracellular bacterial pathogen, could replicate within the unicellular organism Dictyostelium discoideum. By several criteria, L....
Andrews, Helene L., Vogel, Joseph P., Isberg, Ralph R.
Legionella pneumophila replicates within a specialized phagosome in cultured cells, a function necessary for its pathogenicity. The replicative phagosome lacks membrane marker proteins, such as the...
Rankin, Susannah, Li, Zhiru, Isberg, Ralph R.
A promoter-probe strategy was devised to identify genes specifically expressed by Legionella pneumophila during growth within the macrophage. Random fragments from the L. pneumophila chromosome were...
Solomon, Jonathan M., Leung, Grace S., Isberg, Ralph R.
Mycobacterium marinum causes tuberculosis-like disease in fish and amphibians and has been used as a model mycobacterial species because of its rapid growth and less stringent containment...
Luo, Zhao-Qing, Isberg, Ralph R.
Legionella pneumophila is an intracellular pathogen that multiplies in a specialized vacuole within host cells. Biogenesis of this vacuole requires the Dot/Icm type IV protein translocation system....
Derré, Isabelle, Isberg, Ralph R.
Legionella pneumophila vacuole biogenesis was analyzed by using a cell-free system. We show that calnexin, Sec22b, and Rab1 are recruited to the vacuole very shortly after bacterial uptake, and we...
VanRheenen, Susan M., Duménil, Guillaume, Isberg, Ralph R.
The gram-negative bacterium Legionella pneumophila causes a severe form of pneumonia called Legionnaires' disease, characterized by bacterial replication within alveolar macrophages. Prior to...
Derré, Isabelle, Isberg, Ralph R.
Although Legionella pneumophila can multiply in diverse cell types from a variety of species, macrophages from most inbred mouse strains are nonpermissive for intracellular replication and allow...
Buscher, Benjamin A., Conover, Gloria M., Miller, Jennifer L., Vogel, Sinae A., Meyers, Stacey N., Isberg, Ralph R., ...
Legionella pneumophila is able to survive inside phagocytic cells by an internalization route that bypasses fusion of the nascent phagosome with the endocytic pathway to allow formation of a...
Yersinia pseudotuberculosis Spatially Controls Activation and Misregulation of Host Cell Rac1
Wong, Ka-Wing, Isberg, Ralph R
Yersinia pseudotuberculosis binds host cells and modulates the mammalian Rac1 guanosine triphosphatase (GTPase) at two levels. Activation of Rac1 results from integrin receptor engagement, while...
Derré, Isabelle, Isberg, Ralph R.
Legionella pneumophila uses a type IV secretion system to deliver effector molecules into the host cell and establish its replication vacuole. In this study, we investigated the role of LidA, a...
Dorer, Marion S, Kirton, Donald, Bader, Joel S, Isberg, Ralph R
Legionella pneumophila translocates multiple bacterial effector proteins into host cells to direct formation of a replication vacuole for the bacterium. The emerging consensus is that formation of...
VanRheenen, Susan M., Luo, Zhao-Qing, O'Connor, Tamara, Isberg, Ralph R.
Legionella pneumophila replicates within alveolar macrophages, causing a severe pneumonia termed Legionnaires' disease. The bacterium resides within a vacuole that escapes immediate transport to the...
Involvement of focal adhesion kinase in invasin-mediated uptake
Alrutz, Michael A., Isberg, Ralph R.
High-efficiency entry of the enteropathogenic bacterium Yersinia pseudotuberculosis into nonphagocytic cells is mediated by the bacterial outer membrane protein invasin. Invasin-mediated uptake...
Dersch, Petra, Isberg, Ralph R.
The binding of the Yersinia pseudotuberculosis and Yersinia enterocolitica invasin proteins to β1 integrin receptors allows internalization of these organisms by cultured cells. The C-terminal...
Solomon, Jonathan M., Rupper, Adam, Cardelli, James A., Isberg, Ralph R.
Conditions were established in which Legionella pneumophila, an intracellular bacterial pathogen, could replicate within the unicellular organism Dictyostelium discoideum. By several criteria, L....
Andrews, Helene L., Vogel, Joseph P., Isberg, Ralph R.
Legionella pneumophila replicates within a specialized phagosome in cultured cells, a function necessary for its pathogenicity. The replicative phagosome lacks membrane marker proteins, such as the...
Rankin, Susannah, Li, Zhiru, Isberg, Ralph R.
A promoter-probe strategy was devised to identify genes specifically expressed by Legionella pneumophila during growth within the macrophage. Random fragments from the L. pneumophila chromosome were...
Solomon, Jonathan M., Leung, Grace S., Isberg, Ralph R.
Mycobacterium marinum causes tuberculosis-like disease in fish and amphibians and has been used as a model mycobacterial species because of its rapid growth and less stringent containment...
Luo, Zhao-Qing, Isberg, Ralph R.
Legionella pneumophila is an intracellular pathogen that multiplies in a specialized vacuole within host cells. Biogenesis of this vacuole requires the Dot/Icm type IV protein translocation system....
Derré, Isabelle, Isberg, Ralph R.
Legionella pneumophila vacuole biogenesis was analyzed by using a cell-free system. We show that calnexin, Sec22b, and Rab1 are recruited to the vacuole very shortly after bacterial uptake, and we...
VanRheenen, Susan M., Duménil, Guillaume, Isberg, Ralph R.
The gram-negative bacterium Legionella pneumophila causes a severe form of pneumonia called Legionnaires' disease, characterized by bacterial replication within alveolar macrophages. Prior to...
Derré, Isabelle, Isberg, Ralph R.
Although Legionella pneumophila can multiply in diverse cell types from a variety of species, macrophages from most inbred mouse strains are nonpermissive for intracellular replication and allow...
Buscher, Benjamin A., Conover, Gloria M., Miller, Jennifer L., Vogel, Sinae A., Meyers, Stacey N., Isberg, Ralph R., ...
Legionella pneumophila is able to survive inside phagocytic cells by an internalization route that bypasses fusion of the nascent phagosome with the endocytic pathway to allow formation of a...
Derré, Isabelle, Isberg, Ralph R.
Legionella pneumophila uses a type IV secretion system to deliver effector molecules into the host cell and establish its replication vacuole. In this study, we investigated the role of LidA, a...
Yersinia pseudotuberculosis Spatially Controls Activation and Misregulation of Host Cell Rac1
Wong, Ka-Wing, Isberg, Ralph R
Yersinia pseudotuberculosis binds host cells and modulates the mammalian Rac1 guanosine triphosphatase (GTPase) at two levels. Activation of Rac1 results from integrin receptor engagement, while...
Dorer, Marion S, Kirton, Donald, Bader, Joel S, Isberg, Ralph R
Legionella pneumophila translocates multiple bacterial effector proteins into host cells to direct formation of a replication vacuole for the bacterium. The emerging consensus is that formation of...
VanRheenen, Susan M., Luo, Zhao-Qing, O'Connor, Tamara, Isberg, Ralph R.
Legionella pneumophila replicates within alveolar macrophages, causing a severe pneumonia termed Legionnaires' disease. The bacterium resides within a vacuole that escapes immediate transport to the...
Laguna, Rita K., Creasey, Elizabeth A., Li, Zhiru, Valtz, Nicole, Isberg, Ralph R.
Legionella pneumophila requires the Dot/Icm protein translocation system to replicate within host cells as a critical component of Legionnaire's pneumonia. None of the known individual substrates of...
Auerbuch, Victoria, Isberg, Ralph R.
Pathogenic Yersinia translocates effector proteins into target cells via a type III secretion system (TTSS), modulating the host immune response. A component of the TTSS translocon, LcrV, has been...
Yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine
Barnes, Penelope D., Bergman, Molly A., Mecsas, Joan, Isberg, Ralph R.
Dissemination of Yersinia pseudotuberculosis within mice after oral inoculation was analyzed. Y. pseudotuberculosis translocated to organs such as the liver and spleen shortly after oral inoculation,...
NF-κB translocation prevents host cell death after low-dose challenge by Legionella pneumophila
Losick, Vicki P., Isberg, Ralph R.
Legionella pneumophila, the causative agent of Legionnaires' disease, grows within macrophages and manipulates target cell signaling. Formation of a Legionella-containing replication vacuole requires...
Wong, Ka-Wing, Isberg, Ralph R.
Efficient entry of the bacterium Yersinia pseudotuberculosis into mammalian cells requires the binding of the bacterial invasin protein to β1 integrin receptors and the activation of the small...
Watarai, Masahisa, Derre, Isabelle, Kirby, James, Growney, Joseph D., Dietrich, William F., Isberg, Ralph R.
The products of the Legionella pneumophila dot/icm genes enable the bacterium to replicate within a macrophage vacuole. This study demonstrates that the Dot/Icm machinery promotes macropinocytotic...
Shin, Ok S., Isberg, Ralph R., Akira, Shizuo, Uematsu, Satoshi, Behera, Aruna K., Hu, Linden T.
The contribution of Toll-like receptors (TLRs) to phagocytosis of Borrelia burgdorferi has not been extensively studied. We show that bone marrow-derived macrophages (BMDM) from MyD88−/− mice or...
Wong, Ka-Wing, Mohammadi, Sina, Isberg, Ralph R.
The COOH-terminal polybasic region (PBR) of Rac1, a Rho family GTPase member, is required for Rac1 self-association, membrane localization, nuclear translocation, and interaction with downstream...
Losick, Vicki P., Stephan, Kristin, Smirnova, Irina I., Isberg, Ralph R., Poltorak, Alexander
Mouse-derived macrophages have the unique ability to restrict or permit Legionella pneumophila intracellular growth. The common inbred mouse strain C57BL/6J (B6) restricts L. pneumophila growth,...
Bergman, Molly A., Loomis, Wendy P., Mecsas, Joan, Starnbach, Michael N., Isberg, Ralph R.
All Yersinia species target and bind to phagocytic cells, but uptake and destruction of bacteria are prevented by injection of anti-phagocytic Yop proteins into the host cell. Here we provide...