Renée M. Tsolis

Ferrioxamine-Mediated Iron(III) Utilization by Salmonella enterica

Kingsley, Robert A., Reissbrodt, Rolf, Rabsch, Wolfgang, Ketley, Julian M., Tsolis, Renée M., Everest, Paul, ...

Utilization of ferrioxamines as sole sources of iron distinguishes Salmonella enterica serotypes Typhimurium and Enteritidis from a number of related species, including Escherichia coli. Ferrioxamine...

Salmonella typhimurium IroN and FepA Proteins Mediate Uptake of Enterobactin but Differ in Their Specificity for Other Siderophores

Rabsch, Wolfgang, Voigt, Wolfgang, Reissbrodt, Rolf, Tsolis, Renée M., Bäumler, Andreas J.

Salmonella typhimurium possesses two outer membrane receptor proteins, IroN and FepA, which have been implicated in the uptake of enterobactin. To determine whether both receptors have identical...

Contribution of Salmonella typhimurium Virulence Factors to Diarrheal Disease in Calves

Tsolis, Renée M., Adams, L. Garry, Ficht, Thomas A., Bäumler, Andreas J.

Limited knowledge is available about the virulence mechanisms responsible for diarrheal disease caused by Salmonella typhimurium. To assess the contribution to diarrheal disease of virulence...

Identification of a Putative Salmonella enterica Serotype Typhimurium Host Range Factor with Homology to IpaH and YopM by Signature-Tagged Mutagenesis

Tsolis, Renée M., Townsend, Stacy M., Miao, Edward A., Miller, Samuel I., Ficht, Thomas A., Adams, L. Garry, ...

The genetic basis for the host adaptation of Salmonella serotypes is currently unknown. We have explored a new strategy to identify Salmonella enterica serotype Typhimurium (S. typhimurium) genes...

SspA Is Required for Lethal Salmonella enterica Serovar Typhimurium Infections in Calves but Is Not Essential for Diarrhea

Tsolis, Renée M., Adams, L. Garry, Hantman, Michael J., Scherer, Christina A., Kimbrough, Tyler, Kingsley, Robert A., ...

Salmonella pathogenicity island 1 (SPI-1) encodes virulence determinants, which are important for enteropathogenicity in calves. To determine whether the Salmonella enterica serovar Typhimurium SPI-1...

Salmonella enterica Serovar Typhimurium Induces Cell Death in Bovine Monocyte-Derived Macrophages by Early sipB-Dependent and Delayed sipB-Independent Mechanisms

Santos, Renato L., Tsolis, Renée M., Bäumler, Andreas J., Smith, Roger, Adams, L. Garry

It was previously demonstrated that Salmonella enterica serovar Typhimurium induces cell death with features of apoptosis in murine macrophages. Mice infected with Salmonella serovar Typhimurium...

Salmonella-Induced Cell Death Is Not Required for Enteritis in Calves

Santos, Renato L., Tsolis, Renée M., Zhang, Shuping, Ficht, Thomas A., Bäumler, Andreas J., Adams, L. Garry

Salmonella enterica serovar Typhimurium causes cell death in bovine monocyte-derived and murine macrophages in vitro by a sipB-dependent mechanism. During this process, SipB binds and activates...

Identification of Genes Required for Chronic Persistence of Brucella abortus in Mice

Hong, Priscilla C., Tsolis, Renée M., Ficht, Thomas A.

The genetic basis for chronic persistence of Brucella abortus in lymphoid organs of mice, cows, and humans is currently unknown. We identified B. abortus genes involved in chronic infection, by...

Multiple Fimbrial Adhesins Are Required for Full Virulence of Salmonella typhimurium in Mice

Bäumler, Andreas J., Tsolis, Renée M., Heffron, Fred

Adhesion is an important initial step during bacterial colonization of the intestinal mucosa. However, mutations in the Salmonella typhimurium fimbrial operons lpf, pef, or fim only moderately alter...

virB-Mediated Survival of Brucella abortus in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

Sun, Yao-Hui, Den Hartigh, Andreas B., De Lima Santos, Renato, Adams, L. Garry, Tsolis, Renée M.

The Brucella abortus virB locus is required for establishing chronic infection in the mouse. Using in vitro and in vivo models, we investigated whether virB is involved in evasion of the bactericidal...

Secreted Effector Proteins of Salmonella enterica Serotype Typhimurium Elicit Host-Specific Chemokine Profiles in Animal Models of Typhoid Fever and Enterocolitis

Zhang, Shuping, Adams, L. Garry, Nunes, Jairo, Khare, Sangeeta, Tsolis, Renée M., Bäumler, Andreas J.

Infection of bovine ligated loops with the Salmonella enterica serotype Typhimurium wild type but not a sipA sopABDE2 mutant resulted in fluid accumulation, polymorphonuclear cell infiltration, and...

Differential Requirements for VirB1 and VirB2 during Brucella abortus Infection

Den Hartigh, Andreas B., Sun, Yao-Hui, Sondervan, David, Heuvelmans, Niki, Reinders, Marjolein O., Ficht, Thomas A., ...

The Brucella abortus virB operon, encoding a type IV secretion system (T4SS), is required for intracellular replication and persistent infection in the mouse model. The products of the first two...

The Salmonella enterica Serotype Typhimurium lpf, bcf, stb, stc, std, and sth Fimbrial Operons Are Required for Intestinal Persistence in Mice

Weening, Eric H., Barker, Jared D., Laarakker, Marijke C., Humphries, Andrea D., Tsolis, Renée M., Bäumler, Andreas J.

Salmonella enterica serotype Typhimurium causes human infections that can frequently be traced back through the food chain to healthy livestock whose intestine is colonized by the pathogen. Little is...

Brucella abortus VirB12 Is Expressed during Infection but Is Not an Essential Component of the Type IV Secretion System

Sun, Yao-Hui, Rolán, Hortensia G., Den Hartigh, Andreas B., Sondervan, David, Tsolis, Renée M.

The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, orf12 (virB12) and orf13, encodes a type IV secretion system (T4SS) that is required for...

Host Restriction of Salmonella enterica Serotype Typhi Is Not Caused by Functional Alteration of SipA, SopB, or SopD

Raffatellu, Manuela, Sun, Yao-Hui, Wilson, R. Paul, Tran, Quynh T., Chessa, Daniela, Andrews-Polymenis, Helene L., ...

Salmonella enterica serotype Typhi is a strictly human adapted pathogen that does not cause disease in nonprimate vertebrate hosts, while Salmonella enterica serotype Typhimurium is a...

RecA and RadA Proteins of Brucella abortus Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst

Roux, Christelle M., Booth, Natha J., Bellaire, Bryan H., Gee, Jason M., Roop, R. Martin, Kovach, Michael E., ...

Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus....

Ferrioxamine-Mediated Iron(III) Utilization by Salmonella enterica

Kingsley, Robert A., Reissbrodt, Rolf, Rabsch, Wolfgang, Ketley, Julian M., Tsolis, Renée M., Everest, Paul, ...

Utilization of ferrioxamines as sole sources of iron distinguishes Salmonella enterica serotypes Typhimurium and Enteritidis from a number of related species, including Escherichia coli. Ferrioxamine...

Salmonella typhimurium IroN and FepA Proteins Mediate Uptake of Enterobactin but Differ in Their Specificity for Other Siderophores

Rabsch, Wolfgang, Voigt, Wolfgang, Reissbrodt, Rolf, Tsolis, Renée M., Bäumler, Andreas J.

Salmonella typhimurium possesses two outer membrane receptor proteins, IroN and FepA, which have been implicated in the uptake of enterobactin. To determine whether both receptors have identical...

Contribution of Salmonella typhimurium Virulence Factors to Diarrheal Disease in Calves

Tsolis, Renée M., Adams, L. Garry, Ficht, Thomas A., Bäumler, Andreas J.

Limited knowledge is available about the virulence mechanisms responsible for diarrheal disease caused by Salmonella typhimurium. To assess the contribution to diarrheal disease of virulence...

Identification of a Putative Salmonella enterica Serotype Typhimurium Host Range Factor with Homology to IpaH and YopM by Signature-Tagged Mutagenesis

Tsolis, Renée M., Townsend, Stacy M., Miao, Edward A., Miller, Samuel I., Ficht, Thomas A., Adams, L. Garry, ...

The genetic basis for the host adaptation of Salmonella serotypes is currently unknown. We have explored a new strategy to identify Salmonella enterica serotype Typhimurium (S. typhimurium) genes...

SspA Is Required for Lethal Salmonella enterica Serovar Typhimurium Infections in Calves but Is Not Essential for Diarrhea

Tsolis, Renée M., Adams, L. Garry, Hantman, Michael J., Scherer, Christina A., Kimbrough, Tyler, Kingsley, Robert A., ...

Salmonella pathogenicity island 1 (SPI-1) encodes virulence determinants, which are important for enteropathogenicity in calves. To determine whether the Salmonella enterica serovar Typhimurium SPI-1...

Salmonella enterica Serovar Typhimurium Induces Cell Death in Bovine Monocyte-Derived Macrophages by Early sipB-Dependent and Delayed sipB-Independent Mechanisms

Santos, Renato L., Tsolis, Renée M., Bäumler, Andreas J., Smith, Roger, Adams, L. Garry

It was previously demonstrated that Salmonella enterica serovar Typhimurium induces cell death with features of apoptosis in murine macrophages. Mice infected with Salmonella serovar Typhimurium...

Salmonella-Induced Cell Death Is Not Required for Enteritis in Calves

Santos, Renato L., Tsolis, Renée M., Zhang, Shuping, Ficht, Thomas A., Bäumler, Andreas J., Adams, L. Garry

Salmonella enterica serovar Typhimurium causes cell death in bovine monocyte-derived and murine macrophages in vitro by a sipB-dependent mechanism. During this process, SipB binds and activates...

Identification of Genes Required for Chronic Persistence of Brucella abortus in Mice

Hong, Priscilla C., Tsolis, Renée M., Ficht, Thomas A.

The genetic basis for chronic persistence of Brucella abortus in lymphoid organs of mice, cows, and humans is currently unknown. We identified B. abortus genes involved in chronic infection, by...

Multiple Fimbrial Adhesins Are Required for Full Virulence of Salmonella typhimurium in Mice

Bäumler, Andreas J., Tsolis, Renée M., Heffron, Fred

Adhesion is an important initial step during bacterial colonization of the intestinal mucosa. However, mutations in the Salmonella typhimurium fimbrial operons lpf, pef, or fim only moderately alter...

virB-Mediated Survival of Brucella abortus in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

Sun, Yao-Hui, Den Hartigh, Andreas B., De Lima Santos, Renato, Adams, L. Garry, Tsolis, Renée M.

The Brucella abortus virB locus is required for establishing chronic infection in the mouse. Using in vitro and in vivo models, we investigated whether virB is involved in evasion of the bactericidal...

Secreted Effector Proteins of Salmonella enterica Serotype Typhimurium Elicit Host-Specific Chemokine Profiles in Animal Models of Typhoid Fever and Enterocolitis

Zhang, Shuping, Adams, L. Garry, Nunes, Jairo, Khare, Sangeeta, Tsolis, Renée M., Bäumler, Andreas J.

Infection of bovine ligated loops with the Salmonella enterica serotype Typhimurium wild type but not a sipA sopABDE2 mutant resulted in fluid accumulation, polymorphonuclear cell infiltration, and...

Differential Requirements for VirB1 and VirB2 during Brucella abortus Infection

Den Hartigh, Andreas B., Sun, Yao-Hui, Sondervan, David, Heuvelmans, Niki, Reinders, Marjolein O., Ficht, Thomas A., ...

The Brucella abortus virB operon, encoding a type IV secretion system (T4SS), is required for intracellular replication and persistent infection in the mouse model. The products of the first two...

The Salmonella enterica Serotype Typhimurium lpf, bcf, stb, stc, std, and sth Fimbrial Operons Are Required for Intestinal Persistence in Mice

Weening, Eric H., Barker, Jared D., Laarakker, Marijke C., Humphries, Andrea D., Tsolis, Renée M., Bäumler, Andreas J.

Salmonella enterica serotype Typhimurium causes human infections that can frequently be traced back through the food chain to healthy livestock whose intestine is colonized by the pathogen. Little is...

Brucella abortus VirB12 Is Expressed during Infection but Is Not an Essential Component of the Type IV Secretion System

Sun, Yao-Hui, Rolán, Hortensia G., Den Hartigh, Andreas B., Sondervan, David, Tsolis, Renée M.

The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, orf12 (virB12) and orf13, encodes a type IV secretion system (T4SS) that is required for...

Host Restriction of Salmonella enterica Serotype Typhi Is Not Caused by Functional Alteration of SipA, SopB, or SopD

Raffatellu, Manuela, Sun, Yao-Hui, Wilson, R. Paul, Tran, Quynh T., Chessa, Daniela, Andrews-Polymenis, Helene L., ...

Salmonella enterica serotype Typhi is a strictly human adapted pathogen that does not cause disease in nonprimate vertebrate hosts, while Salmonella enterica serotype Typhimurium is a...

RecA and RadA Proteins of Brucella abortus Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst

Roux, Christelle M., Booth, Natha J., Bellaire, Bryan H., Gee, Jason M., Roop, R. Martin, Kovach, Michael E., ...

Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus....

MarT Activates Expression of the MisL Autotransporter Protein of Salmonella enterica Serotype Typhimurium▿

Tükel, Çagla, Akçelik, Mustafa, De Jong, Maarten F., Şimşek, Ömer, Tsolis, Renée M., Bäumler, Andreas J.

MisL is a Salmonella enterica serotype Typhimurium fibronectin binding protein whose expression is induced during infection of mice. T-POP transposon mutagenesis identified marT as a positive...

Mice Lacking Components of Adaptive Immunity Show Increased Brucella abortus virB Mutant Colonization▿

Rolán, Hortensia García, Tsolis, Renée M.

The Brucella abortus type IV secretion system (T4SS), encoded by the virB genes, is essential for survival in mononuclear phagocytes in vitro. In the mouse model, a B. abortus virB mutant was...

VirB12 Is a Serological Marker of Brucella Infection in Experimental and Natural Hosts▿

Rolán, Hortensia G., Den Hartigh, Andreas B., Kahl-McDonagh, Melissa, Ficht, Thomas, Adams, L. Garry, Tsolis, Renée M.

The Brucella species type IV secretion system, encoded by the virB1-12 locus, is required for intracellular replication and persistent infection in vivo. The requirement of VirB proteins for...

Modulation of the Bovine Trophoblastic Innate Immune Response by Brucella abortus▿

Carvalho Neta, Alcina V., Stynen, Ana P. R., Paixão, Tatiane A., Miranda, Karina L., Silva, Fabiana L., Roux, Christelle M., ...

Brucellosis is still a widespread zoonotic disease. Very little is known about the interaction between Brucella abortus and trophoblastic cells, which is essential for better understanding the...

T Cells Help To Amplify Inflammatory Responses Induced by Salmonella enterica Serotype Typhimurium in the Intestinal Mucosa▿

Godinez, Ivan, Haneda, Takeshi, Raffatellu, Manuela, George, Michael D., Paixão, Tatiane A., Rolán, Hortensia G., ...

Salmonella enterica serotype Typhimurium causes an acute inflammatory reaction in the ceca of streptomycin-pretreated mice. We determined global changes in gene expression elicited by serotype...

Inactivation of the Type IV Secretion System Reduces the Th1 Polarization of the Immune Response to Brucella abortus Infection▿

Rolán, Hortensia García, Tsolis, Renée M.

The Brucella abortus type IV secretion system (T4SS), encoded by the virB operon, is essential for establishing persistent infection in the murine reticuloendothelial system. To gain insight into the...

VirB3 to VirB6 and VirB8 to VirB11, but Not VirB7, Are Essential for Mediating Persistence of Brucella in the Reticuloendothelial System▿

Den Hartigh, Andreas B., Rolán, Hortensia G., De Jong, Maarten F., Tsolis, Renée M.

The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of...