Renate Kunert

A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits (2008)

Jin, Chunsheng, Altmann, Friedrich, Strasser, Richard, Mach, Lukas, Schähs, Matthias, Kunert, Renate, ...

A common argument against using plants as a production system for therapeutic proteins is their inability to perform authentic N-glycosylation. A major concern is the presence of beta 1,2-xylose and...

Characterization of Human Class-Switched Polymeric (Immunoglobulin M [IgM] and IgA) Anti-Human Immunodeficiency Virus Type 1 Antibodies 2F5 and 2G12

Wolbank, Susanne, Kunert, Renate, Stiegler, Gabriela, Katinger, Hermann

We have previously generated human monoclonal anti-human immunodeficiency virus type 1 (anti-HIV-1) antibodies 2F5IgG and 2G12IgG with an exceptional cross-clade neutralizing potential. 2F5IgG and...

The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2F5

Zwick, Michael B., Komori, H. Kiyomi, Stanfield, Robyn L., Church, Sarah, Wang, Meng, ...

The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to...

Inhibition of Human Immunodeficiency Virus Type 1 Entry in Cells Expressing gp41-Derived Peptides

Egelhofer, Marc, Brandenburg, Gunda, Martinius, Holger, Schult-Dietrich, Patricia, Melikyan, Gregory, Kunert, Renate, ...

As the limitations of antiretroviral drug therapy, such as toxicity and resistance, become evident, interest in alternative therapeutic approaches for human immunodeficiency virus (HIV) infection is...

Binding of the 2F5 Monoclonal Antibody to Native and Fusion-Intermediate Forms of Human Immunodeficiency Virus Type 1 gp41: Implications for Fusion-Inducing Conformational Changes

De Rosny, Eve, Vassell, Russell, Jiang, Shibo, Kunert, Renate, Weiss, Carol D.

We investigated how the broadly neutralizing monoclonal antibody 2F5 affects the human immunodeficiency virus type 1 envelope glycoprotein as it undergoes receptor-induced conformational changes and...

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1

Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...

The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...

Characterization of Human Class-Switched Polymeric (Immunoglobulin M [IgM] and IgA) Anti-Human Immunodeficiency Virus Type 1 Antibodies 2F5 and 2G12

Wolbank, Susanne, Kunert, Renate, Stiegler, Gabriela, Katinger, Hermann

We have previously generated human monoclonal anti-human immunodeficiency virus type 1 (anti-HIV-1) antibodies 2F5IgG and 2G12IgG with an exceptional cross-clade neutralizing potential. 2F5IgG and...

The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2F5

Zwick, Michael B., Komori, H. Kiyomi, Stanfield, Robyn L., Church, Sarah, Wang, Meng, ...

The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to...

Inhibition of Human Immunodeficiency Virus Type 1 Entry in Cells Expressing gp41-Derived Peptides

Egelhofer, Marc, Brandenburg, Gunda, Martinius, Holger, Schult-Dietrich, Patricia, Melikyan, Gregory, Kunert, Renate, ...

As the limitations of antiretroviral drug therapy, such as toxicity and resistance, become evident, interest in alternative therapeutic approaches for human immunodeficiency virus (HIV) infection is...

Binding of the 2F5 Monoclonal Antibody to Native and Fusion-Intermediate Forms of Human Immunodeficiency Virus Type 1 gp41: Implications for Fusion-Inducing Conformational Changes

De Rosny, Eve, Vassell, Russell, Jiang, Shibo, Kunert, Renate, Weiss, Carol D.

We investigated how the broadly neutralizing monoclonal antibody 2F5 affects the human immunodeficiency virus type 1 envelope glycoprotein as it undergoes receptor-induced conformational changes and...

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1

Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...

The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...

Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles

Reisinger, Hannes, Sevcsik, Eva, Vorauer-Uhl, Karola, Lohner, Karl, Katinger, Hermann, Kunert, Renate

At present, a number of transfection techniques are available to introduce foreign DNA into cells, but still minimal intrusion or interference with normal cell physiology, low toxicity,...

The Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 4E10 Monoclonal Antibody Is Better Adapted to Membrane-Bound Epitope Recognition and Blocking than 2F5▿ †

Huarte, Nerea, Lorizate, Maier, Maeso, Rubén, Kunert, Renate, Arranz, Rocio, Valpuesta, José M., ...

The broadly neutralizing 2F5 and 4E10 monoclonal antibodies (MAbs) recognize epitopes within the membrane-proximal external region (MPER) that connects the human immunodeficiency virus type 1 (HIV-1)...