Jaikaran, Dominic, Smith, M. Duane, Mehdizadeh, Reza, Olive, Joan, Collins, Richard A.
We describe a chemical coupling procedure that allows joining of two RNAs, one of which contains a site-specific base analog substitution, in the absence of divalent ions. This method allows...
The ionic environment determines ribozyme cleavage rate by modulation of nucleobase pK a (2008)
Smith, M. Duane, Mehdizadeh, Reza, Olive, Joan E., Collins, Richard A.
Several small ribozymes employ general acid–base catalysis as a mechanism to enhance site-specific RNA cleavage, even though the functional groups on the ribonucleoside building blocks of RNA have...
Kwan, Patrick, Johnston, Janice, Fung, Anne YK, Chong, Doris SY, Collins, Richard A, Lo, Su V
Abstract Background The Health and Health Services Research Fund (HHSRF) is dedicated to support research related to all aspects of health and health services in Hong Kong. We evaluated the fund's...
The contribution of 2'-hydroxyls to the cleavage activity of the Neurospora VS ribozyme (2002)
Sood, Vanita D., Yekta, Soraya, Collins, Richard A.
We have used nucleotide analog interference mapping and site-specific substitution to determine the effect of 2′-deoxynucleotide substitution of each nucleotide in the VS ribozyme on the...
Sojourners [Mikroform] : christians in the world in the theology of Hans Urs von Balthasar / (1997)
Diss. phil. Maryland University, College Park, 1996.
Thesis (Ph. D.)--Marquette University, 1996.
Ligand-induced conformational changes in wild-type and mutant yeast pyruvate kinase (1996)
Collins, Richard A., Kelly, Sharon M., Price, Nicholas C., Fothergill-Gilmore, Linda A., Muirhead, Hilary
A mutant form of pyruvate kinase in which serine 384 has been mutated to proline has been engineered in the yeast Saccharomyces cerevisiae. Residue 384 is located in a helix in a subunit interface of...
Field, Deborah J., Sommerfield, Adele, Saville, Barry J., Collins, Richard A.
The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in...
Collins, Richard A., Reynolds, Catherine A., Olive, Joan
We have determined the DNA sequence of intron 1 and flanking exons in the mitochondrial apocytochrome b gene of the Neurospora laboratory strain 74 and the natural isolate North Africa. In contrast...
Stohl, Lori L., Collins, Richard A., Cole, Michael D., Lambowitz, Alan M.
Mitochondria from two Neurospora intermedia strains (P405-Labelle and Fiji N6-6) were found to contain plasmid DNAs in addition to the standard mitochondrial DNA species. The plasmid DNAs consist of...
Andersen, Angela A., Collins, Richard A.
Kissing interactions in RNA are formed when bases between two hairpin loops pair. Intra- and intermolecular kissing interactions are important in forming the tertiary or quaternary structure of many...
The contribution of 2′-hydroxyls to the cleavage activity of the Neurospora VS ribozyme
Sood, Vanita D., Yekta, Soraya, Collins, Richard A.
We have used nucleotide analog interference mapping and site-specific substitution to determine the effect of 2′-deoxynucleotide substitution of each nucleotide in the VS ribozyme on the...
Rapid formation of a solvent-inaccessible core in the Neurospora Varkud satellite ribozyme
Hiley, Shawna L., Collins, Richard A.
We have used hydroxyl radicals generated by decomposition of peroxynitrous acid to study Mg2+-dependent structure and folding of the Varkud satellite (VS) ribozyme. Protection from radical cleavage...
4-thio-U cross-linking identifies the active site of the VS ribozyme
Hiley, Shawna L., Sood, Vanita D., Fan, Jennie, Collins, Richard A.
To identify nucleotides in or near the active site, we have used a circularly permuted version of the VS ribozyme capable of cleavage and ligation to incorporate a single photoactive nucleotide...
NMR structure of the active conformation of the Varkud satellite ribozyme cleavage site
Hoffmann, Bernd, Mitchell, G. Thomas, Gendron, Patrick, Major, François, Andersen, Angela A., Collins, Richard A., ...
Substrate cleavage by the Neurospora Varkud satellite (VS) ribozyme involves a structural change in the stem-loop I substrate from an inactive to an active conformation. We have determined the NMR...
Exceptionally fast self-cleavage by a Neurospora Varkud satellite ribozyme
Zamel, Ricardo, Poon, Alan, Jaikaran, Dominic, Andersen, Angela, Olive, Joan, De Abreu, Diane, ...
Most of the small ribozymes, including those that have been investigated as potential therapeutic agents, appear to be rather poor catalysts. These RNAs use an internal phosphoester transfer...
Andersen, Angela A., Collins, Richard A.
Kissing interactions in RNA are formed when bases between two hairpin loops pair. Intra- and intermolecular kissing interactions are important in forming the tertiary or quaternary structure of many...
The contribution of 2′-hydroxyls to the cleavage activity of the Neurospora VS ribozyme
Sood, Vanita D., Yekta, Soraya, Collins, Richard A.
We have used nucleotide analog interference mapping and site-specific substitution to determine the effect of 2′-deoxynucleotide substitution of each nucleotide in the VS ribozyme on the...
Rapid formation of a solvent-inaccessible core in the Neurospora Varkud satellite ribozyme
Hiley, Shawna L., Collins, Richard A.
We have used hydroxyl radicals generated by decomposition of peroxynitrous acid to study Mg2+-dependent structure and folding of the Varkud satellite (VS) ribozyme. Protection from radical cleavage...
4-thio-U cross-linking identifies the active site of the VS ribozyme
Hiley, Shawna L., Sood, Vanita D., Fan, Jennie, Collins, Richard A.
To identify nucleotides in or near the active site, we have used a circularly permuted version of the VS ribozyme capable of cleavage and ligation to incorporate a single photoactive nucleotide...
NMR structure of the active conformation of the Varkud satellite ribozyme cleavage site
Hoffmann, Bernd, Mitchell, G. Thomas, Gendron, Patrick, Major, François, Andersen, Angela A., Collins, Richard A., ...
Substrate cleavage by the Neurospora Varkud satellite (VS) ribozyme involves a structural change in the stem-loop I substrate from an inactive to an active conformation. We have determined the NMR...
Exceptionally fast self-cleavage by a Neurospora Varkud satellite ribozyme
Zamel, Ricardo, Poon, Alan, Jaikaran, Dominic, Andersen, Angela, Olive, Joan, De Abreu, Diane, ...
Most of the small ribozymes, including those that have been investigated as potential therapeutic agents, appear to be rather poor catalysts. These RNAs use an internal phosphoester transfer...
Evidence for proton transfer in the rate-limiting step of a fast-cleaving Varkud satellite ribozyme
Smith, M. Duane, Collins, Richard A.
A fast-cleaving version of the Varkud satellite ribozyme, called RG, shows an apparent cis-cleavage rate constant of 5 sec−1, similar to the rates of protein enzymes that catalyze similar...
Jaikaran, Dominic, Smith, M. Duane, Mehdizadeh, Reza, Olive, Joan, Collins, Richard A.
We describe a chemical coupling procedure that allows joining of two RNAs, one of which contains a site-specific base analog substitution, in the absence of divalent ions. This method allows...