Richard Calendar

The terminally redundant, nonpermuted genome of Listeria bacteriophage A511: a model for the SPO1-like myoviruses of gram-positive bacteria (2008)

Klumpp, Jochen, Dorscht, Julia, Lurz, Rudi, Bielmann, Regula, Wieland, Matthias, Zimmer, Markus, ...

Only little information on a particular class of myoviruses, the SPO1-like bacteriophages infecting low-G+C-content, gram-positive host bacteria (Firmicutes), is available. We present the genome...

Phage P4 origin-binding domain structure reveals a mechanism for regulation of DNA-binding activity by homo- and heterodimerization of winged helix proteins (2002)

Yeo,Hye-Jeong, Ziegelin,Günter, Korolev,Sergey, Calendar,Richard, Lanka,Erich, Waksman,Gabriel

The origin-binding domain of the gp protein of phage P4 (P4-OBD) mediates origin recognition and regulation of gp activity by the protein Cnr. We have determined the crystal structure of P4-OBD at...

Phage P4 origin-binding domain structure reveals a mechanism for regulation of DNA-binding activity by homo- and heterodimerization of winged helix proteins (2002)

Yeo, Hye-Jeong, Ziegelin, Günter, Korolev, Sergey, Calendar, Richard, Lanka, Erich, Waksman, Gabriel

The origin-binding domain of the gp protein of phage P4 (P4-OBD) mediates origin recognition and regulation of gp activity by the protein Cnr. We have determined the crystal structure of P4-OBD at...

Nucleotide sequence of the DNA packaging and capsid synthesis geges of bacteriophage P2 (1991)

Linderoth, Nora A., Ziermann, Rainer, Haggård-Ljungquist, Elisabeth, Christie, Gail E., Calendar, Richard

Overlapping DMA fragments containing the DNA packaging and capsid synthesis gene region of bacterlophage P2 were cloned and sequenced. In this report we present the complete nucleotide sequence of...

The P2 phage old gene: sequence, transcription and translational control (1989)

Haggard-Ljungquist, Elisabeth, Barreiro, Virginia, Calendar, Richard, Kurnit, David M., Hans Cheng,

The old (overcoming lysogenization defect) gene product of bacteriophage P2 kills Escherichia coli recB and recC mutants and interferes with phage [lambda] growth [ Sironi et al., Virology 46 (1971)...

Novel topologically knotted DNA from bacteriophage P4 capsids: studies with DNA topoisomerases (1981)

Liu, Leroy F., Davis, John L., Calendar, Richard

DNA molecules isolated from bacteriophage P4 are mostly linear with cohesive ends capable of forming cicular and concatemeric structures. In contrast, almost all DNA molecules isolated from P4...

Tyrosyl RNA synthesis. (1966)

Calendar, Richard.

Thesis (Ph. D.)--Dept. of Biochemistry, Stanford University, 1967.

A σ32 mutant with a single amino acid change in the highly conserved region 2.2 exhibits reduced core RNA polymerase affinity

Joo, Daniel M., Ng, Norman, Calendar, Richard

σ32, the product of the rpoH gene in Escherichia coli, provides promoter specificity by interacting with core RNAP. Amino acid sequence alignment of σ32 with other sigma factors in the σ70 family...

Multiple Regions on the Escherichia coli Heat Shock Transcription Factor ς32 Determine Core RNA Polymerase Binding Specificity

Joo, Daniel M., Nolte, Audrey, Calendar, Richard, Zhou, Yan Ning, Jin, Ding Jun

We have analyzed the core RNA polymerase (RNAP) binding activity of the purified products of nine defective alleles of the rpoH gene, which encodes ς32 in Escherichia coli. All mutations studied...

A Complex Control System for Transcriptional Activation from the sid Promoter of Bacteriophage P4

Reiter, Kaye, Lam, Hugh, Young, Edward, Julien, Bryan, Calendar, Richard

The sid gene promoter (Psid), which controls expression of the late genes from satellite phage P4, is activated by a unique class of small DNA-binding proteins. The activators from both satellite and...

An Upstream Sequence Element Required for NucC-Dependent Expression of the Serratia marcescens Extracellular Nuclease

Winslow, Robert H., Julien, Bryan, Calendar, Richard, Christie, Gail E.

The Serratia marcescens extracellular nuclease gene, nucA, is positively regulated by the product of the nucC gene. In this study, the upstream region required for NucC-dependent nuclease expression...

Construction, Characterization, and Use of Two Listeria monocytogenes Site-Specific Phage Integration Vectors

Lauer, Peter, Chow, Man Yin Nora, Loessner, Martin J., Portnoy, Daniel A., Calendar, Richard

Two site-specific shuttle integration vectors were developed with two different chromosomal bacteriophage integration sites to facilitate strain construction in Listeria monocytogenes. The first...

Identification of Upstream Sequences Essential for Activation of a Bacteriophage P2 Late Promoter

Christie, Gail E., Anders, Douglas L., McAlister, Victor, Goodwin, Tina S., Julien, Bryan, Calendar, Richard

We have carried out a mutational scan of the upstream region of the bacteriophage P2 FETUD late operon promoter, PF, which spans an element of hyphenated dyad symmetry that is conserved among all six...

Bacteriophage Lambda; Abortive Infection of Bacteria Lysogenic for Phage P2*

Lindahl, Gunnar, Sironi, Gianpiero, Bialy, Harvey, Calendar, Richard

The efficiency of plating of wild-type λ on a host lysogenic for P2 is less than 10-6, and only a small number of infected cells produce progeny phage. Lambda can adsorb and inject its DNA normally...

In Vitro Packaging of Satellite Phage P4 DNA

Pruss, Gail, Goldstein, Richard N., Calendar, Richard

Satellite phage P4 directs the capsid proteins of its helper phage, P2, to form a head which is only one-third the size of the normal P2 head. The P2 head contains a genome of molecular weight 22 ×...

A Transcribing Activity Induced by Satellite Phage P4

Barrett, Kathleen J., Gibbs, Warren, Calendar, Richard

Satellite bacteriophage P4 induces a new transcribing enzyme that synthesizes polyriboguanylic acid in the presence of the poly(dG)·poly(dC) homopolymer pair. This transcribing activity was...

P2 Growth Restriction on an rpoC Mutant Is Suppressed by Alleles of the Rz1 Homolog lysC

Markov, Dmitry, Christie, Gail E., Sauer, Brian, Calendar, Richard, Park, Taehyun, Young, Ry, ...

Escherichia coli strain 397c carries a temperature-sensitive mutation, rpoC397, that removes the last 50 amino acids of the RNA polymerase β′ subunit and is nonpermissive for plating of...

A σ32 mutant with a single amino acid change in the highly conserved region 2.2 exhibits reduced core RNA polymerase affinity

Joo, Daniel M., Ng, Norman, Calendar, Richard

σ32, the product of the rpoH gene in Escherichia coli, provides promoter specificity by interacting with core RNAP. Amino acid sequence alignment of σ32 with other sigma factors in the σ70 family...

Multiple Regions on the Escherichia coli Heat Shock Transcription Factor ς32 Determine Core RNA Polymerase Binding Specificity

Joo, Daniel M., Nolte, Audrey, Calendar, Richard, Zhou, Yan Ning, Jin, Ding Jun

We have analyzed the core RNA polymerase (RNAP) binding activity of the purified products of nine defective alleles of the rpoH gene, which encodes ς32 in Escherichia coli. All mutations studied...

A Complex Control System for Transcriptional Activation from the sid Promoter of Bacteriophage P4

Reiter, Kaye, Lam, Hugh, Young, Edward, Julien, Bryan, Calendar, Richard

The sid gene promoter (Psid), which controls expression of the late genes from satellite phage P4, is activated by a unique class of small DNA-binding proteins. The activators from both satellite and...

An Upstream Sequence Element Required for NucC-Dependent Expression of the Serratia marcescens Extracellular Nuclease

Winslow, Robert H., Julien, Bryan, Calendar, Richard, Christie, Gail E.

The Serratia marcescens extracellular nuclease gene, nucA, is positively regulated by the product of the nucC gene. In this study, the upstream region required for NucC-dependent nuclease expression...

Construction, Characterization, and Use of Two Listeria monocytogenes Site-Specific Phage Integration Vectors

Lauer, Peter, Chow, Man Yin Nora, Loessner, Martin J., Portnoy, Daniel A., Calendar, Richard

Two site-specific shuttle integration vectors were developed with two different chromosomal bacteriophage integration sites to facilitate strain construction in Listeria monocytogenes. The first...

Identification of Upstream Sequences Essential for Activation of a Bacteriophage P2 Late Promoter

Christie, Gail E., Anders, Douglas L., McAlister, Victor, Goodwin, Tina S., Julien, Bryan, Calendar, Richard

We have carried out a mutational scan of the upstream region of the bacteriophage P2 FETUD late operon promoter, PF, which spans an element of hyphenated dyad symmetry that is conserved among all six...

Bacteriophage Lambda; Abortive Infection of Bacteria Lysogenic for Phage P2*

Lindahl, Gunnar, Sironi, Gianpiero, Bialy, Harvey, Calendar, Richard

The efficiency of plating of wild-type λ on a host lysogenic for P2 is less than 10-6, and only a small number of infected cells produce progeny phage. Lambda can adsorb and inject its DNA normally...

In Vitro Packaging of Satellite Phage P4 DNA

Pruss, Gail, Goldstein, Richard N., Calendar, Richard

Satellite phage P4 directs the capsid proteins of its helper phage, P2, to form a head which is only one-third the size of the normal P2 head. The P2 head contains a genome of molecular weight 22 ×...

A Transcribing Activity Induced by Satellite Phage P4

Barrett, Kathleen J., Gibbs, Warren, Calendar, Richard

Satellite bacteriophage P4 induces a new transcribing enzyme that synthesizes polyriboguanylic acid in the presence of the poly(dG)·poly(dC) homopolymer pair. This transcribing activity was...

P2 Growth Restriction on an rpoC Mutant Is Suppressed by Alleles of the Rz1 Homolog lysC

Markov, Dmitry, Christie, Gail E., Sauer, Brian, Calendar, Richard, Park, Taehyun, Young, Ry, ...

Escherichia coli strain 397c carries a temperature-sensitive mutation, rpoC397, that removes the last 50 amino acids of the RNA polymerase β′ subunit and is nonpermissive for plating of...

The Terminally Redundant, Nonpermuted Genome of Listeria Bacteriophage A511: a Model for the SPO1-Like Myoviruses of Gram-Positive Bacteria▿ †

Klumpp, Jochen, Dorscht, Julia, Lurz, Rudi, Bielmann, Regula, Wieland, Matthias, Zimmer, Markus, ...

Only little information on a particular class of myoviruses, the SPO1-like bacteriophages infecting low-G+C-content, gram-positive host bacteria (Firmicutes), is available. We present the genome...

Stable Integration Vector for Nutrient Broth-Based Selection of Attenuated Listeria monocytogenes Strains with Recombinant Antigen Expression▿

Lenz, Laurel L., Huang, William A., Zhou, Chenghui, Li, Zhongxia, Calendar, Richard

Recombinant Listeria monocytogenes strains induce strong cellular immune responses and may prove useful for antigen delivery for the vaccination of humans. However, the genetic systems currently...

Killed but Metabolically Active Bacillus anthracis Vaccines Induce Broad and Protective Immunity against Anthrax▿

Skoble, Justin, Beaber, John W., Gao, Yi, Lovchik, Julie A., Sower, Laurie E., Liu, Weiqun, ...

Bacillus anthracis is the causative agent of anthrax. We have developed a novel whole-bacterial-cell anthrax vaccine utilizing B. anthracis that is killed but metabolically active (KBMA). Vaccine...