Spirochete-platelet attachment and thrombocytopenia in murine relapsing fever borreliosis (2003)
Alugupalli, Kishore R., Michelson, Alan D., Joris, Isabelle, Schwan, Tom G., Hodivala-Dilke, Kairbaan, Hynes, Richard O., ...
Thrombocytopenia is common in persons infected with relapsing fever Borreliae. We previously showed that the relapsing fever spirochete Borrelia hermsii binds to and activates human platelets in...
Alcamo, Elizabeth, Hacohen, Nir, Schulte, Leah C., Rennert, Paul D., Hynes, Richard O., Baltimore, David
The transcription factor nuclear factor (NF)-kappaB has been suggested to be a key mediator of the development of lymph nodes and Peyer's patches. However, targeted deletion of NF-kappaB/ Rel family...
In vitro splicing of fibronectin pre-mRNAs (1990)
Norton, Pamela A., Hynes, Richard O.
We have investigated the alternative splicing of the EIIIB exon of the rat fibronectin gene. Mini-gene constructs containing this exon and portions of adjacent introns and exons, when transfected...
Surfaces of normal and malignant cells / edited by Richard O. Hynes (1979)
Incluye bibliografía e índice
Thesis (Ph. D.)--Massachusetts Institute of Technology, 1971.
Saoncella, Stefania, Echtermeyer, Frank, Denhez, Fabienne, Nowlen, Julie K., Mosher, Deane F., Robinson, Stephen D., ...
The assembly of focal adhesions and actin stress fibers by cells plated on fibronectin depends on adhesion-mediated signals involving both integrins and cell-surface heparan sulfate proteoglycans....
Robinson, Stephen D., Frenette, Paul S., Rayburn, Helen, Cummiskey, Marge, Ullman-Culleré, Mollie, Wagner, Denisa D., ...
We extend our previous analyses of mice deficient in selectins by describing the generation and comparative phenotype of mice lacking one, two, or three selectins after sequential ablation of the...
A mouse model of severe von Willebrand disease: Defects in hemostasis and thrombosis
Denis, Cécile, Methia, Nassia, Frenette, Paul S., Rayburn, Helen, Ullman-Culleré, Mollie, Hynes, Richard O., ...
von Willebrand factor (vWf) deficiency causes severe von Willebrand disease in humans. We generated a mouse model for this disease by using gene targeting. vWf-deficient mice appeared normal at...
Fox, George L., Rebay, Ilaria, Hynes, Richard O.
Focal adhesion kinase (FAK) is a highly conserved, cytoplasmic tyrosine kinase that has been implicated in promoting cell migration and transmission of antiapoptotic signals in vertebrate cells. In...
Control of alternative pre-mRNA splicing by distributed pentameric repeats
Hedjran, Farah, Yeakley, Joanne M., Huh, Gene S., Hynes, Richard O., Rosenfeld, Michael G.
Multiple copies of the hexamer TGCATG have been shown to regulate fibronectin pre-mRNA alternative splicing. GCATG repeats also are clustered near the regulated calcitonin-specific 3′ splice site...
Bloom, Laird, Ingham, Kenneth C., Hynes, Richard O.
Fibroblasts, when plated on the extracellular matrix protein fibronectin (FN), rapidly spread and form an organized actin cytoskeleton. This process is known to involve both the central α5β1...
Layilin, a Novel Integral Membrane Protein, Is a Hyaluronan Receptor
Bono, Petri, Rubin, Kristofer, Higgins, Jonathan M. G., Hynes, Richard O.
The actin cytoskeleton plays a significant role in changes of cell shape and motility, and interactions between the actin filaments and the cell membrane are crucial for a variety of cellular...
Rodríguez-Manzaneque, Juan Carlos, Lane, Timothy F., Ortega, María Asunción, Hynes, Richard O., Lawler, Jack, Iruela-Arispe, M. Luisa
Growth of tumors and metastasis are processes known to require neovascularization. To ascertain the participation of the endogenous angiogenic inhibitor thrombospondin-1 (TSP1) in tumor progression,...
E and P Selectins Are Not Required for Resistance to Severe Murine Lyme Arthritis
Seiler, Kathleen Petri, Ma, Ying, Weis, John H., Frenette, Paul S., Hynes, Richard O., Wagner, Denisa D., ...
Borrelia burgdorferi-induced arthritis in mice is characterized by tendonitis, synovitis, and inflammatory-cell infiltrate, predominantly of neutrophils. Because genetic deficiency in E and P...
Borsig, Lubor, Wong, Richard, Hynes, Richard O., Varki, Nissi M., Varki, Ajit
P-selectin facilitates human carcinoma metastasis in immunodeficient mice by mediating early interactions of platelets with bloodborne tumor cells via their cell surface mucins, and this process can...
Whittaker, Charles A., Hynes, Richard O.
The von Willebrand A (VWA) domain is a well-studied domain involved in cell adhesion, in extracellular matrix proteins, and in integrin receptors. A number of human diseases arise from mutations in...
McCarty, Joseph H., Monahan-Earley, Rita A., Brown, Lawrence F., Keller, Markus, Gerhardt, Holger, Rubin, Kristofer, ...
Mouse embryos genetically null for the αv integrin subunit develop intracerebral hemorrhages at midgestation and die shortly after birth. A key question is whether the hemorrhage arises from primary...
Plasma fibronectin promotes thrombus growth and stability in injured arterioles
Ni, Heyu, Yuen, Peter S. T., Papalia, Jessie M., Trevithick, Jane E., Sakai, Takao, Fässler, Reinhard, ...
Mice lacking both of the best-known platelet ligands, von Willebrand factor and fibrinogen, can still form occlusive thrombi in injured arterioles. The platelets of these animals accumulate excessive...
Mice lacking β3 integrins are osteosclerotic because of dysfunctional osteoclasts
McHugh, Kevin P., Hodivala-Dilke, Kairbaan, Zheng, Ming-Hao, Namba, Noriyuki, Lam, Jonathan, Novack, Deborah, ...
Osteoclasts express the αvβ3 integrin, an adhesion receptor that has been implicated in bone resorption and that is therefore a potential therapeutic target. To assess the role of this heterodimer...
Ni, Heyu, Denis, Cécile V., Subbarao, Sangeetha, Degen, Jay L., Sato, Thomas N., Hynes, Richard O., ...
We used intravital microscopy to observe the formation of platelet plugs in ferric chloride–injured arterioles of live mice. With this model, we evaluated thrombus growth in mice lacking von...
Increased primary tumor growth in mice null for β3- or β3/β5-integrins or selectins
Taverna, Daniela, Moher, Heather, Crowley, Denise, Borsig, Lubor, Varki, Ajit, Hynes, Richard O.
Expression of αvβ3- or αvβ5-integrins and selectins is widespread on blood cells and endothelial cells. Here we report that human tumor cells injected s.c. into mice lacking β3- or...
Hodivala-Dilke, Kairbaan M., McHugh, Kevin P., Tsakiris, Dimitrios A., Rayburn, Helen, Crowley, Denise, Ullman-Culleré, Mollie, ...
β3 integrins have been implicated in a wide variety of functions, including platelet aggregation and thrombosis (αIIbβ3) and implantation, placentation, angiogenesis, bone remodeling, and tumor...
Alteration of Cell-Surface Proteins by Viral Transformation and by Proteolysis
Putative cell-surface proteins of tissue-culture cells were identified by lactoperoxidase-catalyzed iodination, a technique that attaches label only to proteins outside the cell membrane. Evidence is...
Astrof, Sophie, Crowley, Denise, George, Elizabeth L., Fukuda, Tomohiko, Sekiguchi, Kiyotoshi, Hanahan, Douglas, ...
Fibronectin splice variants containing the EIIIA and/or EIIIB exons are prominently expressed in the vasculature of a variety of human tumors but not in normal adult tissues. To understand the...
McCarty, Joseph H., Cook, Aaron A., Hynes, Richard O.
αvβ8 integrin is highly expressed in the vertebrate CNS, and mice lacking the αv or β8 genes develop cerebral hemorrhage due to defective interactions between blood vessels and αvβ8-expressing...
Lymphatic or Hematogenous Dissemination: How Does a Metastatic Tumor Cell Decide?
Wong, Sunny Y., Hynes, Richard O.
The formation of distant metastases is the deadliest phase of cancer progression. Although numerous studies have identified genes and mechanisms that affect metastasis after tumors have reached...
Xu, Lei, Begum, Shahinoor, Hearn, Jeremy D., Hynes, Richard O.
The survival and growth of tumor cells in a foreign environment is considered a rate-limiting step during metastasis. To identify genes that may be essential for this process, we isolated highly...
Saoncella, Stefania, Echtermeyer, Frank, Denhez, Fabienne, Nowlen, Julie K., Mosher, Deane F., Robinson, Stephen D., ...
The assembly of focal adhesions and actin stress fibers by cells plated on fibronectin depends on adhesion-mediated signals involving both integrins and cell-surface heparan sulfate proteoglycans....
Robinson, Stephen D., Frenette, Paul S., Rayburn, Helen, Cummiskey, Marge, Ullman-Culleré, Mollie, Wagner, Denisa D., ...
We extend our previous analyses of mice deficient in selectins by describing the generation and comparative phenotype of mice lacking one, two, or three selectins after sequential ablation of the...
A mouse model of severe von Willebrand disease: Defects in hemostasis and thrombosis
Denis, Cécile, Methia, Nassia, Frenette, Paul S., Rayburn, Helen, Ullman-Culleré, Mollie, Hynes, Richard O., ...
von Willebrand factor (vWf) deficiency causes severe von Willebrand disease in humans. We generated a mouse model for this disease by using gene targeting. vWf-deficient mice appeared normal at...
Fox, George L., Rebay, Ilaria, Hynes, Richard O.
Focal adhesion kinase (FAK) is a highly conserved, cytoplasmic tyrosine kinase that has been implicated in promoting cell migration and transmission of antiapoptotic signals in vertebrate cells. In...
Control of alternative pre-mRNA splicing by distributed pentameric repeats
Hedjran, Farah, Yeakley, Joanne M., Huh, Gene S., Hynes, Richard O., Rosenfeld, Michael G.
Multiple copies of the hexamer TGCATG have been shown to regulate fibronectin pre-mRNA alternative splicing. GCATG repeats also are clustered near the regulated calcitonin-specific 3′ splice site...
Bloom, Laird, Ingham, Kenneth C., Hynes, Richard O.
Fibroblasts, when plated on the extracellular matrix protein fibronectin (FN), rapidly spread and form an organized actin cytoskeleton. This process is known to involve both the central α5β1...
Layilin, a Novel Integral Membrane Protein, Is a Hyaluronan Receptor
Bono, Petri, Rubin, Kristofer, Higgins, Jonathan M. G., Hynes, Richard O.
The actin cytoskeleton plays a significant role in changes of cell shape and motility, and interactions between the actin filaments and the cell membrane are crucial for a variety of cellular...
Rodríguez-Manzaneque, Juan Carlos, Lane, Timothy F., Ortega, María Asunción, Hynes, Richard O., Lawler, Jack, Iruela-Arispe, M. Luisa
Growth of tumors and metastasis are processes known to require neovascularization. To ascertain the participation of the endogenous angiogenic inhibitor thrombospondin-1 (TSP1) in tumor progression,...
E and P Selectins Are Not Required for Resistance to Severe Murine Lyme Arthritis
Seiler, Kathleen Petri, Ma, Ying, Weis, John H., Frenette, Paul S., Hynes, Richard O., Wagner, Denisa D., ...
Borrelia burgdorferi-induced arthritis in mice is characterized by tendonitis, synovitis, and inflammatory-cell infiltrate, predominantly of neutrophils. Because genetic deficiency in E and P...
Borsig, Lubor, Wong, Richard, Hynes, Richard O., Varki, Nissi M., Varki, Ajit
P-selectin facilitates human carcinoma metastasis in immunodeficient mice by mediating early interactions of platelets with bloodborne tumor cells via their cell surface mucins, and this process can...
Whittaker, Charles A., Hynes, Richard O.
The von Willebrand A (VWA) domain is a well-studied domain involved in cell adhesion, in extracellular matrix proteins, and in integrin receptors. A number of human diseases arise from mutations in...
McCarty, Joseph H., Monahan-Earley, Rita A., Brown, Lawrence F., Keller, Markus, Gerhardt, Holger, Rubin, Kristofer, ...
Mouse embryos genetically null for the αv integrin subunit develop intracerebral hemorrhages at midgestation and die shortly after birth. A key question is whether the hemorrhage arises from primary...
Plasma fibronectin promotes thrombus growth and stability in injured arterioles
Ni, Heyu, Yuen, Peter S. T., Papalia, Jessie M., Trevithick, Jane E., Sakai, Takao, Fässler, Reinhard, ...
Mice lacking both of the best-known platelet ligands, von Willebrand factor and fibrinogen, can still form occlusive thrombi in injured arterioles. The platelets of these animals accumulate excessive...
Mice lacking β3 integrins are osteosclerotic because of dysfunctional osteoclasts
McHugh, Kevin P., Hodivala-Dilke, Kairbaan, Zheng, Ming-Hao, Namba, Noriyuki, Lam, Jonathan, Novack, Deborah, ...
Osteoclasts express the αvβ3 integrin, an adhesion receptor that has been implicated in bone resorption and that is therefore a potential therapeutic target. To assess the role of this heterodimer...
Ni, Heyu, Denis, Cécile V., Subbarao, Sangeetha, Degen, Jay L., Sato, Thomas N., Hynes, Richard O., ...
We used intravital microscopy to observe the formation of platelet plugs in ferric chloride–injured arterioles of live mice. With this model, we evaluated thrombus growth in mice lacking von...
Increased primary tumor growth in mice null for β3- or β3/β5-integrins or selectins
Taverna, Daniela, Moher, Heather, Crowley, Denise, Borsig, Lubor, Varki, Ajit, Hynes, Richard O.
Expression of αvβ3- or αvβ5-integrins and selectins is widespread on blood cells and endothelial cells. Here we report that human tumor cells injected s.c. into mice lacking β3- or...
Hodivala-Dilke, Kairbaan M., McHugh, Kevin P., Tsakiris, Dimitrios A., Rayburn, Helen, Crowley, Denise, Ullman-Culleré, Mollie, ...
β3 integrins have been implicated in a wide variety of functions, including platelet aggregation and thrombosis (αIIbβ3) and implantation, placentation, angiogenesis, bone remodeling, and tumor...
Alteration of Cell-Surface Proteins by Viral Transformation and by Proteolysis
Putative cell-surface proteins of tissue-culture cells were identified by lactoperoxidase-catalyzed iodination, a technique that attaches label only to proteins outside the cell membrane. Evidence is...
Astrof, Sophie, Crowley, Denise, George, Elizabeth L., Fukuda, Tomohiko, Sekiguchi, Kiyotoshi, Hanahan, Douglas, ...
Fibronectin splice variants containing the EIIIA and/or EIIIB exons are prominently expressed in the vasculature of a variety of human tumors but not in normal adult tissues. To understand the...
McCarty, Joseph H., Cook, Aaron A., Hynes, Richard O.
αvβ8 integrin is highly expressed in the vertebrate CNS, and mice lacking the αv or β8 genes develop cerebral hemorrhage due to defective interactions between blood vessels and αvβ8-expressing...
Xu, Lei, Begum, Shahinoor, Hearn, Jeremy D., Hynes, Richard O.
The survival and growth of tumor cells in a foreign environment is considered a rate-limiting step during metastasis. To identify genes that may be essential for this process, we isolated highly...
Thrombospondin-1 Gene Expression Affects Survival and Tumor Spectrum of p53-Deficient Mice
Lawler, Jack, Miao, Wei-Min, Duquette, Mark, Bouck, Noël, Bronson, Roderick T., Hynes, Richard O.
In vitro and in vivo data indicate that thrombospondin-1 (TSP1) inhibits tumor progression in several ways including direct effects on cellular growth and apoptosis in the stromal compartment. To...
Fang, Juan, Hodivala-Dilke, Kairbaan, Johnson, Bryon D., Du, Lily M., Hynes, Richard O., White, Gilbert C., ...
Integrins mediate the adhesion of cells to each other and to the extracellular matrix during development, immunity, metastasis, thrombosis, and wound healing. Molecular defects in either the α- or...
Ni, Heyu, Chen, Pingguo, Spring, Christopher M., Sayeh, Ebrahim, Semple, John W., Lazarus, Alan H., ...
Fetal and neonatal alloimmune thrombo cytopenia (FNAITP) is a life-threatening bleeding disorder caused by maternal antibodies directed against fetal platelet antigens. The immunoreactive epitopes in...
Eshghi, Shawdee, Vogelezang, Mariette G., Hynes, Richard O., Griffith, Linda G., Lodish, Harvey F.
Erythropoietin (Epo) is essential for the terminal proliferation and differentiation of erythroid progenitor cells. Fibronectin is an important part of the erythroid niche, but its precise role in...
Protein 4.1B suppresses prostate cancer progression and metastasis
Wong, Sunny Y., Haack, Herbert, Kissil, Joseph L., Barry, Marc, Bronson, Roderick T., Shen, Steven S., ...
Protein 4.1B is a 4.1/ezrin/radixin/moesin domain-containing protein whose expression is frequently lost in a variety of human tumors, including meningiomas, non-small-cell lung cancers, and breast...
Ulcerative colitis and autoimmunity induced by loss of myeloid αv integrins
Lacy-Hulbert, Adam, Smith, Aileen M., Tissire, Hamid, Barry, Marc, Crowley, Denise, Bronson, Roderick T., ...
The gastrointestinal tract is constantly challenged by foreign antigens and commensal bacteria but nonetheless is able to maintain a state of immunological quiescence. Recent advances have...
Interaction of Dendritic Cells with Skin Endothelium: A New Perspective on Immunosurveillance
Robert, Caroline, Fuhlbrigge, Robert C., Kieffer, J. David, Ayehunie, Seyoum, Hynes, Richard O., Cheng, Guiying, ...
The goal of this study was to determine the mechanisms by which dendritic cells (DCs) in blood could interact with endothelium, a prerequisite to extravasation into tissues. Our results indicate that...
Requirement for the NF-κB Family Member RelA in the Development of Secondary Lymphoid Organs
Alcamo, Elizabeth, Hacohen, Nir, Schulte, Leah C., Rennert, Paul D., Hynes, Richard O., Baltimore, David
The transcription factor nuclear factor (NF)-κB has been suggested to be a key mediator of the development of lymph nodes and Peyer's patches. However, targeted deletion of NF-κB/ Rel family...
Mazo, Irina B., Gutierrez-Ramos, Jose-Carlos, Frenette, Paul S., Hynes, Richard O., Wagner, Denisa D., Von Andrian, Ulrich H.
We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions...
CHARACTERIZATION OF THE EXTERNAL PROTEINS OF HAMSTER FIBROBLASTS
Hynes, Richard O., Humphryes, Kenneth C.
The results of metabolic labeling studies and enzymatic treatments followed by analysis on polyacrylamide gels show that the external proteins of hamster fibroblast cell lines, which have been...
Borowsky, Mark L., Hynes, Richard O.
Changes in cell morphology and motility are mediated by the actin cytoskeleton. Recent advances in our understanding of the regulators of microfilament structure and dynamics have shed light on how...
Hartwell, Daqing W., Mayadas, Tanya N., Berger, Gaëtan, Frenette, Paul S., Rayburn, Helen, Hynes, Richard O., ...
P-selectin is an adhesion receptor for leukocytes expressed on activated platelets and endothelial cells. The cytoplasmic domain of P-selectin was shown in vitro to contain signals required for both...
Integrin-mediated Signals Regulated by Members of the Rho Family of GTPases
Clark, Edwin A., King, Warren G., Brugge, Joan S., Symons, Marc, Hynes, Richard O.
The organization of the actin cytoskeleton can be regulated by soluble factors that trigger signal transduction events involving the Rho family of GTPases. Since adhesive interactions are also...
Fusion Competence of Myoblasts Rendered Genetically Null for N-Cadherin in Culture
Charlton, Carol A., Mohler, William A., Radice, Glenn L., Hynes, Richard O., Blau, Helen M.
Myoblast fusion is essential to muscle tissue development yet remains poorly understood. N-cadherin, like other cell surface adhesion molecules, has been implicated by others in muscle formation...
α3β1 Integrin Is Required for Normal Development of the Epidermal Basement Membrane
DiPersio, C. Michael, Hodivala-Dilke, Kairbaan M., Jaenisch, Rudolf, Kreidberg, Jordan A., Hynes, Richard O.
Integrins α3β1 and α6β4 are abundant receptors on keratinocytes for laminin-5, a major component of the basement membrane between the epidermis and the dermis in skin. These integrins are...
Precocious Mammary Gland Development in P-Cadherin–deficient Mice
Radice, Glenn L., Ferreira-Cornwell, M. Celeste, Robinson, Stephen D., Rayburn, Helen, Chodosh, Lewis A., Takeichi, Masatoshi, ...
To investigate the functions of P-cadherin in vivo, we have mutated the gene encoding this cell adhesion receptor in mice. In contrast to E- and N-cadherin– deficient mice, mice homozygous for the...
Dystrophic Muscle in Mice Chimeric for Expression of α5 Integrin
Taverna, Daniela, Disatnik, Marie-Helene, Rayburn, Helen, Bronson, Roderick T., Yang, Joy, Rando, Thomas A., ...
α5-deficient mice die early in embryogenesis (Yang et al., 1993). To study the functions of α5 integrin later in mouse embryogenesis and during adult life we generated α5 −/−;+/+ chimeric...
Hodivala-Dilke, Kairbaan M., Michael DiPersio, C., Kreidberg, Jordan A., Hynes, Richard O.
Previously we found that α3β1 integrin–deficient neonatal mice develop micro-blisters at the epidermal–dermal junction. These micro-blisters were associated with poor basement membrane...
McCarty, Joseph H., Barry, Marc, Crowley, Denise, Bronson, Roderick T., Lacy-Hulbert, Adam, Hynes, Richard O.
Integrin-mediated cell adhesion and signaling events are essential for the proper development and homeostasis of most epithelial tissues. Dysregulation of integrin expression and function can cause...
A system for Cre-regulated RNA interference in vivo
Stern, Patrick, Astrof, Sophie, Erkeland, Stefan J., Schustak, Joshua, Sharp, Phillip A., Hynes, Richard O.
We report a system for Cre-regulated expression of RNA interference in vivo. Expression cassettes comprise selectable and FACS-sortable markers in tandem with additional marker genes and shRNAs in...