Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells (2005)
Lina E. Tze, Brian R. Schram, Kong-Peng Lam, Kristin A. Hogquist, Keli L. Hippen, Jiabin Liu, ...
Gene rearrangement is a hallmark of B cell maturation. By interrupting basal cell signaling through the rearranged IgM receptor, immature B cells "back-differentiate" to an earlier stage in their...
Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells (2005)
Lina E. Tze, Brian R. Schram, Kong-Peng Lam, Kristin A. Hogquist, Keli L. Hippen, Jiabin Liu, ...
In developing B lymphocytes, a successful V(D)J heavy chain (HC) immunoglobulin (Ig) rearrangement establishes HC allelic exclusion and signals pro-B cells to advance in development to the pre-B...
Shinjo, Fukiko, Hardy, Richard R., Jongstra, Jan
mAbs specific for mouse λ5 protein were prepared by fusion of spleen cells from a hamster Immunized with recomblnant λ5 protein synthesized in bacteria and the mouse myeloma cell line...
Reichman-Fried, Michal, Bosma, Melvin J., Hardy, Richard R., Phillips, R A.
The severe combined immunodeficiency (scid) mouse mutation impairs the recombination of Ig and TCR genes. Mice homozygous for this mutation (scid mice) lack pre-B, B, and T lymphocytes. Earlier we...
The 3G11+ antigen, a marker for murine CD4+ TH1 lymphocytes, is a ganglioside (1993)
Greer, James M., Koerner, Theodore A.W., Hayakawa, Kyoko, Hardy, Richard R., Kemp, John D.
Two monoclonal antibodies (mAbs), SM3G11 and SM6C10, can be used to discriminate between functionally distinct murine CD4+ T cell subsets. In this study, we use high-performance thin-layer...
HD mice: A novel mouse mutant with a specific defect in the generation of CD4+ T cells
Dave, Vibhuti P., Allman, David, Keefe, Robert, Hardy, Richard R., Kappes, Dietmar J.
We have identified a spontaneous mutation in mice, which we term HD for “helper T cell deficient.” This mouse is distinguished by the virtual absence of peripheral T cells of the CD4+8− major...
Human chronic lymphocytic leukemia modeled in mouse by targeted TCL1 expression
Bichi, Roberta, Shinton, Susan A., Martin, Eric S., Koval, Anatoliy, Calin, George A., Cesari, Rossano, ...
The TCL1 gene at 14q32.1 is involved in chromosomal translocations and inversions in mature T cell leukemias. These leukemias are classified either as T prolymphocytic leukemias, which occur very...
Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells
Tze, Lina E, Schram, Brian R, Lam, Kong-Peng, Hogquist, Kristin A, Hippen, Keli L, Liu, Jiabin, ...
In developing B lymphocytes, a successful V(D)J heavy chain (HC) immunoglobulin (Ig) rearrangement establishes HC allelic exclusion and signals pro-B cells to advance in development to the pre-B...
HD mice: A novel mouse mutant with a specific defect in the generation of CD4+ T cells
Dave, Vibhuti P., Allman, David, Keefe, Robert, Hardy, Richard R., Kappes, Dietmar J.
We have identified a spontaneous mutation in mice, which we term HD for “helper T cell deficient.” This mouse is distinguished by the virtual absence of peripheral T cells of the CD4+8− major...
Human chronic lymphocytic leukemia modeled in mouse by targeted TCL1 expression
Bichi, Roberta, Shinton, Susan A., Martin, Eric S., Koval, Anatoliy, Calin, George A., Cesari, Rossano, ...
The TCL1 gene at 14q32.1 is involved in chromosomal translocations and inversions in mature T cell leukemias. These leukemias are classified either as T prolymphocytic leukemias, which occur very...
Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells
Tze, Lina E, Schram, Brian R, Lam, Kong-Peng, Hogquist, Kristin A, Hippen, Keli L, Liu, Jiabin, ...
In developing B lymphocytes, a successful V(D)J heavy chain (HC) immunoglobulin (Ig) rearrangement establishes HC allelic exclusion and signals pro-B cells to advance in development to the pre-B...
Lineage specification and plasticity in CD19− early B cell precursors
Rumfelt, Lynn L., Zhou, Yan, Rowley, Benjamin M., Shinton, Susan A., Hardy, Richard R.
We describe here three CD19− B cell precursor populations in mouse bone marrow identified using 12-color flow cytometry. Cell transfer experiments indicate lineage potentials consistent with...
Hayakawa, Kyoko, Asano, Masanao, Shinton, Susan A., Gui, Ming, Wen, Li-Jun, Dashoff, Joni, ...
A natural serum autoantibody specific for the Thy-1 glycoprotein (anti–Thy-1 autoantibody [ATA]) is produced by B-1 cells that are positively selected by self-antigen. Here, using ATAμκ...
Commitment to the B Lymphoid Lineage Occurs before DH-JH Recombination
Allman, David, Li, Jin, Hardy, Richard R.
Lineage commitment in B lymphopoiesis remains poorly understood due to the inability to clearly define newly committed B lineage progenitors and their multipotential descendants. We examined the...
Developmental Switches in Chemokine Response Profiles during B Cell Differentiation and Maturation
Bowman, Edward P., Campbell, James J., Soler, Dulce, Dong, Zengjun, Manlongat, Natasha, Picarella, Dominic, ...
Developing B cells undergo dramatic changes in their responses to chemoattractant cytokines (chemokines) and in expression of chemokine receptors. Bone marrow pre–pro-B cells (AA4.1+/natural killer...
Regulation of Anti-DNA B Cells in Recombination-activating Gene–deficient Mice
Xu, Hui, Li, Hui, Suri-Payer, Elisabeth, Hardy, Richard R., Weigert, Martin
Anti-DNA antibodies are regulated in normal individuals but are found in high concentration in the serum of systemic lupus erythematosus (SLE) patients and the MRL lpr/lpr mouse model of SLE. We...
Giambra, Vincenzo, Volpi, Sabrina, Emelyanov, Alexander V., Pflugh, David, Bothwell, Alfred L. M., Norio, Paolo, ...
The 3′ regulatory region (3′ RR) of the murine immunoglobulin heavy chain (IgH) locus contains multiple DNase I-hypersensitive (hs) sites. Proximal sites hs3A, hs1.2, and hs3B are located in an...
Amin, Rupesh H., Cado, Dragana, Nolla, Hector, Huang, Dan, Shinton, Susan A., Zhou, Yan, ...
Allelic exclusion of Ig gene expression is necessary to limit the number of functional receptors to one per B cell. The mechanism underlying allelic exclusion is unknown. Because germline...