Robert A. LaRossa

Publication List Details

Period

2000 - 2000

Number

25

Co-Authors

In Vivo Titration of Mitomycin C Action by Four Escherichia coli Genomic Regions on (2000)

Multicopy Plasmids, Yan Wei, Amy C. Vollmer, Robert A. Larossa

Mitomycin C (MMC), a DNA-damaging agent, is a potent inducer of the bacterial SOS response; surprisingly, it has not been used to select resistant mutants from wild-type Escherichia coli. MMC...

A genomic approach to gene fusion technology

Van Dyk, Tina K., Wei, Yan, Hanafey, Michael K., Dolan, Maureen, Reeve, Mary Jane G., Rafalski, J. Antoni, ...

Gene expression profiling provides powerful analyses of transcriptional responses to cellular perturbation. In contrast to DNA array-based methods, reporter gene technology has been underused for...

High-Density Microarray-Mediated Gene Expression Profiling of Escherichia coli

Wei, Yan, Lee, Jian-Ming, Richmond, Craig, Blattner, Frederick R., Rafalski, J. Antoni, LaRossa, Robert A.

A nearly complete collection of 4,290 Escherichia coli open reading frames was amplified and arrayed in high density on glass slides. To exploit this reagent, conditions for RNA isolation from E....

In Vivo Titration of Mitomycin C Action by Four Escherichia coli Genomic Regions on Multicopy Plasmids

Wei, Yan, Vollmer, Amy C., LaRossa, Robert A.

Mitomycin C (MMC), a DNA-damaging agent, is a potent inducer of the bacterial SOS response; surprisingly, it has not been used to select resistant mutants from wild-type Escherichia coli. MMC...

Global Impact of sdiA Amplification Revealed by Comprehensive Gene Expression Profiling of Escherichia coli

Wei, Yan, Lee, Jian-Ming, Smulski, Dana R., LaRossa, Robert A.

In Escherichia coli the amplification of sdiA, a positive activator of ftsQAZ, genes that are essential for septation, results in mitomycin C resistance. To help us understand this resistance...

DNA Microarray-Mediated Transcriptional Profiling of the Escherichia coli Response to Hydrogen Peroxide

Zheng, Ming, Wang, Xunde, Templeton, Lori J., Smulski, Dana R., LaRossa, Robert A., Storz, Gisela

The genome-wide transcription profile of Escherichia coli cells treated with hydrogen peroxide was examined with a DNA microarray composed of 4,169 E. coli open reading frames. By measuring gene...

Combined, Functional Genomic-Biochemical Approach to Intermediary Metabolism: Interaction of Acivicin, a Glutamine Amidotransferase Inhibitor, with Escherichia coli K-12

Smulski, Dana R., Huang, Lixuan L., McCluskey, Michael P., Reeve, Mary Jane Gladnick, Vollmer, Amy C., Van Dyk, Tina K., ...

Acivicin, a modified amino acid natural product, is a glutamine analog. Thus, it might interfere with metabolism by hindering glutamine transport, formation, or usage in processes such as...

Constricted Flux through the Branched-Chain Amino Acid Biosynthetic Enzyme Acetolactate Synthase Triggers Elevated Expression of Genes Regulated by rpoS and Internal Acidification

Van Dyk, Tina K., Ayers, Brenda L., Morgan, Robin W., Larossa, Robert A.

The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the...

Branched-Chain Amino Acid Biosynthesis in Salmonella typhimurium: a Quantitative Analysis

Epelbaum, Sabine, LaRossa, Robert A., VanDyk, Tina K., Elkayam, T., Chipman, David M., Barak, Ze’ev

We report here the first quantitative study of the branched-chain amino acid biosynthetic pathway in Salmonella typhimurium LT2. The intracellular levels of the enzymes of the pathway and of the...

Global Gene Expression Profiles of the Cyanobacterium Synechocystis sp. Strain PCC 6803 in Response to Irradiation with UV-B and White Light

Huang, Lixuan, McCluskey, Michael P., Ni, Hao, LaRossa, Robert A.

We developed a transcript profiling methodology to elucidate expression patterns of the cyanobacterium Synechocystis sp. strain PCC 6803 and used the technology to investigate changes in gene...

Interfering with Different Steps of Protein Synthesis Explored by Transcriptional Profiling of Escherichia coli K-12†

Sabina, Jeffrey, Dover, Nir, Templeton, Lori J., Smulski, Dana R., Söll, Dieter, LaRossa, Robert A.

Escherichia coli responses to four inhibitors that interfere with translation were monitored at the transcriptional level. A DNA microarray method provided a comprehensive view of changes in mRNA...

Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species

Mukhopadhyay, Partha, Zheng, Ming, Bedzyk, Laura A., LaRossa, Robert A., Storz, Gisela

We examined the genomewide transcriptional responses of Escherichia coli treated with nitrosylated glutathione or the nitric oxide (NO)-generator acidified sodium nitrite (NaNO2) during aerobic...

A genomic approach to gene fusion technology

Van Dyk, Tina K., Wei, Yan, Hanafey, Michael K., Dolan, Maureen, Reeve, Mary Jane G., Rafalski, J. Antoni, ...

Gene expression profiling provides powerful analyses of transcriptional responses to cellular perturbation. In contrast to DNA array-based methods, reporter gene technology has been underused for...

High-Density Microarray-Mediated Gene Expression Profiling of Escherichia coli

Wei, Yan, Lee, Jian-Ming, Richmond, Craig, Blattner, Frederick R., Rafalski, J. Antoni, LaRossa, Robert A.

A nearly complete collection of 4,290 Escherichia coli open reading frames was amplified and arrayed in high density on glass slides. To exploit this reagent, conditions for RNA isolation from E....

In Vivo Titration of Mitomycin C Action by Four Escherichia coli Genomic Regions on Multicopy Plasmids

Wei, Yan, Vollmer, Amy C., LaRossa, Robert A.

Mitomycin C (MMC), a DNA-damaging agent, is a potent inducer of the bacterial SOS response; surprisingly, it has not been used to select resistant mutants from wild-type Escherichia coli. MMC...

Global Impact of sdiA Amplification Revealed by Comprehensive Gene Expression Profiling of Escherichia coli

Wei, Yan, Lee, Jian-Ming, Smulski, Dana R., LaRossa, Robert A.

In Escherichia coli the amplification of sdiA, a positive activator of ftsQAZ, genes that are essential for septation, results in mitomycin C resistance. To help us understand this resistance...

DNA Microarray-Mediated Transcriptional Profiling of the Escherichia coli Response to Hydrogen Peroxide

Zheng, Ming, Wang, Xunde, Templeton, Lori J., Smulski, Dana R., LaRossa, Robert A., Storz, Gisela

The genome-wide transcription profile of Escherichia coli cells treated with hydrogen peroxide was examined with a DNA microarray composed of 4,169 E. coli open reading frames. By measuring gene...

Combined, Functional Genomic-Biochemical Approach to Intermediary Metabolism: Interaction of Acivicin, a Glutamine Amidotransferase Inhibitor, with Escherichia coli K-12

Smulski, Dana R., Huang, Lixuan L., McCluskey, Michael P., Reeve, Mary Jane Gladnick, Vollmer, Amy C., Van Dyk, Tina K., ...

Acivicin, a modified amino acid natural product, is a glutamine analog. Thus, it might interfere with metabolism by hindering glutamine transport, formation, or usage in processes such as...

Constricted Flux through the Branched-Chain Amino Acid Biosynthetic Enzyme Acetolactate Synthase Triggers Elevated Expression of Genes Regulated by rpoS and Internal Acidification

Van Dyk, Tina K., Ayers, Brenda L., Morgan, Robin W., Larossa, Robert A.

The first common enzyme of isoleucine and valine biosynthesis, acetolactate synthase (ALS), is specifically inhibited by the herbicide sulfometuron methyl (SM). To further understand the...

Branched-Chain Amino Acid Biosynthesis in Salmonella typhimurium: a Quantitative Analysis

Epelbaum, Sabine, LaRossa, Robert A., VanDyk, Tina K., Elkayam, T., Chipman, David M., Barak, Ze’ev

We report here the first quantitative study of the branched-chain amino acid biosynthetic pathway in Salmonella typhimurium LT2. The intracellular levels of the enzymes of the pathway and of the...

Global Gene Expression Profiles of the Cyanobacterium Synechocystis sp. Strain PCC 6803 in Response to Irradiation with UV-B and White Light

Huang, Lixuan, McCluskey, Michael P., Ni, Hao, LaRossa, Robert A.

We developed a transcript profiling methodology to elucidate expression patterns of the cyanobacterium Synechocystis sp. strain PCC 6803 and used the technology to investigate changes in gene...

Interfering with Different Steps of Protein Synthesis Explored by Transcriptional Profiling of Escherichia coli K-12†

Sabina, Jeffrey, Dover, Nir, Templeton, Lori J., Smulski, Dana R., Söll, Dieter, LaRossa, Robert A.

Escherichia coli responses to four inhibitors that interfere with translation were monitored at the transcriptional level. A DNA microarray method provided a comprehensive view of changes in mRNA...

Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species

Mukhopadhyay, Partha, Zheng, Ming, Bedzyk, Laura A., LaRossa, Robert A., Storz, Gisela

We examined the genomewide transcriptional responses of Escherichia coli treated with nitrosylated glutathione or the nitric oxide (NO)-generator acidified sodium nitrite (NaNO2) during aerobic...

Quality control despite mistranslation caused by an ambiguous genetic code

Ruan, Benfang, Palioura, Sotiria, Sabina, Jeffrey, Marvin-Guy, Laure, Kochhar, Sunil, LaRossa, Robert A., ...

A high level of accuracy during protein synthesis is considered essential for life. Aminoacyl-tRNA synthetases (aaRSs) translate the genetic code by ensuring the correct pairing of amino acids with...