Comparative analysis of H2A.Z nucleosome organization in the human and yeast genomes (2009)
Tolstorukov, Michael Y., Kharchenko, Peter V., Goldman, Joseph A., Kingston, Robert E., Park, Peter J.
Eukaryotic DNA is wrapped around a histone protein core to constitute the fundamental repeating units of chromatin, the nucleosomes. The affinity of the histone core for DNA depends on the nucleotide...
Bouazoune, Karim, Miranda, Tina B., Jones, Peter A., Kingston, Robert E.
Chromatin remodeling enzymes use the energy of ATP hydrolysis to alter histone–DNA contacts and regulate DNA-based processes in eukaryotes. Whether different subfamilies of remodeling complexes...
Structural basis of histone H4 recognition by p55 (2008)
Song, Ji-Joon, Garlick, Joseph D., Kingston, Robert E.
p55 is a common component of many chromatin-modifying complexes and has been shown to bind to histones. Here, we present a crystal structure of Drosophila p55 bound to a histone H4 peptide. p55, a...
Nucleosome positioning in human HOX gene clusters (2008)
Kharchenko, Peter V., Woo, Caroline J., Tolstorukov, Michael Y., Kingston, Robert E., Park, Peter J.
The distribution of nucleosomes along the genome is a significant aspect of chromatin structure and is thought to influence gene regulation through modulation of DNA accessibility. However,...
Dennis, Jonathan H., Fan, Hua-Ying, Reynolds, Sheila M., Yuan, Guocheng, Meldrim, James C., Richter, Daniel J., ...
The fundamental building block of chromatin, the nucleosome, occupies 150 bp of DNA in a spaced arrangement that is a primary determinant in regulation of the genome. The nucleosomal organization of...
Corey, Laura L., Weirich, Christine S., Benjamin, Ivor J., Kingston, Robert E.
To understand the role of chromatin-remodeling activities in transcription, it is necessary to understand how they interact with transcriptional activators in vivo to regulate the different steps of...
Mulholland, Niveen M., King, Ian F.G., Kingston, Robert E.
Repression and activation of the expression of homeotic genes are maintained by proteins encoded by the Polycomb group (PcG) and trithorax group (trxG) genes. Complexes formed by these proteins are...
Schnitzler, Gavin R., Cheung, Chin Li, Hafner, Jason H., Saurin, Andrew J., Kingston, Robert E., Lieber, Charles M.
Chromatin-remodeling complexes alter chromatin structure to facilitate, or in some cases repress, gene expression. Recent studies have suggested two potential pathways by which such regulation might...
Mammalian SWI-SNF complexes contribute to activation of the hsp70 gene (2000)
De La Serna, Ivana L., Carlson, Kerri A., Hill, David A., Guidi, Cynthia J., Stephenson, Ryan O., Sif, Said, ...
ATP-dependent chromatin-remodeling complexes are conserved among all eukaryotes and function by altering nucleosome structure to allow cellular regulatory factors access to the DNA. Mammalian SWI-SNF...
Functional selectivity of recombinant mammalian SWI/SNF subunits (2000)
Kadam, Shilpa, McAlpine, Glenn S., Phelan, Michael L., Kingston, Robert E., Jones, Katherine A., Emerson, Beverly M.
Mitotic inactivation of a human SWI/SNF chromatin remodeling complex (1998)
Sif, Saïd, Stukenberg, P. Todd, Kirschner, Marc W., Kingston, Robert E.
Bunker, Christopher A., Kingston, Robert E.
We describe a system for screening cDNA expression libraries in Escherichia coli based on protein-protein interactions. The system utilizes fusion proteins containing the DNA binding domain of the...
Mammalian promoter element function in the fission yeast Schizosaccharomyces pombe (1992)
Prentice, Holly L., Kingston, Robert E.
We have analyzed the function of several mammalian promoter elements in the fission yeast, Schizosaccharomyces pombe. Mutants of the human HSP70 promoter were introduced into S. pombe as single copy...
Stable Remodeling of Tailless Nucleosomes by the Human SWI-SNF Complex†
Guyon, Jeffrey R., Narlikar, Geeta J., Sif, Saïd, Kingston, Robert E.
The histone N-terminal tails have been shown previously to be important for chromatin assembly, remodeling, and stability. We have tested the ability of human SWI-SNF (hSWI-SNF) to remodel...
Mammalian SWI-SNF Complexes Contribute to Activation of the hsp70 Gene
De La Serna, Ivana L., Carlson, Kerri A., Hill, David A., Guidi, Cynthia J., Stephenson, Ryan O., Sif, Saïd, ...
ATP-dependent chromatin-remodeling complexes are conserved among all eukaryotes and function by altering nucleosome structure to allow cellular regulatory factors access to the DNA. Mammalian SWI-SNF...
Phelan, Michael L., Schnitzler, Gavin R., Kingston, Robert E.
Chromatin remodeling complexes help regulate the structure of chromatin to facilitate transcription. The multisubunit human (h) SWI-SNF complex has been shown to remodel mono- and polynucleosome...
Citterio, Elisabetta, Van Den Boom, Vincent, Schnitzler, Gavin, Kanaar, Roland, Bonte, Edgar, Kingston, Robert E., ...
The Cockayne syndrome B protein (CSB) is required for coupling DNA excision repair to transcription in a process known as transcription-coupled repair (TCR). Cockayne syndrome patients show UV...
Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF
Sullivan, E. Kelly, Weirich, Christine S., Guyon, Jeffrey R., Sif, Saïd, Kingston, Robert E.
Chromatin remodeling complexes such as SWI/SNF use the energy of ATP hydrolysis to remodel nucleosomal DNA and increase transcription of nucleosomal templates. Human heat shock factor one (hHSF1) is...
Stability of a Human SWI-SNF Remodeled Nucleosomal Array
Guyon, Jeffrey R., Narlikar, Geeta J., Sullivan, E. Kelly, Kingston, Robert E.
SWI-SNF alters DNA-histone interactions within a nucleosome in an ATP-dependent manner. These alterations cause changes in the topology of a closed circular nucleosomal array that persist after...
Schnitzler, Gavin R., Cheung, Chin Li, Hafner, Jason H., Saurin, Andrew J., Kingston, Robert E., Lieber, Charles M.
Chromatin-remodeling complexes alter chromatin structure to facilitate, or in some cases repress, gene expression. Recent studies have suggested two potential pathways by which such regulation might...
Aoyagi, Sayura, Narlikar, Geeta, Zheng, Chunyang, Sif, Saïd, Kingston, Robert E., Hayes, Jeffrey J.
We utilized a site-specific cross-linking technique to investigate the mechanism of nucleosome remodeling by hSWI/SNF. We found that a single cross-link between H2B and DNA virtually eliminates the...
Levine, Stuart S., Weiss, Alona, Erdjument-Bromage, Hediye, Shao, Zhaohui, Tempst, Paul, Kingston, Robert E.
The Polycomb group (PcG) genes are required to maintain homeotic genes in a silenced state during development in drosophila and mammals and are thought to form several distinct silencing complexes...
King, Ian F. G., Francis, Nicole J., Kingston, Robert E.
Polycomb group (PcG) proteins are responsible for stable repression of homeotic gene expression during Drosophila melanogaster development. They are thought to stabilize chromatin structure to...
Corey, Laura L., Weirich, Christine S., Benjamin, Ivor J., Kingston, Robert E.
To understand the role of chromatin-remodeling activities in transcription, it is necessary to understand how they interact with transcriptional activators in vivo to regulate the different steps of...
Regulation of Polycomb group complexes by the sequence-specific DNA binding proteins Zeste and GAGA
Mulholland, Niveen M., King, Ian F.G., Kingston, Robert E.
Repression and activation of the expression of homeotic genes are maintained by proteins encoded by the Polycomb group (PcG) and trithorax group (trxG) genes. Complexes formed by these proteins are...
Disruption of downstream chromatin directed by a transcriptional activator
Brown, Steven A., Kingston, Robert E.
Promoter–proximal pausing during transcriptional elongation is an important way of regulating many diverse loci, including the human hsp70 gene. Pausing of RNA polymerase can be enhanced by...
Functional selectivity of recombinant mammalian SWI/SNF subunits
Kadam, Shilpa, McAlpine, Glenn S., Phelan, Michael L., Kingston, Robert E., Jones, Katherine A., Emerson, Beverly M.
The SWI/SNF family of chromatin-remodeling complexes plays a key role in facilitating the binding of specific transcription factors to nucleosomal DNA in diverse organisms from yeast to man. Yet the...
Mitotic inactivation of a human SWI/SNF chromatin remodeling complex
Sif, Saïd, Stukenberg, P. Todd, Kirschner, Marc W., Kingston, Robert E.
During mitosis, chromatin is condensed into mitotic chromosomes and transcription is inhibited, processes that might be opposed by the chromatin remodeling activity of the SWI/SNF complexes. Brg1 and...
Trinklein, Nathan D., Chen, Will C., Kingston, Robert E., Myers, Richard M.
Transcription of mammalian heat shock genes can be regulated by heat shock factors (HSF) 1 and 2. Although it has been shown previously that these factors respond to distinct stimuli, a broad...
King, Ian F. G., Emmons, Richard B., Francis, Nicole J., Wild, Brigitte, Müller, Jürg, Kingston, Robert E., ...
Polycomb group (PcG) genes propagate patterns of transcriptional repression throughout development. The products of several such genes are part of Polycomb repressive complex 1 (PRC1), which inhibits...
Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes
Sif, Saïd, Saurin, Andrew J., Imbalzano, Anthony N., Kingston, Robert E.
Alteration of nucleosomes by ATP-dependent remodeling complexes represents a critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that...
Mahajan, Milind C., Narlikar, Geeta J., Boyapaty, Gokul, Kingston, Robert E., Weissman, Sherman M.
Locus control regions (LCRs) are regulatory DNA sequences that are situated many kilobases away from their cognate promoters. LCRs protect transgenes from position effect variegation and...
Requirement for sex comb on midleg protein interactions in Drosophila polycomb group repression.
Peterson, Aidan J, Mallin, Daniel R, Francis, Nicole J, Ketel, Carrie S, Stamm, Joyce, Voeller, Rochus K, ...
The Drosophila Sex Comb on Midleg (SCM) protein is a transcriptional repressor of the Polycomb group (PcG). Although genetic studies establish SCM as a crucial PcG member, its molecular role is not...
Stable Remodeling of Tailless Nucleosomes by the Human SWI-SNF Complex†
Guyon, Jeffrey R., Narlikar, Geeta J., Sif, Saïd, Kingston, Robert E.
The histone N-terminal tails have been shown previously to be important for chromatin assembly, remodeling, and stability. We have tested the ability of human SWI-SNF (hSWI-SNF) to remodel...
Mammalian SWI-SNF Complexes Contribute to Activation of the hsp70 Gene
De La Serna, Ivana L., Carlson, Kerri A., Hill, David A., Guidi, Cynthia J., Stephenson, Ryan O., Sif, Saïd, ...
ATP-dependent chromatin-remodeling complexes are conserved among all eukaryotes and function by altering nucleosome structure to allow cellular regulatory factors access to the DNA. Mammalian SWI-SNF...
Phelan, Michael L., Schnitzler, Gavin R., Kingston, Robert E.
Chromatin remodeling complexes help regulate the structure of chromatin to facilitate transcription. The multisubunit human (h) SWI-SNF complex has been shown to remodel mono- and polynucleosome...
Citterio, Elisabetta, Van Den Boom, Vincent, Schnitzler, Gavin, Kanaar, Roland, Bonte, Edgar, Kingston, Robert E., ...
The Cockayne syndrome B protein (CSB) is required for coupling DNA excision repair to transcription in a process known as transcription-coupled repair (TCR). Cockayne syndrome patients show UV...
Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF
Sullivan, E. Kelly, Weirich, Christine S., Guyon, Jeffrey R., Sif, Saïd, Kingston, Robert E.
Chromatin remodeling complexes such as SWI/SNF use the energy of ATP hydrolysis to remodel nucleosomal DNA and increase transcription of nucleosomal templates. Human heat shock factor one (hHSF1) is...
Stability of a Human SWI-SNF Remodeled Nucleosomal Array
Guyon, Jeffrey R., Narlikar, Geeta J., Sullivan, E. Kelly, Kingston, Robert E.
SWI-SNF alters DNA-histone interactions within a nucleosome in an ATP-dependent manner. These alterations cause changes in the topology of a closed circular nucleosomal array that persist after...
Schnitzler, Gavin R., Cheung, Chin Li, Hafner, Jason H., Saurin, Andrew J., Kingston, Robert E., Lieber, Charles M.
Chromatin-remodeling complexes alter chromatin structure to facilitate, or in some cases repress, gene expression. Recent studies have suggested two potential pathways by which such regulation might...
Aoyagi, Sayura, Narlikar, Geeta, Zheng, Chunyang, Sif, Saïd, Kingston, Robert E., Hayes, Jeffrey J.
We utilized a site-specific cross-linking technique to investigate the mechanism of nucleosome remodeling by hSWI/SNF. We found that a single cross-link between H2B and DNA virtually eliminates the...
Levine, Stuart S., Weiss, Alona, Erdjument-Bromage, Hediye, Shao, Zhaohui, Tempst, Paul, Kingston, Robert E.
The Polycomb group (PcG) genes are required to maintain homeotic genes in a silenced state during development in drosophila and mammals and are thought to form several distinct silencing complexes...
King, Ian F. G., Francis, Nicole J., Kingston, Robert E.
Polycomb group (PcG) proteins are responsible for stable repression of homeotic gene expression during Drosophila melanogaster development. They are thought to stabilize chromatin structure to...
Corey, Laura L., Weirich, Christine S., Benjamin, Ivor J., Kingston, Robert E.
To understand the role of chromatin-remodeling activities in transcription, it is necessary to understand how they interact with transcriptional activators in vivo to regulate the different steps of...
Regulation of Polycomb group complexes by the sequence-specific DNA binding proteins Zeste and GAGA
Mulholland, Niveen M., King, Ian F.G., Kingston, Robert E.
Repression and activation of the expression of homeotic genes are maintained by proteins encoded by the Polycomb group (PcG) and trithorax group (trxG) genes. Complexes formed by these proteins are...
Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes
Sif, Saïd, Saurin, Andrew J., Imbalzano, Anthony N., Kingston, Robert E.
Alteration of nucleosomes by ATP-dependent remodeling complexes represents a critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that...
Disruption of downstream chromatin directed by a transcriptional activator
Brown, Steven A., Kingston, Robert E.
Promoter–proximal pausing during transcriptional elongation is an important way of regulating many diverse loci, including the human hsp70 gene. Pausing of RNA polymerase can be enhanced by...
Functional selectivity of recombinant mammalian SWI/SNF subunits
Kadam, Shilpa, McAlpine, Glenn S., Phelan, Michael L., Kingston, Robert E., Jones, Katherine A., Emerson, Beverly M.
The SWI/SNF family of chromatin-remodeling complexes plays a key role in facilitating the binding of specific transcription factors to nucleosomal DNA in diverse organisms from yeast to man. Yet the...
Mitotic inactivation of a human SWI/SNF chromatin remodeling complex
Sif, Saïd, Stukenberg, P. Todd, Kirschner, Marc W., Kingston, Robert E.
During mitosis, chromatin is condensed into mitotic chromosomes and transcription is inhibited, processes that might be opposed by the chromatin remodeling activity of the SWI/SNF complexes. Brg1 and...
Trinklein, Nathan D., Chen, Will C., Kingston, Robert E., Myers, Richard M.
Transcription of mammalian heat shock genes can be regulated by heat shock factors (HSF) 1 and 2. Although it has been shown previously that these factors respond to distinct stimuli, a broad...
King, Ian F. G., Emmons, Richard B., Francis, Nicole J., Wild, Brigitte, Müller, Jürg, Kingston, Robert E., ...
Polycomb group (PcG) genes propagate patterns of transcriptional repression throughout development. The products of several such genes are part of Polycomb repressive complex 1 (PRC1), which inhibits...
Mahajan, Milind C., Narlikar, Geeta J., Boyapaty, Gokul, Kingston, Robert E., Weissman, Sherman M.
Locus control regions (LCRs) are regulatory DNA sequences that are situated many kilobases away from their cognate promoters. LCRs protect transgenes from position effect variegation and...
Requirement for sex comb on midleg protein interactions in Drosophila polycomb group repression.
Peterson, Aidan J, Mallin, Daniel R, Francis, Nicole J, Ketel, Carrie S, Stamm, Joyce, Voeller, Rochus K, ...
The Drosophila Sex Comb on Midleg (SCM) protein is a transcriptional repressor of the Polycomb group (PcG). Although genetic studies establish SCM as a crucial PcG member, its molecular role is not...
Independent and complementary methods for large-scale structural analysis of mammalian chromatin
Dennis, Jonathan H., Fan, Hua-Ying, Reynolds, Sheila M., Yuan, Guocheng, Meldrim, James C., Richter, Daniel J., ...
The fundamental building block of chromatin, the nucleosome, occupies 150 bp of DNA in a spaced arrangement that is a primary determinant in regulation of the genome. The nucleosomal organization of...
Trotter, Kevin W., Fan, Hua-Ying, Ivey, Melissa L., Kingston, Robert E., Archer, Trevor K.
The packaging of eukaryotic DNA into chromatin can create an impediment to transcription by hindering binding of essential factors required for transcription. The mammalian SWI/SNF remodeling complex...
Structural basis of histone H4 recognition by p55
Song, Ji-Joon, Garlick, Joseph D., Kingston, Robert E.
p55 is a common component of many chromatin-modifying complexes and has been shown to bind to histones. Here, we present a crystal structure of Drosophila p55 bound to a histone H4 peptide. p55, a...
Nucleosome positioning in human HOX gene clusters
Kharchenko, Peter V., Woo, Caroline J., Tolstorukov, Michael Y., Kingston, Robert E., Park, Peter J.
The distribution of nucleosomes along the genome is a significant aspect of chromatin structure and is thought to influence gene regulation through modulation of DNA accessibility. However,...
Koh, Andrew S., Kuo, Alex J., Park, Sang Youn, Cheung, Peggie, Abramson, Jakub, Bua, Dennis, ...
Aire induces ectopic expression of peripheral tissue antigens (PTAs) in thymic medullary epithelial cells, which promotes immunological tolerance. Beginning with a broad screen of histone peptides,...
Bouazoune, Karim, Miranda, Tina B., Jones, Peter A., Kingston, Robert E.
Chromatin remodeling enzymes use the energy of ATP hydrolysis to alter histone–DNA contacts and regulate DNA-based processes in eukaryotes. Whether different subfamilies of remodeling complexes...