Loss of DictyosteliumHSPC300 causes a scar-like phenotype and loss of SCAR protein (2009)
Pollitt, Alice Y, Insall, Robert H
Abstract Background SCAR/WAVE proteins couple signalling to actin polymerization, and are thus fundamental to the formation of pseudopods and lamellipods. They are controlled as part of a...
Zaki, Mehreen, King, Jason, Fütterer, Klaus, Insall, Robert H
Abstract Background Cell motility is an essential feature of the pathogenesis and morbidity of amoebiasis caused by Entamoeba histolytica . As motility depends on cytoskeletal organisation and...
Kae, Helmut, Kortholt, Arjan, Rehmann, Holger, Insall, Robert H., Spiegelman, George B., ...
In general, mammalian Ras guanine nucleotide exchange factors (RasGEFs) show little substrate specificity, although they are often thought to regulate specific pathways. Here, we provide in vitro and...
The Dictyosteliumgenome encodes numerous RasGEFs with multiple biological roles (2005)
Wilkins, Andrew, Szafranski, Karol, Fraser, Derek J, Bakthavatsalam, Deenadayalan, Müller, Rolf, Fisher, Paul R, ...
Abstract Background Dictyostelium discoideum is a eukaryote with a simple lifestyle and a relatively small genome whose sequence has been fully determined. It is widely used for studies on cell...
Gdt2 regulates the transition of Dictyosteliumcells from growth to differentiation (2004)
Chibalina, Margarita V, Anjard, Christophe, Insall, Robert H
Abstract Background Dictyostelium life cycle consists of two distinct phases – growth and development. The control of growth-differentiation transition in Dictyostelium is not completely...
Abstract Background The relative ease of targeted gene disruption in the social amoeba Dictyostelium has stimulated its widespread use as an experimental organism for cell and developmental biology....
Anderson, Stephen I., Behrendt, Barbara, Machesky, Laura M., Insall, Robert H., Nash, Gerard B.
Neutrophils migrate rapidly by co-ordinating regulation of their beta2-integrin adhesion with turnover of filamentous F-actin. The seven-protein Arp2/3 complex regulates actin polymerisation upon...
Dictyostelium RasD is required for normal phototaxis, but not differentiation (2000)
Wilkins, Andrew, Khosla, Meenal, Fraser, Derek J., Spiegelman, George B., Fisher, Paul R., Weeks, Gerald, ...
Ca2+ signalling is not required for chemotaxis in Dictyostelium
Traynor, David, Milne, Jacqueline L.S., Insall, Robert H., Kay, Robert R.
Dictyostelium cells can move rapidly towards a source of cyclic-AMP (cAMP). This chemoattractant is detected by G-protein-linked receptors, which trigger a signalling cascade including a rapid influx...
Dictyostelium RasD is required for normal phototaxis, but not differentiation
Wilkins, Andrew, Khosla, Meenal, Fraser, Derek J., Spiegelman, George B., Fisher, Paul R., Weeks, Gerald, ...
RasD, a Dictyostelium homolog of mammalian Ras, is maximally expressed during the multicellular stage of development. Normal Dictyostelium aggregates are phototactic and thermotactic, moving towards...
Gdt2 regulates the transition of Dictyostelium cells from growth to differentiation
Chibalina, Margarita V, Anjard, Christophe, Insall, Robert H
The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles
Wilkins, Andrew, Szafranski, Karol, Fraser, Derek J, Bakthavatsalam, Deenadayalan, Müller, Rolf, Fisher, Paul R, ...
A survey of the Dictyostelium genome reveals at least 25 RasGEFs, all of which appear to be expressed at some point in development. Disruption of several of these novel RasGEFs reveals that many have...
Ca2+ signalling is not required for chemotaxis in Dictyostelium
Traynor, David, Milne, Jacqueline L.S., Insall, Robert H., Kay, Robert R.
Dictyostelium cells can move rapidly towards a source of cyclic-AMP (cAMP). This chemoattractant is detected by G-protein-linked receptors, which trigger a signalling cascade including a rapid influx...
Dictyostelium RasD is required for normal phototaxis, but not differentiation
Wilkins, Andrew, Khosla, Meenal, Fraser, Derek J., Spiegelman, George B., Fisher, Paul R., Weeks, Gerald, ...
RasD, a Dictyostelium homolog of mammalian Ras, is maximally expressed during the multicellular stage of development. Normal Dictyostelium aggregates are phototactic and thermotactic, moving towards...
Gdt2 regulates the transition of Dictyostelium cells from growth to differentiation
Chibalina, Margarita V, Anjard, Christophe, Insall, Robert H
The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles
Wilkins, Andrew, Szafranski, Karol, Fraser, Derek J, Bakthavatsalam, Deenadayalan, Müller, Rolf, Fisher, Paul R, ...
A survey of the Dictyostelium genome reveals at least 25 RasGEFs, all of which appear to be expressed at some point in development. Disruption of several of these novel RasGEFs reveals that many have...
RacG Regulates Morphology, Phagocytosis, and Chemotaxis▿ †
Somesh, Baggavalli P., Vlahou, Georgia, Iijima, Miho, Insall, Robert H., Devreotes, Peter, Rivero, Francisco
RacG is an unusual member of the complex family of Rho GTPases in Dictyostelium. We have generated a knockout (KO) strain, as well as strains that overexpress wild-type (WT), constitutively active...
Zaki, Mehreen, Andrew, Natalie, Insall, Robert H.
Entamoeba histolytica cells, the cause of amoebic dysentery, are highly motile, and this motility is an essential feature of the pathogenesis and morbidity of amoebiasis. However, the control of E....
Kae, Helmut, Kortholt, Arjan, Rehmann, Holger, Insall, Robert H, Spiegelman, George B, ...
In general, mammalian Ras guanine nucleotide exchange factors (RasGEFs) show little substrate specificity, although they are often thought to regulate specific pathways. Here, we provide in vitro and...
Dictyostelium RasG Is Required for Normal Motility and Cytokinesis, But Not Growth
Tuxworth, Richard I., Cheetham, Janet L., Machesky, Laura M., Spiegelmann, George B., Weeks, Gerald, Insall, Robert H.
RasG is the most abundant Ras protein in growing Dictyostelium cells and the closest relative of mammalian Ras proteins. We have generated null mutants in which expression of RasG is completely...