Robert J. Fisher

Correlation between binding rate constants and individual information of E. coli Fis binding sites (2007)

Shultzaberger, Ryan K., Roberts, Lindsey R., Lyakhov, Ilya G., Sidorov, Igor A., Stephen, Andrew G., Fisher, Robert J., ...

Individual protein binding sites on DNA can be measured in bits of information. This information is related to the free energy of binding by the second law of thermodynamics, but binding kinetics...

Novel high-throughput electrochemiluminescent assay for identification of human tyrosyl-DNA phosphodiesterase (Tdp1) inhibitors and characterization of furamidine (NSC 305831) as an inhibitor of Tdp1 (2007)

Antony, Smitha, Marchand, Christophe, Stephen, Andrew G., Thibaut, Laurent, Agama, Keli K., Fisher, Robert J., ...

By enzymatically hydrolyzing the terminal phosphodiester bond at the 3′-ends of DNA breaks, tyrosyl-DNA phosphodiesterase (Tdp1) repairs topoisomerase-DNA covalent complexes and processes the DNA...

Environmental Technology Verification Coatings and Coating Equipment Program (ETV CCEP). Final Technology Applications Group TAGNITE - Testing and Quality Assurance Plan (T/QAP) (2007)

Fisher, Robert J.

The ETV CCEP verifies the pollution prevention and performance characteristics of coating technologies and makes the results of the testing available to prospective coating technology users. The...

Complex interactions of HIV-1 nucleocapsid protein with oligonucleotides (2006)

Fisher, Robert J., Fivash, Matthew J., Stephen, Andrew G., Hagan, Nathan A., Shenoy, Shilpa R., Medaglia, Maxine V., ...

The HIV-1 nucleocapsid (NC) protein is a small, basic protein containing two retroviral zinc fingers. It is a highly active nucleic acid chaperone; because of this activity, it plays a crucial role...

Nucleic acid binding and chaperone properties of HIV-1 Gag and nucleocapsid proteins (2006)

Cruceanu, Margareta, Urbaneja, Maria A., Hixson, Catherine V., Johnson, Donald G., Datta, Siddhartha A., Fivash, Matthew J., ...

The Gag polyprotein of HIV-1 is essential for retroviral replication and packaging. The nucleocapsid (NC) protein is the primary region for the interaction of Gag with nucleic acids. In this study,...

Method of Firing Ceramics. (1998)

Brown,Arthur D., Fisher,Robert J.

The patent relates to a method of firing ceramic materials in which at least one component is volatile at a temperature below the firing temperature of the ceramic material and particularly, to a...

Neutralizing monoclonal antibodies to hepatocyte growth factor/scatter factor (HGF/SF) display antitumor activity in animal models

Cao, Brian, Su, Yanli, Oskarsson, Marianne, Zhao, Ping, Kort, Eric J., Fisher, Robert J., ...

The hepatocyte growth factor (HGF/SF) receptor, Met, regulates mitogenesis, motility, and morphogenesis in a cell type-dependent fashion. Activation of Met via autocrine, paracrine, or mutational...

Modulation of HIV-like particle assembly in vitro by inositol phosphates

Campbell, Stephen, Fisher, Robert J., Towler, Eric M., Fox, Stephen, Issaq, Haleem J., Wolfe, Tracy, ...

HIV-1 Gag protein assembles into 100- to 120-nm diameter particles in mammalian cells. Recombinant HIV-1 Gag protein assembles in a fully defined system in vitro into particles that are only 25–30...

Trophic Factor Withdrawal: p38 Mitogen-Activated Protein Kinase Activates NHE1, Which Induces Intracellular Alkalinization

Khaled, Annette R., Moor, Andrea N., Li, Aiqun, Kim, Kyungjae, Ferris, Douglas K., Muegge, Kathrin, ...

Trophic factor withdrawal induces cell death by mechanisms that are incompletely understood. Previously we reported that withdrawal of interleukin-7 (IL-7) or IL-3 produced a rapid intracellular...

Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides

Fisher, Robert J., Rein, Alan, Fivash, Matthew, Urbaneja, Maria A., Casas-Finet, José R., Medaglia, Maxine, ...

We have analyzed the binding of recombinant human immunodeficiency virus type 1 nucleocapsid protein (NC) to very short oligonucleotides by using surface plasmon resonance (SPR) technology. Our...

Properties of a Soluble Nitrogenase in Azotobacter

Oppenheim, Joel, Fisher, Robert J., Wilson, P. W., Marcus, Leon

A nitrogenase system that remains in the supernatant fluid after centrifuging for 3 hr at 180,000 × g can be extracted from Azotobacter vinelandii by osmotic lysis of the bacteria. This nitrogenase...

A Soluble Mitochondrial ATP Synthetase Complex Catalyzing ATP-Phosphate and ATP-ADP Exchange

Fisher, Robert J., Chen, Jenn C., Sani, B. P., Kaplay, Suresh S., Sanadi, D. Rao

The highly purified soluble ATP synthetase complex from mitochondria, containing energy-transfer Factor A (the terminal ADP phosphorylation enzyme of oxidative phosphorylation) and Factor D,...

Discovery of Small-Molecule Human Immunodeficiency Virus Type 1 Entry Inhibitors That Target the gp120-Binding Domain of CD4†

Yang, Quan-en, Stephen, Andrew G., Adelsberger, Joseph W., Roberts, Paula E., Zhu, Weimin, Currens, Michael J., ...

The interaction between human immunodeficiency virus type 1 (HIV-1) gp120 and the CD4 receptor is highly specific and involves relatively small contact surfaces on both proteins according to crystal...

Phosphorylation of the protein kinase C-theta activation loop and hydrophobic motif regulates its kinase activity, but only activation loop phosphorylation is critical to in vivo nuclear-factor-kappaB induction.

Liu, Yin, Graham, Caroline, Li, Aiqun, Fisher, Robert J, Shaw, Stephen

Protein kinase C (PKC)-theta, a member of the 'novel' subfamily of PKC isoforms, is of singular importance in transducing signals in T-lymphocytes. Since understanding of regulatory phosphorylation...

Complex interactions of HIV-1 nucleocapsid protein with oligonucleotides

Fisher, Robert J., Fivash, Matthew J., Stephen, Andrew G., Hagan, Nathan A., Shenoy, Shilpa R., Medaglia, Maxine V., ...

The HIV-1 nucleocapsid (NC) protein is a small, basic protein containing two retroviral zinc fingers. It is a highly active nucleic acid chaperone; because of this activity, it plays a crucial role...

Nucleic acid binding and chaperone properties of HIV-1 Gag and nucleocapsid proteins

Cruceanu, Margareta, Urbaneja, Maria A., Hixson, Catherine V., Johnson, Donald G., Datta, Siddhartha A., Fivash, Matthew J., ...

The Gag polyprotein of HIV-1 is essential for retroviral replication and packaging. The nucleocapsid (NC) protein is the primary region for the interaction of Gag with nucleic acids. In this study,...

Neutralizing monoclonal antibodies to hepatocyte growth factor/scatter factor (HGF/SF) display antitumor activity in animal models

Cao, Brian, Su, Yanli, Oskarsson, Marianne, Zhao, Ping, Kort, Eric J., Fisher, Robert J., ...

The hepatocyte growth factor (HGF/SF) receptor, Met, regulates mitogenesis, motility, and morphogenesis in a cell type-dependent fashion. Activation of Met via autocrine, paracrine, or mutational...

Modulation of HIV-like particle assembly in vitro by inositol phosphates

Campbell, Stephen, Fisher, Robert J., Towler, Eric M., Fox, Stephen, Issaq, Haleem J., Wolfe, Tracy, ...

HIV-1 Gag protein assembles into 100- to 120-nm diameter particles in mammalian cells. Recombinant HIV-1 Gag protein assembles in a fully defined system in vitro into particles that are only 25–30...

Trophic Factor Withdrawal: p38 Mitogen-Activated Protein Kinase Activates NHE1, Which Induces Intracellular Alkalinization

Khaled, Annette R., Moor, Andrea N., Li, Aiqun, Kim, Kyungjae, Ferris, Douglas K., Muegge, Kathrin, ...

Trophic factor withdrawal induces cell death by mechanisms that are incompletely understood. Previously we reported that withdrawal of interleukin-7 (IL-7) or IL-3 produced a rapid intracellular...

Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides

Fisher, Robert J., Rein, Alan, Fivash, Matthew, Urbaneja, Maria A., Casas-Finet, José R., Medaglia, Maxine, ...

We have analyzed the binding of recombinant human immunodeficiency virus type 1 nucleocapsid protein (NC) to very short oligonucleotides by using surface plasmon resonance (SPR) technology. Our...

Properties of a Soluble Nitrogenase in Azotobacter

Oppenheim, Joel, Fisher, Robert J., Wilson, P. W., Marcus, Leon

A nitrogenase system that remains in the supernatant fluid after centrifuging for 3 hr at 180,000 × g can be extracted from Azotobacter vinelandii by osmotic lysis of the bacteria. This nitrogenase...

A Soluble Mitochondrial ATP Synthetase Complex Catalyzing ATP-Phosphate and ATP-ADP Exchange

Fisher, Robert J., Chen, Jenn C., Sani, B. P., Kaplay, Suresh S., Sanadi, D. Rao

The highly purified soluble ATP synthetase complex from mitochondria, containing energy-transfer Factor A (the terminal ADP phosphorylation enzyme of oxidative phosphorylation) and Factor D,...

Discovery of Small-Molecule Human Immunodeficiency Virus Type 1 Entry Inhibitors That Target the gp120-Binding Domain of CD4†

Yang, Quan-en, Stephen, Andrew G., Adelsberger, Joseph W., Roberts, Paula E., Zhu, Weimin, Currens, Michael J., ...

The interaction between human immunodeficiency virus type 1 (HIV-1) gp120 and the CD4 receptor is highly specific and involves relatively small contact surfaces on both proteins according to crystal...

Phosphorylation of the protein kinase C-theta activation loop and hydrophobic motif regulates its kinase activity, but only activation loop phosphorylation is critical to in vivo nuclear-factor-kappaB induction.

Liu, Yin, Graham, Caroline, Li, Aiqun, Fisher, Robert J, Shaw, Stephen

Protein kinase C (PKC)-theta, a member of the 'novel' subfamily of PKC isoforms, is of singular importance in transducing signals in T-lymphocytes. Since understanding of regulatory phosphorylation...

Complex interactions of HIV-1 nucleocapsid protein with oligonucleotides

Fisher, Robert J., Fivash, Matthew J., Stephen, Andrew G., Hagan, Nathan A., Shenoy, Shilpa R., Medaglia, Maxine V., ...

The HIV-1 nucleocapsid (NC) protein is a small, basic protein containing two retroviral zinc fingers. It is a highly active nucleic acid chaperone; because of this activity, it plays a crucial role...

Nucleic acid binding and chaperone properties of HIV-1 Gag and nucleocapsid proteins

Cruceanu, Margareta, Urbaneja, Maria A., Hixson, Catherine V., Johnson, Donald G., Datta, Siddhartha A., Fivash, Matthew J., ...

The Gag polyprotein of HIV-1 is essential for retroviral replication and packaging. The nucleocapsid (NC) protein is the primary region for the interaction of Gag with nucleic acids. In this study,...

Proteomic and Biochemical Analysis of Purified Human Immunodeficiency Virus Type 1 Produced from Infected Monocyte-Derived Macrophages

Chertova, Elena, Chertov, Oleg, Coren, Lori V., Roser, James D., Trubey, Charles M., Bess, Julian W., ...

Human immunodeficiency virus type 1 (HIV-1) infects CD4+ T lymphocytes and monocytes/macrophages, incorporating host proteins in the process of assembly and budding. Analysis of the host cell...

Novel high-throughput electrochemiluminescent assay for identification of human tyrosyl-DNA phosphodiesterase (Tdp1) inhibitors and characterization of furamidine (NSC 305831) as an inhibitor of Tdp1

Antony, Smitha, Marchand, Christophe, Stephen, Andrew G., Thibaut, Laurent, Agama, Keli K., Fisher, Robert J., ...

By enzymatically hydrolyzing the terminal phosphodiester bond at the 3′-ends of DNA breaks, tyrosyl-DNA phosphodiesterase (Tdp1) repairs topoisomerase-DNA covalent complexes and processes the DNA...

Correlation between binding rate constants and individual information of E. coli Fis binding sites

Shultzaberger, Ryan K., Roberts, Lindsey R., Lyakhov, Ilya G., Sidorov, Igor A., Stephen, Andrew G., Fisher, Robert J., ...

Individual protein binding sites on DNA can be measured in bits of information. This information is related to the free energy of binding by the second law of thermodynamics, but binding kinetics...

Measuring the Binding Stoichiometry of HIV-1 Gag to Very-Low-Density Oligonucleotide Surfaces Using Surface Plasmon Resonance Spectroscopy

Stephen, Andrew G., Datta, Siddhartha A.K., Worthy, Karen M., Bindu, Lakshman, Fivash, Matthew J., Turner, Kevin B., ...

The interaction of the HIV Gag polyprotein with nucleic acid is a critical step in the assembly of viral particles. The Gag polyprotein is composed of the matrix (MA), capsid (CA), and nucleocapsid...

Quantitative Proteomics Employing Primary Amine Affinity Tags

Hoang, Van M., Conrads, Thomas P., Veenstra, Timothy D., Blonder, Josip, Terunuma, Atsushi, Vogel, Jonathan C., ...

A proteomics-based method using stable isotope labeling to assess the relative abundance of peptides or proteins is described. Bradykinin and carbonic anhydrase were labeled with...

Molecular Characterization of Feline Immunodeficiency Virus Budding▿

Luttge, Benjamin G., Shehu-Xhilaga, Miranda, Demirov, Dimiter G., Adamson, Catherine S., Soheilian, Ferri, Nagashima, Kunio, ...

Infection of domestic cats with feline immunodeficiency virus (FIV) is an important model system for studying human immunodeficiency virus type 1 (HIV-1) infection due to numerous similarities in...

The Effects of Recognition and Group Need on Volunteerism: A Social Norm Perspective.

Fisher, Robert J, Ackerman, David

The significance of volunteering for both individuals and society has lead to numerous studies on this behavior across the social sciences. However, virtually no prior research has evaluated how and...

Normative Influences on Impulsive Buying Behavior.

Rook, Dennis W, Fisher, Robert J

Although consumer researchers have investigated impulse buying for nearly 50 years, almost no research has empirically examined its normative aspects. This article presents conceptual and empirical...

An Investigation into the Social Context of Early Adoption Behavior.

Fisher, Robert J, Price, Linda L

The social context of new product adoption behavior is a key aspect of the diffusion of innovations. Yet little is known about the process by which social contextual factors influence individual...

Gender Differences in Responses to Emotional Advertising: A Social Desirability Perspective

Robert J. Fisher, Laurette Dubé

Two studies examine gender differences in responses to advertising with emotional content that varies on agency-a fundamental component of the male, but not female, stereotype. As hypothesized, males...

An Empathy-Helping Perspective on Consumers' Responses to Fund-Raising Appeals

Robert J. Fisher, Mark Vandenbosch, Kersi D. Antia

The research examines viewers' actual responses to four televised fund-raising drives by a public television station over a 2-year period. The 584 pledge breaks we studied contain 4,868 individual...

Assembly Properties of Human Immunodeficiency Virus Type 1 Gag-Leucine Zipper Chimeras: Implications for Retrovirus Assembly▿

Crist, Rachael M., Datta, Siddhartha A. K., Stephen, Andrew G., Soheilian, Ferri, Mirro, Jane, Fisher, Robert J., ...

Expression of the retroviral Gag protein leads to formation of virus-like particles in mammalian cells. In vitro and in vivo experiments show that nucleic acid is also required for particle assembly....