Bevan, Alison, Brenner, Charles, Fuller, Robert S.
The specificity of the yeast proprotein-processing Kex2 protease was examined in vivo by using a sensitive, quantitative assay. A truncated prepro-α-factor gene encoding an α-factor precursor with...
Optimization of protease-inhibitor interactions by randomizing adventitious contacts
Komiyama, Tomoko, VanderLugt, Bryan, Fugère, Martin, Day, Robert, Kaufman, Randal J., Fuller, Robert S.
Polypeptide protease inhibitors are often found to inhibit targets with which they did not coevolve, as in the case of high-affinity inhibition of bacterial subtilisin by the leech inhibitor eglin c....
Sipos, György, Brickner, Jason H., Brace, E.J., Chen, Linyi, Rambourg, Alain, Kepes, Francois, ...
SOI3 was identified by a mutation, soi3-1, that suppressed a mutant trans-Golgi network (TGN) localization signal in the Kex2p cytosolic tail. SOI3, identical to RAV1, encodes a protein important for...
Komiyama, Tomoko, Swanson, Joel A., Fuller, Robert S.
Cell surface proteolytic processing of anthrax protective antigen by furin or other furin-related proteases is required for its oligomerization, endocytosis, and function as a translocon for anthrax...
Krysan, Damian J., Ting, Elizabeth L., Abeijon, Claudia, Kroos, Lee, Fuller, Robert S.
The yeast cell wall is a crucial extracellular organelle that protects the cell from lysis during environmental stress and morphogenesis. Here, we demonstrate that the yapsin family of five...
Bevan, Alison, Brenner, Charles, Fuller, Robert S.
The specificity of the yeast proprotein-processing Kex2 protease was examined in vivo by using a sensitive, quantitative assay. A truncated prepro-α-factor gene encoding an α-factor precursor with...
Optimization of protease-inhibitor interactions by randomizing adventitious contacts
Komiyama, Tomoko, VanderLugt, Bryan, Fugère, Martin, Day, Robert, Kaufman, Randal J., Fuller, Robert S.
Polypeptide protease inhibitors are often found to inhibit targets with which they did not coevolve, as in the case of high-affinity inhibition of bacterial subtilisin by the leech inhibitor eglin c....
Sipos, György, Brickner, Jason H., Brace, E.J., Chen, Linyi, Rambourg, Alain, Kepes, Francois, ...
SOI3 was identified by a mutation, soi3-1, that suppressed a mutant trans-Golgi network (TGN) localization signal in the Kex2p cytosolic tail. SOI3, identical to RAV1, encodes a protein important for...
Komiyama, Tomoko, Swanson, Joel A., Fuller, Robert S.
Cell surface proteolytic processing of anthrax protective antigen by furin or other furin-related proteases is required for its oligomerization, endocytosis, and function as a translocon for anthrax...
Krysan, Damian J., Ting, Elizabeth L., Abeijon, Claudia, Kroos, Lee, Fuller, Robert S.
The yeast cell wall is a crucial extracellular organelle that protects the cell from lysis during environmental stress and morphogenesis. Here, we demonstrate that the yapsin family of five...
Brace, E. J., Parkinson, Leah P., Fuller, Robert S.
Skp1p is an essential component of SCF-type E3 ubiquitin ligase complexes and associates with these through binding to F-box proteins. Skp1p also binds F-box proteins in a number of non-SCF...
Kean, Leslie S., Grant, Althea M., Angeletti, Cesar, Mahé, Yannick, Kuchler, Karl, Fuller, Robert S., ...
The transcription regulators, PDR1 and PDR3, have been shown to activate the transcription of numerous genes involved in a wide range of functions, including resistance to physical and chemical...
Brickner, Jason H., Fuller, Robert S.
Localization of yeast Kex2 protease to the TGN requires a signal (TLS1) in its cytosolic tail (C-tail). Mutation of TLS1 results in rapid transit of Kex2p to the vacuole. Isolation of suppressors of...
The Tlg SNARE complex is required for TGN homotypic fusion
Brickner, Jason H., Blanchette, Jennifer M., Sipos, György, Fuller, Robert S.
Using a new assay for membrane fusion between late Golgi/endosomal compartments, we have reconstituted a rapid, robust homotypic fusion reaction between membranes containing Kex2p and Ste13p, two...
Abazeed, Mohamed E., Fuller, Robert S.
Golgi-localized, γ-Ear–containing, ADP-ribosylation factor-binding proteins (GGAs) and adaptor protein-1 (AP-1) mediate clathrin-dependent trafficking of transmembrane proteins between the...
Komiyama, Tomoko, Coppola, Julia M., Larsen, Martha J., Van Dort, Marcian E., Ross, Brian D., Day, Robert, ...
Furin is a ubiquitously expressed proprotein convertase (PC) that plays a vital role in numerous disease processes including cancer metastasis, bacterial toxin activation (e.g. anthrax and...