The ClinSeq Project: Piloting large-scale genome sequencing for research in genomic medicine (2009)
Biesecker, Leslie G., Mullikin, James C., Facio, Flavia M., Turner, Clesson, Cherukuri, Praveen F., Blakesley, Robert W., ...
ClinSeq is a pilot project to investigate the use of whole-genome sequencing as a tool for clinical research. By piloting the acquisition of large amounts of DNA sequence data from individual human...
A diversity profile of the human skin microbiota (2008)
Grice, Elizabeth A., Kong, Heidi H., Renaud, Gabriel, Young, Alice C., Bouffard, Gerard G., ...
The many layers and structures of the skin serve as elaborate hosts to microbes, including a diversity of commensal and pathogenic bacteria that contribute to both human health and disease. To...
Margulies, Elliott H., Cooper, Gregory M., Asimenos, George, Thomas, Daryl J., Dewey, Colin N., Siepel, Adam, ...
A key component of the ongoing ENCODE project involves rigorous comparative sequence analyses for the initially targeted 1% of the human genome. Here, we present orthologous sequence generation,...
Morin, Ryan D., Chang, Elbert, Petrescu, Anca, Liao, Nancy, Griffith, Malachi, Kirkpatrick, Robert, ...
Sequencing of full-insert clones from full-length cDNA libraries from both Xenopus laevis and Xenopus tropicalis has been ongoing as part of the Xenopus Gene Collection Initiative. Here we present...
Morin, Ryan D., Chang, Elbert, Petrescu, Anca, Liao, Nancy, Griffith, Malachi, Kirkpatrick, Robert, ...
Sequencing of full-insert clones from full-length cDNA libraries from both Xenopus laevis and Xenopus tropicalis has been ongoing as part of the Xenopus Gene Collection Initiative. Here we present...
An intermediate grade of finished genomic sequence suitable for comparative analyses (2004)
Blakesley, Robert W., Hansen, Nancy F., Mullikin, James C., Thomas, Pamela J., McDowell, Jennifer C., Maskeri, Baishali, ...
Although the cost of generating draft-quality genomic sequence continues to decline, refining that sequence by the process of “sequence finishing” remains expensive. Near-perfect finished...
An intermediate grade of finished genomic sequence suitable for comparative analyses (2004)
Blakesley, Robert W., Hansen, Nancy F., Mullikin, James C., Thomas, Pamela J., McDowell, Jennifer C., Maskeri, Baishali, ...
Although the cost of generating draft-quality genomic sequence continues to decline, refining that sequence by the process of “sequence finishing” remains expensive. Near-perfect finished...
Pericentromeric Duplications in the Laboratory Mouse (2003)
Thomas, James W., Schueler, Mary G., Summers, Tyrone J., Blakesley, Robert W., McDowell, Jennifer C., Thomas, Pamela J., ...
Duplications have long been postulated to be an important mechanism by which genomes evolve. Interspecies genomic comparisons are one method by which the origin and molecular mechanism of...
Pericentromeric Duplications in the Laboratory Mouse (2002)
Thomas, James W., Schueler, Mary G., Summers, Tyrone J., Blakesley, Robert W., McDowell, Jennifer C., Thomas, Pamela J., ...
Systematic sequencing of cDNA clones using the transposon Tn5 (2002)
Shevchenko, Yuriy, Bouffard, Gerard G., Butterfield, Yaron S. N., Blakesley, Robert W., Hartley, James L., Young, Alice C., ...
In parallel with the production of genomic sequence data, attention is being focused on the generation of comprehensive cDNA-sequence resources. Such efforts are increasingly emphasizing the...
DeSilva, Udaya, Elnitski, Laura, Idol, Jacquelyn R., Doyle, Johannah L., Gan, Weiniu, Thomas, James W., ...
Genetic organization of the KpnI restriction -- modification system (1991)
Chatterjee, Deb K., Hammond, Alan W., Blakesley, Robert W., Adams, Shelia M., Gerard, Gary F.
The KpnI restriction-modification (KpnI RM) system was previously cloned and expressed in E. coli. The nucleotide sequences of the Kpnl endonuclease (R. KpnI) and methylase (M. KpnI) genes have now...
Linear amplification DNA sequencing directly from single phage plaques and bacterial colonies (1991)
Krishnan, B.Rajendra, Blakesley, Robert W., Berg, Douglas E.
RSITE: a computer program to predict the recognition sequence of a restriction enzyme (1982)
Tolstoshev, Carolyn M., Blakesley, Robert W.
A computer program (RSITE) was developed which predicts the recognition sequence of a restriction endonuclease. The sizes of fragments experimentally determined on cleavage of a DNA of known sequence...
Antibiotic induced ekctrophoretic mobility shifts of DNA restriction fragments (1979)
Loucks, Ellen, Chaconas, George, Blakesley, Robert W., Wells, Robert D., Van De Sande, Johan H.
Several antibiotics , netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been found to alter the...
Preparation and properties of insolubilized restriction endonucleases (1978)
Lee, Yan-Hwa, Blakesley, Robert W., Smith, Leonard A., Chirikjian, Jack G.
Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by...
Systematic sequencing of cDNA clones using the transposon Tn5
Shevchenko, Yuriy, Bouffard, Gerard G., Butterfield, Yaron S. N., Blakesley, Robert W., Hartley, James L., Young, Alice C., ...
In parallel with the production of genomic sequence data, attention is being focused on the generation of comprehensive cDNA-sequence resources. Such efforts are increasingly emphasizing the...
Marek's Disease Herpesvirus-Induced DNA Polymerase
Boezi, John A., Lee, Lucy F., Blakesley, Robert W., Koenig, Mark, Towle, Howard C.
Infection of duck embryo fibroblasts by Marek's disease herpesvirus (MDHV), strain GA, led to the induction of a novel DNA polymerase. This novel DNA polymerase, designated MDHV-induced DNA...
Pericentromeric Duplications in the Laboratory Mouse
Thomas, James W., Schueler, Mary G., Summers, Tyrone J., Blakesley, Robert W., McDowell, Jennifer C., Thomas, Pamela J., ...
Duplications have long been postulated to be an important mechanism by which genomes evolve. Interspecies genomic comparisons are one method by which the origin and molecular mechanism of...
An intermediate grade of finished genomic sequence suitable for comparative analyses
Blakesley, Robert W., Hansen, Nancy F., Mullikin, James C., Thomas, Pamela J., McDowell, Jennifer C., Maskeri, Baishali, ...
Although the cost of generating draft-quality genomic sequence continues to decline, refining that sequence by the process of “sequence finishing” remains expensive. Near-perfect finished...
DeSilva, Udaya, Elnitski, Laura, Idol, Jacquelyn R., Doyle, Johannah L., Gan, Weiniu, Thomas, James W., ...
Williams syndrome is a complex developmental disorder that results from the heterozygous deletion of a ∼1.6-Mb segment of human chromosome 7q11.23. These deletions are mediated by large (∼300 kb)...
Morin, Ryan D., Chang, Elbert, Petrescu, Anca, Liao, Nancy, Griffith, Malachi, Kirkpatrick, Robert, ...
Sequencing of full-insert clones from full-length cDNA libraries from both Xenopus laevis and Xenopus tropicalis has been ongoing as part of the Xenopus Gene Collection Initiative. Here we present...
Systematic sequencing of cDNA clones using the transposon Tn5
Shevchenko, Yuriy, Bouffard, Gerard G., Butterfield, Yaron S. N., Blakesley, Robert W., Hartley, James L., Young, Alice C., ...
In parallel with the production of genomic sequence data, attention is being focused on the generation of comprehensive cDNA-sequence resources. Such efforts are increasingly emphasizing the...
DeSilva, Udaya, Elnitski, Laura, Idol, Jacquelyn R., Doyle, Johannah L., Gan, Weiniu, Thomas, James W., ...
Williams syndrome is a complex developmental disorder that results from the heterozygous deletion of a ∼1.6-Mb segment of human chromosome 7q11.23. These deletions are mediated by large (∼300 kb)...
Marek's Disease Herpesvirus-Induced DNA Polymerase
Boezi, John A., Lee, Lucy F., Blakesley, Robert W., Koenig, Mark, Towle, Howard C.
Infection of duck embryo fibroblasts by Marek's disease herpesvirus (MDHV), strain GA, led to the induction of a novel DNA polymerase. This novel DNA polymerase, designated MDHV-induced DNA...
Pericentromeric Duplications in the Laboratory Mouse
Thomas, James W., Schueler, Mary G., Summers, Tyrone J., Blakesley, Robert W., McDowell, Jennifer C., Thomas, Pamela J., ...
Duplications have long been postulated to be an important mechanism by which genomes evolve. Interspecies genomic comparisons are one method by which the origin and molecular mechanism of...
An intermediate grade of finished genomic sequence suitable for comparative analyses
Blakesley, Robert W., Hansen, Nancy F., Mullikin, James C., Thomas, Pamela J., McDowell, Jennifer C., Maskeri, Baishali, ...
Although the cost of generating draft-quality genomic sequence continues to decline, refining that sequence by the process of “sequence finishing” remains expensive. Near-perfect finished...
Morin, Ryan D., Chang, Elbert, Petrescu, Anca, Liao, Nancy, Griffith, Malachi, Kirkpatrick, Robert, ...
Sequencing of full-insert clones from full-length cDNA libraries from both Xenopus laevis and Xenopus tropicalis has been ongoing as part of the Xenopus Gene Collection Initiative. Here we present...
Analyses of deep mammalian sequence alignments and constraint predictions for 1% of the human genome
Margulies, Elliott H., Cooper, Gregory M., Asimenos, George, Thomas, Daryl J., Dewey, Colin N., Siepel, Adam, ...
A key component of the ongoing ENCODE project involves rigorous comparative sequence analyses for the initially targeted 1% of the human genome. Here, we present orthologous sequence generation,...
A diversity profile of the human skin microbiota
Grice, Elizabeth A., Kong, Heidi H., Renaud, Gabriel, Young, Alice C., Bouffard, Gerard G., Blakesley, Robert W., ...
The many layers and structures of the skin serve as elaborate hosts to microbes, including a diversity of commensal and pathogenic bacteria that contribute to both human health and disease. To...