Roberto P. Werkhauser

Development of a real time polymerase chain reaction for quantitation of Schistosoma mansoni   DNA (2007)

Ana Lisa Do Vale Gomes, Fábio L Melo, Roberto P Werkhauser, Frederico GC Abath

This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument. Primers targeting the gene encoding...

Molecular approaches for the detection of Schistosoma mansoni   : possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection (2007)

Frederico GC Abath, Ana Lisa Do Vale Gomes, Fábio L Melo, Constança S Barbosa, Roberto P Werkhauser

The detection of specific DNA sequences by polymerase chain reaction (PCR) has proved extremely valuable for the analysis of genetic disorders and the diagnosis of a variety of infectious disease...

Development of a real time polymerase chain reaction for quantitation of Schistosoma mansoni DNA (2006)

Gomes,Ana Lisa Do Vale, Melo,Fábio L, Werkhauser,Roberto P, Abath,Frederico GC

This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument. Primers targeting the gene encoding...

Molecular approaches for the detection of Schistosoma mansoni: possible applications in the detection of snail infection, monitoring of transmission sites, and diagnosis of human infection (2006)

Abath,Frederico GC, Gomes,Ana Lisa Do Vale, Melo,Fábio L, Barbosa,Constança S, Werkhauser,Roberto P

The detection of specific DNA sequences by polymerase chain reaction (PCR) has proved extremely valuable for the analysis of genetic disorders and the diagnosis of a variety of infectious disease...

SHORT COMMUNICATION - Partial Molecular Characterization of Sm8, a Tegumental Antigen of Schistosoma mansoni   (2002)

Frederico GC Abath, Edeneide M Xavier, Sílvia ML Montenegro, Roberto P Werkhauser

Sm8 is a major tegumental antigen of Schistosoma mansoni   . The partial cDNA was isolated and analyzed. Sequence analysis revealed transmembrane compatible hydrophobic domains and a putative...

Partial molecular characterization of Sm8, a tegumental antigen of Schistosoma mansoni (2002)

Abath,Frederico GC, Xavier,Edeneide M, Montenegro,Sílvia ML, Werkhauser,Roberto P

Sm8 is a major tegumental antigen of Schistosoma mansoni. The partial cDNA was isolated and analyzed. Sequence analysis revealed transmembrane compatible hydrophobic domains and a putative leucine...

RESEARCH NOTE - The Tegument of Schistosoma mansoni: Genes, Antigens and the Host-Parasite Relationship (1998)

Edneide M Xavier, Norma Lucena-Silva, Roberto P Werkhauser, Gloria R Franco, Ricardo AA Lira Santos, Andrew JG Simpson, ...

Cercaria, the infective larvae of Schistosoma mansoni are released from a molluscan host and must penetrate the skin of the human host within several hours in order to continue the life-cycle of the...