Rodney L. Levine

Transcriptional profiling of MnSOD-mediated lifespan extension in Drosophilareveals a species-general network of aging and metabolic genes (2007)

Curtis, Christina, Landis, Gary N, Folk, Donna, Wehr, Nancy B, Hoe, Nicholas, Waskar, Morris, ...

Abstract Background Several interventions increase lifespan in model organisms, including reduced insulin/insulin-like growth factor-like signaling (IIS), FOXO transcription factor activation,...

Loss of the ataxia-telangiectasia gene product causes oxidative damage in target organs. (1999)

Barlow, Carrolee, Dennery, Phyllis A., Shigenaga, Mark K., Smith, Mark A., Morrow, Jason D., Roberts, L. Jackson, ...

Ataxia-telangiectasia (A-T) is characterized by a markedly increased sensitivity to ionizing radiation, increased incidence of cancer, and neurodegeneration, especially of the cerebellar Purkinje...

Glutamic and aminoadipic semialdehydes are the main carbonyl products of metal-catalyzed oxidation of proteins

Requena, Jesús R., Chao, Chien-Chung, Levine, Rodney L., Stadtman, Earl R.

Metal-catalyzed oxidation results in loss of function and structural alteration of proteins. The oxidative process affects a variety of side amino acid groups, some of which are converted to carbonyl...

Antioxidant activity of Ferrozine–iron–amino acid complexes

Berlett, Barbara S., Levine, Rodney L., Chock, P. Boon, Chevion, Mordechai, Stadtman, Earl R.

Amino acid–Fe(II)–chelator complexes exhibit strong antioxidant activity. Taking advantage of the unique spectral characteristics of the complexes formed when Ferrozine (Fz) is used as the...

Mammalian thioredoxin reductase: Oxidation of the C-terminal cysteine/selenocysteine active site forms a thioselenide, and replacement of selenium with sulfur markedly reduces catalytic activity

Lee, Seung-Rock, Bar-Noy, Shoshana, Kwon, Jaeyul, Levine, Rodney L., Stadtman, Thressa C., Rhee, Sue Goo

Mammalian cytosolic thioredoxin reductase (TrxR) has a redox center, consisting of Cys59/Cys64 adjacent to the flavin ring of FAD and another center consisting of Cys497/selenocysteine (SeCys)498...

Carbon dioxide stimulates peroxynitrite-mediated nitration of tyrosine residues and inhibits oxidation of methionine residues of glutamine synthetase: Both modifications mimic effects of adenylylation

Berlett, Barbara S., Levine, Rodney L., Stadtman, Earl R.

The activity of glutamine synthetase (EC 6.3.1.2) from Escherichia coli is regulated by the cyclic adenylylation and deadenylylation of Tyr-397 in each of the enzyme’s 12 identical subunits. The...

Iron-dependent oxidation, ubiquitination, and degradation of iron regulatory protein 2: Implications for degradation of oxidized proteins

Iwai, Kazuhiro, Drake, Steven K., Wehr, Nancy B., Weissman, Allan M., LaVaute, Timothy, Minato, Nagahiro, ...

The ability of iron to catalyze formation of reactive oxygen species significantly contributes to its toxicity in cells and animals. Iron uptake and distribution is regulated tightly in mammalian...

Peroxynitrite-mediated modification of proteins at physiological carbon dioxide concentration: pH dependence of carbonyl formation, tyrosine nitration, and methionine oxidation

Tien, Ming, Berlett, Barbara S., Levine, Rodney L., Chock, P. Boon, Stadtman, Earl R.

The ability of peroxynitrite to modify amino acid residues in glutamine synthetase (GS) and BSA is greatly influenced by pH and CO2. At physiological concentrations of CO2 (1.3 mM), the generation of...

Loss of the ataxia–telangiectasia gene product causes oxidative damage in target organs

Barlow, Carrolee, Dennery, Phyllis A., Shigenaga, Mark K., Smith, Mark A., Morrow, Jason D., Roberts, L. Jackson, ...

Ataxia–telangiectasia (A-T) is characterized by a markedly increased sensitivity to ionizing radiation, increased incidence of cancer, and neurodegeneration, especially of the cerebellar Purkinje...

Oxidative damage during aging targets mitochondrial aconitase

Yan, Liang-Jun, Levine, Rodney L., Sohal, Rajindar S.

The mechanisms that cause aging are not well understood. The oxidative stress hypothesis proposes that the changes associated with aging are a consequence of random oxidative damage to biomolecules....

Copper-catalyzed oxidation of the recombinant SHa(29–231) prion protein

Requena, Jesús R., Groth, Darlene, Legname, Giuseppe, Stadtman, Earl R., Prusiner, Stanley B., Levine, Rodney L.

Metal-catalyzed oxidation may result in structural damage to proteins and has been implicated in aging and disease, including neurological disorders such as Alzheimer's disease and amyotrophic...

ADP ribosylation of human neutrophil peptide-1 regulates its biological properties

Paone, Gregorino, Wada, Akihiro, Stevens, Linda A., Matin, Abul, Hirayama, Toshiya, Levine, Rodney L., ...

In human airways, epithelial cells lining the lumen and intraluminal cells (e.g., polymorphonuclear cells) participate in the innate immune response. These cells secrete or express on their surfaces...

Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins

Lacourciere, Gerard M., Levine, Rodney L., Stadtman, Thressa C.

Selenium can be metabolized for protein synthesis by two major pathways in vivo. In a specific pathway it can be inserted into polypeptide chains as the amino acid selenocysteine, as directed by the...

Group B streptococcal phospholipid causes pulmonary hypertension

Curtis, Jerri, Kim, Geumsoo, Wehr, Nancy B., Levine, Rodney L.

Group B Streptococcus is the most common cause of bacterial infection in the newborn. Infection in many cases causes persistent pulmonary hypertension, which impairs gas exchange in the lung. We...

High urea and NaCl carbonylate proteins in renal cells in culture and in vivo, and high urea causes 8-oxoguanine lesions in their DNA

Zhang, Zheng, Dmitrieva, Natalia I., Park, Jong-Hwan, Levine, Rodney L., Burg, Maurice B.

Urea and NaCl are elevated in the renal inner medulla. We now find that a high concentration of urea or NaCl increases reactive oxygen species (ROS) in mouse renal inner medullary (mIMCD3) cells in...

Carbonic Anhydrase III Is Not Required in the Mouse for Normal Growth, Development, and Life Span

Kim, Geumsoo, Lee, Tae-Hoon, Wetzel, Petra, Geers, Cornelia, Robinson, Mary Ann, Myers, Timothy G., ...

Carbonic anhydrase III is a cytosolic protein which is particularly abundant in skeletal muscle, adipocytes, and liver. The specific activity of this isozyme is quite low, suggesting that its...

Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence

Zhao, Hongtao, Joseph, Joy, Fales, Henry M., Sokoloski, Edward A., Levine, Rodney L., Vasquez-Vivar, Jeannette, ...

Here we report the structural characterization of the product formed from the reaction between hydroethidine (HE) and superoxide (\documentclass[12pt]{minimal} \usepackage{amsmath}...

Glutamic and aminoadipic semialdehydes are the main carbonyl products of metal-catalyzed oxidation of proteins

Requena, Jesús R., Chao, Chien-Chung, Levine, Rodney L., Stadtman, Earl R.

Metal-catalyzed oxidation results in loss of function and structural alteration of proteins. The oxidative process affects a variety of side amino acid groups, some of which are converted to carbonyl...

Antioxidant activity of Ferrozine–iron–amino acid complexes

Berlett, Barbara S., Levine, Rodney L., Chock, P. Boon, Chevion, Mordechai, Stadtman, Earl R.

Amino acid–Fe(II)–chelator complexes exhibit strong antioxidant activity. Taking advantage of the unique spectral characteristics of the complexes formed when Ferrozine (Fz) is used as the...

Mammalian thioredoxin reductase: Oxidation of the C-terminal cysteine/selenocysteine active site forms a thioselenide, and replacement of selenium with sulfur markedly reduces catalytic activity

Lee, Seung-Rock, Bar-Noy, Shoshana, Kwon, Jaeyul, Levine, Rodney L., Stadtman, Thressa C., Rhee, Sue Goo

Mammalian cytosolic thioredoxin reductase (TrxR) has a redox center, consisting of Cys59/Cys64 adjacent to the flavin ring of FAD and another center consisting of Cys497/selenocysteine (SeCys)498...

Carbon dioxide stimulates peroxynitrite-mediated nitration of tyrosine residues and inhibits oxidation of methionine residues of glutamine synthetase: Both modifications mimic effects of adenylylation

Berlett, Barbara S., Levine, Rodney L., Stadtman, Earl R.

The activity of glutamine synthetase (EC 6.3.1.2) from Escherichia coli is regulated by the cyclic adenylylation and deadenylylation of Tyr-397 in each of the enzyme’s 12 identical subunits. The...

Iron-dependent oxidation, ubiquitination, and degradation of iron regulatory protein 2: Implications for degradation of oxidized proteins

Iwai, Kazuhiro, Drake, Steven K., Wehr, Nancy B., Weissman, Allan M., LaVaute, Timothy, Minato, Nagahiro, ...

The ability of iron to catalyze formation of reactive oxygen species significantly contributes to its toxicity in cells and animals. Iron uptake and distribution is regulated tightly in mammalian...

Peroxynitrite-mediated modification of proteins at physiological carbon dioxide concentration: pH dependence of carbonyl formation, tyrosine nitration, and methionine oxidation

Tien, Ming, Berlett, Barbara S., Levine, Rodney L., Chock, P. Boon, Stadtman, Earl R.

The ability of peroxynitrite to modify amino acid residues in glutamine synthetase (GS) and BSA is greatly influenced by pH and CO2. At physiological concentrations of CO2 (1.3 mM), the generation of...

Loss of the ataxia–telangiectasia gene product causes oxidative damage in target organs

Barlow, Carrolee, Dennery, Phyllis A., Shigenaga, Mark K., Smith, Mark A., Morrow, Jason D., Roberts, L. Jackson, ...

Ataxia–telangiectasia (A-T) is characterized by a markedly increased sensitivity to ionizing radiation, increased incidence of cancer, and neurodegeneration, especially of the cerebellar Purkinje...

Oxidative damage during aging targets mitochondrial aconitase

Yan, Liang-Jun, Levine, Rodney L., Sohal, Rajindar S.

The mechanisms that cause aging are not well understood. The oxidative stress hypothesis proposes that the changes associated with aging are a consequence of random oxidative damage to biomolecules....

Copper-catalyzed oxidation of the recombinant SHa(29–231) prion protein

Requena, Jesús R., Groth, Darlene, Legname, Giuseppe, Stadtman, Earl R., Prusiner, Stanley B., Levine, Rodney L.

Metal-catalyzed oxidation may result in structural damage to proteins and has been implicated in aging and disease, including neurological disorders such as Alzheimer's disease and amyotrophic...

ADP ribosylation of human neutrophil peptide-1 regulates its biological properties

Paone, Gregorino, Wada, Akihiro, Stevens, Linda A., Matin, Abul, Hirayama, Toshiya, Levine, Rodney L., ...

In human airways, epithelial cells lining the lumen and intraluminal cells (e.g., polymorphonuclear cells) participate in the innate immune response. These cells secrete or express on their surfaces...

Direct detection of potential selenium delivery proteins by using an Escherichia coli strain unable to incorporate selenium from selenite into proteins

Lacourciere, Gerard M., Levine, Rodney L., Stadtman, Thressa C.

Selenium can be metabolized for protein synthesis by two major pathways in vivo. In a specific pathway it can be inserted into polypeptide chains as the amino acid selenocysteine, as directed by the...

Group B streptococcal phospholipid causes pulmonary hypertension

Curtis, Jerri, Kim, Geumsoo, Wehr, Nancy B., Levine, Rodney L.

Group B Streptococcus is the most common cause of bacterial infection in the newborn. Infection in many cases causes persistent pulmonary hypertension, which impairs gas exchange in the lung. We...

High urea and NaCl carbonylate proteins in renal cells in culture and in vivo, and high urea causes 8-oxoguanine lesions in their DNA

Zhang, Zheng, Dmitrieva, Natalia I., Park, Jong-Hwan, Levine, Rodney L., Burg, Maurice B.

Urea and NaCl are elevated in the renal inner medulla. We now find that a high concentration of urea or NaCl increases reactive oxygen species (ROS) in mouse renal inner medullary (mIMCD3) cells in...

Carbonic Anhydrase III Is Not Required in the Mouse for Normal Growth, Development, and Life Span

Kim, Geumsoo, Lee, Tae-Hoon, Wetzel, Petra, Geers, Cornelia, Robinson, Mary Ann, Myers, Timothy G., ...

Carbonic anhydrase III is a cytosolic protein which is particularly abundant in skeletal muscle, adipocytes, and liver. The specific activity of this isozyme is quite low, suggesting that its...

Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence

Zhao, Hongtao, Joseph, Joy, Fales, Henry M., Sokoloski, Edward A., Levine, Rodney L., Vasquez-Vivar, Jeannette, ...

Here we report the structural characterization of the product formed from the reaction between hydroethidine (HE) and superoxide (\documentclass[12pt]{minimal} \usepackage{amsmath}...

Phosphorylation of actin Tyr-53 inhibits filament nucleation and elongation and destabilizes filaments

Liu, Xiong, Shu, Shi, Hong, Myoung-Soon S., Levine, Rodney L., Korn, Edward D.

Dictyostelium actin was shown to become phosphorylated on Tyr-53 late in the developmental cycle and when cells in the amoeboid stage are subjected to stress but the phosphorylated actin had not been...

Transcriptional profiling of MnSOD-mediated lifespan extension in Drosophila reveals a species-general network of aging and metabolic genes

Curtis, Christina, Landis, Gary N, Folk, Donna, Wehr, Nancy B, Hoe, Nicholas, Waskar, Morris, ...

Transcriptional profiling of MnSOD-mediated life-span extension in Drosophila identifies a set of candidate biomarkers of aging, consisting primarily of carbohydrate metabolism and electron transport...

Modification of Cysteine Residues In Vitro and In Vivo Affects the Immunogenicity and Antigenicity of Major Histocompatibility Complex Class I–restricted Viral Determinants

Chen, Weisan, Yewdell, Jonathan W., Levine, Rodney L., Bennink, Jack R.

In studying the subdominant status of two cysteine-containing influenza virus nuclear protein (NP) determinants (NP39–47 and NP218–226) restricted by H-2Kd, we found that the antigenicity of...

Elafin, an Elastase-specific Inhibitor, Is Cleaved by Its Cognate Enzyme Neutrophil Elastase in Sputum from Individuals with Cystic Fibrosis*

Guyot, Nicolas, Butler, Marcus W., McNally, Paul, Weldon, Sinead, Greene, Catherine M., Levine, Rodney L., ...

Elafin is a neutrophil serine protease inhibitor expressed in lung and displaying anti-inflammatory and anti-bacterial properties. Previous studies demonstrated that some innate host defense...