Roel A. L. Bovenberg

Characterization of a phenylacetate–CoA ligase from Penicillium chrysogenum (2009)

Janssen, Dick B., Bovenberg, Roel A.L., Jekel, Peter A., Koetsier, Martijn J.

Enzymatic activation of PAA (phenylacetic acid) to phenylacetyl-CoA is an important step in the biosynthesis of the β-lactam antibiotic penicillin G by the fungus Penicillium chrysogenum. CoA esters...

Matching the proteome to the genome: the microbody of penicillin-producing Penicillium chrysogenum cells (2009)

Fusetti, Fabrizia, Poolman, Bert, Bovenberg, Roel A.L., Veenhuis, Marten, ...

In the filamentous fungus Penicillium chrysogenum, microbodies are essential for penicillin biosynthesis. To better understand the role of these organelles in antibiotics production, we determined...

Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha (2008)

Gidijala, Loknath, Bovenberg, Roel A.L., Klaassen, Paul, Veenhuis, Marten

Background: β-Lactams like penicillin and cephalosporin are among the oldest known antibiotics used against bacterial infections. Industrially, penicillin is produced by the filamentous fungus...

Expression of the transporter encoded by the cefT gene of Acremonium chrysogenum increases cephalosporin production in Penicillium chrysogenum (2008)

Nijland, Jeroen G., Kovalchuk, Andriy, Bovenberg, Roel A.L., Driessen, Arnold J.M.

By introduction of the cefEF genes of Acremonium chrysogenum and the cmcH gene of Streptomyces clavuligerus, Penicillium chrysogenum can be reprogrammed to form...

Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum (2008)

Albang, Richard, Albermann, Kaj, Badger, Jonathan H., Daran, Jean-Marc, Driessen, Arnold J.M., ...

Industrial penicillin production with the filamentous fungus Penicillium chrysogenum is based on an unprecedented effort in microbial strain improvement. To gain more insight into penicillin...

Synthesis of Penicillium chrysogenum acetyl-CoA:isopenicillin N acyltransferase in Hansenula polymorpha: First step towards the introduction of a new metabolic pathway (2005)

Lutz, Marco V., Bovenberg, Roel A.L., Veenhuis, Marten

The enzyme acetyl-CoA:isopenicillin N acyltransferase (IAT) is a peroxisomal enzyme that mediates the final step of penicillin biosynthesis in the filamentous fungi Penicillium chrysogenum and...

Overproduction of a single protein, Pc-Pex11p, results in 2-fold enhanced penicillin production by Penicillium chrysogenum (2005)

Bovenberg, Roel A.L., Veenhuis, Marten

Current industrial production of β-lactam antibiotics, using the filamentous fungus Penicillium chrysogenum, is the result of many years of strain improvement by classical mutagenesis. More effcient...

Penicillium chrysogenum Pex5p mediates differential sorting of PTS1 proteins to microbodies of the methylotrophic yeast Hansenula polymorpha (2004)

Berg, Marco Van Den, Bovenberg, Roel A.L., Veenhuis, Marten

We have isolated the Penicillium chrysogenum pex5 gene encoding the receptor for microbody matrix proteins containing a type 1 peroxisomal targeting signal (PTS1). Pc-pex5 contains 2 introns and...

δ-(L-α-Aminoadipyl)-L-cysteinyl-D-valine synthetase, that mediates the first committed step in penicillin biosynthesis, is a cytosolic enzyme (2002)

Kamp, Mart Van De, Berg, Marco Van Den, Sjollema, Klaas, Bovenberg, Roel A.L., Veenhuis, Marten, ...

Penicillin biosynthesis by Penicillium chrysogenum is a compartmentalized process. The first catalytic step is mediated by δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACV synthetase), a...