Rolf Müller

Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermopilic isolate Corallococcus macrosporusGT-2 (2009)

Perlova, Olena, Gerth, Klaus, Kuhlmann, Silvia, Zhang, Youming, Müller, Rolf

Abstract Although many secondary metabolites with diverse biological activities have been isolated from myxobacteria, most strains of these biotechnologically important gliding prokaryotes remain...

HP: GeneTrail – advanced gene set enrichment analysis (2008)

Christina Backes, Andreas Keller, Jan Kuentzer, Benny Kneissl, Nicole Comtesse, Yasser A. Elnakady, ...

We present a comprehensive and efficient gene set analysis tool, called ‘GeneTrail ’ that offers a rich functionality and is easy to use. Our web-based application facilitates the statistical...

HP: GeneTrail – advanced gene set enrichment analysis (2008)

Christina Backes, Andreas Keller, Jan Kuentzer, Benny Kneissl, Nicole Comtesse, Yasser A. Elnakady, ...

We present a comprehensive and efficient gene set analysis tool, called ‘GeneTrail ’ that offers a rich functionality and is easy to use. Our web-based application facilitates the statistical...

MULTIDIMENSIONAL PEPTIDE/PROTEIN ANALYSIS AND IDENTIFICATION BY SEQUENCE DATABASE SEARCH USING MASS SPECTROMETRIC DATA (2008)

Christian Schley, Matthias Altmeyer, Rolf Müller, Christian G. Huber

Abstract. In order to generate proteomics data that are suitable to validate protein identification in complex mixtures using multidimensional liquidchromatography-mass spectrometry approaches, we...

Simple measures of ozone depletion in the polar stratosphere (2008)

Müller, Rolf, Grooß, Jens-Uwe, Lemmen, Carsten, Heinze, D., Dameris, Martin, Bodeker, Greg

We investigate the extent to which quantities that are based on total column ozone are applicable as measures of ozone loss in the polar vortices. Such quantities have been used frequently in ozone...

A brief history of stratospheric ozone research (2008)

Müller, Rolf

Abstract Ozone is one of the most important trace species in the atmosphere. Therefore, the history of research on ozone has also received a good deal of attention. Here a short overview of ozone...

Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition (2008)

Fu, Jun, Wenzel, Silke C., Perlova, Olena, Wang, Junping, Gross, Frank, Tang, Zhiru, ...

Horizontal gene transfer by transposition has been widely used for transgenesis in prokaryotes. However, conjugation has been preferred for transfer of large transgenes, despite greater restrictions...

A Role for PPARβ/δ in Tumor Stroma and Tumorigenesis (2008)

Rolf Müller, Martin Kömhoff, Jeffrey M. Peters, Sabine Müller-Brüsselbach

Peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) is a transcription factor that is activated by endogenous fatty acid ligands and by synthetic agonists. Its role in the regulation of...

Regulation of Cell Proliferation and Differentiation by PPARβ/δ (2008)

Rolf Müller, Markus Rieck, Sabine Müller-Brüsselbach

Peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) is a ligand-activated transcription factor with essential functions in the regulation of lipid catabolism, glucose homeostasis, and...

GeneTrail--advanced gene set enrichment analysis (2007)

Backes, Christina, Keller, Andreas, Kuentzer, Jan, Kneissl, Benny, Comtesse, Nicole, Elnakady, Yasser A., ...

We present a comprehensive and efficient gene set analysis tool, called ‘GeneTrail’ that offers a rich functionality and is easy to use. Our web-based application facilitates the statistical...

GrlJ, a DictyosteliumGABAB-like receptor with roles in post-aggregation development (2007)

Prabhu, Yogikala, Müller, Rolf, Anjard, Christophe, Noegel, Angelika A

Abstract Background The G-protein-coupled receptor (GPCR) family represents the largest and most important group of targets for chemotherapeutics. They are extremely versatile receptors that...

Critique of the tracer-tracer correlation technique and its potential to analyze polar ozone loss in chemistry-climate models (2006)

Lemmen, Carsten, Müller, Rolf, Konopka, Paul, Dameris, Martin

The tracer-tracer correlation technique (TRAC) has been widely employed to infer chemical ozone loss from observations. Yet, its applicability to chemistry-climate model (CCM) data is disputed. Here,...

Chemical ozone loss in the Arctic and Antarctic stratosphere (2006)

Tilmes, Simone, Müller, Rolf, Engel, Andreas, Rex, Markus, Russell III, James M.

An edited version of this paper was published by AGU. Copyright 2001 American Geophysical Union. To view the published open abstract, go to http://dx.doi.org/10.1029/2006GL026925

Chemical ozone loss in a chemistry-climate model from 1960-1999 (2006)

Lemmen, Carsten, Dameris, Martin, Müller, Rolf, Riese, Martin

In the recent WMO assessment of ozone depletion, the minimum ozone column is used to assess the evolution of the polar ozone layer simulated in several chemistry-climate models (CCMs). The ozone...

Chemical ozone loss and related processes in the Antarctic winter 2003 based on Improved Limb Atmospheric Spectrometer (ILAS)–II observations (2006)

Tilmes, Simone, Müller, Rolf, Grooß, Jens-Uwe, Spang, Reinhold, Sugita, Takafumi, Nakajima, Hideaki, ...

An edited version of this paper was published by AGU. Copyright 2006 American Geophysical Union. To view the published open abstract, go to http://dx.doi.org/10.1029/2005JD006260

Development of tracer relations and chemical ozone loss (2006)

Tilmes, Simone, Müller, Rolf, Grooß, Jens-Uwe, Nakajima, Hideaki, Sasano, Yasuhiro

An edited version of this paper was published by AGU. Copyright 2006 American Geophysical Union. To view the published open abstract, go to http://dx.doi.org/10.1029/2005JD006726 .

Soziales Lernen als Baustein des Schulprogramms. Gymnasium Horn-Bad Meinberg, Nordrhein-Westfalen (2006)

Müller, Rolf

Im Mittelpunkt des Konzeptes "Soziales Lernen" am Gymnasium Horn-Bad Meinberg stehen der Ausbau der sozialen Kompetenzen der Schüler/-innen, die Stärkung ihrer Selbstwahrnehmung und die Förderung...

MULTIDIMENSIONAL PEPTIDE/PROTEIN ANALYSIS AND IDENTIFICATION BY SEQUENCE DATABASE SEARCH USING MASS SPECTROMETRIC DATA (2006)

Schley, Christian, Altmeyer, Matthias, Müller, Rolf, Huber, Christian G.

In order to generate proteomics data that are suitable to validate protein identification in complex mixtures using multidimensional liquid-chromatography-mass spectrometry approaches, we implemented...

The Dictyosteliumgenome encodes numerous RasGEFs with multiple biological roles (2005)

Wilkins, Andrew, Szafranski, Karol, Fraser, Derek J, Bakthavatsalam, Deenadayalan, Müller, Rolf, Fisher, Paul R, ...

Abstract Background Dictyostelium discoideum is a eukaryote with a simple lifestyle and a relatively small genome whose sequence has been fully determined. It is widely used for studies on cell...

Knowledge Mining for Biomimetic Smart Antenna Shapes. Robotics and Autonomous Systems (2005)

Rolf Müller

Antennas used on autonomous robots have been optimized very little for the tasks which need to be mastered in order to achieve true autonomy. In contrast to this, natural biosonar systems offer a...

Evolutionary Implications of Bacterial Polyketide Synthases (2005)

Jenke-Kodama, Holger, Sandmann, Axel, Müller, Rolf, Dittmann, Elke

Polyketide synthases (PKS) perform a stepwise biosynthesis of diverse carbon skeletons from simple activated carboxylic acid units. The products of the complex pathways possess a wide range of...

Evolutionary Implications of Bacterial Polyketide Synthases (2005)

Jenke-Kodama, Holger, Sandmann, Axel, Müller, Rolf, Dittmann, Elke

Polyketide synthases (PKS) perform a stepwise biosynthesis of diverse carbon skeletons from simple activated carboxylic acid units. The products of the complex pathways possess a wide range of...

Evolutionary Implications of Bacterial Polyketide Synthases (2005)

Jenke-Kodama, Holger, Sandmann, Axel, Müller, Rolf, Dittmann, Elke

Polyketide synthases (PKS) perform a stepwise biosynthesis of diverse carbon skeletons from simple activated carboxylic acid units. The products of the complex pathways possess a wide range of...

Mixing and ozone loss in the 1999–2000 Arctic vortex: Simulations with the three-dimensional Chemical Lagrangian Model of the Stratosphere (CLaMS) (2004)

Konopka, Paul, Steinhorst, Hildegard-Maria, Grooß, Jens-Uwe, Günther, Gebhard, Müller, Rolf, Elkins, James W., ...

To view the published open abstract, go to http://dx.doi.org/10.1029/2003JD003792 . An edited version of this paper was published by AGU. Copyright 2004 American Geophysical Union.

Mixing and ozone loss in the 1999–2000 Arctic vortex: Simulations with the three-dimensional Chemical Lagrangian Model of the Stratosphere (CLaMS) (2004)

Konopka, Paul, Steinhorst, Hildegard-Maria, Grooß, Jens-Uwe, Günther, Gebhard, Müller, Rolf, Elkins, James W., ...

To view the published open abstract, go to http://dx.doi.org/10.1029/2003JD003792 An edited version of this paper was published by AGU. Copyright 2004 American Geophysical Union.

Novel RGD lipopeptides for the targeting of liposomes to integrin-expressing endothelial and melanoma cells (2004)

Hölig, Peter, Bach, Miriam, Völkel, Tina, Nahde, Thomas, Hoffmann, Sven, Müller, Rolf, ...

RGD peptides targeting αv-integrins are promising ligands for the generation of vascular targeting agents. We isolated from phage display RGD motif libraries novel high-affinity cyclic RGD peptides...

Novel RGD lipopeptides for the targeting of liposomes to integrin-expressing endothelial and melanoma cells (2004)

Hölig, Peter, Bach, Miriam, Völkel, Tina, Nahde, Thomas, Hoffmann, Sven, Müller, Rolf, ...

RGD peptides targeting αv-integrins are promising ligands for the generation of vascular targeting agents. We isolated from phage display RGD motif libraries novel high affinity cyclic RGD peptides...

Novel RGD lipopeptides for the targeting of liposomes to integrin-expressing endothelial and melanoma cells (2004)

Hölig, Peter, Bach, Miriam, Völkel, Tina, Nahde, Thomas, Hoffmann, Sven, Müller, Rolf, ...

RGD peptides targeting αv-integrins are promising ligands for the generation of vascular targeting agents. We isolated from phage display RGD motif libraries novel high affinity cyclic RGD peptides...

Impact of Cosmic Rays on Stratospheric Chlorine Chemistry and Ozone Depletion (2003)

Müller, Rolf

To view the published open abstract, go to http://dx.doi.org/10.1103/PhysRevLett.91.058502.

Weak impact of mixing on chlorine deactivation during SOLVE/THESEO 2000: Lagrangian modeling (CLaMS) versus ER-2 in situ observations (2003)

Konopka, Paul, Grooß, Jens-Uwe, Günther, Gebhard, McKenna, Daniel S., Müller, Rolf, Elkins, James W., ...

The original publication is available at http://dx.doi.org/10.1029/2001JD000876 . An edited version of this paper was published by AGU. Copyright 2003 American Geophysical Union.

Very early chlorine activation and ozone loss in the Arctic winter 2002–2003 (2003)

Tilmes, Simone, Müller, Rolf, Grooß, Jens-Uwe, Höpfner, Michael, Toon, Geoffrey C., Russell III, James M.

To view the published open abstract, go to http://dx.doi.org/10.1029/2003GL018079 . An edited version of this paper was published by AGU. Copyright 2003 American Geophysical Union.

Vertical profiles of activated ClO and ozone loss in the Arctic vortex in January and March 2000: In situ observations and model simulations (2003)

Vogel, Bärbel, Grooß, Jens-Uwe, Müller, Rolf, Deshler, Terry, Karhu, Juha, McKenna, Daniel S., ...

To view the published open abstract, go to http://dx.doi.org/10.1029/2002JD002564 . An edited version of this paper was published by AGU. Copyright 2003 American Geophysical Union.

A new Chemical Lagrangian Model of the Stratosphere (CLaMS) 2. Formulation of chemistry scheme and initialization (2002)

McKenna, Daniel S., Grooß, Jens-Uwe, Günther, Gebhard, Konopka, Paul, Müller, Rolf

To view the published open abstract, go to http://dx.doi.org/10.1029/2000JD000113 . An edited version of this paper was published by AGU. Copyright 2002 American Geophysical Union.

Chlorine activation and chemical ozone loss deduced from HALOE and balloon measurements in the Arctic during the winter of 1999–2000 (2002)

Müller, Rolf, Tilmes, Simone, Grooß, Jens-Uwe, McKenna, Daniel S., Müller, Melanie, Schmidt, Ulrich, ...

To view the published open abstract, go to http://dx.doi.org/10.1029/2001JD001423 . An edited version of this paper was published by AGU. Copyright 2002 American Geophysical Union.

A new Chemical Lagrangian Model of the Stratosphere (CLaMS) 1. Formulation of advection and mixing (2002)

McKenna, Daniel S., Konopka, Paul, Grooß, Jens-Uwe, Günther, Gebhard, Müller, Rolf, Spang, Reinhold, ...

To view the published open abstract, go to http://dx.doi.org/10.1029/2000JD000114 . An edited version of this paper was published by AGU. Copyright 2002 American Geophysical Union.

A new Chemical Lagrangian Model of the Stratosphere (CLaMS) 1. Formulation of advection and mixing (2002)

McKenna, Daniel S., Konopka, Paul, Grooß, Jens-Uwe, Günther, Gebhard, Müller, Rolf

To view the published open abstract, go to http://dx.doi.org/10.1029/2000JD000114 . An edited version of this paper was published by AGU. Copyright 2002 American Geophysical Union.

The mtaA Gene of the Myxothiazol Biosynthetic Gene Cluster from Stigmatella aurantiaca DW4/3-1 Encodes a Phosphopantetheinyl Transferase that Activates Polyketide Synthases and Polypeptide Synthetases (2001)

Gaitatzis, Nikolaos, Hans, Astrid, Müller, Rolf, Beyer, Stefan

Myxothiazol is synthesized by the myxobacterium Stigmatella aurantiaca DW4/3–1 via a combined polyketide synthase/polypeptide synthetase. The biosynthesis of this secondary metabolite is...

Optimized linker sequences for the expression of monomeric and dimeric bispecific single-chain diabodies (2001)

Völkel, Tina, Korn, Tina, Bach, Miriam, Müller, Rolf, Kontermann, Roland E.

Bispecific single-chain diabodies (scDb) consist of the variable heavy and light chain domains of two antibodies connected by three linkers. The structure of an scDb in the VH–VL orientation is...

Coarse Qualitative Descriptions in Robot Navigation (2000)

Rolf Müller, Thomas Röfer, Axel Lankenau, Alexandra Musto, Ra Musto, Klaus Stein, ...

This work is about the integration of the skills robot control, landmark recognition, and qualitative reasoning in a single autonomous mobile system. It deals with the transfer of coarse qualitative...

Coarse Qualitative Descriptions (2000)

In Robot Navigation, Rolf Müller, Thomas Röfer, Axel Lankenau, Ra Musto, Klaus Stein, ...

This work is about the integration of the skills robot control, landmark recognition, and qualitative reasoning in a single autonomous mobile system. It deals with the transfer of coarse qualitative...

A Taxonomy of Spatial Knowledge for Navigation and its Application to the Bremen Autonomous Wheelchair (1998)

Bernd Krieg-Brückner, Thomas Röfer, Hans-otto Carmesin, Rolf Müller

A new taxonomy is proposed that relates different navigational behaviors in a hierarchical and compositional way. Elementary navigation tactics are combined to tactical navigation in routes;...

Navigation and Routemark-Detection of the Bremen Autonomous Wheelchair (1998)

Thomas Röfer, Rolf Müller

This paper introduces the concept of a psychologically inspired navigation approach for an autonomous wheelchair. It presents the implementation of the architecture's basic modules and discusses...

Cell cycle regulation of cdc25C transcription is mediated by the periodic repression of the glutamine-rich activators NF-Y and Sp1 (1995)

Zwicker, Jörk, Gross, Claudia, Lucibello, Frances C., Truss, Mathias, Ehiert, Frauke, Engeland, Kurt, ...

The late S/G2-specific transcription of the human cdc25C gene is dependent on an initiator-proximal repressor element (CDE) and an upstream activating sequence (UAS) of undefined nature. We now show...

Non-leucine residues in the leucine repeats of Fos and Jun contribute to the stability and determine the specificity of dimerization (1991)

Schuermann, Marcus, Hunter, John B., Hennig, Guido, Müller, Rolf

Various transcription factors, including C/EBP, GCN4 and members of the Fos, Jun and Myc families have been shown to form highly specific complexes via αhelical structures referred to as leucine...

Multiple interdependent regulatory sites in the mouse c-fos promoter determine basal level transcription: cell type-specific effects (1991)

Lucibello, Frances C., Ehlert, Frauke, Müller, Rolf

Although the induction of the mouse c-fos promoter by growth factors and specific signal transduction pathways has been analyzed in some detail, the mechanisms involved in the control of basal level...

Similar rate of O6-ethylguanine elimination from DNA in normal human fibroblast and xeroderma pigmentosum cell strains not transformed by SV40 (1983)

Teo, Ian A., Lehmann, Alan R., Müller, Rolf, Rajewsky, Manfred F.

Using a highly specific rabbit antiserum (E3) directed against O6-ethyldeoxyguanosine in a competitive radioimmunoassay, we have measured the kinetics of removal of this alkylation product from the...

In vitro reconstitution of the myxochelin biosynthetic machinery of Stigmatella aurantiaca Sg a15: Biochemical characterization of a reductive release mechanism from nonribosomal peptide synthetases

Gaitatzis, Nikolaos, Kunze, Brigitte, Müller, Rolf

Microorganisms produce iron-chelating compounds to sequester the iron essential for growth from the environment. Many of these compounds are biosynthesized by nonribosomal peptide synthetases, some...

Inhibition of Cyclin-Dependent Kinase Activity and Induction of Apoptosis by Preussin in Human Tumor Cells

Achenbach, Tatjana V., Slater, Emily P., Brummerhop, Harm, Bach, Thorsten, Müller, Rolf

In this paper, we report that (+)-preussin, a pyrrolidinol alkaloid originally identified as an antifungal agent, has growth-inhibitory and cytotoxic effects on human cancer cells. Preussin was found...

CIF150, a Human Cofactor for Transcription Factor IID-Dependent Initiator Function

Kaufmann, Jörg, Ahrens, Katharina, Koop, Ronald, Smale, Stephen T., Müller, Rolf

The transcription factor IID (TFIID) complex is highly conserved between the Drosophila and mammalian systems. A mammalian homolog has been described for all the Drosophila TATA box-binding...

Searching for Interferon-Induced Genes That Inhibit Hepatitis B Virus Replication in Transgenic Mouse Hepatocytes†

Wieland, Stefan F., Vega, Raquel G., Müller, Rolf, Evans, Claire F., Hilbush, Brian, Guidotti, Luca G., ...

We have previously shown that alpha/beta interferon (IFN-α/β) and IFN-γ inhibit hepatitis B virus (HBV) replication noncytopathically in the livers of HBV transgenic mice and in hepatocyte cell...

Villidin, a Novel WD-repeat and Villin-related Protein from Dictyostelium, Is Associated with Membranes and the Cytoskeleton

Gloss, Annika, Rivero, Francisco, Khaire, Nandkumar, Müller, Rolf, Loomis, William F., Schleicher, Michael, ...

Villidin is a novel multidomain protein (190 kDa) from Dictyostelium amoebae containing WD repeats at its N-terminus, three PH domains in the middle of the molecule, and five gelsolin-like segments...

The Cyclase-associated Protein CAP as Regulator of Cell Polarity and cAMP Signaling in Dictyostelium

Noegel, Angelika A., Blau-Wasser, Rosemarie, Sultana, Hameeda, Müller, Rolf, Israel, Lars, Schleicher, Michael, ...

Cyclase-associated protein (CAP) is an evolutionarily conserved regulator of the G-actin/F-actin ratio and, in yeast, is involved in regulating the adenylyl cyclase activity. We show that cell...

Biosynthetic Pathway of Pseudomonas aeruginosa 4-Hydroxy-2-Alkylquinolines

Bredenbruch, Florian, Nimtz, Manfred, Wray, Victor, Morr, Michael, Müller, Rolf, Häussler, Susanne

The role of intercellular communication in the regulation of bacterial multicellular behavior has received widespread attention, and a variety of signal molecules involved in bacterial communication...

The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles

Wilkins, Andrew, Szafranski, Karol, Fraser, Derek J, Bakthavatsalam, Deenadayalan, Müller, Rolf, Fisher, Paul R, ...

A survey of the Dictyostelium genome reveals at least 25 RasGEFs, all of which appear to be expressed at some point in development. Disruption of several of these novel RasGEFs reveals that many have...

Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis

Berner, Martin, Krug, Daniel, Bihlmaier, Corina, Vente, Andreas, Müller, Rolf, Bechthold, Andreas

The saccharomicins A and B, produced by the actinomycete Saccharothrix espanaensis, are oligosaccharide antibiotics. They consist of 17 monosaccharide units and the unique aglycon...

In vitro reconstitution of the myxochelin biosynthetic machinery of Stigmatella aurantiaca Sg a15: Biochemical characterization of a reductive release mechanism from nonribosomal peptide synthetases

Gaitatzis, Nikolaos, Kunze, Brigitte, Müller, Rolf

Microorganisms produce iron-chelating compounds to sequester the iron essential for growth from the environment. Many of these compounds are biosynthesized by nonribosomal peptide synthetases, some...

Inhibition of Cyclin-Dependent Kinase Activity and Induction of Apoptosis by Preussin in Human Tumor Cells

Achenbach, Tatjana V., Slater, Emily P., Brummerhop, Harm, Bach, Thorsten, Müller, Rolf

In this paper, we report that (+)-preussin, a pyrrolidinol alkaloid originally identified as an antifungal agent, has growth-inhibitory and cytotoxic effects on human cancer cells. Preussin was found...

CIF150, a Human Cofactor for Transcription Factor IID-Dependent Initiator Function

Kaufmann, Jörg, Ahrens, Katharina, Koop, Ronald, Smale, Stephen T., Müller, Rolf

The transcription factor IID (TFIID) complex is highly conserved between the Drosophila and mammalian systems. A mammalian homolog has been described for all the Drosophila TATA box-binding...

Searching for Interferon-Induced Genes That Inhibit Hepatitis B Virus Replication in Transgenic Mouse Hepatocytes†

Wieland, Stefan F., Vega, Raquel G., Müller, Rolf, Evans, Claire F., Hilbush, Brian, Guidotti, Luca G., ...

We have previously shown that alpha/beta interferon (IFN-α/β) and IFN-γ inhibit hepatitis B virus (HBV) replication noncytopathically in the livers of HBV transgenic mice and in hepatocyte cell...

Villidin, a Novel WD-repeat and Villin-related Protein from Dictyostelium, Is Associated with Membranes and the Cytoskeleton

Gloss, Annika, Rivero, Francisco, Khaire, Nandkumar, Müller, Rolf, Loomis, William F., Schleicher, Michael, ...

Villidin is a novel multidomain protein (190 kDa) from Dictyostelium amoebae containing WD repeats at its N-terminus, three PH domains in the middle of the molecule, and five gelsolin-like segments...

The Cyclase-associated Protein CAP as Regulator of Cell Polarity and cAMP Signaling in Dictyostelium

Noegel, Angelika A., Blau-Wasser, Rosemarie, Sultana, Hameeda, Müller, Rolf, Israel, Lars, Schleicher, Michael, ...

Cyclase-associated protein (CAP) is an evolutionarily conserved regulator of the G-actin/F-actin ratio and, in yeast, is involved in regulating the adenylyl cyclase activity. We show that cell...

Biosynthetic Pathway of Pseudomonas aeruginosa 4-Hydroxy-2-Alkylquinolines

Bredenbruch, Florian, Nimtz, Manfred, Wray, Victor, Morr, Michael, Müller, Rolf, Häussler, Susanne

The role of intercellular communication in the regulation of bacterial multicellular behavior has received widespread attention, and a variety of signal molecules involved in bacterial communication...

The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles

Wilkins, Andrew, Szafranski, Karol, Fraser, Derek J, Bakthavatsalam, Deenadayalan, Müller, Rolf, Fisher, Paul R, ...

A survey of the Dictyostelium genome reveals at least 25 RasGEFs, all of which appear to be expressed at some point in development. Disruption of several of these novel RasGEFs reveals that many have...

Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis

Berner, Martin, Krug, Daniel, Bihlmaier, Corina, Vente, Andreas, Müller, Rolf, Bechthold, Andreas

The saccharomicins A and B, produced by the actinomycete Saccharothrix espanaensis, are oligosaccharide antibiotics. They consist of 17 monosaccharide units and the unique aglycon...

3-Hydroxy-3-Methylglutaryl-Coenzyme A (CoA) Synthase Is Involved in Biosynthesis of Isovaleryl-CoA in the Myxobacterium Myxococcus xanthus during Fruiting Body Formation

Bode, Helge B., Ring, Michael W., Schwär, Gertrud, Kroppenstedt, Reiner M., Kaiser, Dale, Müller, Rolf

Isovaleryl-coenzyme A (IV-CoA) is the starting unit for some secondary metabolites and iso-odd fatty acids in several bacteria. According to textbook biochemistry, IV-CoA is derived from leucine...

Reconstitution of the Myxothiazol Biosynthetic Gene Cluster by Red/ET Recombination and Heterologous Expression in Myxococcus xanthus▿

Perlova, Olena, Fu, Jun, Kuhlmann, Silvia, Krug, Daniel, Stewart, A. Francis, Zhang, Youming, ...

Although many secondary metabolites exhibiting important pharmaceutical and agrochemical activities have been isolated from myxobacteria, most of these microorganisms remain difficult to handle...

The unique DKxanthene secondary metabolite family from the myxobacterium Myxococcus xanthus is required for developmental sporulation

Meiser, Peter, Bode, Helge B., Müller, Rolf

Under starvation conditions myxobacteria form multicellular fruiting bodies in which vegetative cells differentiate into heat- and desiccation-resistant myxospores. Myxobacteria in general are a rich...

GeneTrail—advanced gene set enrichment analysis

Backes, Christina, Keller, Andreas, Kuentzer, Jan, Kneissl, Benny, Comtesse, Nicole, Elnakady, Yasser A., ...

We present a comprehensive and efficient gene set analysis tool, called ‘GeneTrail’ that offers a rich functionality and is easy to use. Our web-based application facilitates the statistical...

A Role for PPARβ/δ in Tumor Stroma and Tumorigenesis

Müller, Rolf, Kömhoff, Martin, Peters, Jeffrey M., Müller-Brüsselbach, Sabine

Peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) is a transcription factor that is activated by endogenous fatty acid ligands and by synthetic agonists. Its role in the regulation of...

Deregulation of tumor angiogenesis and blockade of tumor growth in PPARβ-deficient mice

Müller-Brüsselbach, Sabine, Kömhoff, Martin, Rieck, Markus, Meissner, Wolfgang, Kaddatz, Kerstin, Adamkiewicz, Jürgen, ...

The peroxisome proliferator-activated receptor-β (PPARβ) has been implicated in tumorigenesis, but its precise role remains unclear. Here, we show that the growth of syngeneic Pparb wild-type...

Discovering the Hidden Secondary Metabolome of Myxococcus xanthus: a Study of Intraspecific Diversity▿

Krug, Daniel, Zurek, Gabriela, Revermann, Ole, Vos, Michiel, Velicer, Gregory J., Müller, Rolf

As a monophyletic group, the myxobacteria are known to produce a broad spectrum of secondary metabolites. However, the degree of metabolic diversity that can be found within a single species remains...

Regulation of Cell Proliferation and Differentiation by PPARβ/δ

Müller, Rolf, Rieck, Markus, Müller-Brüsselbach, Sabine

Peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) is a ligand-activated transcription factor with essential functions in the regulation of lipid catabolism, glucose homeostasis, and...

Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition

Fu, Jun, Wenzel, Silke C., Perlova, Olena, Wang, Junping, Gross, Frank, Tang, Zhiru, ...

Horizontal gene transfer by transposition has been widely used for transgenesis in prokaryotes. However, conjugation has been preferred for transfer of large transgenes, despite greater restrictions...

Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermopilic isolate Corallococcus macrosporus GT-2

Perlova, Olena, Gerth, Klaus, Kuhlmann, Silvia, Zhang, Youming, Müller, Rolf

Although many secondary metabolites with diverse biological activities have been isolated from myxobacteria, most strains of these biotechnologically important gliding prokaryotes remain difficult to...