Rolf Sprengel

Roles of the AMPA receptor subunit GluA1 but not GluA2 in synaptic potentiation and activation of ERK in the anterior cingulate cortex (2009)

Toyoda, Hiroki, Zhao, Ming-Gao, Ulzhöfer, Bettina, Wu, Long-Jun, Xu, Hui, Seeburg, Peter H, ...

Abstract Cortical areas including the anterior cingulate cortex (ACC) are important for pain and pleasure. Recent studies using genetic and physiological approaches have demonstrated that the...

Enhanced long-term and impaired short-term spatial memory in GluA1 AMPA receptor subunit knockout mice: Evidence for a dual-process memory model (2009)

Sanderson, David J., Good, Mark A., Skelton, Kathryn, Sprengel, Rolf, Seeburg, Peter H., Rawlins, J. Nicholas P., ...

The GluA1 AMPA receptor subunit is a key mediator of hippocampal synaptic plasticity and is especially important for a rapidly-induced, short-lasting form of potentiation. GluA1 gene deletion impairs...

Behavioral/Systems/Cognitive The Role of Hippocampal Glutamate Receptor-A-Dependent Synaptic Plasticity in Conditional Learning: The Importance (2008)

Of Spatiotemporal Discontiguity, Wolfram B. Schmitt, Roozbeh Arianpour, Peter H. Seeburg, Rolf Sprengel, ...

Gene-targeted mice lacking the AMPA receptor subunit glutamate receptor-A (GluR-A or GluR1) and mice with cytotoxic hippocampal lesions were compared with wild-type and sham-operated controls,...

Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor (2008)

Haydon−Wallace, Damian J., Astori, Simone, Yang, Ying, Bausen, Melanie, Kügler, Sebastian, ...

Measurement of population activity with single−action−potential, single−neuron resolution is pivotal for understanding information representation and processing in the brain and how the...

Suitability of tamoxifen−induced mutagenesis for behavioral phenotyping (2008)

Vogt, M. A., Chourbaji, S., Brandwein, C., Dormann, C., Sprengel, Rolf, Gass, P.

Tamoxifen−induced mutagenesis via the so−called CreERT2 fusion enzyme is a key technology for the inducible gene knockout in the adult murine brain. However, it requires a subchronic transient...

Conditional transgenic mouse models: from the basics to genome−wide sets of knockouts and current studies of tissue regeneration (2008)

Bockamp, Ernesto, Sprengel, Rolf, Eshkind, Leonid, Lehmann, Thomas, Braun, Jan M., Emmrich, Frank, ...

Many mouse models are currently available, providing avenues to elucidate gene function and to recapitulate specific pathological conditions. To a large extent, successful translation of clinical...

Glutamate mediates platelet activation through the AMPA receptor (2008)

Morrell, C. N., Sun, H., Ikeda, M., Beique, J. C., Swaim, A. M., Mason, E., ...

Glutamate is an excitatory neurotransmitter that binds to the kainate receptor, the N−methyl−D−aspartate (NMDA) receptor, and the...

NMDA receptor subunit NR2A is required for rapidly acquired spatial working memory but not incremental spatial reference memory (2008)

Bannerman, David M., Niewoehner, Burkhard, Lyon, Louisa, Romberg, Carola, Schmitt, Wolfram B., Taylor, Amy, ...

NMDA receptors (NMDARs) containing NR2A ({varepsilon}1) subunits are key contributors to hippocampal long−term potentiation (LTP) induction in adult animals and have therefore been widely...

The role of the GluR−A (GluR1) AMPA receptor subunit in learning and memory (2008)

Sanderson, D. J., Good, M. A., Seeburg, Peter H., Sprengel, Rolf, Rawlins, J. N. P., Bannerman, D. M.

It is widely believed that synaptic plasticity may provide the neural mechanism that underlies certain kinds of learning and memory in the mammalian brain. The expression of long−term potentiation...

AMPA receptor subunit 1 (GluR−A) knockout mice model the glutamate hypothesis of depression (2008)

Chourbaji, S., Vogt, M. A., Fumagalli, F., Sohr, R., Frasca, A., Brandwein, C., ...

Recent evidence indicates that glutamate homeostasis and neurotransmission are altered in major depressive disorder, but the nature of the disruption and the mechanisms by which it contributes to the...

Developmental impact of a familial GABAA receptor epilepsy mutation (2008)

Chiu, Cindy, Reid, Christopher A., Heneu, O. Tan, Davies, Philip J., Single, Frank Nicolai, Koukoulas, Irene, ...

Epilepsy, with a lifetime prevalence rate of 3%, is a common and serious neurological disorder. It is characterized by recurrent paroxysms resulting from hypersynchronous discharges in the brain....

Glutamate receptors on dopamine neurons control the persistence of cocaine seeking (2008)

Engblom, David, Bilbao, Ainhoa, Sanchis−Segura, Carles, Dahan, Lionel, Perreau−Lenz, Stéphanie, Balland, Bénédicte, ...

Cocaine strengthens excitatory synapses onto midbrain dopamine neurons through the synaptic delivery of GluR1−containing AMPA receptors. This cocaine−evoked plasticity depends on NMDA receptor...

Activity−dependent potentiation of calcium signals in spinal sensory networks in inflammatory pain states (2008)

Luo, Ceng, Seeburg, Peter H., Sprengel, Rolf, Kuner, Rohini

The second messenger calcium is a key mediator of activity−dependent neural plasticity. How persistent nociceptive activity alters calcium influx and release in the spinal cord is not...

Role of GluR1 in activity−dependent motor system development (2008)

Zhang, Lujia, Schessl, Joachim, Werner, Markus, Bonnemann, Carsten, Xiong, Guoxiang, Mojsilovic−Petrovic, Jelena, ...

Activity−dependent specification of neuronal architecture during early postnatal life is essential for refining the precision of communication between neurons. In the spinal cord under normal...

Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor (2008)

Haydon−Wallace, Damian J., Astori, Simone, Yang, Ying, Bausen, Melanie, Kügler, Sebastian, ...

Measurement of population activity with single−action−potential, single−neuron resolution is pivotal for understanding information representation and processing in the brain and how the...

Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor (2008)

Haydon−Wallace, Damian J., Astori, Simone, Yang, Ying, Bausen, Melanie, Kügler, Sebastian, ...

Measurement of population activity with single−action−potential, single−neuron resolution is pivotal for understanding information representation and processing in the brain and how the...

Mice lacking the AMPA GluR1 receptor exhibit striatal hypodopaminergia and 'schizophrenia−related' behaviors (2007)

Wiedholz, L. M., Owens, W. A., Horton, R. E., Feyder, M., Karlsson, R.−M., Hefner, K., ...

There is growing evidence implicating dysfunctional glutamatergic neurotransmission and abnormal interactions between the glutamate and dopamine (DA) systems in the pathophysiology of various...

Reduced cortical inhibition in a mouse model of familial childhood absence epilepsy (2007)

Tan, Heneu, Reid, Christopher, Single, Frank Nicolai, Davies, Philip, Chiu, Cindy, Murphy, Susan, ...

Mutations in the GABAA receptor {gamma}2 subunit are associated with childhood absence epilepsy and febrile seizures. To understand better the molecular basis of absence epilepsy in man, we developed...

Mice lacking the AMPA GluR1 receptor exhibit striatal hypodopaminergia and 'schizophrenia−related' behaviors (2007)

Wiedholz, L. M., Owens, W. A., Horton, R. E., Feyder, M., Karlsson, R.−M., Hefner, K., ...

There is growing evidence implicating dysfunctional glutamatergic neurotransmission and abnormal interactions between the glutamate and dopamine (DA) systems in the pathophysiology of various...

Remembering the subtle differences (2007)

Bannerman, David M., Sprengel, Rolf

A good taxi driver in a cosmopolitan city has to find an arbitrary destination from any starting point, efficiently and with high precision, relying on recollection of the city's layout and...

Reduced cortical inhibition in a mouse model of familial childhood absence epilepsy (2007)

Tan, Heneu, Reid, Christopher, Single, Frank Nicolai, Davies, Philip, Chiu, Cindy, Murphy, Susan, ...

Mutations in the GABAA receptor {gamma}2 subunit are associated with childhood absence epilepsy and febrile seizures. To understand better the molecular basis of absence epilepsy in man, we developed...

A pathway−specific function for different AMPA receptor subunits in amygdala long−term potentiation and fear conditioning (2007)

Humeau, Yann, Reisel, Daniel, Johnson, Alexander W., Borchardt, Thilo, Jensen, Vidar, Gebhardt, Christine, ...

The AMPA receptor subunit glutamate receptor 1 (GluR1 or GluR−A) contributes to amygdala−dependent emotional learning. It remains unclear, however, to what extent different amygdala pathways...

Impaired spatial working memory but spared spatial reference memory following functional loss of NMDA receptors in the dentate gyrus (2007)

Niewoehner, B., Single, Frank Nicolai, Hvalby, Ø., Jensen, V., Seeburg, Peter H., ...

Novel spatially restricted genetic manipulations can be used to assess contributions made by synaptic plasticity to learning and memory, not just selectively within the hippocampus, but even within...

Forebrain−specific glutamate receptor B deletion impairs spatial memory but not hippocampal field long−term potentiation (2006)

Shimshek, Derya R., Jensen, Vidar, Celikel, Tansu, Geng, Yu, Schupp, Bettina, Bus, Thorsten, ...

We demonstrate the fundamental importance of glutamate receptor B (GluR−B) containing AMPA receptors in hippocampal function by analyzing mice with conditional GluR−B deficiency in postnatal...

Forebrain−specific glutamate receptor B deletion impairs spatial memory but not hippocampal field long−term potentiation (2006)

Shimshek, Derya R., Jensen, Vidar, Celikel, Tansu, Geng, Yu, Schupp, Bettina, Bus, Thorsten, ...

We demonstrate the fundamental importance of glutamate receptor B (GluR−B) containing AMPA receptors in hippocampal function by analyzing mice with conditional GluR−B deficiency in postnatal...

Role of AMPA receptors in synaptic plasticity (2006)

Sprengel, Rolf

The a (alpha−)−amino−3−hydroxy−5−methyl−4−isoxazolepropionic acid (AMPA) receptors are the principal molecular units for fast excitatory synaptic transmission in the central nervous...

MEGAP impedes cell migration via regulating actin and microtubule dynamics and focal complex formation (2006)

Yang, Ying, Marcello, Marco, Endris, Volker, Saffrich, Rainer, Fischer, Roger, Trendelenburg, Michael, ...

Over the past several years, it has become clear that the Rho family of GTPases plays an important role in various aspects of neuronal development including cytoskeleton dynamics and cell adhesion...

Tetracycline−controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c−kit−expressing lineage−negative hematopoietic cells (2006)

Bockamp, Ernesto, Antunes, Cecilia, Maringer, Marko, Heck, Rosario, Presser, Katrin, Beilke, Sven, ...

The stem cell leukemia gene SCL, also known as TAL−1, encodes a basic helix−loop−helix transcription factor expressed in erythroid, myeloid, megakaryocytic, and hematopoietic stem cells. To be...

Involvement of the AMPA receptor GluR−C subunit in alcohol−seeking behavior and relapse (2006)

Sanchis−Segura, Carles, Borchardt, Thilo, Vengeliene, Valentina, Zghoul, Tarek, Bachteler, Daniel, Gass, Peter, ...

Craving and relapse are core symptoms of drug addiction and alcoholism. It is suggested that, after chronic drug consumption, long−lasting neuroplastic changes within the glutamatergic system are...

NMDA receptors in layer 4 spiny stellate cells of the mouse barrel cortex contain the NR2C subunit (2006)

Binshtok, Alexander, Fleidervish, Ilya, Sprengel, Rolf, Gutnick, Michael

In layer 4 of the somatosensory cortex, the glutamatergic synapses that interconnect spiny stellate (SpS) neurons, which are the major targets of thalamocortical input, differ from most other...

Enhanced Odor Discrimination and Impaired Olfactory Memory by Spatially Controlled Switch of AMPA Receptors (2005)

Derya R. Shimshek, Thorsten Bus, Jinhyun Kim, Andre Mihaljevic, Volker Mack, Peter H. Seeburg, ...

Genetic manipulation of AMPA receptors in mouse forebrain dissects specific contributions of AMPA receptor expression variation in piriform cortex and hippocampus to olfactory learning and memory,...

Enhanced Odor Discrimination and Impaired Olfactory Memory by Spatially Controlled Switch of AMPA Receptors (2005)

Derya R. Shimshek, Thorsten Bus, Jinhyun Kim, Andre Mihaljevic, Volker Mack, Peter H. Seeburg, ...

Genetic perturbations of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) are widely used to dissect molecular mechanisms of sensory coding, learning, and memory. In this study,...

Enhanced odor discrimination and impaired olfactory memory by spatially controlled switch of AMPA Receptors (2005)

Shimshek, Derya R., Bus, Thorsten, Kim, Jinhyun, Mihaljevic, André, Mack, Volker, Seeburg, Peter H., ...

Genetic perturbations of á−amino−3−hydroxy−5−methyl−4−isoxazolepropionate receptors (AMPARs) are widely used to dissect molecular mechanisms of sensory coding, learning, and memory. In...

Restoration of spatial working memory by genetic rescue of GluR−A−deficient mice (2005)

Schmitt, W., Sprengel, Rolf, Mack, Volker, Draft, Ryan, Seeburg, Peter H., Deacon, Robert M. J., ...

Gene−targeted mice lacking the AMPA receptor subunit GluR−A (also called GluR1 encoded by the gene Gria1,) have deficits in hippocampal CA3−CA1 long−term potentiation (LTP) and have...

Enhanced odor discrimination and impaired olfactory memory by spatially controlled switch of AMPA Receptors (2005)

Shimshek, Derya R., Bus, Thorsten, Kim, Jinhyun, Mihaljevic, André, Mack, Volker, Seeburg, Peter H., ...

Genetic perturbations of á−amino−3−hydroxy−5−methyl−4−isoxazolepropionate receptors (AMPARs) are widely used to dissect molecular mechanisms of sensory coding, learning, and memory. In...

A genetic switch for epilepsy in adult mice (2004)

Krestel,Heinz Eric, Shimshek,Derya R., Jensen,Vidar, Nevian,Thomas, Kim,Jin−Hyun, Geng,Yu, ...

Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...

A genetic switch for epilepsy in adult mice (2004)

Krestel,Heinz Eric, Shimshek,Derya R., Jensen,Vidar, Nevian,Thomas, Kim,Jin−Hyun, Geng,Yu, ...

Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...

The AMPA receptor subunits GluR−A and GluR−B reciprocally modulate spinal synaptic plasticity and inflammatory pain (2004)

Hartmann,Bettina, Ahmadi,Seifollah, Heppenstall,Paul, Lewin,Gary, Schott,Claus, Borchardt,Thilo, ...

Ca2+−permeable AMPA receptors are densely expressed in the spinal dorsal horn, but their functional significance in pain processing is not understood. By disrupting the genes encoding GluR−A or...

Mice with a fra−1 knock−in into the c−fos locus show impaired spatial but regular contextual learning and normal LTP (2004)

Gass,Peter, Fleischmann,Alexander, Hvalby,Øivind, Jensen,Vidar, Zacher,Christiane, Strekalova,Tatyana, ...

The immediate early gene c−fos is part of the AP−1 transcription factor complex, which is involved in molecular mechanisms underlying learning and memory. Mice that lack c−Fos in the brain show...

Reduced aggression in AMPA−type glutamate receptor GluR−A subunit−deficient mice (2004)

Vekovischeva,Olga Y., Aitta−Aho,T., Echenko,O., Kankaanpaa,A., Seppala,T., Honkanen,A., ...

The importance of AMPA−type glutamate receptors has been demonstrated in neuronal plasticity and in adaptation to drugs of abuse. We studied the involvement of AMPA receptors in social interaction...

Neuronal Toxicity in Caenorhabditis elegans from an Editing Site Mutant in Glutamate Receptor Channels (2004)

Aronoff,Rachel, Mellem,Jerry E., Maricq,Andres Villu, Sprengel,Rolf, Seeburg,Peter H.

Ionotropic glutamate receptors (iGluRs) in Caenorhabditis elegans are predicted to have high permeability for Ca2+ because of glutamine (Q) residues in the pore loop. This contrasts to the low Ca2+...

The role of hippocampal glutamate receptor−A−dependent synaptic plasticity in conditional learning: the importance of spatio−temporal discontiguity (2004)

Schmitt,Wolfram B., Arianpour,Roozbeh, Deacon,Robert M. J., Seeburg,Peter H., Sprengel,Rolf, Rawlins,J. Nicholas P., ...

Gene−targeted mice lacking the AMPA receptor subunit glutamate receptor−A (GluR−A or GluR1) and mice with cytotoxic hippocampal lesions were compared with wild−type and sham−operated...

A comparison of GluR−A−deficient and wild−type mice on a test battery assessing sensorimotor, affective, and cognitive behaviors (2004)

Bannerman,David M., Deacon,Robert M. J., Brady,S., Bruce,A., Sprengel,Rolf, Seeburg,Peter H., ...

Previous studies have demonstrated a spatial working memory deficit in glutamate receptor (GluR)−A (GluR1) AMPA receptor subunit knockout mice. The present study evaluated male and female...

Spatial reference memory in GluR−A−deficient mice using a novel hippocampal−dependent paddling pool escape task (2004)

Schmitt,Wolfram B., Deacon,Robert M. J., Reisel,D., Sprengel,Rolf, Seeburg,Peter H., Rawlins,J. Nicholas P., ...

Genetically modified mice lacking the L−−amino−3−hydroxy−5−methylisoxazole−4−propionate (AMPA) receptor subunit, GluR−A (GluR1), and deficient in hippocampal CA3−CA1 long−term...

Spatial reference memory in GluR−A−deficient mice using a novel hippocampal−dependent paddling pool escape task (2004)

Schmitt,Wolfram B., Deacon,Robert M. J., Reisel,D., Sprengel,Rolf, Seeburg,Peter H., Rawlins,J. Nicholas P., ...

Genetically modified mice lacking the L−−amino−3−hydroxy−5−methylisoxazole−4−propionate (AMPA) receptor subunit, GluR−A (GluR1), and deficient in hippocampal CA3−CA1 long−term...

Spatial reference memory in GluR−A−deficient mice using a novel hippocampal−dependent paddling pool escape task (2004)

Schmitt, Wolfram B., Deacon, Robert M. J., Reisel, D., Sprengel, Rolf, Seeburg, Peter H., Rawlins, J. Nicholas P., ...

Genetically modified mice lacking the L−−amino−3−hydroxy−5−methylisoxazole−4−propionate (AMPA) receptor subunit, GluR−A (GluR1), and deficient in hippocampal CA3−CA1 long−term...

A genetic switch for epilepsy in adult mice (2004)

Krestel, Heinz Eric, Shimshek, Derya R., Jensen, Vidar, Nevian, Thomas, Kim, Jin−Hyun, Geng, Yu, ...

Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...

A genetic switch for epilepsy in adult mice (2004)

Krestel, Heinz Eric, Shimshek, Derya R., Jensen, Vidar, Nevian, Thomas, Kim, Jin−Hyun, Geng, Yu, ...

Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...

A comparison of GluR−A−deficient and wild−type mice on a test battery assessing sensorimotor, affective, and cognitive behaviors (2004)

Bannerman, David M., Deacon, Robert M. J., Brady, S., Bruce, A., Sprengel, Rolf, Seeburg, Peter H., ...

Previous studies have demonstrated a spatial working memory deficit in glutamate receptor (GluR)−A (GluR1) AMPA receptor subunit knockout mice. The present study evaluated male and female...

Mice with a fra−1 knock−in into the c−fos locus show impaired spatial but regular contextual learning and normal LTP (2004)

Gass, Peter, Fleischmann, Alexander, Hvalby, Øivind, Jensen, Vidar, Zacher, Christiane, Strekalova, Tatyana, ...

The immediate early gene c−fos is part of the AP−1 transcription factor complex, which is involved in molecular mechanisms underlying learning and memory. Mice that lack c−Fos in the brain show...

Reduced aggression in AMPA−type glutamate receptor GluR−A subunit−deficient mice (2004)

Vekovischeva, Olga Y., Aitta−Aho, T., Echenko, O., Kankaanpaa, A., Seppala, T., Honkanen, A., ...

The importance of AMPA−type glutamate receptors has been demonstrated in neuronal plasticity and in adaptation to drugs of abuse. We studied the involvement of AMPA receptors in social interaction...

The AMPA receptor subunits GluR−A and GluR−B reciprocally modulate spinal synaptic plasticity and inflammatory pain (2004)

Hartmann, Bettina, Ahmadi, Seifollah, Heppenstall, Paul, Lewin, Gary, Schott, Claus, Borchardt, Thilo, ...

Ca2+−permeable AMPA receptors are densely expressed in the spinal dorsal horn, but their functional significance in pain processing is not understood. By disrupting the genes encoding GluR−A or...

Neuronal Toxicity in Caenorhabditis elegans from an Editing Site Mutant in Glutamate Receptor Channels (2004)

Aronoff, Rachel, Mellem, Jerry E., Maricq, Andres Villu, Sprengel, Rolf, Seeburg, Peter H.

Ionotropic glutamate receptors (iGluRs) in Caenorhabditis elegans are predicted to have high permeability for Ca2+ because of glutamine (Q) residues in the pore loop. This contrasts to the low Ca2+...

The role of hippocampal glutamate receptor−A−dependent synaptic plasticity in conditional learning: the importance of spatio−temporal discontiguity (2004)

Schmitt, Wolfram B., Arianpour, Roozbeh, Deacon, Robert M. J., Seeburg, Peter H., Sprengel, Rolf, Rawlins, J. Nicholas P., ...

Gene−targeted mice lacking the AMPA receptor subunit glutamate receptor−A (GluR−A or GluR1) and mice with cytotoxic hippocampal lesions were compared with wild−type and sham−operated...

Spatial reference memory in GluR−A−deficient mice using a novel hippocampal−dependent paddling pool escape task (2004)

Schmitt, Wolfram B., Deacon, Robert M. J., Reisel, D., Sprengel, Rolf, Seeburg, Peter H., Rawlins, J. Nicholas P., ...

Genetically modified mice lacking the L−−amino−3−hydroxy−5−methylisoxazole−4−propionate (AMPA) receptor subunit, GluR−A (GluR1), and deficient in hippocampal CA3−CA1 long−term...

A juvenile form of postsynaptic hippocampal long-term potentiation in mice deficient for the AMPA receptor subunit GluR-A (2003)

Jensen,Vidar, Kaiser,Katharina M. M., Borchardt,Thilo, Adelmann,Giselind, Rozov,Andrei, Burnashev,Nail, ...

In adult mice, long-term potentiation (LTP) of synaptic transmission at CA3-to-CA1 synapses induced by tetanic stimulation requires L-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)...

Intracellular domains of the NMDA receptor subtypes are determinants for long-term potentiation induction (2003)

Köhr,Georg, Jensen,Vidar, Koester,Helmut J., Mihaljevic,Andre L. A., Utvik,Jo Kristian, Kvello,Ane, ...

NMDA receptors (NMDARs) are essential for modulating synaptic strength at central synapses. At hippocampal CA3-to-CA1 synapses of adult mice, different NMDAR subtypes with distinct functionality...

Impaired Long-Term Memory and NR2A-Type NMDA Receptor-Dependent Synaptic Plasticity in Mice Lacking c-Fos in the CNS (2003)

Fleischmann,Alexander, Hvalby,Øyvind, Jensen,Vidar, Strekalova,Tatyana, Zacher,Christiane, Layer,Liliana, ...

Long-term learning and memory require long-lasting changes in the strength of specific synaptic connections between neurons (Martin et al., 2000; Kandel, 2001). These adaptive changes in synaptic...

Impaired regulation of synaptic strength in hippocampal neurons from GluR1-deficient mice (2003)

Andrásfalvy,Bertalan K., Smith,Mark A., Borchardt,Thilo, Sprengel,Rolf, Magee,Jeffrey C.

Neurons of the central nervous system (CNS) exhibit a variety of forms of synaptic plasticity, including associative long-term potentiation and depression (LTP/D), homeostatic activity-dependent...

Impaired long-term memory and NlR2A-type NMDA receptor dependent synaptic plasticity in mice lacking c-Fos in the brain (2003)

Sprengel, Rolf, Spanagel, Rainer, Kvello, Ane, Layer, Liliana, Zacher, Christiane, Strekalova, Tatyana, ...

The immediate early gene c-fos is part of the activator protein-1 transcription factor and has been postulated to participate in the molecular mechanisms of learning and memory. To test this...

Impaired regulation of synaptic strength in hippocampal neurons from GluR1-deficient mice (2003)

Andrásfalvy, Bertalan K., Smith, Mark A., Borchardt, Thilo, Sprengel, Rolf, Magee, Jeffrey C.

Neurons of the central nervous system (CNS) exhibit a variety of forms of synaptic plasticity, including associative long-term potentiation and depression (LTP/D), homeostatic activity-dependent...

Impaired Long-Term Memory and NR2A-Type NMDA Receptor-Dependent Synaptic Plasticity in Mice Lacking c-Fos in the CNS (2003)

Fleischmann, Alexander, Hvalby, Øyvind, Jensen, Vidar, Strekalova, Tatyana, Zacher, Christiane, Layer, Liliana, ...

Long-term learning and memory require long-lasting changes in the strength of specific synaptic connections between neurons (Martin et al., 2000; Kandel, 2001). These adaptive changes in synaptic...

A juvenile form of postsynaptic hippocampal long-term potentiation in mice deficient for the AMPA receptor subunit GluR-A (2003)

Jensen, Vidar, Kaiser, Katharina M. M., Borchardt, Thilo, Adelmann, Giselind, Rozov, Andrei, Burnashev, Nail, ...

In adult mice, long-term potentiation (LTP) of synaptic transmission at CA3-to-CA1 synapses induced by tetanic stimulation requires L-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)...

Intracellular domains of the NMDA receptor subtypes are determinants for long-term potentiation induction (2003)

Köhr, Georg, Jensen, Vidar, Koester, Helmut J., Mihaljevic, Andre L. A., Utvik, Jo Kristian, Kvello, Ane, ...

NMDA receptors (NMDARs) are essential for modulating synaptic strength at central synapses. At hippocampal CA3-to-CA1 synapses of adult mice, different NMDAR subtypes with distinct functionality...

ER-based double iCRE fusion protein allows partial recombination in forebrain (2002)

Casanova,James E., Fehsenfeld,Sandra, Lemberger,Thomas, Shimshek,Derya R., Sprengel,Rolf, Mantamadiotis,Theo

Here we describe the generation of a new tamoxifen-inducible double Cre fusion protein generated by fusing two ERT2 domains onto both ends of the iCre recombinase (a codon improved Cre recombinase)....

A predictable ligand regulated expression strategy for stably integrated transgenes in mammalian cells in culture (2002)

Anastassiadis,Konstantinos, Kim,Jin-Hyun, Daigle,Nathalie, Sprengel,Rolf, Schöler,Hans R., Stewart,A. Francis

Several strategies for regulated stable transgene expression in mammalian cells have been described. These strategies have different strengths and weaknesses, however they all share a common problem,...

Molecular dissection of hippocampal theta-burst pairing potentiation (2002)

Hoffman,Dax A., Sprengel,Rolf, Sakmann,Bert

Long-term potentiation (LTP) of synaptic efficacy in the hippocampus is frequently induced by tetanic stimulation of presynaptic afferents or by pairing low frequency stimulation with postsynaptic...

Codon-improved Cre recombinase (iCre) expression in the mouse (2002)

Shimshek,Derya R., Kim,Jin-Hyun, Hübner,Michael R., Spergel,Daniel J., Buchholz,Frank, Casanova,James E., ...

By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre...

Deletion of the C-terminal domain of the NR2B subunit alters channel properties and synaptic targeting of NMDA receptors in nascent neocortical synapses (2002)

Mohrmann,Ralf, Köhr,Georg, Sprengel,Rolf, Hatt,Hanns, Gottmann,Kurt

Channel properties and synaptic targeting of N-methyl-D-aspartate (NMDA) receptors determine their importance in synaptic transmission, long-term synaptic plasticity, and developmental reorganization...

A predictable ligand regulated expression strategy for stably integrated transgenes in mammalian cells in culture (2002)

Anastassiadis, Konstantinos, Kim, Jin-Hyun, Daigle, Nathalie, Sprengel, Rolf, Schöler, Hans R., Stewart, A. Francis

Several strategies for regulated stable transgene expression in mammalian cells have been described. These strategies have different strengths and weaknesses, however they all share a common problem,...

ER-based double iCRE fusion protein allows partial recombination in forebrain (2002)

Casanova, James E., Fehsenfeld, Sandra, Lemberger, Thomas, Shimshek, Derya R., Sprengel, Rolf, Mantamadiotis, Theo

Here we describe the generation of a new tamoxifen-inducible double Cre fusion protein generated by fusing two ERT2 domains onto both ends of the iCre recombinase (a codon improved Cre recombinase)....

Molecular dissection of hippocampal theta-burst pairing potentiation (2002)

Hoffman, Dax A., Sprengel, Rolf, Sakmann, Bert

Long-term potentiation (LTP) of synaptic efficacy in the hippocampus is frequently induced by tetanic stimulation of presynaptic afferents or by pairing low frequency stimulation with postsynaptic...

Deletion of the C-terminal domain of the NR2B subunit alters channel properties and synaptic targeting of NMDA receptors in nascent neocortical synapses (2002)

Mohrmann, Ralf, Köhr, Georg, Sprengel, Rolf, Hatt, Hanns, Gottmann, Kurt

Channel properties and synaptic targeting of N-methyl-D-aspartate (NMDA) receptors determine their importance in synaptic transmission, long-term synaptic plasticity, and developmental reorganization...

Codon-improved Cre recombinase (iCre) expression in the mouse (2002)

Shimshek, Derya R., Kim, Jin-Hyun, Hübner, Michael R., Spergel, Daniel J., Buchholz, Frank, Casanova, James E., ...

By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre...

Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive NR1(N598R) expression (2001)

Jerecic,Jasna, Schulze, Christian H., Seeburg,Peter H., Jonas,Peter, Sprengel,Rolf, Bischofsberger,Josef

High Ca2+ permeability and its control by voltage-dependent Mg2+ block are defining features of NMDA receptors. These features are lost if the principal NR1 subunit carries an asparagine (N) to...

Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive NR1(N598R) expression (2001)

Jerecic,Jasna, Schulze, Christian H., Seeburg,Peter H., Jonas,Peter, Sprengel,Rolf, Bischofsberger,Josef

High Ca2+ permeability and its control by voltage-dependent Mg2+ block are defining features of NMDA receptors. These features are lost if the principal NR1 subunit carries an asparagine (N) to...

Genetic manipulation of key determinants of ion flow in glutamate receptor channels in the mouse (2001)

Seeburg,Peter H., Single,Frank N., Kuner,Thomas, Higuchi,Miyoko, Sprengel,Rolf

Glutamate receptor channels are built around an ancient pore loop structure which defines the inner channel environment and which is connected to structures for channel gating. This pore loop, which...

Genetic manipulation of key determinants of ion flow in glutamate receptor channels in the mouse (2001)

Seeburg,Peter H., Single,Frank N., Kuner,Thomas, Higuchi,Miyoko, Sprengel,Rolf

Glutamate receptor channels are built around an ancient pore loop structure which defines the inner channel environment and which is connected to structures for channel gating. This pore loop, which...

Conditional restoration of hippocampal synaptic potentiation in GluR-A-deficient mice (2001)

Mack,Volker, Burnashev,Nail, Kaiser,Katharina M. M., Rozov,Andrej, Jensen,Vidar, Hvalby,Øivind, ...

Plasticity of mature hippocampal CA1 synapses is dependent on L-alpha -amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors containing the glutamate receptor A (GluR-A) subunit. In...

Conditional restoration of hippocampal synaptic potentiation in GluR-A-deficient mice (2001)

Mack,Volker, Burnashev,Nail, Kaiser,Katharina M. M., Rozov,Andrej, Jensen,Vidar, Hvalby,Øivind, ...

Plasticity of mature hippocampal CA1 synapses is dependent on L-alpha -amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors containing the glutamate receptor A (GluR-A) subunit. In...

Morphine-induced dependence and sensitization are altered in mice deficient in AMPA-type glutamate receptor-A subunits (2001)

Vekovischeva,Olga Y., Zamanillo,Daniel, Echenko,Oxana, Seppälä,Timo, Uusi-Oukari,Mikko, Honkanen,Aapo, ...

AMPA-type glutamate receptors have been suggested to be involved in the neurobiological mechanisms of drug addiction. We have made use of two mouse lines, which both have modulated AMPA receptor...

Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies (2001)

Spergel,Daniel J., Krüth,Ulrich, Shimshek,Derya R., Sprengel,Rolf, Seeburg,Peter H.

This review summarizes recent work on the use of reporter genes to label selected neuronal populations in transgenic mice, with particular emphasis on gonadotropin-releasing hormone (GnRH) neurons....

A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse (2001)

Krestel,Heinz Eric, Mayford,Mark, Seeburg,Peter H., Sprengel,Rolf

Doxycycline (Dox)-sensitive co-regulation of two transcriptionally coupled transgenes was investigated in the mouse. For this, we generated four independent mouse lines carrying coding regions for...

Glutamate receptor channel signatures (2001)

Sprengel,Rolf, Aronoff,Rachel, Völkner,Michaela, Schmitt,Bertram, Mosbach,Raphael, Kuner,Thomas

Genes encoding glutamate receptor channel subunits were identified in genomes from Drosophila melanogaster and Caenorhabditis elegans by homology search with amino acid sequences that participate in...

Glutamate receptor channel signatures (2001)

Sprengel,Rolf, Aronoff,Rachel, Völkner,Michaela, Schmitt,Bertram, Mosbach,Raphael, Kuner,Thomas

Genes encoding glutamate receptor channel subunits were identified in genomes from Drosophila melanogaster and Caenorhabditis elegans by homology search with amino acid sequences that participate in...

Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive NR1(N598R) expression (2001)

Jerecic, Jasna, Schulze, Christian H., Seeburg, Peter H., Jonas, Peter, Sprengel, Rolf, Bischofsberger, Josef

High Ca2+ permeability and its control by voltage-dependent Mg2+ block are defining features of NMDA receptors. These features are lost if the principal NR1 subunit carries an asparagine (N) to...

A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse (2001)

Krestel, Heinz Eric, Mayford, Mark, Seeburg, Peter H., Sprengel, Rolf

Doxycycline (Dox)-sensitive co-regulation of two transcriptionally coupled transgenes was investigated in the mouse. For this, we generated four independent mouse lines carrying coding regions for...

Conditional restoration of hippocampal synaptic potentiation in GluR-A-deficient mice (2001)

Mack, Volker, Burnashev, Nail, Kaiser, Katharina M. M., Rozov, Andrej, Jensen, Vidar, Hvalby, Øivind, ...

Plasticity of mature hippocampal CA1 synapses is dependent on L-alpha -amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors containing the glutamate receptor A (GluR-A) subunit. In...

Genetic manipulation of key determinants of ion flow in glutamate receptor channels in the mouse (2001)

Seeburg, Peter H., Single, Frank N., Kuner, Thomas, Higuchi, Miyoko, Sprengel, Rolf

Glutamate receptor channels are built around an ancient pore loop structure which defines the inner channel environment and which is connected to structures for channel gating. This pore loop, which...

Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies (2001)

Spergel, Daniel J., Krüth, Ulrich, Shimshek, Derya R., Sprengel, Rolf, Seeburg, Peter H.

This review summarizes recent work on the use of reporter genes to label selected neuronal populations in transgenic mice, with particular emphasis on gonadotropin-releasing hormone (GnRH) neurons....

Glutamate receptor channel signatures (2001)

Sprengel, Rolf, Aronoff, Rachel, Völkner, Michaela, Schmitt, Bertram, Mosbach, Raphael, Kuner, Thomas

Genes encoding glutamate receptor channel subunits were identified in genomes from Drosophila melanogaster and Caenorhabditis elegans by homology search with amino acid sequences that participate in...

Morphine-induced dependence and sensitization are altered in mice deficient in AMPA-type glutamate receptor-A subunits (2001)

Vekovischeva, Olga Y., Zamanillo, Daniel, Echenko, Oxana, Seppälä, Timo, Uusi-Oukari, Mikko, Honkanen, Aapo, ...

AMPA-type glutamate receptors have been suggested to be involved in the neurobiological mechanisms of drug addiction. We have made use of two mouse lines, which both have modulated AMPA receptor...

Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive NR1(N598R) expression (2001)

Jerecic, Jasna, Schulze, Christian H., Seeburg, Peter H., Jonas, Peter, Sprengel, Rolf, Bischofsberger, Josef

High Ca2+ permeability and its control by voltage-dependent Mg2+ block are defining features of NMDA receptors. These features are lost if the principal NR1 subunit carries an asparagine (N) to...

Conditional restoration of hippocampal synaptic potentiation in GluR-A-deficient mice (2001)

Mack, Volker, Burnashev, Nail, Kaiser, Katharina M. M., Rozov, Andrej, Jensen, Vidar, Hvalby, Øivind, ...

Plasticity of mature hippocampal CA1 synapses is dependent on L-alpha -amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors containing the glutamate receptor A (GluR-A) subunit. In...

Genetic manipulation of key determinants of ion flow in glutamate receptor channels in the mouse (2001)

Seeburg, Peter H., Single, Frank N., Kuner, Thomas, Higuchi, Miyoko, Sprengel, Rolf

Glutamate receptor channels are built around an ancient pore loop structure which defines the inner channel environment and which is connected to structures for channel gating. This pore loop, which...

Glutamate receptor channel signatures (2001)

Sprengel, Rolf, Aronoff, Rachel, Völkner, Michaela, Schmitt, Bertram, Mosbach, Raphael, Kuner, Thomas

Genes encoding glutamate receptor channel subunits were identified in genomes from Drosophila melanogaster and Caenorhabditis elegans by homology search with amino acid sequences that participate in...

Respiration and parturition affected by conditional overexpression of the Ca2+ -activated K+ channel subunit, SK3 (2000)

Bond,Chris T., Sprengel,Rolf, Bissonnette,John M., Kaufmann,Walter A., Pribnow,David, Neelands,Torben, ...

In excitable cells, small-conductance Ca2+-activated potassium channels (SK channels) are responsible for the slow after-hyperpolarization that often follows an action potential. Three SK channel...

Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2 (2000)

Higuchi,Miyoko, Maas,Stefan, Single,Frank N., Hartner,Jochen C., Rozov,Andrej, Burnashev,Nail, ...

RNA editing by site-selective deamination of adenosine to inosine1, 2 alters codons3, 4 and splicing5 in nuclear transcripts6, and therefore protein function. ADAR2 (refs 7, 8) is a candidate...

Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2 (2000)

Higuchi,Miyoko, Maas,Stefan, Single,Frank N., Hartner,Jochen C., Rozov,Andrej, Burnashev,Nail, ...

RNA editing by site-selective deamination of adenosine to inosine1, 2 alters codons3, 4 and splicing5 in nuclear transcripts6, and therefore protein function. ADAR2 (refs 7, 8) is a candidate...

Dysfunction in mice by NMDA receptor point mutations NR1(N598Q) and NR1(N598R) (2000)

Single,Frank N., Rozov,Andrej, Burnashev,Nail, Zimmermann,Frank, Hanley,Daniel F., Forrest,Douglas, ...

NMDA receptors in mice were mutated by gene targeting to substitute asparagine (N) in position 598 of the NR1 subunit to glutamine (Q) or arginine (R). Animals expressing exclusively the mutated NR1...

Dysfunction in mice by NMDA receptor point mutations NR1(N598Q) and NR1(N598R) (2000)

Single,Frank N., Rozov,Andrej, Burnashev,Nail, Zimmermann,Frank, Hanley,Daniel F., Forrest,Douglas, ...

NMDA receptors in mice were mutated by gene targeting to substitute asparagine (N) in position 598 of the NR1 subunit to glutamine (Q) or arginine (R). Animals expressing exclusively the mutated NR1...

Respiration and parturition affected by conditional overexpression of the Ca2+ -activated K+ channel subunit, SK3 (2000)

Bond, Chris T., Sprengel, Rolf, Bissonnette, John M., Kaufmann, Walter A., Pribnow, David, Neelands, Torben, ...

In excitable cells, small-conductance Ca2+-activated potassium channels (SK channels) are responsible for the slow after-hyperpolarization that often follows an action potential. Three SK channel...

Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2 (2000)

Higuchi, Miyoko, Maas, Stefan, Single, Frank N., Hartner, Jochen C., Rozov, Andrej, Burnashev, Nail, ...

RNA editing by site-selective deamination of adenosine to inosine1, 2 alters codons3, 4 and splicing5 in nuclear transcripts6, and therefore protein function. ADAR2 (refs 7, 8) is a candidate...

Dysfunction in mice by NMDA receptor point mutations NR1(N598Q) and NR1(N598R) (2000)

Single, Frank N., Rozov, Andrej, Burnashev, Nail, Zimmermann, Frank, Hanley, Daniel F., Forrest, Douglas, ...

NMDA receptors in mice were mutated by gene targeting to substitute asparagine (N) in position 598 of the NR1 subunit to glutamine (Q) or arginine (R). Animals expressing exclusively the mutated NR1...

Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2 (2000)

Higuchi, Miyoko, Maas, Stefan, Single, Frank N., Hartner, Jochen C., Rozov, Andrej, Burnashev, Nail, ...

RNA editing by site-selective deamination of adenosine to inosine1, 2 alters codons3, 4 and splicing5 in nuclear transcripts6, and therefore protein function. ADAR2 (refs 7, 8) is a candidate...

Dysfunction in mice by NMDA receptor point mutations NR1(N598Q) and NR1(N598R) (2000)

Single, Frank N., Rozov, Andrej, Burnashev, Nail, Zimmermann, Frank, Hanley, Daniel F., Forrest, Douglas, ...

NMDA receptors in mice were mutated by gene targeting to substitute asparagine (N) in position 598 of the NR1 subunit to glutamine (Q) or arginine (R). Animals expressing exclusively the mutated NR1...

Neurological dysfunctions in mice expressing different levels of the Q/R site-unedited AMPAR subunit GluR-B (1999)

Feldmeyer,Dirk, Kask,Kalev, Brusa,Rossella, Kornau,Hans Christian, Kolhekar,Rohini, Rozov,Andrej, ...

We generated mouse mutants with targeted AMPA receptor (AMPAR) GluR–B subunit alleles, functionally expressed at different levels and deficient in Q/R–site editing. All mutant lines had increased...

Neurological dysfunctions in mice expressing different levels of the Q/R site-unedited AMPAR subunit GluR-B (1999)

Feldmeyer,Dirk, Kask,Kalev, Brusa,Rossella, Kornau,Hans Christian, Kolhekar,Rohini, Rozov,Andrej, ...

We generated mouse mutants with targeted AMPA receptor (AMPAR) GluR–B subunit alleles, functionally expressed at different levels and deficient in Q/R–site editing. All mutant lines had increased...

Neurological dysfunctions in mice expressing different levels of the Q/R site-unedited AMPAR subunit GluR-B (1999)

Feldmeyer, Dirk, Kask, Kalev, Brusa, Rossella, Kornau, Hans Christian, Kolhekar, Rohini, Rozov, Andrej, ...

We generated mouse mutants with targeted AMPA receptor (AMPAR) GluR–B subunit alleles, functionally expressed at different levels and deficient in Q/R–site editing. All mutant lines had increased...

Neurological dysfunctions in mice expressing different levels of the Q/R site-unedited AMPAR subunit GluR-B (1999)

Feldmeyer, Dirk, Kask, Kalev, Brusa, Rossella, Kornau, Hans Christian, Kolhekar, Rohini, Rozov, Andrej, ...

We generated mouse mutants with targeted AMPA receptor (AMPAR) GluR–B subunit alleles, functionally expressed at different levels and deficient in Q/R–site editing. All mutant lines had increased...

The AMPA receptor subunit GluR-B in its Q/R site-unedited form is not essential for brain development and function (1998)

Kask,Kalev, Zamanillo,Daniel, Rozov,Andrej, Burnashev,Nail, Sprengel,Rolf, Seeburg,Peter H.

Calcium permeability of L--amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) in excitatory neurons of the mammalian brain is prevented by coassembly of the GluR-B subunit, which...

The AMPA receptor subunit GluR-B in its Q/R site-unedited form is not essential for brain development and function (1998)

Kask,Kalev, Zamanillo,Daniel, Rozov,Andrej, Burnashev,Nail, Sprengel,Rolf, Seeburg,Peter H.

Calcium permeability of L--amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) in excitatory neurons of the mammalian brain is prevented by coassembly of the GluR-B subunit, which...

Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo (1998)

Sprengel,Rolf, Suchanek,Bettina, Amico,Carla, Brusa,Rossella, Burnashev,Nail, Rozov,Andrej, ...

NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity of central excitatory synapses. We show here that gene-targeted mice...

Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo (1998)

Sprengel,Rolf, Suchanek,Bettina, Amico,Carla, Brusa,Rossella, Burnashev,Nail, Rozov,Andrej, ...

NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity of central excitatory synapses. We show here that gene-targeted mice...

The AMPA receptor subunit GluR-B in its Q/R site-unedited form is not essential for brain development and function (1998)

Kask, Kalev, Zamanillo, Daniel, Rozov, Andrej, Burnashev, Nail, Sprengel, Rolf, Seeburg, Peter H.

Calcium permeability of L--amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) in excitatory neurons of the mammalian brain is prevented by coassembly of the GluR-B subunit, which...

Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo (1998)

Sprengel, Rolf, Suchanek, Bettina, Amico, Carla, Brusa, Rossella, Burnashev, Nail, Rozov, Andrej, ...

NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity of central excitatory synapses. We show here that gene-targeted mice...

The AMPA receptor subunit GluR-B in its Q/R site-unedited form is not essential for brain development and function (1998)

Kask, Kalev, Zamanillo, Daniel, Rozov, Andrej, Burnashev, Nail, Sprengel, Rolf, Seeburg, Peter H.

Calcium permeability of L--amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) in excitatory neurons of the mammalian brain is prevented by coassembly of the GluR-B subunit, which...

Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo (1998)

Sprengel, Rolf, Suchanek, Bettina, Amico, Carla, Brusa, Rossella, Burnashev, Nail, Rozov, Andrej, ...

NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity of central excitatory synapses. We show here that gene-targeted mice...

RED2, a brain specific member of the RNA specific adenosine deaminase family (1996)

Melcher,Thorsten, Maas,Stefan, Herb,Anne, Sprengel,Rolf, Higuchi,Miyoko, Seeburg,Peter H.

The mammalian RNA-specific adenosine deaminases DRADA/dsRAD (alias ADAR) and RED1 (alias ADARB1) have been implicated in the site-selective editing of brain-expressed pre-mRNAs for glutamate receptor...

Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences (1996)

Herb,Anne, Higuchi,Miyoko, Sprengel,Rolf, Seeburg,Peter H.

RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channels Q/R...

Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse (1996)

Storck,Thorsten, Storck,U., Kolhekar,Rohini, Sprengel,Rolf, Seeburg,Peter H.

Targeting vectors for embryonic stem (ES) cells typically contain a mouse gene segment of >7 kb with the neo gene inserted for positive selection of the targeting event. More complex targeting...

Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences (1996)

Herb, Anne, Higuchi, Miyoko, Sprengel, Rolf, Seeburg, Peter H.

RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channels Q/R...

RED2, a brain specific member of the RNA specific adenosine deaminase family (1996)

Melcher, Thorsten, Maas, Stefan, Herb, Anne, Sprengel, Rolf, Higuchi, Miyoko, Seeburg, Peter H.

The mammalian RNA-specific adenosine deaminases DRADA/dsRAD (alias ADAR) and RED1 (alias ADARB1) have been implicated in the site-selective editing of brain-expressed pre-mRNAs for glutamate receptor...

Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse (1996)

Storck, Thorsten, Storck, U., Kolhekar, Rohini, Sprengel, Rolf, Seeburg, Peter H.

Targeting vectors for embryonic stem (ES) cells typically contain a mouse gene segment of >7 kb with the neo gene inserted for positive selection of the targeting event. More complex targeting...

Early-onset epilepsy and postnatal lethality associated with an editing-deficient GluR-B allele in mice (1995)

Brusa,Rossella, Zimmermann,Frank, Feldmeyer,Dirk, Gass,Peter, Seeburg,Peter H., ...

The arginine residue at position 586 of the GluR-B subunit renders heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-sensitive glutamate receptor channels impermeable to...

Early-onset epilepsy and postnatal lethality associated with an editing-deficient GluR-B allele in mice (1995)

Brusa,Rossella, Zimmermann,Frank, Feldmeyer,Dirk, Gass,Peter, Seeburg,Peter H., ...

The arginine residue at position 586 of the GluR-B subunit renders heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-sensitive glutamate receptor channels impermeable to...

Gene Structure of the Murine N-MethylD-Aspartate Receptor Subunit NR2C (1995)

Suchanek,Bettina, Seeburg,Peter H., Sprengel,Rolf

The murine N-methyl D-aspartate receptor subunit NR2C (-3) is encoded by a unique gene composed of 12 translated and three 5`-untranslated exons that spread over ˜20 kilobases of genomic sequence....

Early-onset epilepsy and postnatal lethality associated with an editing-deficient GluR-B allele in mice (1995)

Brusa, Rossella, Zimmermann, Frank, Feldmeyer, Dirk, Gass, Peter, Seeburg, Peter H., ...

The arginine residue at position 586 of the GluR-B subunit renders heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-sensitive glutamate receptor channels impermeable to...

Gene Structure of the Murine N-MethylD-Aspartate Receptor Subunit NR2C (1995)

Suchanek, Bettina, Seeburg, Peter H., Sprengel, Rolf

The murine N-methyl D-aspartate receptor subunit NR2C (-3) is encoded by a unique gene composed of 12 translated and three 5`-untranslated exons that spread over ˜20 kilobases of genomic sequence....

Early-onset epilepsy and postnatal lethality associated with an editing-deficient GluR-B allele in mice (1995)

Brusa, Rossella, Zimmermann, Frank, Feldmeyer, Dirk, Gass, Peter, Seeburg, Peter H., ...

The arginine residue at position 586 of the GluR-B subunit renders heteromeric alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-sensitive glutamate receptor channels impermeable to...

RNA editing of AMPA receptor subunit GluR-B: a base-paired intron exon structure determine position and efficiency (1993)

Higuchi,Miyoko, Single,Frank N., Köhler,Martin, Sommer,B., Sprengel,Rolf, Seeburg,Peter H.

A functionally critical position (Q/R site) of the AMPA receptor subunit GluR-B is controlled by RNA editing that operates in the nucleus, since in brain and clonal cell lines of neural origin,...

The unique properties of glutamate receptor channels (1993)

Sprengel,Rolf, Seeburg,Peter H.

Rapid excitatory neurotransmission in the central nervous system (CNS) is mediated predominantly by synaptically released -glutamate which activates cation selective channels with different kinetic...

The rat delta-1 and delta-2 subunits extend the excitatory amino acid receptor family (1993)

Lomeli,H., Sprengel,Rolf, Laurie,David J., Köhr,Georg, Herb,Anne, Seeburg,Peter H., ...

We have characterized a second member (delta-2) of a new class of subunits for the ligand-gated excitatory amino acid receptor superfamily. The sequence of delta-2 exhibits an average identity of 25%...

Structure functional expression and spatial distribution of a cloned cDNA encoding a rat 5-HT1D-like receptor (1993)

Bach,A. W. J., Unger,L., Sprengel,Rolf, Mengod,G., Palacios,J., Seeburg,Peter H., ...

Using polymerase chain reaction (PCR) a complementary DNA (cDNA) encoding a 5-hydroxytryptamine (5-HT) receptor was isolated from rat forebrain. The amplified cDNA specifies an open reading frame of...

Structure functional expression and spatial distribution of a cloned cDNA encoding a rat 5-HT1D-like receptor (1993)

Bach, A. W. J., Unger, L., Sprengel, Rolf, Mengod, G., Palacios, J., Seeburg, Peter H., ...

Using polymerase chain reaction (PCR) a complementary DNA (cDNA) encoding a 5-hydroxytryptamine (5-HT) receptor was isolated from rat forebrain. The amplified cDNA specifies an open reading frame of...

RNA editing of AMPA receptor subunit GluR-B: a base-paired intron exon structure determine position and efficiency (1993)

Higuchi, Miyoko, Single, Frank N., Köhler, Martin, Sommer, B., Sprengel, Rolf, Seeburg, Peter H.

A functionally critical position (Q/R site) of the AMPA receptor subunit GluR-B is controlled by RNA editing that operates in the nucleus, since in brain and clonal cell lines of neural origin,...

The rat delta-1 and delta-2 subunits extend the excitatory amino acid receptor family (1993)

Lomeli, H., Sprengel, Rolf, Laurie, David J., Köhr, Georg, Herb, Anne, Seeburg, Peter H., ...

We have characterized a second member (delta-2) of a new class of subunits for the ligand-gated excitatory amino acid receptor superfamily. The sequence of delta-2 exhibits an average identity of 25%...

The unique properties of glutamate receptor channels (1993)

Sprengel, Rolf, Seeburg, Peter H.

Rapid excitatory neurotransmission in the central nervous system (CNS) is mediated predominantly by synaptically released -glutamate which activates cation selective channels with different kinetic...

Translationally coupled initiation of protein synthesis in Bacillus subtilis (1985)

Sprengel, Rolf, Reiss, Bernd, Schaller, Heinz

The neomycin phosphotransferase gene (neo) from Transposon Tn5 is active in Gram-negative bacteria but silent in B.subtilis since it lacks an appropriate ribosome binding site for Gram-positive...

Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium (1981)

Neugebauer, Kristina, Sprengel, Rolf, Schaller, Heinz

The gene for the penicillinase from B. licheniformis has been cloned in a functional state on a 1.5 kb DNA fragment and its nucleotide sequence has been determined. A sequence of 307 amino acid...

The AMPA receptor subunit GluR-B in its Q/R site-unedited form is not essential for brain development and function

Kask, Kalev, Zamanillo, Daniel, Rozov, Andrei, Burnashev, Nail, Sprengel, Rolf, Seeburg, Peter H.

Calcium permeability of l-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) in excitatory neurons of the mammalian brain is prevented by coassembly of the GluR-B subunit, which...

Enhanced Odor Discrimination and Impaired Olfactory Memory by Spatially Controlled Switch of AMPA Receptors

Shimshek, Derya R, Bus, Thorsten, Kim, Jinhyun, Mihaljevic, Andre, Mack, Volker, Seeburg, Peter H, ...

Genetic perturbations of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) are widely used to dissect molecular mechanisms of sensory coding, learning, and memory. In this study,...

The AMPA receptor subunit GluR-B in its Q/R site-unedited form is not essential for brain development and function

Kask, Kalev, Zamanillo, Daniel, Rozov, Andrei, Burnashev, Nail, Sprengel, Rolf, Seeburg, Peter H.

Calcium permeability of l-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) in excitatory neurons of the mammalian brain is prevented by coassembly of the GluR-B subunit, which...

Enhanced Odor Discrimination and Impaired Olfactory Memory by Spatially Controlled Switch of AMPA Receptors

Shimshek, Derya R, Bus, Thorsten, Kim, Jinhyun, Mihaljevic, Andre, Mack, Volker, Seeburg, Peter H, ...

Genetic perturbations of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) are widely used to dissect molecular mechanisms of sensory coding, learning, and memory. In this study,...

Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons

Zhu, Peixin, Aller, M. Isabel, Baron, Udo, Cambridge, Sidney, Bausen, Melanie, Herb, Jan, ...

To identify the underlying reason for the controversial performance of tetracycline (Tet)-controlled regulated gene expression in mammalian neurons, we investigated each of the three components that...

Reduced cortical inhibition in a mouse model of familial childhood absence epilepsy

Tan, Heneu O., Reid, Christopher A., Single, Frank N., Davies, Philip J., Chiu, Cindy, Murphy, Susan, ...

Mutations in the GABAA receptor γ2 subunit are associated with childhood absence epilepsy and febrile seizures. To understand better the molecular basis of absence epilepsy in man, we developed a...

Impaired Regulation of Synaptic Strength in Hippocampal Neurons from GluR1-Deficient Mice

Andrásfalvy, Bertalan K, Smith, Mark A, Borchardt, Thilo, Sprengel, Rolf, Magee, Jeffrey C

Neurons of the central nervous system (CNS) exhibit a variety of forms of synaptic plasticity, including associative long-term potentiation and depression (LTP/D), homeostatic activity-dependent...

A juvenile form of postsynaptic hippocampal long-term potentiation in mice deficient for the AMPA receptor subunit GluR-A

Jensen, Vidar, Kaiser, Katharina M M, Borchardt, Thilo, Adelmann, Giselind, Rozov, Andrei, Burnashev, Nail, ...

In adult mice, long-term potentiation (LTP) of synaptic transmission at CA3-to-CA1 synapses induced by tetanic stimulation requires l-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)...

Glutamate mediates platelet activation through the AMPA receptor

Morrell, Craig N., Sun, Henry, Ikeda, Masahiro, Beique, Jean-Claude, Swaim, Anne Marie, Mason, Emily, ...

Glutamate is an excitatory neurotransmitter that binds to the kainate receptor, the N-methyl-D-aspartate (NMDA) receptor, and the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor...

Impaired Associative Fear Learning in Mice with Complete Loss or Haploinsufficiency of AMPA GluR1 Receptors

Feyder, Michael, Wiedholz, Lisa, Sprengel, Rolf, Holmes, Andrew

There is compelling evidence that l-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) glutamate receptors containing the GluR1 subunit contribute to the molecular mechanisms associated with...

Split-Cre Complementation Indicates Coincident Activity of Different Genes In Vivo

Hirrlinger, Johannes, Scheller, Anja, Hirrlinger, Petra G., Kellert, Beate, Tang, Wannan, Wehr, Michael C., ...

Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell...

Induction and expression of GluA1 (GluR-A)-independent LTP in the hippocampus

Romberg, Carola, Raffel, Joel, Martin, Lucy, Sprengel, Rolf, Seeburg, Peter H, Rawlins, J Nicholas P, ...

Long-term potentiation (LTP) at hippocampal CA3–CA1 synapses is thought to be mediated, at least in part, by an increase in the postsynaptic surface expression of...

Enhanced long-term and impaired short-term spatial memory in GluA1 AMPA receptor subunit knockout mice: Evidence for a dual-process memory model

Sanderson, David J., Good, Mark A., Skelton, Kathryn, Sprengel, Rolf, Seeburg, Peter H., Rawlins, J. Nicholas P., ...

The GluA1 AMPA receptor subunit is a key mediator of hippocampal synaptic plasticity and is especially important for a rapidly-induced, short-lasting form of potentiation. GluA1 gene deletion impairs...

Roles of the AMPA receptor subunit GluA1 but not GluA2 in synaptic potentiation and activation of ERK in the anterior cingulate cortex

Toyoda, Hiroki, Zhao, Ming-Gao, Ulzhöfer, Bettina, Wu, Long-Jun, Xu, Hui, Seeburg, Peter H, ...

Cortical areas including the anterior cingulate cortex (ACC) are important for pain and pleasure. Recent studies using genetic and physiological approaches have demonstrated that the investigation of...