Mohapatra, Shyam S., Poole, Ronald J., Dhindsa, Rajinder S.
Membrane proteins labeled in vivo from cold-acclimated and ABA-treated alfalfa seedlings of two cultivars differing in cold-tolerance have been compared by SDS polyacrylamide gel electrophoresis and...
MOHAPATRA, SHYAM S., POOLE, RONALD J., DHINDSA, RAJINDER S.
Mohapatra, S. S., Poole, R. J. and Dhindsa, R. S. 1987. Cold acclimation, freezing resistance and protein synthesis in alfalfa (Medicago sativa L. cv. Saranac).—J. exp. Bot. 38: 1697–1703....
Gerson, Donald F., Poole, Ronald J.
Net uptake of Cl− into root tips of mung bean (Phaseolus aureus) increases steadily with increasing external concentrations from 1 to 60 mm. Membrane potentials were measured to determine the...
Carrier-mediated Potassium Efflux Across the Cell Membrane of Red Beet
The flux ratio (influx/efflux) of K+ across the plasmalemma of beet cells at an external potassium concentration of 0.6 mm does not respond to changes of membrane potential in the manner expected for...
Effect of Sodium on Potassium Fluxes at the Cell Membrane and Vacuole Membrane of Red Beet 1
Slices of red beet (Beta vulgaris) washed for 5 to 6 days are known to accumulate Na+ in preference to K+ from solutions containing both ions. The present work, using ion concentrations of 1.0 mm or...
Development and Characteristics of Sodium-selective Transport in Red Beet 1
Slices of storage tissue of red beet (Beta vulgaris L.) washed for only 1 day in distilled water readily absorb K+ but lack a mechanism for rapid Na+ uptake. A Na+ transport mechanism develops if the...
Blumwald, Eduardo, Poole, Ronald J.
The fluorescent probes acridine orange and oxonol-V were used as indicators of pH gradients (ΔpH) and membrane potential differences (ΔΨ), respectively, in membrane vesicles believed to be derived...
ATP Levels and their Effects on Plasmalemma Influxes of Potassium Chloride in Red Beet 1
Petraglia, Teresa, Poole, Ronald J.
Tissue ATP concentrations in slices of red beet increase progressively with time for up to 7 days after cutting the root. ATP levels are higher in slices taken from stored roots than in slices from...
Effect of Anoxia on ATP Levels and Ion Transport Rates in Red Beet 1
Petraglia, Teresa, Poole, Ronald J.
The ATP content of disks of storage tissue of red beet is reduced under N2 atmosphere to between 10 and 25% of its value in air. Plasmalemma fluxes of K+ and Cl− are inhibited within 1 minute or...
Isolation of Protoplasts and Vacuoles from Storage Tissue of Red Beet 1
Schmidt, Rainer, Poole, Ronald J.
A fast and efficient method is described for the isolation of protoplasts and vacuoles from storage tissue of Beta vulgaris L. The viability of the isolated protoplasts is indicated by the...
Regulation of Cytoplasmic and Vacuolar Volumes by Plant Cells in Suspension Culture 1
Owens, Trevor, Poole, Ronald J.
Quantitative microscopical measurements have been made of the proportion of cell volume occupied by cytoplasm in a cell suspension culture derived from cotyledons of bush bean (cv. Contender). On a...
Inhibition of Na+/H+ Antiport Activity in Sugar Beet Tonoplast by Analogs of Amiloride 1
Blumwald, Eduardo, Cragoe, Edward J., Poole, Ronald J.
The effects of amiloride and a series of amiloride analogs have been tested on the Na+/H+ antiport activity in intact vacuoles and tonoplast vesicles isolated from sugar beet cell suspension...
Alterations in Membrane Protein-Profile during Cold Treatment of Alfalfa 1
Mohapatra, Shyam S., Poole, Ronald J., Dhindsa, Rajinder S.
Changes in pattern of membrane proteins during cold acclimation of alfalfa have been examined. Cold acclimation for 2 to 3 days increases membrane protein content. Labeling of membrane proteins in...
Abscisic Acid-Regulated Gene Expression in Relation to Freezing Tolerance in Alfalfa 1
Mohapatra, Shyam S., Poole, Ronald J., Dhindsa, Rajinder S.
A comparison of abscisic acid (ABA)-induced and cold-acclimation-induced freezing tolerance in two alfalfa cultivars (Medicago falcata cv Anik and Medicago sativa v Trek) indicates that ABA alone can...
Mohapatra, Shyam S., Wolfraim, Lawrence, Poole, Ronald J., Dhindsa, Rajinder S.
Cold-acclimation-specific (CAS) gene expression has been examined by screening a cDNA library prepared from poly(A)+ RNA of cold-acclimated seedlings of a freezing-tolerant variety of alfalfa...
Characterization of Potassium-Dependent Currents in Protoplasts of Corn Suspension Cells 1
Ketchum, Karen A., Shrier, Alvin, Poole, Ronald J.
Protoplasts obtained from corn (Zea mays) suspension cells were studied using the whole cell patch-clamp technique. One time-independent current, as well as two time-dependent currents were...
Blumwald, Eduardo, Poole, Ronald J.
Cell suspension cultures of sugar beet were grown at various salinities (0-200 millimolar NaCl). Their tolerance to Na+ was comparable to that of the intact plant. Tonoplast vesicles were prepared by...
Mohapatra, Shyam S., Poole, Ronald J., Dhindsa, Rajinder S.
Changes in the rate and pattern of protein synthesis and in translatable mRNA population during cold acclimation of alfalfa (Medicago falcata cv Anik) seedlings have been examined. There appears to...
Purification of an H+-Translocating Inorganic Pyrophosphatase from Vacuole Membranes of Red Beet 1
Sarafian, Vahé, Poole, Ronald J.
An H+-translocating inorganic pyrophosphatase (PPase) was isolated and purified from red beet (Beta vulgaris L.) tonoplast. One major polypeptide of molecular weight 67 kilodalton copurified with...
Characterization of the Solubilized Plasma Membrane ATPase of Red Beet 1
Briskin, Donald P., Poole, Ronald J.
The plasma membrane ATP-phosphohydrolase (ATPase) from red beet (Beta vulgaris L.) storage tissue was solubilized with the zwitterionic detergent Zwittergent 3-14 from a plasma membrane-enriched...
Proton-Translocating Inorganic Pyrophosphatase in Red Beet (Beta vulgaris L.) Tonoplast Vesicles 1
Rea, Philip A., Poole, Ronald J.
The substrate and ionic requirements of ATP and inorganic pyrophosphate (PPi) hydrolysis by tonoplast vesicles isolated from storage tissue of red beet (Beta vulgaris L.) were compared with the...
Na+/H+ Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris1
Blumwald, Eduardo, Poole, Ronald J.
The pH-dependent fluorescence quenching of acridine orange was used to study the Na+- and K+-dependent H+ fluxes in tonoplast vesicles isolated from storage tissue of red beet and sugar beet (Beta...
Blumwald, Eduardo, Fortin, Marc G., Rea, Philip A., Verma, Desh Pal S., Poole, Ronald J.
An improved method is described for the isolation of membrane envelope enclosing the bacteroids (peribacteroid membrane) from soybean (Glycine max L.) root nodules. The ATPase activity of the...
Briskin, Donald P., Poole, Ronald J.
A membrane fraction enriched with a magnesium-dependent, monovalent cation-stimulated ATPase was isolated from red beet (Beta vulgaris L.) storage roots by a combination of differential...
Plasma Membrane ATPase of Red Beet Forms a Phosphorylated Intermediate 1
Briskin, Donald P., Poole, Ronald J.
When a plasma membrane-enriched fraction isolated from red beet (Beta vulgaris L.) was incubated in the presence of 40 micromolar [γ-32P] ATP, 40 micromolar MgSO4 at pH 6.5, a rapidly turning over...
Role of Magnesium in the Plasma Membrane ATPase of Red Beet 1
Briskin, Donald P., Poole, Ronald J.
The phosphorylation technique was used to assess the role of Mg in the red beet (Beta vulgaris L.) plasma membrane ATPase. When an excess of ethylenediaminetetraacetate (Tris salt, pH 6.5) was added...
Briskin, Donald P., Poole, Ronald J.
A borohydride reduction method was used to identify the phosphorylated amino acid in the phospho-enzyme of the red beet (Beta vulgaris L.) plasma membrane ATPase. Plasma membrane fractions were...
Poole, Ronald J., Briskin, Donald P., Krátký, Zdeněk, Johnstone, Rose M.
Membranes from homogenates of growing and of dormant storage roots of red beet (Beta vulgaris L.) were centrifuged on linear sucrose gradients. Vanadate-sensitive ATPase activity, a marker for plasma...
Blumwald, Eduardo, Poole, Ronald J.
Artificial pH gradients across tonoplast vesicles isolated from storage tissue of red beet (Beta vulgaris L.) were used to study the kinetics of a Ca2+/H+ antiport across this membrane....
Griffith, Christopher J., Rea, Philip A., Blumwald, Eduardo, Poole, Ronald J.
The H+-ATPase of tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue was studied with respect to the kinetic effects of Cl− and NO3−. N-Ethylmaleimide (NEM) was employed...
Rea, Philip A., Poole, Ronald J.
Membrane vesicles derived from the tonoplast of Beta vulgaris L. possess two predominant phosphohydrolase activities: a Cl−-stimulated, NO3−-inhibited ATPase, and a K+-stimulated, Na+-inhibited...
Martiny-Baron, Georg, Manolson, Morris F., Poole, Ronald J., Hecker, Doris, Scherer, Günther F. E.
The ether phospholipid platelet-activating factor and certain similar phospholipids, including lysophosphatidylcholine, are known to stimulate both H+ transport and protein phosphorylation in plant...
Gerson, Donald F., Poole, Ronald J.
Net uptake of Cl− into root tips of mung bean (Phaseolus aureus) increases steadily with increasing external concentrations from 1 to 60 mm. Membrane potentials were measured to determine the...
Carrier-mediated Potassium Efflux Across the Cell Membrane of Red Beet
The flux ratio (influx/efflux) of K+ across the plasmalemma of beet cells at an external potassium concentration of 0.6 mm does not respond to changes of membrane potential in the manner expected for...
Effect of Sodium on Potassium Fluxes at the Cell Membrane and Vacuole Membrane of Red Beet 1
Slices of red beet (Beta vulgaris) washed for 5 to 6 days are known to accumulate Na+ in preference to K+ from solutions containing both ions. The present work, using ion concentrations of 1.0 mm or...
Development and Characteristics of Sodium-selective Transport in Red Beet 1
Slices of storage tissue of red beet (Beta vulgaris L.) washed for only 1 day in distilled water readily absorb K+ but lack a mechanism for rapid Na+ uptake. A Na+ transport mechanism develops if the...
Blumwald, Eduardo, Poole, Ronald J.
The fluorescent probes acridine orange and oxonol-V were used as indicators of pH gradients (ΔpH) and membrane potential differences (ΔΨ), respectively, in membrane vesicles believed to be derived...
ATP Levels and their Effects on Plasmalemma Influxes of Potassium Chloride in Red Beet 1
Petraglia, Teresa, Poole, Ronald J.
Tissue ATP concentrations in slices of red beet increase progressively with time for up to 7 days after cutting the root. ATP levels are higher in slices taken from stored roots than in slices from...
Effect of Anoxia on ATP Levels and Ion Transport Rates in Red Beet 1
Petraglia, Teresa, Poole, Ronald J.
The ATP content of disks of storage tissue of red beet is reduced under N2 atmosphere to between 10 and 25% of its value in air. Plasmalemma fluxes of K+ and Cl− are inhibited within 1 minute or...
Isolation of Protoplasts and Vacuoles from Storage Tissue of Red Beet 1
Schmidt, Rainer, Poole, Ronald J.
A fast and efficient method is described for the isolation of protoplasts and vacuoles from storage tissue of Beta vulgaris L. The viability of the isolated protoplasts is indicated by the...
Regulation of Cytoplasmic and Vacuolar Volumes by Plant Cells in Suspension Culture 1
Owens, Trevor, Poole, Ronald J.
Quantitative microscopical measurements have been made of the proportion of cell volume occupied by cytoplasm in a cell suspension culture derived from cotyledons of bush bean (cv. Contender). On a...
Inhibition of Na+/H+ Antiport Activity in Sugar Beet Tonoplast by Analogs of Amiloride 1
Blumwald, Eduardo, Cragoe, Edward J., Poole, Ronald J.
The effects of amiloride and a series of amiloride analogs have been tested on the Na+/H+ antiport activity in intact vacuoles and tonoplast vesicles isolated from sugar beet cell suspension...
Alterations in Membrane Protein-Profile during Cold Treatment of Alfalfa 1
Mohapatra, Shyam S., Poole, Ronald J., Dhindsa, Rajinder S.
Changes in pattern of membrane proteins during cold acclimation of alfalfa have been examined. Cold acclimation for 2 to 3 days increases membrane protein content. Labeling of membrane proteins in...
Abscisic Acid-Regulated Gene Expression in Relation to Freezing Tolerance in Alfalfa 1
Mohapatra, Shyam S., Poole, Ronald J., Dhindsa, Rajinder S.
A comparison of abscisic acid (ABA)-induced and cold-acclimation-induced freezing tolerance in two alfalfa cultivars (Medicago falcata cv Anik and Medicago sativa v Trek) indicates that ABA alone can...
Mohapatra, Shyam S., Wolfraim, Lawrence, Poole, Ronald J., Dhindsa, Rajinder S.
Cold-acclimation-specific (CAS) gene expression has been examined by screening a cDNA library prepared from poly(A)+ RNA of cold-acclimated seedlings of a freezing-tolerant variety of alfalfa...
Characterization of Potassium-Dependent Currents in Protoplasts of Corn Suspension Cells 1
Ketchum, Karen A., Shrier, Alvin, Poole, Ronald J.
Protoplasts obtained from corn (Zea mays) suspension cells were studied using the whole cell patch-clamp technique. One time-independent current, as well as two time-dependent currents were...
Blumwald, Eduardo, Poole, Ronald J.
Cell suspension cultures of sugar beet were grown at various salinities (0-200 millimolar NaCl). Their tolerance to Na+ was comparable to that of the intact plant. Tonoplast vesicles were prepared by...
Mohapatra, Shyam S., Poole, Ronald J., Dhindsa, Rajinder S.
Changes in the rate and pattern of protein synthesis and in translatable mRNA population during cold acclimation of alfalfa (Medicago falcata cv Anik) seedlings have been examined. There appears to...
Purification of an H+-Translocating Inorganic Pyrophosphatase from Vacuole Membranes of Red Beet 1
Sarafian, Vahé, Poole, Ronald J.
An H+-translocating inorganic pyrophosphatase (PPase) was isolated and purified from red beet (Beta vulgaris L.) tonoplast. One major polypeptide of molecular weight 67 kilodalton copurified with...
Characterization of the Solubilized Plasma Membrane ATPase of Red Beet 1
Briskin, Donald P., Poole, Ronald J.
The plasma membrane ATP-phosphohydrolase (ATPase) from red beet (Beta vulgaris L.) storage tissue was solubilized with the zwitterionic detergent Zwittergent 3-14 from a plasma membrane-enriched...
Proton-Translocating Inorganic Pyrophosphatase in Red Beet (Beta vulgaris L.) Tonoplast Vesicles 1
Rea, Philip A., Poole, Ronald J.
The substrate and ionic requirements of ATP and inorganic pyrophosphate (PPi) hydrolysis by tonoplast vesicles isolated from storage tissue of red beet (Beta vulgaris L.) were compared with the...
Na+/H+ Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris1
Blumwald, Eduardo, Poole, Ronald J.
The pH-dependent fluorescence quenching of acridine orange was used to study the Na+- and K+-dependent H+ fluxes in tonoplast vesicles isolated from storage tissue of red beet and sugar beet (Beta...
Blumwald, Eduardo, Fortin, Marc G., Rea, Philip A., Verma, Desh Pal S., Poole, Ronald J.
An improved method is described for the isolation of membrane envelope enclosing the bacteroids (peribacteroid membrane) from soybean (Glycine max L.) root nodules. The ATPase activity of the...
Briskin, Donald P., Poole, Ronald J.
A membrane fraction enriched with a magnesium-dependent, monovalent cation-stimulated ATPase was isolated from red beet (Beta vulgaris L.) storage roots by a combination of differential...
Plasma Membrane ATPase of Red Beet Forms a Phosphorylated Intermediate 1
Briskin, Donald P., Poole, Ronald J.
When a plasma membrane-enriched fraction isolated from red beet (Beta vulgaris L.) was incubated in the presence of 40 micromolar [γ-32P] ATP, 40 micromolar MgSO4 at pH 6.5, a rapidly turning over...
Role of Magnesium in the Plasma Membrane ATPase of Red Beet 1
Briskin, Donald P., Poole, Ronald J.
The phosphorylation technique was used to assess the role of Mg in the red beet (Beta vulgaris L.) plasma membrane ATPase. When an excess of ethylenediaminetetraacetate (Tris salt, pH 6.5) was added...
Briskin, Donald P., Poole, Ronald J.
A borohydride reduction method was used to identify the phosphorylated amino acid in the phospho-enzyme of the red beet (Beta vulgaris L.) plasma membrane ATPase. Plasma membrane fractions were...
Poole, Ronald J., Briskin, Donald P., Krátký, Zdeněk, Johnstone, Rose M.
Membranes from homogenates of growing and of dormant storage roots of red beet (Beta vulgaris L.) were centrifuged on linear sucrose gradients. Vanadate-sensitive ATPase activity, a marker for plasma...
Blumwald, Eduardo, Poole, Ronald J.
Artificial pH gradients across tonoplast vesicles isolated from storage tissue of red beet (Beta vulgaris L.) were used to study the kinetics of a Ca2+/H+ antiport across this membrane....
Griffith, Christopher J., Rea, Philip A., Blumwald, Eduardo, Poole, Ronald J.
The H+-ATPase of tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue was studied with respect to the kinetic effects of Cl− and NO3−. N-Ethylmaleimide (NEM) was employed...
Rea, Philip A., Poole, Ronald J.
Membrane vesicles derived from the tonoplast of Beta vulgaris L. possess two predominant phosphohydrolase activities: a Cl−-stimulated, NO3−-inhibited ATPase, and a K+-stimulated, Na+-inhibited...
Martiny-Baron, Georg, Manolson, Morris F., Poole, Ronald J., Hecker, Doris, Scherer, Günther F. E.
The ether phospholipid platelet-activating factor and certain similar phospholipids, including lysophosphatidylcholine, are known to stimulate both H+ transport and protein phosphorylation in plant...
The Influence of the Intracellular Potential on Potassium Uptake by Beetroot Tissue
Intracellular potentials were measured in beetroot tissue during the steady-state uptake of K+ from various solutions. In solutions containing bicarbonate, the membrane potential becomes up to 70 mv...
AUTORADIOGRAPHIC EVIDENCE FOR MANY SEGREGATING DNA MOLECULES IN THE CHLOROPLAST OF OCHROMONAS DANICA
Gibbs, Sarah P., Poole, Ronald J.
Light-grown cells of Ochromonas danica, which contain a single chloroplast per cell, were labeled with [methyl-3H]thymidine for 3 h (0.36 generations) and the distribution of labeled DNA among the...