Martin Meier-Schellersheim, Xuehua Xu, Bastian Angermann, Eric J. Kunkel, Tian Jin, Ronald N. Germain
The signaling network underlying eukaryotic chemosensing is a complex combination of receptor-mediated transmembrane signals, lipid modifications, protein translocations, and differential...
Martin Meier-Schellersheim, Xuehua Xu, Bastian R Angermann, Erik J Kunkel, Tian Jin, Ronald N Germain
The signaling network underlying eukaryotic chemosensing is a complex combination of receptor-mediated transmembrane signals, lipid modifications, protein translocations, and differential...
Modeling T Cell Antigen Discrimination Based on Feedback Control of Digital ERK Responses (2005)
Grégoire Altan-Bonnet, Ronald N. Germain
Computational modeling provide a model which accounts for the high sensitivity, ligand selectivity, and low noise in TCR signaling. Several predictions of the model are supported by experimental data.
Modeling T Cell Antigen Discrimination Based on Feedback Control of Digital ERK Responses (2005)
Grégoire Altan-Bonnet, Ronald N. Germain
T-lymphocyte activation displays a remarkable combination of speed, sensitivity, and discrimination in response to peptide–major histocompatibility complex (pMHC) ligand engagement of clonally...
Huang, Li-Yun, Bikoff, Elizabeth K., Germain, Ronald N., MacDonald, H. R
Cells of Invariant chain-deficient mice show a substantial decrease in cell surface MHC class II protein expression, as well as a change In the occupancy of the expressed class II molecules. Taking...
Day, Patricia M., Yewdell, Jonathan W., Porgador, Angel, Germain, Ronald N., Bennink, Jack R.
After brief incubation of cells with fluorescein-conjugated peptides that bind major histocompatibility complex (MHC) class I molecules, peptides were detected within the endoplasmic reticulum (ER)...
Zhong, Guangming, E Sousa, Caetano Reis, Germain, Ronald N.
Several unanswered questions in T cell immunobiology relating to intracellular processing or in vivo antigen presentation could be approached if convenient, specific, and sensitive reagents were...
Modeling T Cell Antigen Discrimination Based on Feedback Control of Digital ERK Responses
Altan-Bonnet, Grégoire, Germain, Ronald N
T-lymphocyte activation displays a remarkable combination of speed, sensitivity, and discrimination in response to peptide–major histocompatibility complex (pMHC) ligand engagement of clonally...
Itoh, Yasushi, Wang, Ze, Ishida, Hideaki, Eichelberg, Katrin, Fujimoto, Noriki, Makino, Jin, ...
Polarized Th1 and Th2 cells expressing the same TCR produce distinct biochemical responses to ligand engagement. Compared to Th1 cells, Th2 cells show altered substrate tyrosine phosphorylation and a...
An extended vision for dynamic high-resolution intravital immune imaging
Germain, Ronald N., Castellino, Flora, Chieppa, Marcello, Egen, Jackson G., Huang, Alex Y.C., Koo, Lily Y., ...
The past few years have seen the application of confocal and especially two-photon microscopy to the dynamic high-resolution imaging of lymphocytes and antigen presenting cells within organs such as...
Meier-Schellersheim, Martin, Xu, Xuehua, Angermann, Bastian, Kunkel, Eric J, Jin, Tian, Germain, Ronald N
The signaling network underlying eukaryotic chemosensing is a complex combination of receptor-mediated transmembrane signals, lipid modifications, protein translocations, and differential...
Day, Patricia M., Yewdell, Jonathan W., Porgador, Angel, Germain, Ronald N., Bennink, Jack R.
After brief incubation of cells with fluorescein-conjugated peptides that bind major histocompatibility complex (MHC) class I molecules, peptides were detected within the endoplasmic reticulum (ER)...
Zhong, Guangming, E Sousa, Caetano Reis, Germain, Ronald N.
Several unanswered questions in T cell immunobiology relating to intracellular processing or in vivo antigen presentation could be approached if convenient, specific, and sensitive reagents were...
Modeling T Cell Antigen Discrimination Based on Feedback Control of Digital ERK Responses
Altan-Bonnet, Grégoire, Germain, Ronald N
T-lymphocyte activation displays a remarkable combination of speed, sensitivity, and discrimination in response to peptide–major histocompatibility complex (pMHC) ligand engagement of clonally...
Meier-Schellersheim, Martin, Xu, Xuehua, Angermann, Bastian, Kunkel, Eric J, Jin, Tian, Germain, Ronald N
The signaling network underlying eukaryotic chemosensing is a complex combination of receptor-mediated transmembrane signals, lipid modifications, protein translocations, and differential...
Chieppa, Marcello, Rescigno, Maria, Huang, Alex Y.C., Germain, Ronald N.
Cells lining the gastrointestinal tract serve as both a barrier to and a pathway for infectious agent entry. Dendritic cells (DCs) present in the lamina propria under the columnar villus epithelium...
Natural killer cell behavior in lymph nodes revealed by static and real-time imaging
Bajénoff, Marc, Breart, Béatrice, Huang, Alex Y.C., Qi, Hai, Cazareth, Julie, Braud, Veronique M., ...
Natural killer (NK) cells promote dendritic cell (DC) maturation and influence T cell differentiation in vitro. To better understand the nature of the putative interactions among these cells in vivo...
Scheinecker, Clemens, McHugh, Rebecca, Shevach, Ethan M., Germain, Ronald N.
The major histocompatibility complex (MHC)-dependent presentation of processed tissue-specific self-antigens can contribute to either peripheral (extrathymic) tolerance or the differentiation of...
Inaba, Kayo, Turley, Shannon, Iyoda, Tomonori, Yamaide, Fumiya, Shimoyama, Susumu, E Sousa, Caetano Reis, ...
During their final differentiation or maturation, dendritic cells (DCs) redistribute their major histocompatibility complex (MHC) class II products from intracellular compartments to the plasma...
Zhong, Guangming, Romagnoli, Paola, Germain, Ronald N.
Leucine-based signals in the cytoplasmic tail of invariant chain (Ii) control targeting of newly synthesized major histocompatibility complex class II molecules to the endocytic pathway for...
Quantitative Impact of Thymic Clonal Deletion on the T Cell Repertoire
Marguerat, Samuel, Lees, Rosemary K., Germain, Ronald N., Fowlkes, B.J., MacDonald, H. Robson
Interactions between major histocompatibility complex (MHC) molecules expressed on stromal cells and antigen-specific receptors on T cells shape the repertoire of mature T lymphocytes emerging from...
Madrenas, Joaquín, Chau, Luan A., Smith, Judy, Bluestone, Jeffrey A., Germain, Ronald N.
One hypothesis seeking to explain the signaling and biological properties of T cell receptor for antigen (TCR) partial agonists and antagonists is the coreceptor density/kinetic model, which proposes...
Itoh, Yasushi, Germain, Ronald N.
T cell receptor (TCR) recognition of peptide–major histocompatibility complex antigens can elicit a diverse array of effector activities. Here we simultaneously analyze TCR engagement and the...
Zhong, Guangming, Sousa, Caetano Reis E, Germain, Ronald N.
Intravenous (i.v.) injection of high amounts of soluble proteins often results in the induction of antigen-specific tolerance or deviation to helper rather than inflammatory T cell immunity. It has...
Sousa, Caetano Reis E, Hieny, Sara, Scharton-Kersten, Tanya, Jankovic, Dragana, Charest, Hugues, Germain, Ronald N., ...
The early induction of interleukin (IL)-12 is a critical event in determining the development of both innate resistance and adaptive immunity to many intracellular pathogens. Previous in vitro...
Selective Induction of Apoptosis in Mature T Lymphocytes by Variant T Cell Receptor Ligands
Combadière, Behazine, E Sousa, Caetano Reis, Germain, Ronald N., Lenardo, Michael J.
Activation, anergy, and apoptosis are all possible outcomes of T cell receptor (TCR) engagement. The first leads to proliferation and effector function, whereas the others can lead to partial or...
Porgador, Angel, Irvine, Kari R., Iwasaki, Akiko, Barber, Brian H., Restifo, Nicholas P., Germain, Ronald N.
Cutaneous gene (DNA) bombardment results in substantial expression of the encoded antigen in the epidermal layer as well as detectable expression in dendritic cells (DC) in draining lymph nodes...
Castellino, Flora, Boucher, Philip E., Eichelberg, Katrin, Mayhew, Mark, Rothman, James E., Houghton, Alan N., ...
Heat shock proteins (HSPs) derived from tumors or virally infected cells can stimulate antigen-specific CD8+ T cell responses in vitro and in vivo. Although this antigenicity is known to arise from...
In the early 1970s a spate of papers by research groups around the world provided evidence for a negative regulatory role of thymus-derived lymphocytes (T cells). In 1971, Gershon and Kondo published...
Visualization and Identification of IL-7 Producing Cells in Reporter Mice
Mazzucchelli, Renata I., Warming, Søren, Lawrence, Scott M., Ishii, Masaru, Abshari, Mehrnoosh, Washington, A. Valance, ...
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial...