Jean-marc Moncalvo, Dennis Drehmel, Rytas Vilgalys
Modes and rates of molecular evolution, and congruence and combinability for phylogenetic reconstruction, of portions of the nuclear large ribosomal subunit (nLSU-rDNA) and mitochondrial small...
Timothy Y. James, David Porter, Celeste A. Le, Rytas Vilgalys, Joyce E
supports the utility of ultrastructural data in
Moncalvo, Jean-Marc, Lutzoni, François M., Rehner, Stephen A., Johnson, Jacqui, Vilgalys, Rytas
Phylogenetic relationships of mushrooms and their relatives within the order Agaricales were addressed by using nuclear large subunit ribosomal DNA sequences. Approximately 900 bases of the 5′ end...
Clonal and Spontaneous Origins of Fluconazole Resistance in Candida albicans
Xu, Jianping, Ramos, Adela R., Vilgalys, Rytas, Mitchell, Thomas G.
The genotypes and susceptibilities to fluconazole of 78 strains of the human pathogenic yeast Candida albicans were compared. The strains comprised two sets of samples from Durham, N.C.: one from...
Xu, Jianping, Vilgalys, Rytas, Mitchell, Thomas G.
The patterns of genetic variation of samples of Candida albicans isolated from patients infected with human immunodeficiency virus in Durham, N.C., and Vitória, Brazil, were compared. Genotypes for...
Colony Size Can Be Used To Determine the MIC of Fluconazole for Pathogenic Yeasts
Xu, Jianping, Vilgalys, Rytas, Mitchell, Thomas G.
This report describes a new statistical method for estimating the MIC of fluconazole for yeasts pathogenic to humans. This method is based on comparison of the colony sizes on solid media containing...
Litvintseva, Anastasia P., Marra, Robert E., Nielsen, Kirsten, Heitman, Joseph, Vilgalys, Rytas, Mitchell, Thomas G.
The most common cause of fungal meningitis in humans, Cryptococcus neoformans serotype A, is a basidiomycetous yeast with a bipolar mating system. However, the vast majority (>99.9%) of C. neoformans...
Comparative Analysis of Environmental and Clinical Populations of Cryptococcus neoformans
Litvintseva, Anastasia P., Kestenbaum, Lori, Vilgalys, Rytas, Mitchell, Thomas G.
Cryptococcus neoformans is a major, global cause of meningoencephalitis in immunocompromised patients. Despite advances in the molecular epidemiology of C. neoformans, its population structure and...
Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays
Fierer, Noah, Jackson, Jason A., Vilgalys, Rytas, Jackson, Robert B.
Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and...
Fungal Community Analysis by Large-Scale Sequencing of Environmental Samples†
O'Brien, Heath E., Parrent, Jeri Lynn, Jackson, Jason A., Moncalvo, Jean-Marc, Vilgalys, Rytas
Fungi are an important and diverse component of soil communities, but these communities have proven difficult to study in conventional biotic surveys. We evaluated soil fungal diversity at two sites...
James, Timothy Y., Srivilai, Prayook, Kües, Ursula, Vilgalys, Rytas
Mating incompatibility in mushroom fungi is controlled by the mating-type loci. In tetrapolar species, two unlinked mating-type loci exist (A and B), whereas in bipolar species there is only one...
Litvintseva, Anastasia P., Thakur, Rameshwari, Vilgalys, Rytas, Mitchell, Thomas G.
We applied multilocus sequence typing (MLST) to investigate the population structure and mode of reproduction of Cryptococcus neoformans var. grubii (serotype A). This MLST system utilizes 12...
Marra, Robert E, Huang, Johnny C, Fung, Eula, Nielsen, Kirsten, Heitman, Joseph, Vilgalys, Rytas, ...
To construct a genetic linkage map of the heterothallic yeast, Cryptococcus neoformans (Filobasidiella neoformans), we crossed two mating-compatible strains and analyzed 94 progeny for the...
Clonal and Spontaneous Origins of Fluconazole Resistance in Candida albicans
Xu, Jianping, Ramos, Adela R., Vilgalys, Rytas, Mitchell, Thomas G.
The genotypes and susceptibilities to fluconazole of 78 strains of the human pathogenic yeast Candida albicans were compared. The strains comprised two sets of samples from Durham, N.C.: one from...
Xu, Jianping, Vilgalys, Rytas, Mitchell, Thomas G.
The patterns of genetic variation of samples of Candida albicans isolated from patients infected with human immunodeficiency virus in Durham, N.C., and Vitória, Brazil, were compared. Genotypes for...
Colony Size Can Be Used To Determine the MIC of Fluconazole for Pathogenic Yeasts
Xu, Jianping, Vilgalys, Rytas, Mitchell, Thomas G.
This report describes a new statistical method for estimating the MIC of fluconazole for yeasts pathogenic to humans. This method is based on comparison of the colony sizes on solid media containing...
Litvintseva, Anastasia P., Marra, Robert E., Nielsen, Kirsten, Heitman, Joseph, Vilgalys, Rytas, Mitchell, Thomas G.
The most common cause of fungal meningitis in humans, Cryptococcus neoformans serotype A, is a basidiomycetous yeast with a bipolar mating system. However, the vast majority (>99.9%) of C. neoformans...
Comparative Analysis of Environmental and Clinical Populations of Cryptococcus neoformans
Litvintseva, Anastasia P., Kestenbaum, Lori, Vilgalys, Rytas, Mitchell, Thomas G.
Cryptococcus neoformans is a major, global cause of meningoencephalitis in immunocompromised patients. Despite advances in the molecular epidemiology of C. neoformans, its population structure and...
Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays
Fierer, Noah, Jackson, Jason A., Vilgalys, Rytas, Jackson, Robert B.
Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and...
Fungal Community Analysis by Large-Scale Sequencing of Environmental Samples†
O'Brien, Heath E., Parrent, Jeri Lynn, Jackson, Jason A., Moncalvo, Jean-Marc, Vilgalys, Rytas
Fungi are an important and diverse component of soil communities, but these communities have proven difficult to study in conventional biotic surveys. We evaluated soil fungal diversity at two sites...
James, Timothy Y., Srivilai, Prayook, Kües, Ursula, Vilgalys, Rytas
Mating incompatibility in mushroom fungi is controlled by the mating-type loci. In tetrapolar species, two unlinked mating-type loci exist (A and B), whereas in bipolar species there is only one...
Litvintseva, Anastasia P., Thakur, Rameshwari, Vilgalys, Rytas, Mitchell, Thomas G.
We applied multilocus sequence typing (MLST) to investigate the population structure and mode of reproduction of Cryptococcus neoformans var. grubii (serotype A). This MLST system utilizes 12...
Marra, Robert E, Huang, Johnny C, Fung, Eula, Nielsen, Kirsten, Heitman, Joseph, Vilgalys, Rytas, ...
To construct a genetic linkage map of the heterothallic yeast, Cryptococcus neoformans (Filobasidiella neoformans), we crossed two mating-compatible strains and analyzed 94 progeny for the...
Environmental and anthropogenic controls over bacterial communities in wetland soils
Hartman, Wyatt H., Richardson, Curtis J., Vilgalys, Rytas, Bruland, Gregory L.
Soil bacteria regulate wetland biogeochemical processes, yet little is known about controls over their distribution and abundance. Bacteria in North Carolina swamps and bogs differ greatly from...
James, Timothy Y., Litvintseva, Anastasia P., Vilgalys, Rytas, Morgan, Jess A. T., Taylor, John W., Fisher, Matthew C., ...
The fungal disease chytridiomycosis, caused by Batrachochytrium dendrobatidis, is enigmatic because it occurs globally in both declining and apparently healthy (non-declining) amphibian populations....
Findley, Keisha, Rodriguez-Carres, Marianela, Metin, Banu, Kroiss, Johannes, Fonseca, Álvaro, Vilgalys, Rytas, ...
The basidiomycetous yeasts Cryptococcus neoformans and Cryptococcus gattii are closely related sibling species that cause respiratory and neurological disease in humans and animals. Within these two...