Ryuichiro Tanaka

Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

Matsuki, Takahiro, Watanabe, Koichi, Tanaka, Ryuichiro, Fukuda, Masafumi, Oyaizu, Hiroshi

In order to clarify the distribution of bifidobacterial species in the human intestinal tract, a 16S rRNA-gene-targeted species-specific PCR technique was developed and used with DNAs extracted from...

Protective Effect of Lactobacillus casei Strain Shirota on Shiga Toxin-Producing Escherichia coli O157:H7 Infection in Infant Rabbits

Ogawa, Michinaga, Shimizu, Kensuke, Nomoto, Koji, Takahashi, Masatoshi, Watanuki, Masaaki, Tanaka, Ryuichiro, ...

We examined colonization patterns of Shiga toxin-producing Escherichia coli (STEC), concentrations of Shiga toxins (Stxs) and specific immunoglobulin A (lgA) against Stxs and STEC bacterial cell...

Identification, Detection, and Enumeration of Human Bifidobacterium Species by PCR Targeting the Transaldolase Gene

Requena, Teresa, Burton, Jeremy, Matsuki, Takahiro, Munro, Karen, Simon, Mary Alice, Tanaka, Ryuichiro, ...

Methods that enabled the identification, detection, and enumeration of Bifidobacterium species by PCR targeting the transaldolase gene were tested. Bifidobacterial species isolated from the feces of...

Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces

Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Miyamoto, Yukiko, Takada, Toshihiko, Matsumoto, Kazumasa, ...

For the detection and identification of predominant bacteria in human feces, 16S rRNA-gene-targeted group-specific primers for the Bacteroides fragilis group, Bifidobacterium, the Clostridium...

Assessment of Safety of Lactobacillus Strains Based on Resistance to Host Innate Defense Mechanisms

Asahara, Takashi, Takahashi, Masatoshi, Nomoto, Koji, Takayama, Hiroo, Onoue, Masaharu, Morotomi, Masami, ...

Seven Lactobacillus strains belonging to four species were evaluated for pathogenicity as well as for in vitro sensitivity to the bactericidal mechanisms of macrophages in a rabbit infective...

Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria

Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Kado, Yukiko, Takada, Toshihiko, Matsumoto, Kazumasa, ...

A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium...

Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces

Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Takada, Toshihiko, Tanaka, Ryuichiro

16S rRNA gene-targeted group-specific primers were designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster. To monitor the...

The Value of Bile Replacement During External Biliary Drainage: An Analysis of Intestinal Permeability, Integrity, and Microflora

Kamiya, Satoshi, Nagino, Masato, Kanazawa, Hidetoshi, Komatsu, Shunichiro, Mayumi, Toshihiko, Takagi, Kenji, ...

The effect of bile replacement during percutaneous transhepatic biliary drainage was investigated in 25 patients with obstructive jaundice. Intestinal permeability and integrity improved with bile...

Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

Matsuki, Takahiro, Watanabe, Koichi, Tanaka, Ryuichiro, Fukuda, Masafumi, Oyaizu, Hiroshi

In order to clarify the distribution of bifidobacterial species in the human intestinal tract, a 16S rRNA-gene-targeted species-specific PCR technique was developed and used with DNAs extracted from...

Protective Effect of Lactobacillus casei Strain Shirota on Shiga Toxin-Producing Escherichia coli O157:H7 Infection in Infant Rabbits

Ogawa, Michinaga, Shimizu, Kensuke, Nomoto, Koji, Takahashi, Masatoshi, Watanuki, Masaaki, Tanaka, Ryuichiro, ...

We examined colonization patterns of Shiga toxin-producing Escherichia coli (STEC), concentrations of Shiga toxins (Stxs) and specific immunoglobulin A (lgA) against Stxs and STEC bacterial cell...

Identification, Detection, and Enumeration of Human Bifidobacterium Species by PCR Targeting the Transaldolase Gene

Requena, Teresa, Burton, Jeremy, Matsuki, Takahiro, Munro, Karen, Simon, Mary Alice, Tanaka, Ryuichiro, ...

Methods that enabled the identification, detection, and enumeration of Bifidobacterium species by PCR targeting the transaldolase gene were tested. Bifidobacterial species isolated from the feces of...

Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces

Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Miyamoto, Yukiko, Takada, Toshihiko, Matsumoto, Kazumasa, ...

For the detection and identification of predominant bacteria in human feces, 16S rRNA-gene-targeted group-specific primers for the Bacteroides fragilis group, Bifidobacterium, the Clostridium...

Assessment of Safety of Lactobacillus Strains Based on Resistance to Host Innate Defense Mechanisms

Asahara, Takashi, Takahashi, Masatoshi, Nomoto, Koji, Takayama, Hiroo, Onoue, Masaharu, Morotomi, Masami, ...

Seven Lactobacillus strains belonging to four species were evaluated for pathogenicity as well as for in vitro sensitivity to the bactericidal mechanisms of macrophages in a rabbit infective...

Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria

Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Kado, Yukiko, Takada, Toshihiko, Matsumoto, Kazumasa, ...

A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium...

Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces

Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Takada, Toshihiko, Tanaka, Ryuichiro

16S rRNA gene-targeted group-specific primers were designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster. To monitor the...

The Value of Bile Replacement During External Biliary Drainage: An Analysis of Intestinal Permeability, Integrity, and Microflora

Kamiya, Satoshi, Nagino, Masato, Kanazawa, Hidetoshi, Komatsu, Shunichiro, Mayumi, Toshihiko, Takagi, Kenji, ...

The effect of bile replacement during percutaneous transhepatic biliary drainage was investigated in 25 patients with obstructive jaundice. Intestinal permeability and integrity improved with bile...