S. A. Benson

Redundant transfer of F' plasmids occurs between Escherichia coli cells during nonlethal selections.

Peters, J E, Benson, S A

Surface exclusion is the mechanism by which F plasmids prevent the redundant entry of additional F plasmids into the host cell during exponential growth. This mechanism is relaxed in cells that are...

Redundant homosexual F transfer facilitates selection-induced reversion of plasmid mutations.

Peters, J E, Bartoszyk, I M, Dheer, S, Benson, S A

F plasmids use surface exclusion to prevent the redundant entry of additional F plasmids during active growth of the host cells. This mechanism is relaxed during stationary phase and nonlethal...

Isolation and characterization of a generalized transducing phage for Xanthomonas campestris pv. campestris.

Weiss, B D, Capage, M A, Kessel, M, Benson, S A

We have isolated and characterized a lytic double-stranded DNA Xanthomonas campestris pv. campestris bacteriophage (XTP1) capable of mediating generalized transduction. The phage transduces...

Phenotypic expression of the Pseudomonas syringae pv. syringae 61 hrp/hrm gene cluster in Escherichia coli MC4100 requires a functional porin.

Li, T H, Benson, S A, Hutcheson, S W

Plants, in general, appear to be able to detect the presence of incompatible Pseudomonas syringae strains by a hypothetical cell-cell recognition process to initiate inducible defense mechanisms that...

A novel mutation, cog, which results in production of a new porin protein (OmpG) of Escherichia coli K-12.

Misra, R, Benson, S A

A mutant of Escherichia coli K-12 which produces a new outer membrane protein, OmpG, was isolated and genetically and biochemically characterized. The presence of OmpG allows growth on maltodextrins...

Isolation and characterization of OmpC porin mutants with altered pore properties.

Misra, R, Benson, S A

The LamB protein is normally required for the uptake of maltodextrins. Starting with a LamB- OmpF- strain, we have isolated mutants that will grow on maltodextrins. The mutation conferring the Dex+...

Genetic identification of the pore domain of the OmpC porin of Escherichia coli K-12.

Misra, R, Benson, S A

We have isolated and characterized 31 mutations in the ompC gene which allow Escherichia coli to grow on maltotriose (Dex+) in the absence of the LamB and OmpF porins. These ompC(Dex) mutations...

Signal sequence mutations that alter coupling of secretion and translation of an Escherichia coli outer membrane protein.

Benson, S A, Hall, M N, Rasmussen, B A

The lamB701-708 signal sequence mutation reduces expression of LamB, an outer membrane protein of Escherichia coli. To investigate the possibility that synthesis and export of LamB are coupled, as...

Bacteriophage K20 requires both the OmpF porin and lipopolysaccharide for receptor function.

Silverman, J A, Benson, S A

Mutations which prevent absorption of the bacteriophage K20 to Escherichia coli K-12 were selected by using an altered OmpF protein which confers the ability to grow on maltodextrin in the absence of...

Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12.

Benson, S A, Decloux, A

Escherichia coli normally requires the lamB gene for the uptake of maltodextrins. We have identified and characterized three independent mutations that allow E. coli to grow on maltodextrin in the...

Local anesthetics block induction of the Pseudomonas alk regulon.

Benson, S A

The local anesthetics procaine and piperocaine blocked induction of the plasmid-determined enzymatic activities involved in the metabolism of n-alkanes in Pseudomonas putida. Procaine reversibly...

Intragenic regions required for LamB export.

Benson, S A, Bremer, E, Silhavy, T J

Escherichia coli strains containing a series of lamB-lacZ fusions have been isolated and characterized. Each of these fusions specifies a hybrid protein with LamB sequences at the NH2 terminus and a...

Identification and Characterization of a New Gene of Escherichia Coli K-12 Involved in Outer Membrane Permeability

Sampson, B. A., Misra, R., Benson, S. A.

Using a genetic selection for mutations which allow large maltodextrins to cross the outer membrane of Escherichia coli in the absence of the LamB maltoporin, we have obtained and characterized two...

Mutant Bias in Nonlethal Selections Results from Selective Recovery of Mutants

Benson, S. A., DeCloux, A. M., Munro, J.

We have characterized a nonlethal selection for mutations that allow Escherichia coli to grow on large maltodextrins (Dex(+)) in the absence of the lamB encoded maltoporin LamB. These Dex(+)...

Redundant transfer of F' plasmids occurs between Escherichia coli cells during nonlethal selections.

Peters, J E, Benson, S A

Surface exclusion is the mechanism by which F plasmids prevent the redundant entry of additional F plasmids into the host cell during exponential growth. This mechanism is relaxed in cells that are...

Redundant homosexual F transfer facilitates selection-induced reversion of plasmid mutations.

Peters, J E, Bartoszyk, I M, Dheer, S, Benson, S A

F plasmids use surface exclusion to prevent the redundant entry of additional F plasmids during active growth of the host cells. This mechanism is relaxed during stationary phase and nonlethal...

Isolation and characterization of a generalized transducing phage for Xanthomonas campestris pv. campestris.

Weiss, B D, Capage, M A, Kessel, M, Benson, S A

We have isolated and characterized a lytic double-stranded DNA Xanthomonas campestris pv. campestris bacteriophage (XTP1) capable of mediating generalized transduction. The phage transduces...

Phenotypic expression of the Pseudomonas syringae pv. syringae 61 hrp/hrm gene cluster in Escherichia coli MC4100 requires a functional porin.

Li, T H, Benson, S A, Hutcheson, S W

Plants, in general, appear to be able to detect the presence of incompatible Pseudomonas syringae strains by a hypothetical cell-cell recognition process to initiate inducible defense mechanisms that...

A novel mutation, cog, which results in production of a new porin protein (OmpG) of Escherichia coli K-12.

Misra, R, Benson, S A

A mutant of Escherichia coli K-12 which produces a new outer membrane protein, OmpG, was isolated and genetically and biochemically characterized. The presence of OmpG allows growth on maltodextrins...

Isolation and characterization of OmpC porin mutants with altered pore properties.

Misra, R, Benson, S A

The LamB protein is normally required for the uptake of maltodextrins. Starting with a LamB- OmpF- strain, we have isolated mutants that will grow on maltodextrins. The mutation conferring the Dex+...

Genetic identification of the pore domain of the OmpC porin of Escherichia coli K-12.

Misra, R, Benson, S A

We have isolated and characterized 31 mutations in the ompC gene which allow Escherichia coli to grow on maltotriose (Dex+) in the absence of the LamB and OmpF porins. These ompC(Dex) mutations...

Signal sequence mutations that alter coupling of secretion and translation of an Escherichia coli outer membrane protein.

Benson, S A, Hall, M N, Rasmussen, B A

The lamB701-708 signal sequence mutation reduces expression of LamB, an outer membrane protein of Escherichia coli. To investigate the possibility that synthesis and export of LamB are coupled, as...

Bacteriophage K20 requires both the OmpF porin and lipopolysaccharide for receptor function.

Silverman, J A, Benson, S A

Mutations which prevent absorption of the bacteriophage K20 to Escherichia coli K-12 were selected by using an altered OmpF protein which confers the ability to grow on maltodextrin in the absence of...

Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12.

Benson, S A, Decloux, A

Escherichia coli normally requires the lamB gene for the uptake of maltodextrins. We have identified and characterized three independent mutations that allow E. coli to grow on maltodextrin in the...

Local anesthetics block induction of the Pseudomonas alk regulon.

Benson, S A

The local anesthetics procaine and piperocaine blocked induction of the plasmid-determined enzymatic activities involved in the metabolism of n-alkanes in Pseudomonas putida. Procaine reversibly...

Intragenic regions required for LamB export.

Benson, S A, Bremer, E, Silhavy, T J

Escherichia coli strains containing a series of lamB-lacZ fusions have been isolated and characterized. Each of these fusions specifies a hybrid protein with LamB sequences at the NH2 terminus and a...

Identification and Characterization of a New Gene of Escherichia Coli K-12 Involved in Outer Membrane Permeability

Sampson, B. A., Misra, R., Benson, S. A.

Using a genetic selection for mutations which allow large maltodextrins to cross the outer membrane of Escherichia coli in the absence of the LamB maltoporin, we have obtained and characterized two...

Mutant Bias in Nonlethal Selections Results from Selective Recovery of Mutants

Benson, S. A., DeCloux, A. M., Munro, J.

We have characterized a nonlethal selection for mutations that allow Escherichia coli to grow on large maltodextrins (Dex(+)) in the absence of the lamB encoded maltoporin LamB. These Dex(+)...