Gourse, R L, Thurlow, D L, Gerbi, S A, Zimmermann, R A
Ribosomal protein L1 from the prokaryote Escherichia coli has been shown to form a specific complex with 26S ribosomal RNA from the eukaryote Dictyostelium discoideum. The segment of Dictyostelium...
Clark, C G, Tague, B W, Ware, V C, Gerbi, S A
Based upon the three experimentally derived models of E. coli 23S rRNA (1-3) and the partial model for yeast 26S rRNA (4), which was deduced by homology to E. coli, we derived a secondary structure...
Sequence analysis of bovine satellite I DNA (1.715 gm/cm3).
The 1402 bp Eco RI repeating unit of bovine satellite I DNA (rho CsCl = 1.715 gm/cm3) has been cloned in pBR322. The sequence of this cloned repeat has been determined and is greater than 97%...
The basic repeat unit of a Chironomus Balbiani ring gene.
Bäumlein, H, Wobus, U, Gerbi, S A, Kafatos, F C
A clone derived from the Balbiani ring b (BRb) gene of Chironomus thummi has been used to study the internal organization of that gene. Much of the gene consists of approximately 80 copies of a ca....
Structure of 1.71 lb gm/cm(3) bovine satellite DNA: evolutionary relationship to satellite I.
The Eco RI fragments from the 2600 bp repeating unit of 1.711b gm/Cm(3) bovine satellite DNA were cloned in pBR322. The structure of the repeat unit was determined and compared to bovine satellite I...
By hybridization and DNA sequencing, we have defined a specific region in Xenopus rDNA that is extremely conserved between Tetrahymena, a protozoan, and Xenopus, a vertebrate. This highly conserved...
Sequence analysis of 28S ribosomal DNA from the amphibian Xenopus laevis.
Ware, V C, Tague, B W, Clark, C G, Gourse, R L, Brand, R C, Gerbi, S A
We have determined the complete nucleotide sequence of Xenopus laevis 28S rDNA (4110 bp). In order to locate evolutionarily conserved regions within rDNA, we compared the Xenopus 28S sequence to...
Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.
Renkawitz-Pohl, R, Matsumoto, L, Gerbi, S A
We have prepared a partial gene library of sheared DNA from the fungus fly, Sciara coprophila, by dA-T tailing and insertion into pBR322. Two ribosomal DNA clones which differ from the usual...
Genes for Xenopus laevis U3 small nuclear RNA.
Savino, R, Hitti, Y, Gerbi, S A
Genomic Southern blots showed there are only 14 to 20 copies of U3 snRNA genes per somatic genome in Xenopus laevis, unlike the highly repetitive, tandem arrangement of other snRNA genes in this...
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
Jeppesen, C, Stebbins-Boaz, B, Gerbi, S A
Using a combination of RNA sequencing and construction of cDNA clones followed by DNA sequencing, we have determined the primary nucleotide sequence of U3 snRNA in Xenopus laevis and Xenopus...
Ware, V C, Renkawitz, R, Gerbi, S A
We have determined the sequence of the rDNA region between the 28S alpha and 28S beta rRNA coding segments (termed a "gap") in the insect Sciara coprophila, and have used S1 nuclease mapping and cDNA...
The distribution of methyl groups in rRNA from Xenopus laevis was analyzed by hybridization of rRNA to subfragments of either of two cloned rDNA fragments, X1r11 and X1r12, which together constitute...
The replication origin region for DNA amplification in Sciara coprophila DNA puff II/9A was analyzed with a novel three-dimensional (3D) gel method. Our 3D gel method involves running a...
Bienz-Tadmor, B, Smith, H S, Gerbi, S A
DNA puffs occur in Sciarid salivary gland chromosomes; they are sites of DNA amplification and intense transcription and they appear to encode secreted structural proteins needed for pupation. In...
Changes in 7SL RNA conformation during the signal recognition particle cycle.
The structure of 7SL RNA has been probed by chemical modification followed by primer extension, using four substrates: (i) naked 7SL RNA; (ii) free signal recognition particle (SRP); (iii) polysome...
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
DNA oligonucleotide complementary to sequences in the 5' third of U3 snRNA were injected into Xenopus oocyte nuclei to disrupt endogenous U3 snRNA. The effect of this treatment on rRNA processing was...
Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.
Lange, T S, Borovjagin, A, Maxwell, E S, Gerbi, S A
Sequences necessary for nucleolar targeting were identified in Box C/D small nucleolar RNAs (snoRNAs) by fluorescence microscopy. Nucleolar preparations were examined after injecting...
Nucleolar localization elements in U8 snoRNA differ from sequences required for rRNA processing.
Lange, T S, Borovjagin, A V, Gerbi, S A
U8 small nucleolar RNA (snoRNA) is essential for metazoan ribosomal RNA (rRNA) processing in nucleoli. The sequences and structural features in Xenopus U8 snoRNA that are required for its nucleolar...
Gourse, R L, Thurlow, D L, Gerbi, S A, Zimmermann, R A
Ribosomal protein L1 from the prokaryote Escherichia coli has been shown to form a specific complex with 26S ribosomal RNA from the eukaryote Dictyostelium discoideum. The segment of Dictyostelium...
Clark, C G, Tague, B W, Ware, V C, Gerbi, S A
Based upon the three experimentally derived models of E. coli 23S rRNA (1-3) and the partial model for yeast 26S rRNA (4), which was deduced by homology to E. coli, we derived a secondary structure...
Sequence analysis of bovine satellite I DNA (1.715 gm/cm3).
The 1402 bp Eco RI repeating unit of bovine satellite I DNA (rho CsCl = 1.715 gm/cm3) has been cloned in pBR322. The sequence of this cloned repeat has been determined and is greater than 97%...
The basic repeat unit of a Chironomus Balbiani ring gene.
Bäumlein, H, Wobus, U, Gerbi, S A, Kafatos, F C
A clone derived from the Balbiani ring b (BRb) gene of Chironomus thummi has been used to study the internal organization of that gene. Much of the gene consists of approximately 80 copies of a ca....
Structure of 1.71 lb gm/cm(3) bovine satellite DNA: evolutionary relationship to satellite I.
The Eco RI fragments from the 2600 bp repeating unit of 1.711b gm/Cm(3) bovine satellite DNA were cloned in pBR322. The structure of the repeat unit was determined and compared to bovine satellite I...
By hybridization and DNA sequencing, we have defined a specific region in Xenopus rDNA that is extremely conserved between Tetrahymena, a protozoan, and Xenopus, a vertebrate. This highly conserved...
Sequence analysis of 28S ribosomal DNA from the amphibian Xenopus laevis.
Ware, V C, Tague, B W, Clark, C G, Gourse, R L, Brand, R C, Gerbi, S A
We have determined the complete nucleotide sequence of Xenopus laevis 28S rDNA (4110 bp). In order to locate evolutionarily conserved regions within rDNA, we compared the Xenopus 28S sequence to...
Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.
Renkawitz-Pohl, R, Matsumoto, L, Gerbi, S A
We have prepared a partial gene library of sheared DNA from the fungus fly, Sciara coprophila, by dA-T tailing and insertion into pBR322. Two ribosomal DNA clones which differ from the usual...
Genes for Xenopus laevis U3 small nuclear RNA.
Savino, R, Hitti, Y, Gerbi, S A
Genomic Southern blots showed there are only 14 to 20 copies of U3 snRNA genes per somatic genome in Xenopus laevis, unlike the highly repetitive, tandem arrangement of other snRNA genes in this...
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
Jeppesen, C, Stebbins-Boaz, B, Gerbi, S A
Using a combination of RNA sequencing and construction of cDNA clones followed by DNA sequencing, we have determined the primary nucleotide sequence of U3 snRNA in Xenopus laevis and Xenopus...
Ware, V C, Renkawitz, R, Gerbi, S A
We have determined the sequence of the rDNA region between the 28S alpha and 28S beta rRNA coding segments (termed a "gap") in the insect Sciara coprophila, and have used S1 nuclease mapping and cDNA...
The distribution of methyl groups in rRNA from Xenopus laevis was analyzed by hybridization of rRNA to subfragments of either of two cloned rDNA fragments, X1r11 and X1r12, which together constitute...
The replication origin region for DNA amplification in Sciara coprophila DNA puff II/9A was analyzed with a novel three-dimensional (3D) gel method. Our 3D gel method involves running a...
Bienz-Tadmor, B, Smith, H S, Gerbi, S A
DNA puffs occur in Sciarid salivary gland chromosomes; they are sites of DNA amplification and intense transcription and they appear to encode secreted structural proteins needed for pupation. In...
Changes in 7SL RNA conformation during the signal recognition particle cycle.
The structure of 7SL RNA has been probed by chemical modification followed by primer extension, using four substrates: (i) naked 7SL RNA; (ii) free signal recognition particle (SRP); (iii) polysome...
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
DNA oligonucleotide complementary to sequences in the 5' third of U3 snRNA were injected into Xenopus oocyte nuclei to disrupt endogenous U3 snRNA. The effect of this treatment on rRNA processing was...
Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.
Lange, T S, Borovjagin, A, Maxwell, E S, Gerbi, S A
Sequences necessary for nucleolar targeting were identified in Box C/D small nucleolar RNAs (snoRNAs) by fluorescence microscopy. Nucleolar preparations were examined after injecting...
Nucleolar localization elements in U8 snoRNA differ from sequences required for rRNA processing.
Lange, T S, Borovjagin, A V, Gerbi, S A
U8 small nucleolar RNA (snoRNA) is essential for metazoan ribosomal RNA (rRNA) processing in nucleoli. The sequences and structural features in Xenopus U8 snoRNA that are required for its nucleolar...