Thermal force modeling for precise prediction and determination of spacecraft orbits (2005)
Adhya, S., Ziebart, M., Sibthorpe, A., Arrowsmith, P., Cross, P.
The physical modeling of nonconservative forces is a limiting factor in orbit prediction. One such force arises from the anisotropic emission of thermal radiation from a satellite's surface, which...
Ziebart, M., Adhya, S., Sibthorpe, A., Edwards, S., Cross, P.
In an era of high resolution gravity field modelling the dominant error sources in spacecraft orbit determination are non-conservative spacecraft surface forces. These forces can be difficult to...
Toxoplasmosis in Darjeeling. (1996)
Adhya, S, Chakraborty, P, Chakraborty, M, Bhattacharya, S, Pal, B, Sati, A, ...
Jakubczak, J L, Merlino, G, French, J E, Muller, W J, Paul, B, Adhya, S, ...
Genetic instability is thought to be responsible for the numerous genotypic changes that occur during neoplastic transformation and metastatic progression. To explore the role of genetic instability...
Long-circulating bacteriophage as antibacterial agents.
Merril, C R, Biswas, B, Carlton, R, Jensen, N C, Creed, G J, Zullo, S, ...
The increased prevalence of multidrug-resistant bacterial pathogens motivated us to attempt to enhance the therapeutic efficacy of bacteriophages. The therapeutic application of phages as...
Transcription regulation by inflexibility of promoter DNA in a looped complex.
Choy, H E, Park, S W, Parrack, P, Adhya, S
The gal operon of Escherichia coli is negatively regulated by repressor binding to bipartite operators separated by 11 helical turns of DNA. Synergistic binding of repressor to separate sites on DNA...
An arcane role of DNA in transcription activation.
The mechanism by which the cAMP receptor protein (CRP) activates transcription has been investigated using the lac promoter of Escherichia coli. For transcription activation, an interaction between...
Ryu, S, Kim, J, Adhya, S, Garges, S
The cAMP receptor protein (CRP) of Escherichia coli needs cAMP for an allosteric change to regulate gene expression by binding to specific DNA sites. The hinge region connecting the DNA-binding...
We have developed an in vivo system to engender supercoiled "minicircle" DNA containing a single promoter by using the integrative recombination system of bacteriophage lambda. The resulting...
Fox, D K, Presper, K A, Adhya, S, Roseman, S, Garges, S
Several potential target sites for multiple regulatory mechanisms were previously identified in the 5' flanking region of the pts operon. We have investigated the in vitro interactions of the cAMP...
Allosteric changes in the cAMP receptor protein of Escherichia coli: hinge reorientation.
The cAMP receptor protein (CRP) of Escherichia coli is a dimer of a two-domain subunit. It requires binding of cAMP for a conformational change in order to function as a site-specific DNA-binding...
Control of gal transcription through DNA looping: inhibition of the initial transcribing complex.
Involvement of DNA looping between two spatially separated gal operators, OE and OI, in repression of the gal operon has been demonstrated in vivo. An in vitro transcription assay using a minicircle...
Atomic force microscopic demonstration of DNA looping by GalR and HU.
Lyubchenko, Y L, Shlyakhtenko, L S, Aki, T, Adhya, S
Regulation of gene transcription in both prokaryotes and eukaryotes involves formation of various DNA-multiprotein complexes of higher order structure through communication between distant regions of...
The D arm of tRNATyr is necessary and sufficient for import into Leishmania mitochondria in vitro.
Mahapatra, S, Ghosh, S, Bera, S K, Ghosh, T, Das, A, Adhya, S
Transfer RNAs are selectively imported from the cytoplasm into mitochondria of kinetoplastid protozoa such as Leishmania . The specific structural features of tRNA which determine selectivity are...
Functional characterization of roles of GalR and GalS as regulators of the gal regulon.
An isorepressor of the gal regulon in Escherichia coli, GalS, has been purified to homogeneity. In vitro DNase I protection experiments indicated that among operators of the gal regulon, GalS binds...
Control of transcription of gal repressor and isorepressor genes in Escherichia coli.
Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the...
Further inducibility of a constitutive system: ultrainduction of the gal operon.
Tokeson, J P, Garges, S, Adhya, S
In wild-type Escherichia coli, expression of the gal operon is negatively regulated by the Gal repressor and is induced 10- to 15-fold when the repressor is inactivated by an inducer. In strains...
A mutation defining ultrainduction of the Escherichia coli gal operon.
Golding, A, Weickert, M J, Tokeson, J P, Garges, S, Adhya, S
Tn10 insertion in the galS (ultrainduction factor) gene of Escherichia coli allows the gal operon to be constitutively expressed at a very high level, equal to that seen in a delta galR strain in the...
We have studied the initiation of transcription of the gal operon in Escherichia coli (i) by analyzing the 5'-triphosphate ends and (ii) by measuring the level of promoter-proximal gal mRNA made in...
We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced...
Guanylate cyclase activity in Escherichia coli mutants defective in adenylate cyclase.
Macchia, V, Caputo, G, Mandato, E, Rocino, A, Adhya, S, Pastan, I
Guanylate cyclase, which catalyzes the synthesis of guanosine 3',5'-monophosphate, has been assayed in several strains of Escherichia coli. They include wild-type cells and mutants defective in...
Reyes, O, Gottesman, M, Adhya, S
Bacterial mutations (psuA and psu) known for their ability to suppress the polarity on nonsense mutations are shown to suppress the polarity of certain insertion mutations in the gal operon. The...
Lorberboum-Galski, H, FitzGerald, D, Chaudhary, V, Adhya, S, Pastan, I
A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric...
Chaudhary, V K, Xu, Y H, FitzGerald, D, Adhya, S, Pastan, I
Pseudomonas exotoxin (PE) is composed of structural domains I, II, and III; when interacting with mammalian cells the function of domain I is cell recognition, the function of domain II is membrane...
Ren, Y L, Garges, S, Adhya, S, Krakow, J S
Four cAMP-independent receptor protein mutants (designated CRP* mutants) isolated previously are able to activate in vivo gene transcription in the absence of cAMP and their activity can be enhanced...
Two operators, spatially separated from each other and from the promoters, repress the gal operon when bound to Gal repressor. Conversion of either gal operator to a lac operator results in...
Chaudhary, V K, FitzGerald, D J, Adhya, S, Pastan, I
The transforming growth factor type alpha gene has been fused to a modified Pseudomonas toxin gene from which the cell-recognition domain has been deleted. The chimeric gene has been expressed in...
Interaction of small ribosomal and transfer RNAs with a protein from Leishmania donovani.
Ghosh, A, Ghosh, T, Ghosh, S, Das, S, Adhya, S
Using synthetic antisense RNA from the 5'-untranslated region of the beta-tubulin gene as probe in gel retardation assays, a heat stable RNA-binding factor was identified in promastigotes of the...
Location, function, and nucleotide sequence of a promoter for bacteriophage T3 RNA polymerase.
Adhya, S, Basu, S, Sarkar, P, Maitra, U
The major promoters for bacteriophage T3 RNA polymerase on the T3 genome have been mapped by DNA.RNA filter hybridization. One promoter is located in a 300-base-pair Hpa I restriction fragment near...
Ren, Y L, Garges, S, Adhya, S, Krakow, J S
Wild type cAMP receptor protein (CRP) activates in vitro lac transcription only in the presence of cAMP. In contrast the mutant CRP*598 (Arg-142 to His, Ala-144 to Thr) can activate lac transcription...
Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.
We have previously described temperature sensitive rho mutants of Escherichia coli (e.g., rho15) that are defective in transcription termination at various signals, including an IS2 DNA insertion in...
Gal repressor dimer binds to two gal operator sites, OE and OI, which are 16 bp long similar sequences with hyphenated dyad symmetries (11,12). Repressor occupation hinders the reactivity of the N7...
Greenblatt, J, Li, J, Adhya, S, Friedman, D I, Baron, L S, Redfield, B, ...
The DNA-dependent in vitro synthesis of Escherichia coli beta-galactosidase requires the presence of a soluble protein referred to as L factor [Kung, H., Spears, C. & Weissbach, H. (1975) J. Biol....
Site-specific DNA binding of nuclear factor I: analyses of cellular binding sites.
Gronostajski, R M, Adhya, S, Nagata, K, Guggenheimer, R A, Hurwitz, J
Nuclear factor I is a cellular site-specific DNA-binding protein required for the efficient in vitro replication of adenovirus DNA. We have characterized human DNA sequences to which nuclear factor I...
Irani, M, Orosz, L, Busby, S, Taniguchi, T, Adhya, S
When the gal operator region is present in a multicopy plasmid it binds to all ("titrates") the gal repressor and "induces" the chromosomal gal operon. To make operator mutations (Oa) with reduced...
Demonstration of two operator elements in gal: in vitro repressor binding studies.
Genetic and DNA base sequence analyses of cis-dominant mutations that derepress the gal operon of Escherichia coli suggested the existence of two operator loci needed for gal repression. One (OE) is...
Choy, H E, Park, S W, Aki, T, Parrack, P, Fujita, N, Ishihama, A, ...
Gal or Lac repressor binding to an upstream DNA segment, in the absence of DNA looping, represses the P1 promoter located on the same face and activates the P2 promoter situated on the opposite face...
Polarity suppressor mutants that are conditional lethal for growth have been isolated in E. coli K12. The mutations map between the ilv and cya loci of the E. coli chromosome. Rho factor isolated...
Import of small RNAs into Leishmania mitochondria in vitro.
Mahapatra, S, Ghosh, T, Adhya, S
Using an in vitro ribonuclease protection assay, it was shown that synthetic antisense transcripts from the 5'-upstream region of the beta-tubulin gene are efficiently imported into isolated...
Repressor induced site-specific binding of HU for transcriptional regulation.
Transcription from two overlapping gal promoters is repressed by Gal repressor binding to bipartite gal operators, O(E) and O(I), which flank the promoters. Concurrent repression of the gal promoters...
Chakraborty, R, Dasgupta, D, Adhya, S, Basu, M K
Antisense oligonucleotides have been considered as inhibitors of growth of intracellular parasites such as Leishmania, but only limited inhibition has been observed in vitro. We have encapsulated an...
Jakubczak, J L, Merlino, G, French, J E, Muller, W J, Paul, B, Adhya, S, ...
Genetic instability is thought to be responsible for the numerous genotypic changes that occur during neoplastic transformation and metastatic progression. To explore the role of genetic instability...
Long-circulating bacteriophage as antibacterial agents.
Merril, C R, Biswas, B, Carlton, R, Jensen, N C, Creed, G J, Zullo, S, ...
The increased prevalence of multidrug-resistant bacterial pathogens motivated us to attempt to enhance the therapeutic efficacy of bacteriophages. The therapeutic application of phages as...
Transcription regulation by inflexibility of promoter DNA in a looped complex.
Choy, H E, Park, S W, Parrack, P, Adhya, S
The gal operon of Escherichia coli is negatively regulated by repressor binding to bipartite operators separated by 11 helical turns of DNA. Synergistic binding of repressor to separate sites on DNA...
An arcane role of DNA in transcription activation.
The mechanism by which the cAMP receptor protein (CRP) activates transcription has been investigated using the lac promoter of Escherichia coli. For transcription activation, an interaction between...
Ryu, S, Kim, J, Adhya, S, Garges, S
The cAMP receptor protein (CRP) of Escherichia coli needs cAMP for an allosteric change to regulate gene expression by binding to specific DNA sites. The hinge region connecting the DNA-binding...
We have developed an in vivo system to engender supercoiled "minicircle" DNA containing a single promoter by using the integrative recombination system of bacteriophage lambda. The resulting...
Fox, D K, Presper, K A, Adhya, S, Roseman, S, Garges, S
Several potential target sites for multiple regulatory mechanisms were previously identified in the 5' flanking region of the pts operon. We have investigated the in vitro interactions of the cAMP...
Allosteric changes in the cAMP receptor protein of Escherichia coli: hinge reorientation.
The cAMP receptor protein (CRP) of Escherichia coli is a dimer of a two-domain subunit. It requires binding of cAMP for a conformational change in order to function as a site-specific DNA-binding...
Control of gal transcription through DNA looping: inhibition of the initial transcribing complex.
Involvement of DNA looping between two spatially separated gal operators, OE and OI, in repression of the gal operon has been demonstrated in vivo. An in vitro transcription assay using a minicircle...
Atomic force microscopic demonstration of DNA looping by GalR and HU.
Lyubchenko, Y L, Shlyakhtenko, L S, Aki, T, Adhya, S
Regulation of gene transcription in both prokaryotes and eukaryotes involves formation of various DNA-multiprotein complexes of higher order structure through communication between distant regions of...
The D arm of tRNATyr is necessary and sufficient for import into Leishmania mitochondria in vitro.
Mahapatra, S, Ghosh, S, Bera, S K, Ghosh, T, Das, A, Adhya, S
Transfer RNAs are selectively imported from the cytoplasm into mitochondria of kinetoplastid protozoa such as Leishmania . The specific structural features of tRNA which determine selectivity are...
Functional characterization of roles of GalR and GalS as regulators of the gal regulon.
An isorepressor of the gal regulon in Escherichia coli, GalS, has been purified to homogeneity. In vitro DNase I protection experiments indicated that among operators of the gal regulon, GalS binds...
Control of transcription of gal repressor and isorepressor genes in Escherichia coli.
Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the...
Further inducibility of a constitutive system: ultrainduction of the gal operon.
Tokeson, J P, Garges, S, Adhya, S
In wild-type Escherichia coli, expression of the gal operon is negatively regulated by the Gal repressor and is induced 10- to 15-fold when the repressor is inactivated by an inducer. In strains...
A mutation defining ultrainduction of the Escherichia coli gal operon.
Golding, A, Weickert, M J, Tokeson, J P, Garges, S, Adhya, S
Tn10 insertion in the galS (ultrainduction factor) gene of Escherichia coli allows the gal operon to be constitutively expressed at a very high level, equal to that seen in a delta galR strain in the...
We have studied the initiation of transcription of the gal operon in Escherichia coli (i) by analyzing the 5'-triphosphate ends and (ii) by measuring the level of promoter-proximal gal mRNA made in...
We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced...
Guanylate cyclase activity in Escherichia coli mutants defective in adenylate cyclase.
Macchia, V, Caputo, G, Mandato, E, Rocino, A, Adhya, S, Pastan, I
Guanylate cyclase, which catalyzes the synthesis of guanosine 3',5'-monophosphate, has been assayed in several strains of Escherichia coli. They include wild-type cells and mutants defective in...
Reyes, O, Gottesman, M, Adhya, S
Bacterial mutations (psuA and psu) known for their ability to suppress the polarity on nonsense mutations are shown to suppress the polarity of certain insertion mutations in the gal operon. The...
Lorberboum-Galski, H, FitzGerald, D, Chaudhary, V, Adhya, S, Pastan, I
A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric...
Chaudhary, V K, Xu, Y H, FitzGerald, D, Adhya, S, Pastan, I
Pseudomonas exotoxin (PE) is composed of structural domains I, II, and III; when interacting with mammalian cells the function of domain I is cell recognition, the function of domain II is membrane...
Ren, Y L, Garges, S, Adhya, S, Krakow, J S
Four cAMP-independent receptor protein mutants (designated CRP* mutants) isolated previously are able to activate in vivo gene transcription in the absence of cAMP and their activity can be enhanced...
Two operators, spatially separated from each other and from the promoters, repress the gal operon when bound to Gal repressor. Conversion of either gal operator to a lac operator results in...
Chaudhary, V K, FitzGerald, D J, Adhya, S, Pastan, I
The transforming growth factor type alpha gene has been fused to a modified Pseudomonas toxin gene from which the cell-recognition domain has been deleted. The chimeric gene has been expressed in...