Immunobiology of human imunodeficiency virus infection (2007)
After the discovery of human immunodeficiency virus (HIV) and its role in the causation of most devastating epidemic acquired immune deficiency syndrome (AIDS), there has been an increasing trend to...
Single-centre experience of laparoscopic nephrectomy: Impact of learning curve on outcome (2007)
Jha, MS, Gupta, N, Agrawal, S, Ansari, M, Dubey, D, Mandhani, A, ...
Nephron sparing surgery: A single institution experience (2007)
Agrawal, S, Jha, M, Khurana, N, Ansari, M, Dubey, D, Srivastava, A, ...
Khurana, N, Lavania, P, Goyal, R, Agrawal, S, Dubey, D, Mandhani, A, ...
Victor, P., Kumar, A., Lupo, F., Gandhi, D., Agrawal, S., Ramanath, G., ...
Victor, P., Kumar, A., Lupo, F., Gandhi, D., Agrawal, S., Ramanath, G., ...
TNFR2 gene polymorphism in coronary artery disease (2005)
Sankar, V, Girisha, K, Gilmour, A, Singh, V, Sinha, N, Tewari, S, ...
Girisha, K, Gilmour, A, Mastana, S, Singh, V, Sinha, N, Tewari, S, ...
Optimizing last dissolving Dosage form of Diclofenac Sodium by rapidly disintegrating agents. (2003)
Harmonisation, mosaicing and production of the Global Land Cover 2000 database (beta version) (2003)
Fritz, S., Bartholomé, E., Belward, A., Hartley, A., Stibig, H.J., Eva, H., ...
Milicic, A, Misra, R, Agrawal, S, Aggarwal, A, Brown, MA, Wordsworth, BP
Objectives: To investigate the association of the FcgammaRIIIA gene with rheumatoid arthritis (RA) in two genetically distinct groups: a white group from the United Kingdom and a northern Indian...
Congenital hernia through the foramen of Morgagni (a case report). (1990)
Sortey, D, Mehta, M, Jain, P, Agrawal, S, Gadkari, S, Raghavan, V, ...
Lisziewicz, J, Sun, D, Weichold, F F, Thierry, A R, Lusso, P, Tang, J, ...
Gene-expression modulator 91 (GEM91) is a 25-nt antisense oligodeoxynucleotide phosphorothioate complementary to the Gag mRNA of human immunodeficiency virus type 1 (HIV-1). Cellular uptake and...
Lisziewicz, J, Sun, D, Metelev, V, Zamecnik, P, Gallo, R C, Agrawal, S
The antiviral activity of antisense oligodeoxynucleotide phosphorothioates complementary to the tat gene, the gag mRNA, and the rev mRNA were studied in a long-term infection model. Three antisense...
Rapaport, E, Misiura, K, Agrawal, S, Zamecnik, P
Synthetic oligonucleotides and their chemical modifications have been shown to inhibit viral and cellular gene expression by sequence-specific antisense hybridization to target mRNAs. We now report...
Lisziewicz, J, Sun, D, Klotman, M, Agrawal, S, Zamecnik, P, Gallo, R
We have developed a culture system, simulating in vivo conditions of human immunodeficiency virus type 1 (HIV-1) infection, to evaluate the long-term efficacy of antisense oligonucleotide treatment....
Pharmacokinetics, biodistribution, and stability of oligodeoxynucleotide phosphorothioates in mice.
Agrawal, S, Temsamani, J, Tang, J Y
We describe preliminary studies of the pharmacokinetics, biodistribution, and excretion of an oligodeoxy-nucleotide phosphorothioate ([S]oligonucleotide) in mice. After either intravenous or...
Site-specific excision from RNA by RNase H and mixed-phosphate-backbone oligodeoxynucleotides.
Agrawal, S, Mayrand, S H, Zamecnik, P C, Pederson, T
Oligodeoxynucleotides containing phosphodiester or modified internucleoside linkages were investigated with respect to their ability to be acted on by ribonuclease H activities present in a HeLa cell...
Inhibition of influenza virus replication by phosphorothioate oligodeoxynucleotides.
Leiter, J M, Agrawal, S, Palese, P, Zamecnik, P C
Oligodeoxynucleotides (ODNs) were synthesized and tested for their antiviral activity against influenza viruses. ODNs corresponded to the polymerase PB1 gene of either influenza A/WSN/33 virus or...
The In Vitro-Synthesized RNA from a cDNA Clone of Hepatitis E Virus Is Infectious
Panda, S. K., Ansari, I. H., Durgapal, H., Agrawal, S., Jameel, S.
Hepatitis E virus (HEV) is an important etiological agent of epidemic and sporadic hepatitis, which is endemic to the Indian subcontinent and prevalent in most of the developing parts of the world....
Kandimalla, E R, Manning, A, Zhao, Q, Shaw, D R, Byrn, R A, Sasisekharan, V, ...
We have designed and synthesized mixed backbone oligonucleotides (MBOs) containing 2'-5'-ribo- and 3'-5'-deoxyribonucleotide segments. Thermal melting studies of the phosphodiester MBOs (three...
Veal, G J, Agrawal, S, Byrn, R A
We have used a ribonuclease protection assay to investigate RNase H cleavage of HIV-1 mRNA mediated by phosphorothioate antisense oligonucleotides complementary to the gag region of the HIV-1 genome...
Inhibition of human immunodeficiency virus replication by antisense oligodeoxynucleotides.
Goodchild, J, Agrawal, S, Civeira, M P, Sarin, P S, Sun, D, Zamecnik, P C
Twenty different target sites within human immunodeficiency virus (HIV) RNA were selected for studies of inhibition of HIV replication by antisense oligonucleotides. Target sites were selected based...
Agrawal, S, Goodchild, J, Civeira, M P, Thornton, A H, Sarin, P S, Zamecnik, P C
Modified oligodeoxynucleotides complementary to RNA of human immunodeficiency virus 1 (HIV-1) were tested for their ability to inhibit virally induced syncytium formation and expression of viral p24...
Inhibition of acquired immunodeficiency syndrome virus by oligodeoxynucleoside methylphosphonates.
Sarin, P S, Agrawal, S, Civeira, M P, Goodchild, J, Ikeuchi, T, Zamecnik, P C
Antisense oligodeoxynucleotides containing internucleoside methylphosphonate linkages were examined for their ability to inhibit human immunodeficiency virus (HIV)-induced syncytium formation and...
Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer.
Cardullo, R A, Agrawal, S, Flores, C, Zamecnik, P C, Wolf, D E
Three approaches were used to study hybridization of complementary oligodeoxynucleotides by nonradiative fluorescence resonance energy transfer. (i) Fluorescein (donor) and rhodamine (acceptor) were...
Agrawal, S, Ikeuchi, T, Sun, D, Sarin, P S, Konopka, A, Maizel, J, ...
Antisense oligodeoxynucleotides, both the phosphorothioate analogues and unmodified oligomers of the same sequence, inhibit replication and expression of human immunodeficiency virus already growing...
Oligonucleotides that can hybridize to single-stranded complementary polypurine nucleic acid targets by Watson-Crick base pairing as well as by Hoogsteen base pairing, referred to here as foldback...
Sequence identity of the n-1 product of a synthetic oligonucleotide.
Temsamani, J, Kubert, M, Agrawal, S
After synthesis and purification of an oligonucleotide, the final product usually contains a low level of n-1 congeneric species. We have sequenced the n-1 population of a 25mer phosphodiester...
Kandimalla, E R, Manning, A, Lathan, C, Byrn, R A, Agrawal, S
Short oligonucleotides that can bind to adjacent sites on target mRNA sequences are designed and evaluated for their binding affinity and biological activity. Sequence-specific binding of short...
Kandimalla, E R, Manning, A N, Venkataraman, G, Sasisekharan, V, Agrawal, S
Foldback triplex-forming oligonucleotides (FTFOs) that contain an abasic linker, [2-(4-aminobutyr-1-yl)-1,3-propanediol] (APD linker), in the Hoogsteen domain against pyrimidine bases of a C:G and a...
Self-stabilized antisense oligodeoxynucleotide phosphorothioates: properties and anti-HIV activity.
Tang, J Y, Temsamani, J, Agrawal, S
A new class of oligodeoxyribonucleotides has been designed, referred to here as 'self-stabilized' oligonucleotides. These oligonucleotides have hairpin loop structures at their 3' ends, and show...
Agrawal, S, Christodoulou, C, Gait, M J
The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic...