Gaspar, Y., Nam, J., Schultz, Carolyn Jane, Lee, L-Y., Gilson, P. R., Gelvin, S. B., ...
© 2004 American Society of Plant Biologists
Gaspar, Y., Nam, J., Schultz, Carolyn Jane, Lee, L-Y., Gilson, P. R., Gelvin, S. B., ...
© 2004 American Society of Plant Biologists
He, S, Abad, A R, Gelvin, S B, Mackenzie, S A
In higher plants, dominant mitochondrial mutations are associated with pollen sterility. This phenomenon is known as cytoplasmic male sterility (CMS). It is thought that the disruption in pollen...
Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells.
Yusibov, V M, Steck, T R, Gupta, V, Gelvin, S B
During the inception of crown gall tumorigenesis, the transferred DNA (T-DNA) is processed from the Ti (tumor inducing) plasmid of Agrobacterium tumefaciens and is transferred to plant cells. T-DNA...
Nam, J, Matthysse, A G, Gelvin, S B
We show that among ecotypes of Arabidopsis, there is considerable variation in their susceptibility to crown gall disease. Differences in susceptibility are heritable and, in one ecotype, segregate...
Gray, J., Gelvin, S. B., Meilan, R., Morris, R. O.
Even in the absence of the classical Ti plasmid-encoded cytokinin biosynthetic genes ipt and tzs, Agrobacterium tumefaciens strains still release significant amounts of the cytokinin...
Multiple domains exist within the upstream activator sequence of the octopine synthase gene.
It is known that a 16-base pair palindrome (ACGTAAGCGCTTACGT) located upstream of the ocs gene can activate a maize adh1 promoter in a transient expression system [Ellis et al. (1987). EMBO J. 6,...
Kononowicz, H, Wang, Y E, Habeck, L L, Gelvin, S B
Previous work has shown that the octopine synthase (ocs) gene encoded by the Agrobacterium tumefaciens Ti-plasmid contains an upstream activating sequence necessary for its expression in plant cells....
Early transcription of Agrobacterium T-DNA genes in tobacco and maize.
Narasimhulu, S B, Deng, X B, Sarria, R, Gelvin, S B
We developed a sensitive procedure to investigate the kinetics of transcription of an Agrobacterium tumefaciens transferred (T)-DNA-encoded beta-glucuronidase gusA (uidA) gene soon after infection of...
Mutation of the miaA gene of Agrobacterium tumefaciens results in reduced vir gene expression.
vir regulon expression in Agrobacterium tumefaciens involves both chromosome- and Ti-plasmid-encoded gene products. We have isolated and characterized a new chromosomal gene that when mutated results...
Transport of nonmetabolizable opines by Agrobacterium tumefaciens.
Krishnan, M, Burgner, J W, Chilton, W S, Gelvin, S B
We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All...
Rong, L J, Karcher, S J, Gelvin, S B
picA is an Agrobacterium tumefaciens chromosomal locus, identified by Mu d11681 mutagenesis, that is inducible by certain acidic polysaccharides found in carrot root extract. Cloning and genetic...
vir genes influence conjugal transfer of the Ti plasmid of Agrobacterium tumefaciens.
Mutation of the genes virA, virB, virC, and virG of the Agrobacterium tumefaciens octopine-type Ti plasmid pTiR10 was found to cause a 100- to 10,000-fold decrease in the frequency of conjugal...
Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid.
Veluthambi, K, Krishnan, M, Gould, J H, Smith, R H, Gelvin, S B
Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine,...
Chang, C C, Jayaswal, R K, Chen, C M, Gelvin, S B
Octopine synthase encoded by the T-DNA (transferred DNA) locus ocs synthesizes N2-(D-1-carboxyethyl)-L-amino acids in octopine-type crown gall tumors. So far, derivatives of only basic amino acids...
Veluthambi, K, Ream, W, Gelvin, S B
Agrobacterium tumefaciens transfers the T-DNA portion of its Ti plasmid to the nuclear genome of plant cells. Upon cocultivation of A. tumefaciens A348 with regenerating tobacco leaf protoplasts, six...
Jayaswal, R K, Veluthambi, K, Gelvin, S B, Slightom, J L
The virD locus of Agrobacterium tumefaciens Ti plasmid pTiA6 was sequenced. Computer analysis of the sequence indicated five possible open reading frames (ORFs) within this locus. Two additional ORFs...
Petunia protoplasts were infected with the virulent Agrobacterium tumefaciens strain A348 or the avirulent strain A136 (lacking a Ti plasmid). The infection process was stopped at various time...
Structure of the octopine synthase upstream activator sequence.
We have identified a transcriptional activating element within the 5' flanking sequence of the Agrobacterium tumefaciens octopine synthase (ocs) gene that is necessary for ocs expression in...
Veluthambi, K., Jayaswal, R. K., Gelvin, S. B.
Agrobacterium tumefaciens transfers the T-DNA portion of its Ti plasmid to the nuclear genome of plant cells. Upon cocultivation of A. tumefaciens strain A348 with regenerating tobacco leaf...
Methylation of the T-DNA in Agrobacterium tumefaciens and in several crown gall tumors.
Gelvin, S B, Karcher, S J, DiRita, V J
Methylation of the T-DNA in Agrobacterium tumefaciens and in four octopine-type (A6S/2, E9, 15955/1, 15955/01) and one nopaline-type (HT37#15) crown gall tumors was investigated using the...
Gelvin, S B, Thomashow, M F, McPherson, J C, Gordon, M P, Nester, E W
The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors were determined by blot hybridization. At least six discrete...
Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites,...
picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome.
Rong, L, Karcher, S J, O'Neal, K, Hawes, M C, Yerkes, C D, Jayaswal, R K, ...
We used the transposon Mu dI1681 to identify genes on the Agrobacterium tumefaciens chromosome that are inducible by extracts from carrot roots. One such locus (picA, for plant inducible...
He, S, Abad, A R, Gelvin, S B, Mackenzie, S A
In higher plants, dominant mitochondrial mutations are associated with pollen sterility. This phenomenon is known as cytoplasmic male sterility (CMS). It is thought that the disruption in pollen...
Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells.
Yusibov, V M, Steck, T R, Gupta, V, Gelvin, S B
During the inception of crown gall tumorigenesis, the transferred DNA (T-DNA) is processed from the Ti (tumor inducing) plasmid of Agrobacterium tumefaciens and is transferred to plant cells. T-DNA...
Nam, J, Matthysse, A G, Gelvin, S B
We show that among ecotypes of Arabidopsis, there is considerable variation in their susceptibility to crown gall disease. Differences in susceptibility are heritable and, in one ecotype, segregate...
Gray, J., Gelvin, S. B., Meilan, R., Morris, R. O.
Even in the absence of the classical Ti plasmid-encoded cytokinin biosynthetic genes ipt and tzs, Agrobacterium tumefaciens strains still release significant amounts of the cytokinin...
Multiple domains exist within the upstream activator sequence of the octopine synthase gene.
It is known that a 16-base pair palindrome (ACGTAAGCGCTTACGT) located upstream of the ocs gene can activate a maize adh1 promoter in a transient expression system [Ellis et al. (1987). EMBO J. 6,...
Kononowicz, H, Wang, Y E, Habeck, L L, Gelvin, S B
Previous work has shown that the octopine synthase (ocs) gene encoded by the Agrobacterium tumefaciens Ti-plasmid contains an upstream activating sequence necessary for its expression in plant cells....
Early transcription of Agrobacterium T-DNA genes in tobacco and maize.
Narasimhulu, S B, Deng, X B, Sarria, R, Gelvin, S B
We developed a sensitive procedure to investigate the kinetics of transcription of an Agrobacterium tumefaciens transferred (T)-DNA-encoded beta-glucuronidase gusA (uidA) gene soon after infection of...
Mutation of the miaA gene of Agrobacterium tumefaciens results in reduced vir gene expression.
vir regulon expression in Agrobacterium tumefaciens involves both chromosome- and Ti-plasmid-encoded gene products. We have isolated and characterized a new chromosomal gene that when mutated results...
Transport of nonmetabolizable opines by Agrobacterium tumefaciens.
Krishnan, M, Burgner, J W, Chilton, W S, Gelvin, S B
We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All...
Rong, L J, Karcher, S J, Gelvin, S B
picA is an Agrobacterium tumefaciens chromosomal locus, identified by Mu d11681 mutagenesis, that is inducible by certain acidic polysaccharides found in carrot root extract. Cloning and genetic...
vir genes influence conjugal transfer of the Ti plasmid of Agrobacterium tumefaciens.
Mutation of the genes virA, virB, virC, and virG of the Agrobacterium tumefaciens octopine-type Ti plasmid pTiR10 was found to cause a 100- to 10,000-fold decrease in the frequency of conjugal...
Opines stimulate induction of the vir genes of the Agrobacterium tumefaciens Ti plasmid.
Veluthambi, K, Krishnan, M, Gould, J H, Smith, R H, Gelvin, S B
Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine,...
Chang, C C, Jayaswal, R K, Chen, C M, Gelvin, S B
Octopine synthase encoded by the T-DNA (transferred DNA) locus ocs synthesizes N2-(D-1-carboxyethyl)-L-amino acids in octopine-type crown gall tumors. So far, derivatives of only basic amino acids...
Veluthambi, K, Ream, W, Gelvin, S B
Agrobacterium tumefaciens transfers the T-DNA portion of its Ti plasmid to the nuclear genome of plant cells. Upon cocultivation of A. tumefaciens A348 with regenerating tobacco leaf protoplasts, six...
Jayaswal, R K, Veluthambi, K, Gelvin, S B, Slightom, J L
The virD locus of Agrobacterium tumefaciens Ti plasmid pTiA6 was sequenced. Computer analysis of the sequence indicated five possible open reading frames (ORFs) within this locus. Two additional ORFs...
Petunia protoplasts were infected with the virulent Agrobacterium tumefaciens strain A348 or the avirulent strain A136 (lacking a Ti plasmid). The infection process was stopped at various time...
Structure of the octopine synthase upstream activator sequence.
We have identified a transcriptional activating element within the 5' flanking sequence of the Agrobacterium tumefaciens octopine synthase (ocs) gene that is necessary for ocs expression in...
Veluthambi, K., Jayaswal, R. K., Gelvin, S. B.
Agrobacterium tumefaciens transfers the T-DNA portion of its Ti plasmid to the nuclear genome of plant cells. Upon cocultivation of A. tumefaciens strain A348 with regenerating tobacco leaf...
Methylation of the T-DNA in Agrobacterium tumefaciens and in several crown gall tumors.
Gelvin, S B, Karcher, S J, DiRita, V J
Methylation of the T-DNA in Agrobacterium tumefaciens and in four octopine-type (A6S/2, E9, 15955/1, 15955/01) and one nopaline-type (HT37#15) crown gall tumors was investigated using the...
Gelvin, S B, Thomashow, M F, McPherson, J C, Gordon, M P, Nester, E W
The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors were determined by blot hybridization. At least six discrete...
Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites,...
picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome.
Rong, L, Karcher, S J, O'Neal, K, Hawes, M C, Yerkes, C D, Jayaswal, R K, ...
We used the transposon Mu dI1681 to identify genes on the Agrobacterium tumefaciens chromosome that are inducible by extracts from carrot roots. One such locus (picA, for plant inducible...