An accurate, simple colorimetric method for the determination of serum α-hydroxybutyric dehydrogenase activity is described. The method is suitable for routine laboratory use.
Localization of leucine aminopeptidase isoenzymes
A simple, sensitive staining procedure for the localization of leucine aminopeptidase isoenzymes after electrophoretic separation on cellulose acetate membranes is described. Separation of leucine...
Pancreatic exocrine function after truncal and highly selective vagotomy.
Mikhailidis, D P, Foo, Y, Ramdial, L, Kirk, R M, Rosalki, S B, Dandona, P
We have measured plasma glucose and immunoreactive trypsin concentrations and serum pancreatic amylase activities in single blood samples following truncal vagotomy (TV) in 13 patients and highly...
Intestinal origin alkaline phosphatase activity in plasma for differential diagnosis of jaundice.
Intestinal origin alkaline phosphatase activity (ALP) in plasma was measured by a sensitive immunocapture assay in 104 jaundiced patients--84 with intrahepatic and 20 with post-hepatic jaundice....
Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition.
L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The...
Serum glutamate dehydrogenase is not a reliable marker of liver cell necrosis in alcoholics.
Jenkins, W J, Rosalki, S B, Foo, Y, Scheuer, P J, Nemesanszky, E, Sherlock, S
Serum glutamate dehydrogenase (EC 1.4.1.3.) activity was measured in 73 hospital patients who had a history of chronic alcohol abuse and who all had a liver biopsy performed. High levels of serum GDH...
Karmen, C, Mayne, P D, Foo, A Y, Parbhoo, S, Rosalki, S B
We describe a simple, rapid, and reproducible ion exchange mini-column chromatographic method for the quantitative measurement of biliary alkaline phosphatase in plasma. We have used this method to...
Exocrine pancreatic function in diabetes mellitus.
Dandona, P, Freedman, D B, Foo, Y, Perkins, J, Katrak, A, Mikhailidis, D P, ...
An investigation of serum immunoreactive trypsin concentration and pancreatic isoamylase activity in patients with diabetes mellitus has shown that exocrine pancreatic deficit is maximal in insulin...
Rosalki, S B, Foo, A Y, Dooley, J S
AIMS--To consider a possible genetic origin for the persistent unexplained increase in plasma alkaline phosphatase (ALP) in five non-related patients referred over an 18 month period. METHODS--Plasma...
Collinson, P O, Ramhamadany, E M, Rosalki, S B, Joffe, J, Evans, D H, Fink, R S, ...
A prospective study was made of sequential changes in serum creatine kinase (CK) and CK-MB isoenzyme activity within the 12 hours following admission to the coronary care unit on 65 patients with...
Mayne, P D, Thakrar, S, Rosalki, S B, Foo, A Y, Parbhoo, S
Plasma alkaline phosphatase isoenzyme activities were determined in patients with breast cancer to diagnose and monitor bone and liver metastases. Bone alkaline phosphatase activity was increased in...
Collins, D, Goold, M F, Rosalki, S B, Mayne, P D, Foo, A Y
An assessment was made of the value of: (i) the demonstration of intestinal alkaline phosphatase in plasma for the differentiation of intrahepatic from post-hepatic jaundice in 122 jaundiced...
Wickramasinghe, S N, Marjot, D H, Rosalki, S B, Fink, R S
A strong and highly significant correlation was observed between serum aspartate transaminase (AST) activity and an index of the cytotoxic activity associated with serum proteins modified by...
Serum isoamylase activities in diabetes mellitus.
Foo, Y, Rosalki, S B, Ramdial, L, Mikhailidis, D, Dandona, P
Serum pancreatic isoamylase activities were used to assess exocrine pancreatic function in 39 patients with diabetes mellitus (21 on insulin, 12 on sulphonylureas, and six on biguanides or diet), and...
An accurate, simple colorimetric method for the determination of serum α-hydroxybutyric dehydrogenase activity is described. The method is suitable for routine laboratory use.
Localization of leucine aminopeptidase isoenzymes
A simple, sensitive staining procedure for the localization of leucine aminopeptidase isoenzymes after electrophoretic separation on cellulose acetate membranes is described. Separation of leucine...
Pancreatic exocrine function after truncal and highly selective vagotomy.
Mikhailidis, D P, Foo, Y, Ramdial, L, Kirk, R M, Rosalki, S B, Dandona, P
We have measured plasma glucose and immunoreactive trypsin concentrations and serum pancreatic amylase activities in single blood samples following truncal vagotomy (TV) in 13 patients and highly...
Intestinal origin alkaline phosphatase activity in plasma for differential diagnosis of jaundice.
Intestinal origin alkaline phosphatase activity (ALP) in plasma was measured by a sensitive immunocapture assay in 104 jaundiced patients--84 with intrahepatic and 20 with post-hepatic jaundice....
Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition.
L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The...
Serum glutamate dehydrogenase is not a reliable marker of liver cell necrosis in alcoholics.
Jenkins, W J, Rosalki, S B, Foo, Y, Scheuer, P J, Nemesanszky, E, Sherlock, S
Serum glutamate dehydrogenase (EC 1.4.1.3.) activity was measured in 73 hospital patients who had a history of chronic alcohol abuse and who all had a liver biopsy performed. High levels of serum GDH...
Karmen, C, Mayne, P D, Foo, A Y, Parbhoo, S, Rosalki, S B
We describe a simple, rapid, and reproducible ion exchange mini-column chromatographic method for the quantitative measurement of biliary alkaline phosphatase in plasma. We have used this method to...
Exocrine pancreatic function in diabetes mellitus.
Dandona, P, Freedman, D B, Foo, Y, Perkins, J, Katrak, A, Mikhailidis, D P, ...
An investigation of serum immunoreactive trypsin concentration and pancreatic isoamylase activity in patients with diabetes mellitus has shown that exocrine pancreatic deficit is maximal in insulin...
Rosalki, S B, Foo, A Y, Dooley, J S
AIMS--To consider a possible genetic origin for the persistent unexplained increase in plasma alkaline phosphatase (ALP) in five non-related patients referred over an 18 month period. METHODS--Plasma...
Collinson, P O, Ramhamadany, E M, Rosalki, S B, Joffe, J, Evans, D H, Fink, R S, ...
A prospective study was made of sequential changes in serum creatine kinase (CK) and CK-MB isoenzyme activity within the 12 hours following admission to the coronary care unit on 65 patients with...
Mayne, P D, Thakrar, S, Rosalki, S B, Foo, A Y, Parbhoo, S
Plasma alkaline phosphatase isoenzyme activities were determined in patients with breast cancer to diagnose and monitor bone and liver metastases. Bone alkaline phosphatase activity was increased in...
Collins, D, Goold, M F, Rosalki, S B, Mayne, P D, Foo, A Y
An assessment was made of the value of: (i) the demonstration of intestinal alkaline phosphatase in plasma for the differentiation of intrahepatic from post-hepatic jaundice in 122 jaundiced...
Wickramasinghe, S N, Marjot, D H, Rosalki, S B, Fink, R S
A strong and highly significant correlation was observed between serum aspartate transaminase (AST) activity and an index of the cytotoxic activity associated with serum proteins modified by...
Serum isoamylase activities in diabetes mellitus.
Foo, Y, Rosalki, S B, Ramdial, L, Mikhailidis, D, Dandona, P
Serum pancreatic isoamylase activities were used to assess exocrine pancreatic function in 39 patients with diabetes mellitus (21 on insulin, 12 on sulphonylureas, and six on biguanides or diet), and...
Fraser, A G, Rosalki, S B, Gamble, G D, Pounder, R E
1. The magnitude of the variability of ethanol absorption is an important factor for studies that seek to determine the significance of potential interactions between ethanol and drugs. The aim of...