Lauer, J.L., Shohet, J.L., Albrecht, R.M., Pratoomtong, C., Murugesan, R., Esnault, S., ...
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying...
Thevenin, B J, Periasamy, N, Shohet, S B, Verkman, A S
Interactions between the erythrocyte membrane and its skeleton are mediated primarily by binding of cytoskeletal components to a conformationally sensitive structure, the cytoplasmic domain of band 3...
Galili, U, Mandrell, R E, Hamadeh, R M, Shohet, S B, Griffiss, J M
Anti-alpha-galactosyl immunoglobulin G (anti-Gal) is a natural antibody present in unusually high amounts in human sera. It constitutes as much as 1% of circulating immunoglobulin G in humans and...
Galili, U, Clark, M R, Shohet, S B, Buehler, J, Macher, B A
Anti-Gal is a natural antibody, which constitutes as much as 1% of circulating IgG in humans and displays a distinct specificity for the structure Gal alpha 1----3Gal. This glycosidic structure has...
Hydration of sickle cells using the sodium ionophore Monensin. A model for therapy.
Clark, M R, Mohandas, N, Shohet, S B
Mean cell hemoglobin concentration (MCHC) is thought to have an important influence in sickle cell disease, both through the strong dependence of sickling rates on hemoglobin S concentration, and...
Molecular and functional changes in spectrin from patients with hereditary pyropoikilocytosis.
Knowles, W J, Morrow, J S, Speicher, D W, Zarkowsky, H S, Mohandas, N, Mentzer, W C, ...
The structural and functional properties of spectrin from normal and hereditary pyropoikilocytosis (HPP) donors from the two unrelated families were studied. The structural domains of the spectrin...
Tchernia, G, Mohandas, N, Shohet, S B
Erythrocytes from three patients with severe hemolytic anemia, marked erythrocyte fragmentation, and elliptocytic poikilocytosis, were studied in terms of both their membrane protein composition and...
Deformability of oxygenated irreversibly sickled cells.
Clark, M R, Mohandas, N, Shohet, S B
The deformability characteristics of isolated subpopulations of irreversibly sickled cells (ISC) have been studied in an ektacytometer. Analysis of laser diffraction patterns of well-oxygenated cells...
Analysis of factors regulating erythrocyte deformability.
Mohandas, N, Clark, M R, Jacobs, M S, Shohet, S B
Using a laser diffraction technique, we have studied factors that influence the deformability of erythrocytes. Variations in suspending medium osmolality and applied shear stress were employed to...
Monovalent cation transport in irreversibly sickled cells.
Clark, M R, Morrison, C E, Shohet, S B
Using discontinuous density gradients of Stractan II, we have separated sickle cell blood into discrete subpopulations of reticulocytes, mature discoid cells, and irreversibly sickled cells (ISCs)....
Reconstitution of spectrin-deficient, spherocytic mouse erythrocyte membranes.
To study directly the role of spectrin in erythrocyte membrane function, we have designed a reconstituted membrane system using erythrocyte membranes from spectrin-deficient mice and purified...
Conboy, J, Kan, Y W, Shohet, S B, Mohandas, N
Protein 4.1 is an important structural protein that is expressed in erythroid and in a variety of non-erythroid tissues. In mammalian erythrocytes, it plays a key role in regulating membrane physical...
Stabilization of erythrocyte membranes by polyamines.
Ballas, S K, Mohandas, N, Marton, L J, Shohet, S B
Using a laser diffraction technique, we have studied the effects of putrescine, spermidine, and spermine, the three physiologic polyamines, on the deformability and mechanical stability of human...
Lipid Peroxidation in the Killing of Phagocytized Pneumococci
Shohet, S. B., Pitt, J., Baehner, R. L., Poplack, D. G.
To directly examine the role of hydrogen peroxide in the killing of bacteria after ingestion by granulocytes, we have studied some of the events of phagocytosis of a mutant strain of pneumococci...
Galili, U, Clark, M R, Shohet, S B
A large proportion of sickle erythrocytes is removed from the circulation by the macrophages of the reticuloendothelial system. In view of the proposed role for natural antibodies in the destruction...
Chasis, J A, Mohandas, N, Shohet, S B
Erythrocyte skeletal proteins are known to play an important role in determining membrane deformability. In order to see whether transmembrane proteins also influence deformability and, if so,...
Bicknese, S, Periasamy, N, Shohet, S B, Verkman, A S
The purpose of this study was to determine whether the unique physical milieu just beneath the cell plasma membrane influences the rheology of fluid-phase cytoplasm. Cytoplasmic viscosity was...
Thevenin, B J, Bicknese, S E, Park, J, Verkman, A S, Shohet, S B
Single-photon radioluminescence (SPR), the excitation of fluorophores by short-range beta-decay electrons, was developed for the measurement of submicroscopic distances. The cytoplasmic domain of...
Single photon radioluminescence. I. Theory and spectroscopic properties.
Bicknese, S, Shahrokh, Z, Shohet, S B, Verkman, A S
The excitation of a fluorescent molecule by a beta-decay electron (radioluminescence) depends upon the electron energy, the distance between radioactive 'donor' and fluorescent 'acceptor', and the...
Single photon radioluminescence. II. Signal detection and biological applications.
Shahrokh, Z, Bicknese, S, Shohet, S B, Verkman, A S
A quantitative theory for excitation of fluorescent molecules by beta decay electrons is reported in the accompanying manuscript; experimental detection methods and biological applications are...
Calculation of resonance energy transfer in crowded biological membranes.
Zimet, D B, Thevenin, B J, Verkman, A S, Shohet, S B, Abney, J R
Analytical and numerical models were developed to describe fluorescence resonance energy transfer (RET) in crowded biological membranes. It was assumed that fluorescent donors were linked to membrane...
Thevenin, B J, Periasamy, N, Shohet, S B, Verkman, A S
Interactions between the erythrocyte membrane and its skeleton are mediated primarily by binding of cytoskeletal components to a conformationally sensitive structure, the cytoplasmic domain of band 3...
Galili, U, Mandrell, R E, Hamadeh, R M, Shohet, S B, Griffiss, J M
Anti-alpha-galactosyl immunoglobulin G (anti-Gal) is a natural antibody present in unusually high amounts in human sera. It constitutes as much as 1% of circulating immunoglobulin G in humans and...
Galili, U, Clark, M R, Shohet, S B, Buehler, J, Macher, B A
Anti-Gal is a natural antibody, which constitutes as much as 1% of circulating IgG in humans and displays a distinct specificity for the structure Gal alpha 1----3Gal. This glycosidic structure has...
Hydration of sickle cells using the sodium ionophore Monensin. A model for therapy.
Clark, M R, Mohandas, N, Shohet, S B
Mean cell hemoglobin concentration (MCHC) is thought to have an important influence in sickle cell disease, both through the strong dependence of sickling rates on hemoglobin S concentration, and...
Molecular and functional changes in spectrin from patients with hereditary pyropoikilocytosis.
Knowles, W J, Morrow, J S, Speicher, D W, Zarkowsky, H S, Mohandas, N, Mentzer, W C, ...
The structural and functional properties of spectrin from normal and hereditary pyropoikilocytosis (HPP) donors from the two unrelated families were studied. The structural domains of the spectrin...
Tchernia, G, Mohandas, N, Shohet, S B
Erythrocytes from three patients with severe hemolytic anemia, marked erythrocyte fragmentation, and elliptocytic poikilocytosis, were studied in terms of both their membrane protein composition and...
Deformability of oxygenated irreversibly sickled cells.
Clark, M R, Mohandas, N, Shohet, S B
The deformability characteristics of isolated subpopulations of irreversibly sickled cells (ISC) have been studied in an ektacytometer. Analysis of laser diffraction patterns of well-oxygenated cells...
Analysis of factors regulating erythrocyte deformability.
Mohandas, N, Clark, M R, Jacobs, M S, Shohet, S B
Using a laser diffraction technique, we have studied factors that influence the deformability of erythrocytes. Variations in suspending medium osmolality and applied shear stress were employed to...
Monovalent cation transport in irreversibly sickled cells.
Clark, M R, Morrison, C E, Shohet, S B
Using discontinuous density gradients of Stractan II, we have separated sickle cell blood into discrete subpopulations of reticulocytes, mature discoid cells, and irreversibly sickled cells (ISCs)....
Reconstitution of spectrin-deficient, spherocytic mouse erythrocyte membranes.
To study directly the role of spectrin in erythrocyte membrane function, we have designed a reconstituted membrane system using erythrocyte membranes from spectrin-deficient mice and purified...
Conboy, J, Kan, Y W, Shohet, S B, Mohandas, N
Protein 4.1 is an important structural protein that is expressed in erythroid and in a variety of non-erythroid tissues. In mammalian erythrocytes, it plays a key role in regulating membrane physical...
Stabilization of erythrocyte membranes by polyamines.
Ballas, S K, Mohandas, N, Marton, L J, Shohet, S B
Using a laser diffraction technique, we have studied the effects of putrescine, spermidine, and spermine, the three physiologic polyamines, on the deformability and mechanical stability of human...
Lipid Peroxidation in the Killing of Phagocytized Pneumococci
Shohet, S. B., Pitt, J., Baehner, R. L., Poplack, D. G.
To directly examine the role of hydrogen peroxide in the killing of bacteria after ingestion by granulocytes, we have studied some of the events of phagocytosis of a mutant strain of pneumococci...
Galili, U, Clark, M R, Shohet, S B
A large proportion of sickle erythrocytes is removed from the circulation by the macrophages of the reticuloendothelial system. In view of the proposed role for natural antibodies in the destruction...
Chasis, J A, Mohandas, N, Shohet, S B
Erythrocyte skeletal proteins are known to play an important role in determining membrane deformability. In order to see whether transmembrane proteins also influence deformability and, if so,...
Bicknese, S, Periasamy, N, Shohet, S B, Verkman, A S
The purpose of this study was to determine whether the unique physical milieu just beneath the cell plasma membrane influences the rheology of fluid-phase cytoplasm. Cytoplasmic viscosity was...
Thevenin, B J, Bicknese, S E, Park, J, Verkman, A S, Shohet, S B
Single-photon radioluminescence (SPR), the excitation of fluorophores by short-range beta-decay electrons, was developed for the measurement of submicroscopic distances. The cytoplasmic domain of...
Single photon radioluminescence. I. Theory and spectroscopic properties.
Bicknese, S, Shahrokh, Z, Shohet, S B, Verkman, A S
The excitation of a fluorescent molecule by a beta-decay electron (radioluminescence) depends upon the electron energy, the distance between radioactive 'donor' and fluorescent 'acceptor', and the...
Single photon radioluminescence. II. Signal detection and biological applications.
Shahrokh, Z, Bicknese, S, Shohet, S B, Verkman, A S
A quantitative theory for excitation of fluorescent molecules by beta decay electrons is reported in the accompanying manuscript; experimental detection methods and biological applications are...
Calculation of resonance energy transfer in crowded biological membranes.
Zimet, D B, Thevenin, B J, Verkman, A S, Shohet, S B, Abney, J R
Analytical and numerical models were developed to describe fluorescence resonance energy transfer (RET) in crowded biological membranes. It was assumed that fluorescent donors were linked to membrane...