The Bacillus secretion stress response is an indicator for α-amylase production levels (2004)
Westers, H., Darmon, E., Zanen, G., Kuipers, O.P., Bron, S., ...
Aims: Overproduced α-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously,...
Tjalsma, H., Koetje, E.J., Kiewiet, R., Kuipers, O.P., Kolkman, M., Laan, J. Van Der, ...
Aim: Engineering of Rap-Phr quorum-sensing systems of Bacillus subtilis and subsequent evaluation of the transcription of the aprE gene, encoding a major extracellular alkaline protease. Methods and...
Dorenbos, R., Stein, T., Kabel, J., Bruand, C., Bolhuis, A., Bron, S., ...
Membrane topology of the Streptomyces lividans type I signal peptidases (2001)
Schacht, S, Parro, V, ...
Most bacterial membranes contain one or two type I signal peptidases (SPases) for the removal of signal peptides from export proteins. For Streptomyces lividans, four different type I SPases (denoted...
Tjalsma, H, Van Dijl, J M, Bron, S, ...
Type I signal peptidases (SPases) are a widespread family of enzymes which remove signal peptides from proteins translocated across cellular membranes. Here, we report the first isolation of a gene...
The complete genome sequence of the Gram-positive bacterium Bacillus subtilis (1997)
Kunst, F., Ogasawara, N., Moszer, I., Albertini, A.M., Alloni, G., Azevedo, V., ...
Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are...
The complete genome sequence of the gram-positive bacterium Bacillus subtilis. (1997)
Kunst, F, Ogasawara, N, Moszer, I, Albertini, A M, Alloni, G, Azevedo, V, ...
Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are...
The Complete Genome Sequence Of The Gram-Positive Bacterium Bacillus Subtilis (1997)
Kunst, F., Ogasawara, N., Moszer, I., Albertini, Am., Alloni, G., Azevedo, V., ...
It was found that combinations of shear creep and torsional oscillations, applied simultaneously to adhesive joints at temperatures within the glassy range of the adhesive, led to strengthening of...
Dijkhuizen, L., Harder, W., Boer, L. De, Boven, A. Van, Clement, W., Bron, S., ...
The inability of Hyphomicrobium X to grow on compounds such as pyruvate and succinate is most likely due to the absence of a functional pyruvate dehydrogenase (PDH) complex. Further support for this...
Van Roosmalen, M. L., Jongbloed, J. D. H., Kuipers, A., Venema, G., Bron, S., Van Dijl, J. M.
Soluble forms of Bacillus signal peptidases which lack their unique amino-terminal membrane anchor are prone to degradation, which precludes their high-level production in the cytoplasm of...
Meima, R, Haan, G J, Venema, G, Bron, S, De Jong, S
Previous work in our group indicated that structural plasmid instability in Bacillus subtilis is often caused by illegitimate recombination between non-repeated sequences, characterized by a...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Cordes, C, Meima, R, Twiest, B, Kazemier, B, Venema, G, Van Dijl, J M, ...
The rolling-circle plasmid pGP1 was used to study the effects of the expression of a plasmid-specified exported protein on structural plasmid stability in Bacillus subtilis. pGP1 contains a fusion...
Meima, R, Haijema, B J, Dijkstra, H, Haan, G J, Venema, G, Bron, S
The structural stability of plasmid pGP1, which encodes a fusion between the penicillinase gene (penP) of Bacillus licheniformis and the Escherichia coli lacZ gene, was investigated in Bacillus...
Wösten, H A, Bron, S, Oskam, L, Venema, G
pMV158, a non-self-transmissible plasmid encoding tetracycline resistance, was conjugally transferred from Enterococcus faecalis JH203 to Lactococcus lactis subsp. lactis IL1403. This transfer...
Construction and use of signal sequence selection vectors in Escherichia coli and Bacillus subtilis.
Smith, H, Bron, S, Van Ee, J, Venema, G
To study the diversity and efficiency of signal peptides for secreted proteins in gram-positive bacteria, two plasmid vectors were constructed which were used to probe for export signal-coding...
Smith, H, Wiersma, K, Venema, G, Bron, S
A 75,000-dalton protein complex purified from membranes of competent Bacillus subtilis cells was previously shown to be involved in both binding and entry of donor DNA during transformation. The...
Smith, H, Wiersma, K, Bron, S, Venema, G
In DNA binding-deficient mutants of Bacillus subtilis a competence-specific protein with a subunit molecular weight of 18,000 was absent. The native protein containing this subunit was purified from...
Smith, H, Wiersma, K, Venema, G, Bron, S
A 75,000-dalton protein complex involved in DNA binding during transformation was purified from membranes of competent Bacillus subtilis cells. Previous results (Smith et al., J. Bacteriol....
Transformation in Bacillus subtilis: properties of DNA-binding-deficient mutants.
Transformation-deficient mutants of Bacillus subtilis were selected after replica plating on agar plates containing transforming DNA. Out of 24 mutants tested, 3 showed highly reduced abilities to...
Resistance of bacteriophage H1 to restriction and modification by Bacillus subtilis R.
H1, a 5-hydroxymethyluracil (HMU)-containing Bacillus subtilis bacteriophage, was neither restricted nor modified upon infection of B. subtilis R cells. In vitro, H1 DNA was not restricted by BsuR...
The role of homology between donor and recipient DNAs in the protection of transfecting DNA against restriction by competent Bacillus subtilis R cells was studied under marker rescue conditions with...
Characterization of single strand origins of cryptic rolling-circle plasmids from Bacillus subtilis.
Meijer, W J, Venema, G, Bron, S
In this paper we describe the isolation and characterization of single strand origins (SSOs) of several cryptic Bacillus subtilis plasmids which use the rolling-circle mechanism of replication. The...
Meijer, W J, De Boer, A J, Van Tongeren, S, Venema, G, Bron, S
A 3.1 kb fragment of the large (approximately 55 kb) Bacillus subtilis plasmid pLS20 containing all the information for autonomous replication was cloned and sequenced. In contrast to the parental...
Plasmids pMV158 and pTB913, originating from Streptococcus agalactiae and a thermophilic Bacillus respectively, were sequenced to completion. Both contained a BA3-type minus origin of replication and...
In Vitro Excision-Repair of Ultraviolet-Irradiated Transforming DNA from Bacillus subtilis
Heijneker, H. L., Pannekoek, H., Oosterbaan, R. A., Pouwels, P. H., Bron, S., Arwert, F., ...
The biological activity of UV-inactivated Bacillus subtilis DNA is partly restored after incubation with a UV-specific endonuclease from Micrococcus lutens in conjunction with DNA polymerase and DNA...
Breakage--reunion and copy choice mechanisms of recombination between short homologous sequences.
Brunier, D, Peeters, B P, Bron, S, Ehrlich, S D
To study recombination between short homologous sequences in Escherichia coli we constructed plasmids composed of the pBR322 replicon, M13 replication origin and a recombination unit inserted within...
Van Dijl, J M, De Jong, A, Vehmaanperä, J, Venema, G, Bron, S
Signal peptidases (SPases) remove signal peptides from secretory proteins. The sipS (signal peptidase of subtilis) gene, which encodes an SPase of Bacillus subtilis, was cloned in Escherichia coli...
Van Roosmalen, M. L., Jongbloed, J. D. H., Kuipers, A., Venema, G., Bron, S., Van Dijl, J. M.
Soluble forms of Bacillus signal peptidases which lack their unique amino-terminal membrane anchor are prone to degradation, which precludes their high-level production in the cytoplasm of...
Meima, R, Haan, G J, Venema, G, Bron, S, De Jong, S
Previous work in our group indicated that structural plasmid instability in Bacillus subtilis is often caused by illegitimate recombination between non-repeated sequences, characterized by a...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Cordes, C, Meima, R, Twiest, B, Kazemier, B, Venema, G, Van Dijl, J M, ...
The rolling-circle plasmid pGP1 was used to study the effects of the expression of a plasmid-specified exported protein on structural plasmid stability in Bacillus subtilis. pGP1 contains a fusion...
Meima, R, Haijema, B J, Dijkstra, H, Haan, G J, Venema, G, Bron, S
The structural stability of plasmid pGP1, which encodes a fusion between the penicillinase gene (penP) of Bacillus licheniformis and the Escherichia coli lacZ gene, was investigated in Bacillus...
Wösten, H A, Bron, S, Oskam, L, Venema, G
pMV158, a non-self-transmissible plasmid encoding tetracycline resistance, was conjugally transferred from Enterococcus faecalis JH203 to Lactococcus lactis subsp. lactis IL1403. This transfer...
Construction and use of signal sequence selection vectors in Escherichia coli and Bacillus subtilis.
Smith, H, Bron, S, Van Ee, J, Venema, G
To study the diversity and efficiency of signal peptides for secreted proteins in gram-positive bacteria, two plasmid vectors were constructed which were used to probe for export signal-coding...
Smith, H, Wiersma, K, Venema, G, Bron, S
A 75,000-dalton protein complex purified from membranes of competent Bacillus subtilis cells was previously shown to be involved in both binding and entry of donor DNA during transformation. The...
Smith, H, Wiersma, K, Bron, S, Venema, G
In DNA binding-deficient mutants of Bacillus subtilis a competence-specific protein with a subunit molecular weight of 18,000 was absent. The native protein containing this subunit was purified from...
Smith, H, Wiersma, K, Venema, G, Bron, S
A 75,000-dalton protein complex involved in DNA binding during transformation was purified from membranes of competent Bacillus subtilis cells. Previous results (Smith et al., J. Bacteriol....
Transformation in Bacillus subtilis: properties of DNA-binding-deficient mutants.
Transformation-deficient mutants of Bacillus subtilis were selected after replica plating on agar plates containing transforming DNA. Out of 24 mutants tested, 3 showed highly reduced abilities to...
Resistance of bacteriophage H1 to restriction and modification by Bacillus subtilis R.
H1, a 5-hydroxymethyluracil (HMU)-containing Bacillus subtilis bacteriophage, was neither restricted nor modified upon infection of B. subtilis R cells. In vitro, H1 DNA was not restricted by BsuR...
The role of homology between donor and recipient DNAs in the protection of transfecting DNA against restriction by competent Bacillus subtilis R cells was studied under marker rescue conditions with...
Characterization of single strand origins of cryptic rolling-circle plasmids from Bacillus subtilis.
Meijer, W J, Venema, G, Bron, S
In this paper we describe the isolation and characterization of single strand origins (SSOs) of several cryptic Bacillus subtilis plasmids which use the rolling-circle mechanism of replication. The...
Meijer, W J, De Boer, A J, Van Tongeren, S, Venema, G, Bron, S
A 3.1 kb fragment of the large (approximately 55 kb) Bacillus subtilis plasmid pLS20 containing all the information for autonomous replication was cloned and sequenced. In contrast to the parental...
Plasmids pMV158 and pTB913, originating from Streptococcus agalactiae and a thermophilic Bacillus respectively, were sequenced to completion. Both contained a BA3-type minus origin of replication and...
In Vitro Excision-Repair of Ultraviolet-Irradiated Transforming DNA from Bacillus subtilis
Heijneker, H. L., Pannekoek, H., Oosterbaan, R. A., Pouwels, P. H., Bron, S., Arwert, F., ...
The biological activity of UV-inactivated Bacillus subtilis DNA is partly restored after incubation with a UV-specific endonuclease from Micrococcus lutens in conjunction with DNA polymerase and DNA...
Breakage--reunion and copy choice mechanisms of recombination between short homologous sequences.
Brunier, D, Peeters, B P, Bron, S, Ehrlich, S D
To study recombination between short homologous sequences in Escherichia coli we constructed plasmids composed of the pBR322 replicon, M13 replication origin and a recombination unit inserted within...
Van Dijl, J M, De Jong, A, Vehmaanperä, J, Venema, G, Bron, S
Signal peptidases (SPases) remove signal peptides from secretory proteins. The sipS (signal peptidase of subtilis) gene, which encodes an SPase of Bacillus subtilis, was cloned in Escherichia coli...
The chemistry and enzymology of the type I signal peptidases.
Dalbey, R. E., Lively, M. O., Bron, S., Van Dijl, J. M.
The discovery that proteins exported from the cytoplasm are typically synthesized as larger precursors with cleavable signal peptides has focused interest on the peptidases that remove the signal...