Scientific Commons: S. G. Rhee

Diminished Immunoreactivity of {gamma}-Glutamylcysteine Synthetase in the Airways of (2002)

Petra Pietarinen-runtti, Essi Lakari, Kari O. Raivio, Vuokko L. Kinnula, V L Kinnula, ...

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Identification of components of a phosphoinositide signaling pathway in retinal rod outer segments

Rhee, S. G., Schoen, T., Chader, G. J., ...

Phototransduction in retinal rods involves a G protein-coupled signaling cascade that leads to cGMP hydrolysis and the closure of cGMP-gated cation channels that are open in darkness, producing a...

Phospholipase C-gamma 1 can induce DNA synthesis by a mechanism independent of its lipase activity.

Smith, M R, Liu, Y L, Matthews, N T, Rhee, S G, Sung, W K, Kung, H F

Inositol phospholipid-specific phospholipase C (PLC) is involved in several signaling pathways leading to cellular growth and differentiation. Our previous studies reported the induction of DNA...

Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes.

Chae, H Z, Robison, K, Poole, L B, Church, G, Storz, G, Rhee, S G

A cDNA corresponding to a thiol-specific antioxidant enzyme (TSA) was isolated from a rat brain cDNA library with the use of antibodies to bovine TSA. The cDNA clone encoded an open reading frame...

Dimerization of thiol-specific antioxidant and the essential role of cysteine 47.

Chae, H Z, Uhm, T B, Rhee, S G

Thiol-specific antioxidant (TSA) from yeast contains cysteine residues at amino acid positions 47 and 170 but is not associated with obvious redox cofactors. These two cysteines are highly conserved...

Activation of rat brain phospholipase D by ADP-ribosylation factors 1,5, and 6: separation of ADP-ribosylation factor-dependent and oleate-dependent enzymes.

Massenburg, D, Han, J S, Liyanage, M, Patton, W A, Rhee, S G, Moss, J, ...

Two major forms of phospholipase D (PLD) activity, solubilized from rat brain membranes with Triton X-100, were separated by HPLC on a heparin-5PW column with buffer containing octyl glucoside. One...

Purification and characterization of recombinant G16 alpha from Sf9 cells: activation of purified phospholipase C isozymes by G-protein alpha subunits.

Kozasa, T, Hepler, J R, Smrcka, A V, Simon, M I, Rhee, S G, Sternweis, P C, ...

A cDNA encoding G16 alpha, the alpha subunit of a heterotrimeric guanine nucleotide-binding protein, was expressed in Sf9 cells using recombinant baculovirus. G16 alpha in membrane extracts of Sf9...

Tyrosine phosphorylation of phospholipase C-gamma 1 induced by cross-linking of the high-affinity or low-affinity Fc receptor for IgG in U937 cells.

Liao, F, Shin, H S, Rhee, S G

The human monocytic cell line U937 possesses two classes of the IgG Fc receptor (Fc gamma R), a high-affinity 72-kDa Fc gamma R (Fc gamma RI) and a low-affinity 40-kDa Fc gamma R (Fc gamma RII)....

Tyrosine phosphorylation of phospholipase C induced by membrane immunoglobulin in B lymphocytes.

Carter, R H, Park, D J, Rhee, S G, Fearon, D T

Ligation of membrane IgM on B lymphocytes causes activation of a protein-tyrosine kinase(s) (PTK) and of phospholipase C (PLC). To determine whether these are elements of a common signal-transduction...

CD3 stimulation causes phosphorylation of phospholipase C-gamma 1 on serine and tyrosine residues in a human T-cell line.

Park, D J, Rho, H W, Rhee, S G

The human T-cell line Jurkat was found to contain at least two immunologically distinct isoforms of inositol phospholipid-specific phospholipase C (PLC), PLC-beta 1 and PLC-gamma 1. Treatment of...

Immunocytochemical localization of phospholipase C-gamma in rat embryo fibroblasts.

McBride, K, Rhee, S G, Jaken, S

Rat embryo fibroblasts (REF52) exhibit a distinctive, transformation-sensitive distribution of alpha-protein kinase C (alpha-PKC). Receptor-mediated activation of phospholipase C (PLC)-gamma...

Selectivity of phospholipase C phosphorylation by the epidermal growth factor receptor, the insulin receptor, and their cytoplasmic domains.

Nishibe, S, Wahl, M I, Wedegaertner, P B, Kim, J W, Rhee, S G, Carpenter, G, ...

Phosphatidylinositol-specific phospholipase C isozyme gamma (PLC-gamma, Mr 145,000) is an excellent substrate for the epidermal growth factor (EGF) receptor both in vivo and in vitro. PLC-beta-1,...

Glutamine synthetase of Klebsiella aerogenes: properties of glnD mutants lacking uridylyltransferase.

Foor, F, Cedergren, R J, Streicher, S L, Rhee, S G, Magasanik, B

The glnD mutation of Klebsiella aerogenes is cotransducible by phage P1 with pan (requirement for pantothenate) and leads to a loss of uridylytransferase and uridylyl-removing enzyme, components of...

Mutations that alter the covalent modification of glutamine synthetase in Salmonella typhimurium.

Bancroft, S, Rhee, S G, Neumann, C, Kustu, S

glnD and glnE mutant strains of Salmonella typhimurium lack three of the four activities required for reversible covalent modification of glutamine synthetase (GS; EC 6.3.1.2). The glnD strains,...

Phospholipase C I and II brain isozymes: immunohistochemical localization in neuronal systems in rat brain.

Gerfen, C R, Choi, W C, Suh, P G, Rhee, S G

Two distinct inositol phospholipid-specific phospholipase C (PLC; phosphatidylcholine phosphatidohydrolase, EC 3.1.4.3) isozymes, PLC-I and PLC-II, have been purified and characterized from bovine...

Inositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products.

Suh, P G, Ryu, S H, Moon, K H, Suh, H W, Rhee, S G

Antibodies against an inositol phospholipid-specific phospholipase C purified from bovine brain were used to screen rat brain lambda gt11 expression cDNA libraries. Complete sequences of three cDNA...

Epidermal growth factor stimulates tyrosine phosphorylation of phospholipase C-II independently of receptor internalization and extracellular calcium.

Wahl, M I, Nishibe, S, Suh, P G, Rhee, S G, Carpenter, G

Epidermal growth factor (EGF) rapidly stimulates the formation of inositol 1,4,5-trisphosphate in a variety of cell types. Previously we have found that in intact cells stimulation of phospholipase C...

S-phase induction and transformation of quiescent NIH 3T3 cells by microinjection of phospholipase C.

Smith, M R, Ryu, S H, Suh, P G, Rhee, S G, Kung, H F

Two inositol phospholipid-specific phospholipase C (PLC) isozymes (PLC-I and -II) have been purified from bovine brain. When PLC-I or PLC-II was microinjected (100-700 micrograms/ml) into quiescent...

Induction of an antioxidant protein of Saccharomyces cerevisiae by O2, Fe3+, or 2-mercaptoethanol.

Kim, I H, Kim, K, Rhee, S G

A soluble 27-kDa protein from Saccharomyces cerevisiae specifically prevents the inactivation of various enzymes caused by a nonenzymatic Fe3+/O2/thiol mixed-function oxidation system but not by...

Platelet-derived growth factor (PDGF) binding promotes physical association of PDGF receptor with phospholipase C.

Kumjian, D A, Wahl, M I, Rhee, S G, Daniel, T O

Phospholipase C (PLC)-mediated production of inositol trisphosphate and diacylglycerol is stimulated by binding of platelet-derived growth factor (PDGF) to cell-surface receptors. Antibodies...

Phospholipase C associated with particulate fractions of bovine brain.

Lee, K Y, Ryu, S H, Suh, P G, Choi, W C, Rhee, S G

We previously reported that cytosolic fractions of bovine brain contain two immunologically distinct phosphoinositide-specific phospholipases C (PLCs), PLC-I and PLC-II. In this report the...

Bovine brain cytosol contains three immunologically distinct forms of inositolphospholipid-specific phospholipase C.

Ryu, S H, Suh, P G, Cho, K S, Lee, K Y, Rhee, S G

We previously reported that cytosolic fractions of bovine brain contain two immunologically distinct forms of phospholipase C (PLC), PLC-I and PLC-II. We now report the purification of another form...

Inositol polyphosphates are not increased by overexpression of Ins(1,4,5)P3 3-kinase but show cell-cycle dependent changes in growth factor-stimulated fibroblasts.

Balla, T, Sim, S S, Baukal, A J, Rhee, S G, Catt, K J

NIH 3T3 fibroblasts were stably transfected with rat brain inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) 3-kinase to explore the relationship between increased production of Ins(1,3,4,5)P4 and the...

Mechanistic studies of glutamine synthetase from Escherichia coli: kinetic evidence for two reaction intermediates in biosynthetic reaction.

Rhee, S G, Chock, P B

Fast reaction techniques were used to study the kinetics of protein fluorescence intensity changes that are associated with the reactions of unadenylylated Escherichia coli glutamine synthetase...

Anti-AMP antibody precipitation of multiply adenylylated forms of glutamine synthetase from Escherichia coli: a model relating both concentration and density of antigenic sites with the antibody-antigen interaction.

Hohman, R J, Rhee, S G, Stadtman, E R

Sheep antibodies directed against an AMP-bovine serum albumin conjugate exhibit highly specific binding toward AMP. These antibodies bind to the AMP moiety of adenylylated glutamine synthetase...

The erbB-2 mitogenic signaling pathway: tyrosine phosphorylation of phospholipase C-gamma and GTPase-activating protein does not correlate with erbB-2 mitogenic potency.

Fazioli, F, Kim, U H, Rhee, S G, Molloy, C J, Segatto, O, Di Fiore, P P

The erbB-2 gene product, gp185erbB-2, unlike the structurally related epidermal growth factor (EGF) receptor (EGFR), exhibits constitutive kinase and transforming activity. We used a chimeric...

Phosphorylation of Nck in response to a variety of receptors, phorbol myristate acetate, and cyclic AMP.

Park, D, Rhee, S G

The 47-kDa protein coimmunoprecipitated with phospholipase C (PLC)-gamma 1 by anti-PLC-gamma 1 monoclonal antibodies is proved to be Nck, a protein composed almost exclusively of one SH2 and three...

Platelet-derived growth factor (PDGF)-dependent association of phospholipase C-gamma with the PDGF receptor signaling complex.

Morrison, D K, Kaplan, D R, Rhee, S G, Williams, L T

We investigated the interaction of phospholipase C-gamma (PLC-gamma) with wild-type and mutant forms of the platelet-derived growth factor (PDGF) beta-receptor both in vivo and in vitro. After PDGF...

Platelet-derived growth factor induces rapid and sustained tyrosine phosphorylation of phospholipase C-gamma in quiescent BALB/c 3T3 cells.

Wahl, M I, Olashaw, N E, Nishibe, S, Rhee, S G, Pledger, W J, Carpenter, G

Platelet-derived growth factor (PDGF) stimulates the proliferation of quiescent fibroblasts through a series of events initiated by activation of tyrosine kinase activity of the PDGF receptor at the...

A site of tyrosine phosphorylation in the C terminus of the epidermal growth factor receptor is required to activate phospholipase C.

Vega, Q C, Cochet, C, Filhol, O, Chang, C P, Rhee, S G, Gill, G N

Cells expressing mutant epidermal growth factor (EGF) receptors have been used to study mechanisms through which EGF increases phospholipase C (PLC) activity. C-terminal truncation mutant EGF...

Separation of glutamine synthetase species with different states of adenylylation by chromatography on monoclonal anti-AMP antibody affinity columns.

Chung, H K, Rhee, S G

Glutamine synthetase [GS; L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] from Escherichia coli is composed of 12 identical subunits arranged in two superimposed hexagonal arrays. Therefore,...

Allosteric regulation of monocyclic interconvertible enzyme cascade systems: Use of Escherichia coli glutamine synthetase as an experimental model

Rhee, S. G., Park, R., Chock, P. B., Stadtman, E. R.

The interconversion of Escherichia coli glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] between its adenylylated and unadenylylated forms has been used to verify the...

Topographical analysis of regulatory and metal ion binding sites on glutamine synthetase from Escherichia coli: 13C and 31P nuclear magnetic resonance and fluorescence energy transfer study

Villafranca, J. J., Rhee, S. G., Chock, P. B.

The paramagnetic effect of Mn(II) on 13C and 31P nuclear magnetic resonance signals from the [2-13C]ATP adenylylated glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming); EC 6.3.1.2] from...

The product of a newly identified gene, gInF, is required for synthesis of glutamine synthetase in Salmonella.

Garcia, E, Bancroft, S, Rhee, S G, Kustu, S

The product of a newly identified gene, glnF, which is distinct from the glutamine synthetase structural gene (glnA), is required for synthesis of glutamine synthetase [L-glutamate:ammonia ligase...

Modified kinetics of platelet-derived growth factor-induced Ca2+ increases in NIH-3T3 cells overexpressing phospholipase C gamma 1.

Renard, D C, Bolton, M M, Rhee, S G, Margolis, B L, Zilberstein, A, Schlessinger, J, ...

The effects of platelet-derived growth factor (PDGF) on cytosolic free Ca2+ concentration ([Ca2+]i) and inositol phosphates were studied in NIH-3T3 fibroblasts transfected with cDNA for phospholipase...

Evidence that the epidermal growth factor receptor and non-tyrosine kinase hormone receptors stimulate phosphoinositide hydrolysis by independent pathways.

Hepler, J R, Jeffs, R A, Huckle, W R, Outlaw, H E, Rhee, S G, Earp, H S, ...

We have shown previously that exposure of a non-transformed continuous line of rat liver epithelial (WB) cells to epidermal growth factor (EGF), adrenaline, angiotensin II or [Arg8]vasopressin...

Phosphoinositide signalling enzymes in rat liver nuclei: phosphoinositidase C isoform beta 1 is specifically, but not predominantly, located in the nucleus.

Divecha, N, Rhee, S G, Letcher, A J, Irvine, R F

The presence of phosphoinositide-mobilizing enzymes has been investigated in purified rat liver nuclei by radiolabelling and by probing with antibodies. A Ca(2+)-activated phosphoinositidase C (PIC)...

Changes in the components of a nuclear inositide cycle during differentiation in murine erythroleukaemia cells.

Divecha, N, Letcher, A J, Banfic, H H, Rhee, S G, Irvine, R F

Differentiation of murine erythroleukaemia cells with the chemical agent DMSO leads to a cessation of proliferation and the production of a number of erythrocyte markers such as haemoglobin. We have...

Cyclic AMP agonists induce the phosphorylation of phospholipase C-tau and of a 76 kDa protein co-precipitated by anti-(phospholipase C-tau) monoclonal antibodies in BALB/c-3T3 cells. Relationship to inositol phosphate formation.

Olashaw, N E, Rhee, S G, Pledger, W J

Previous studies have demonstrated enhanced phosphorylation of phospholipase C-tau (PLC-tau), a key regulatory enzyme in phosphoinositide metabolism, in cells treated with platelet-derived growth...

Asymmetric signal transduction in polarized ileal Na(+)-absorbing cells: carbachol activates brush-border but not basolateral-membrane PIP2-PLC and translocates PLC-gamma 1 only to the brush border.

Khurana, S, Kreydiyyeh, S, Aronzon, A, Hoogerwerf, W A, Rhee, S G, Donowitz, M, ...

In ileal Na+ absorptive cells, carbachol inhibits NaCl absorption and its component brush-border Na+/H+ exchanger, acting via basolateral membrane (BLM) receptors. This carbachol effect involves...