Commentary. Who contracts for primary care? (1999)
Lewis, R, Gillam, S, Gosden, T, Sheaff, R
The implications of the 1997 (Primary Care) Act have been largely overlooked in the rush to establish Primary Care Groups. Allowing health authorities to develop local contracts for primary care has...
Hamlyn, P.H., Gillam, S., Smith, M., Milstein, C.
Using an oligonucleotide d(pTl0-C-A) as primer, cDNA has been transcribed from the 3′ non-coding region of mouse immunoglobulin light chain mRNA and sequenced by a modification1 of the...
Liu, Z, Yang, D, Qiu, Z, Lim, K T, Chong, P, Gillam, S
In rubella virus-infected cells, genomic 40S and subgenomic 24S RNAs are present in the cytoplasm of infected cells. However, encapsidation by rubella virus capsid protein is specific for 40S genomic...
Qiu, Z, Ou, D, Hobman, T C, Gillam, S
Rubella virus (RV) virions contain two envelope glycoproteins (E1 and E2) and a capsid protein (C). Noninfectious RV-like particles (VLPs) containing three structural proteins were expressed in a BHK...
Ou, D, Chong, P, Choi, Y, McVeigh, P, Jefferies, W A, Koloitis, G, ...
T-cell epitopes on the E2 protein of rubella virus were studied by using 15 overlapping synthetic peptides covering the E2 protein sequence. The most frequently recognized epitopes on E2 were E2-4...
Yoon, J W, Choi, D S, Liang, H C, Baek, H S, Ko, I Y, Jun, H S, ...
Glycosylated, membrane-associated E1 (58-kDa) and E2 (47- to 49-kDa) rubella virus proteins and unglycosylated nucleoprotein C (33 kDa), from separately expressed vaccinia virus recombinants, were...
Analysis of T- and B-cell epitopes of capsid protein of rubella virus by using synthetic peptides.
Ou, D, Chong, P, Tripet, B, Gillam, S
A nested set of 11 overlapping synthetic peptides covering the entire sequence of rubella virus capsid protein was synthesized, purified, and tested against human rubella virus-specific T-cell lines...
Qiu, Z, Hobman, T C, McDonald, H L, Seto, N O, Gillam, S
The role of N-linked glycosylation in processing and intracellular transport of rubella virus glycoprotein E2 has been studied by expressing glycosylation mutants of E2 in COS cells. A panel of E2...
Translocation of rubella virus glycoprotein E1 into the endoplasmic reticulum.
Hobman, T C, Shukin, R, Gillam, S
Rubella virus (RV) contains four structural proteins, C (capsid), E2a, E2b, and E1, which are derived from posttranslational processing of a single polyprotein precursor, p110. C protein is...
Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.
Gillam, S, Astell, C R, Jahnke, P, Hutchison, C A, Smith, M
Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without...
Gillam, S, Atkinson, T, Markham, A, Smith, M
Site-directed mutagenesis has been used to produce a T----A change at nucleotide 70 of phi X174 genome. This generates an am codon, TAG, in the gene K reading frame without affecting the amino acid,...
Cellular and humoral immune responses to rubella virus structural proteins E1, E2, and C.
Chaye, H H, Mauracher, C A, Tingle, A J, Gillam, S
Better understanding of cell-mediated immune responses to rubella virus would provide the basis for the development of safe and effective vaccines against rubella and would aid in analysis of the...
Gillam, S, Jahnke, P, Astell, C, Phillips, S, Hutchison, C A, Smith, M
The oligodeoxyribonucleotides, pCCCAGCCTCAA, which is complementary to nucleotides 5274--4284 of bacteriophage phi X174 viral DNA , and pCCCAGCCTAAA, which corresponds to the same sequence with a C...
Clarke, D M, Loo, T W, Hui, I, Chong, P, Gillam, S
The complete nucleotide sequence of the 24S subgenomic mRNA of wild-type M33 strain rubella virus has been determined. This RNA is 3,383 nucleotides in length excluding the 3'-terminal poly(A) tract....
Sequence analysis of the 3' non-coding region of mouse immunoglobulin light chain messenger RNA.
Hamlyn, P H, Gillam, S, Smith, M, Milstein, C
Using an oligonucleotide d(pT10-C-A) as primer, cDNA has been transcribed from the 3' non-coding region of mouse immunoglobulin light chain mRNA and sequenced by a modification1 of the 'plus-minus'...
Gillam, S, Waterman, K, Smith, M
Polynucleotide phosphorylase from Escherichia coli can be used to catalyse the addition of short tracts of deoxyadenylate residues to the 3'-termini of deoxyribooligonucleotides of the type pdAn-dN...
The base-pairing specificity of cellulose-pdT9.
Gillam, S, Waterman, K, Smith, M
The stability of the interaction of oligoadenylates, containing single nucleotide substitutions, with cellulose-pdT9 has been studied by thermal elution. In the case of oligodeoxyriboadenylates, the...
Variation in the polyadenylylation site of bovine prolactin mRNA.
Sasavage, N L, Smith, M, Gillam, S, Woychik, R P, Rottman, F M
The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple...
Gillam, S, Rottman, F, Jahnke, P, Smith, M
The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic...
Langham, S, Gillam, S, Thorogood, M
BACKGROUND: Financial incentives for increasing health promotion activity in primary care, introduced with the 1990 contract for general practitioners, were amended in 1993 and are now focused on...
The ethnic minority linkworker: a key member of the primary health care team?
We present an evaluation of the role of a link-worker trained in health promotion and aspects of chronic disease management. A shift in workload occurred from the practice nurse to link-worker, and...
Liu, Z, Yang, D, Qiu, Z, Lim, K T, Chong, P, Gillam, S
In rubella virus-infected cells, genomic 40S and subgenomic 24S RNAs are present in the cytoplasm of infected cells. However, encapsidation by rubella virus capsid protein is specific for 40S genomic...
Qiu, Z, Ou, D, Hobman, T C, Gillam, S
Rubella virus (RV) virions contain two envelope glycoproteins (E1 and E2) and a capsid protein (C). Noninfectious RV-like particles (VLPs) containing three structural proteins were expressed in a BHK...
Ou, D, Chong, P, Choi, Y, McVeigh, P, Jefferies, W A, Koloitis, G, ...
T-cell epitopes on the E2 protein of rubella virus were studied by using 15 overlapping synthetic peptides covering the E2 protein sequence. The most frequently recognized epitopes on E2 were E2-4...
Yoon, J W, Choi, D S, Liang, H C, Baek, H S, Ko, I Y, Jun, H S, ...
Glycosylated, membrane-associated E1 (58-kDa) and E2 (47- to 49-kDa) rubella virus proteins and unglycosylated nucleoprotein C (33 kDa), from separately expressed vaccinia virus recombinants, were...
Analysis of T- and B-cell epitopes of capsid protein of rubella virus by using synthetic peptides.
Ou, D, Chong, P, Tripet, B, Gillam, S
A nested set of 11 overlapping synthetic peptides covering the entire sequence of rubella virus capsid protein was synthesized, purified, and tested against human rubella virus-specific T-cell lines...
Qiu, Z, Hobman, T C, McDonald, H L, Seto, N O, Gillam, S
The role of N-linked glycosylation in processing and intracellular transport of rubella virus glycoprotein E2 has been studied by expressing glycosylation mutants of E2 in COS cells. A panel of E2...
Translocation of rubella virus glycoprotein E1 into the endoplasmic reticulum.
Hobman, T C, Shukin, R, Gillam, S
Rubella virus (RV) contains four structural proteins, C (capsid), E2a, E2b, and E1, which are derived from posttranslational processing of a single polyprotein precursor, p110. C protein is...
Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.
Gillam, S, Astell, C R, Jahnke, P, Hutchison, C A, Smith, M
Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without...
Gillam, S, Atkinson, T, Markham, A, Smith, M
Site-directed mutagenesis has been used to produce a T----A change at nucleotide 70 of phi X174 genome. This generates an am codon, TAG, in the gene K reading frame without affecting the amino acid,...
Cellular and humoral immune responses to rubella virus structural proteins E1, E2, and C.
Chaye, H H, Mauracher, C A, Tingle, A J, Gillam, S
Better understanding of cell-mediated immune responses to rubella virus would provide the basis for the development of safe and effective vaccines against rubella and would aid in analysis of the...
Gillam, S, Jahnke, P, Astell, C, Phillips, S, Hutchison, C A, Smith, M
The oligodeoxyribonucleotides, pCCCAGCCTCAA, which is complementary to nucleotides 5274--4284 of bacteriophage phi X174 viral DNA , and pCCCAGCCTAAA, which corresponds to the same sequence with a C...
Clarke, D M, Loo, T W, Hui, I, Chong, P, Gillam, S
The complete nucleotide sequence of the 24S subgenomic mRNA of wild-type M33 strain rubella virus has been determined. This RNA is 3,383 nucleotides in length excluding the 3'-terminal poly(A) tract....
Sequence analysis of the 3' non-coding region of mouse immunoglobulin light chain messenger RNA.
Hamlyn, P H, Gillam, S, Smith, M, Milstein, C
Using an oligonucleotide d(pT10-C-A) as primer, cDNA has been transcribed from the 3' non-coding region of mouse immunoglobulin light chain mRNA and sequenced by a modification1 of the 'plus-minus'...
Gillam, S, Waterman, K, Smith, M
Polynucleotide phosphorylase from Escherichia coli can be used to catalyse the addition of short tracts of deoxyadenylate residues to the 3'-termini of deoxyribooligonucleotides of the type pdAn-dN...
The base-pairing specificity of cellulose-pdT9.
Gillam, S, Waterman, K, Smith, M
The stability of the interaction of oligoadenylates, containing single nucleotide substitutions, with cellulose-pdT9 has been studied by thermal elution. In the case of oligodeoxyriboadenylates, the...
Variation in the polyadenylylation site of bovine prolactin mRNA.
Sasavage, N L, Smith, M, Gillam, S, Woychik, R P, Rottman, F M
The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple...
Gillam, S, Rottman, F, Jahnke, P, Smith, M
The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic...
Langham, S, Gillam, S, Thorogood, M
BACKGROUND: Financial incentives for increasing health promotion activity in primary care, introduced with the 1990 contract for general practitioners, were amended in 1993 and are now focused on...
The ethnic minority linkworker: a key member of the primary health care team?
We present an evaluation of the role of a link-worker trained in health promotion and aspects of chronic disease management. A shift in workload occurred from the practice nurse to link-worker, and...
Cooperation or conflict over child health surveillance? Views of key actors.
Gillam, S, Glickman, M, Boyle, G, Woodroffe, C
OBJECTIVE--To describe the views of general practitioners, health visitors, and clinical medical officers on child health surveillance, recent changes, perceptions of each other's roles, and...
What makes general practitioners do child health surveillance?
Glickman, M, Gillam, S, Boyle, G, Woodroffe, C
The contribution of different general practitioner characteristics, views, and experiences to the likelihood of their providing child health surveillance (CHS) was determined and their perceived...