TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Wray, L V, Ferson, A E, Rohrer, K, Fisher, S H
Expression of the Bacillus subtilis nrgAB operon is derepressed during nitrogen-limited growth. We have identified a gene, tnrA, that is required for the activation of nrgAB expression under these...
The Bacillus subtilis ureABC operon.
Cruz-Ramos, H, Glaser, P, Wray, L V, Fisher, S H
The Bacillus subtilis ureABC operon encodes homologs of the three subunits of urease enzymes of the family Enterobacteriaceae. Disruption of ureC prevented utilization of urea as a nitrogen source...
Wray, L V, Ferson, A E, Fisher, S H
Expression of urease, which is encoded by the ureABC operon, is regulated in response to nitrogen availability in Bacillus subtilis. Three ureABC promoters were identified in primer extension...
Expression of the Bacillus subtilis hut operon is induced by histidine and subject to regulation by carbon catabolite repression and amino acid repression. A set of hut-lacZ transcriptional fusions...
Atkinson, M R, Wray, L V, Fisher, S H
During growth of Bacillus subtilis in nutrient sporulation medium containing histidine (DSM-His medium), the expression of histidase, the first enzyme in the histidine-degradative pathway (hut), is...
Wray, L V, Atkinson, M R, Fisher, S H
Expression of beta-galactosidase encoded by the nrg-29::Tn917-lacZ insertion increases 4,000-fold during nitrogen-limited growth (M.R. Atkinson and S. H. Fisher, J. Bacteriol. 173:23-27, 1991). The...
Wray, L V, Pettengill, F K, Fisher, S H
Expression of the Bacillus subtilis hut operon is subject to regulation by catabolite repression. A set of hut-lacZ transcriptional fusions was constructed and used to identify two cis-acting sites...
Modulation of Bacillus subtilis catabolite repression by transition state regulatory protein AbrB.
Fisher, S H, Strauch, M A, Atkinson, M R, Wray, L V
The first enzyme of the Bacillus subtilis histidine-degradative (hut) pathway, histidase, was expressed at higher levels during the onset of the stationary growth phase in nutrient sporulation medium...
The levels of urease and asparaginase were elevated 25- and 20-fold, respectively, in extracts of Bacillus subtilis cells grown in medium containing nitrogen sources that are poor sources of ammonium...
Wray, L V, Atkinson, M R, Fisher, S H
Six Streptomyces coelicolor mutants that required glutamine for growth at the wild-type rate on all nitrogen sources (Gln-) were isolated. The phenotypes of all six mutants were similar. The...
Glutamate synthesis in Streptomyces coelicolor.
Both glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) are involved in glutamate synthesis in Streptomyces coelicolor. The highest levels of GDH were seen in extracts of cells grown with...
Regulation of glutamine synthetase in Streptomyces coelicolor.
Glutamine synthetase (GS) in Streptomyces coelicolor was shown to be regulated at two levels. First, the S. coelicolor GS protein is subject to a posttranslational covalent modification which is...
Atkinson, M R, Wray, L V, Fisher, S H
The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing...
Strain SF22, a glutamine-requiring (Gln-) mutant of Bacillus subtilis SMY, is likely to have a mutation in the structural gene for glutamine synthetase, since this strain synthesized 22 to 55% as...
Isolation of Bacillus subtilis mutants pleiotropically insensitive to glucose catabolite repression.
A pleiotropic mutant of Bacillus subtilis was isolated which overproduced in the presence of glucose several enzymes whose synthesis is subject to glucose catabolite repression. Examination of...
Bacillus subtilis mutants deficient in the 2-ketoglutarate dehydrogenase enzymatic complex required aspartate for growth at wild-type rates on carbon sources for which synthesis of the degradative...
2-Ketoglutarate and the regulation of aconitase and histidase formation in Bacillus subtilis.
In contrast to wild-type cells, the Bacillus subtilis mutant SF109 that lacks the active 2-ketoglutarate dehydrogenase enzymatic complex is unable to increase the specific activity of two enzymes...
Lipiarmycin-resistant ribonucleic acid polymerase mutants of Bacillus subtilis.
Sonenshein, A L, Alexander, H B, Rothstein, D M, Fisher, S H
Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of...
Role of CodY in regulation of the Bacillus subtilis hut operon.
Fisher, S H, Rohrer, K, Ferson, A E
Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated...
Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product.
Schreier, H J, Fisher, S H, Sonenshein, A L
Expression of the cloned glnA gene [coding for glutamine synthetase (EC 6.3.1.2)] of Bacillus subtilis was 10-fold higher in an Escherichia coli strain grown under nitrogen-limiting conditions than...
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Wray, L V, Ferson, A E, Rohrer, K, Fisher, S H
Expression of the Bacillus subtilis nrgAB operon is derepressed during nitrogen-limited growth. We have identified a gene, tnrA, that is required for the activation of nrgAB expression under these...
The Bacillus subtilis ureABC operon.
Cruz-Ramos, H, Glaser, P, Wray, L V, Fisher, S H
The Bacillus subtilis ureABC operon encodes homologs of the three subunits of urease enzymes of the family Enterobacteriaceae. Disruption of ureC prevented utilization of urea as a nitrogen source...
Wray, L V, Ferson, A E, Fisher, S H
Expression of urease, which is encoded by the ureABC operon, is regulated in response to nitrogen availability in Bacillus subtilis. Three ureABC promoters were identified in primer extension...
Expression of the Bacillus subtilis hut operon is induced by histidine and subject to regulation by carbon catabolite repression and amino acid repression. A set of hut-lacZ transcriptional fusions...
Atkinson, M R, Wray, L V, Fisher, S H
During growth of Bacillus subtilis in nutrient sporulation medium containing histidine (DSM-His medium), the expression of histidase, the first enzyme in the histidine-degradative pathway (hut), is...
Wray, L V, Atkinson, M R, Fisher, S H
Expression of beta-galactosidase encoded by the nrg-29::Tn917-lacZ insertion increases 4,000-fold during nitrogen-limited growth (M.R. Atkinson and S. H. Fisher, J. Bacteriol. 173:23-27, 1991). The...
Wray, L V, Pettengill, F K, Fisher, S H
Expression of the Bacillus subtilis hut operon is subject to regulation by catabolite repression. A set of hut-lacZ transcriptional fusions was constructed and used to identify two cis-acting sites...
Modulation of Bacillus subtilis catabolite repression by transition state regulatory protein AbrB.
Fisher, S H, Strauch, M A, Atkinson, M R, Wray, L V
The first enzyme of the Bacillus subtilis histidine-degradative (hut) pathway, histidase, was expressed at higher levels during the onset of the stationary growth phase in nutrient sporulation medium...
The levels of urease and asparaginase were elevated 25- and 20-fold, respectively, in extracts of Bacillus subtilis cells grown in medium containing nitrogen sources that are poor sources of ammonium...
Wray, L V, Atkinson, M R, Fisher, S H
Six Streptomyces coelicolor mutants that required glutamine for growth at the wild-type rate on all nitrogen sources (Gln-) were isolated. The phenotypes of all six mutants were similar. The...
Glutamate synthesis in Streptomyces coelicolor.
Both glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) are involved in glutamate synthesis in Streptomyces coelicolor. The highest levels of GDH were seen in extracts of cells grown with...
Regulation of glutamine synthetase in Streptomyces coelicolor.
Glutamine synthetase (GS) in Streptomyces coelicolor was shown to be regulated at two levels. First, the S. coelicolor GS protein is subject to a posttranslational covalent modification which is...
Atkinson, M R, Wray, L V, Fisher, S H
The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing...
Strain SF22, a glutamine-requiring (Gln-) mutant of Bacillus subtilis SMY, is likely to have a mutation in the structural gene for glutamine synthetase, since this strain synthesized 22 to 55% as...
Isolation of Bacillus subtilis mutants pleiotropically insensitive to glucose catabolite repression.
A pleiotropic mutant of Bacillus subtilis was isolated which overproduced in the presence of glucose several enzymes whose synthesis is subject to glucose catabolite repression. Examination of...
Bacillus subtilis mutants deficient in the 2-ketoglutarate dehydrogenase enzymatic complex required aspartate for growth at wild-type rates on carbon sources for which synthesis of the degradative...
2-Ketoglutarate and the regulation of aconitase and histidase formation in Bacillus subtilis.
In contrast to wild-type cells, the Bacillus subtilis mutant SF109 that lacks the active 2-ketoglutarate dehydrogenase enzymatic complex is unable to increase the specific activity of two enzymes...
Lipiarmycin-resistant ribonucleic acid polymerase mutants of Bacillus subtilis.
Sonenshein, A L, Alexander, H B, Rothstein, D M, Fisher, S H
Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of...
Role of CodY in regulation of the Bacillus subtilis hut operon.
Fisher, S H, Rohrer, K, Ferson, A E
Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated...
Regulation of expression from the glnA promoter of Bacillus subtilis requires the glnA gene product.
Schreier, H J, Fisher, S H, Sonenshein, A L
Expression of the cloned glnA gene [coding for glutamine synthetase (EC 6.3.1.2)] of Bacillus subtilis was 10-fold higher in an Escherichia coli strain grown under nitrogen-limiting conditions than...