Handwerger, S, Skoble, J, Discotto, L F, Pucci, M J
In several strains of Enterococcus faecium isolated in Europe, the cluster of genes encoding high-level resistance to vancomycin (VanA phenotype) resides on a 10.85-kb transposon, Tn1546, or closely...
Sahm, D F, Free, L, Handwerger, S
Clinical isolates Enterococcus gallinarum AIB39 and E. gallinarum GS1 were studied to establish whether the expression of vanC-1-mediated resistance may be inducible or constitutive. By growth curve...
A clinical isolate of Enterococcus faecium that contains a chromosomally encoded vanA gene cluster, Tn1546::IS1251, transferred vancomycin resistance to the plasmid-free strain Enterococcus faecalis...
Altered peptidoglycan composition in vancomycin-resistant Enterococcus faecalis.
De Jonge, B L, Handwerger, S, Gage, D
The muropeptide compositions of isogenic vancomycin-resistant and -susceptible Enterococcus faecalis strains were analyzed by reverse-phase high-performance liquid chromatography combined with amino...
Vancomycin resistance is encoded on a pheromone response plasmid in Enterococcus faecium 228.
Handwerger, S, Pucci, M J, Kolokathis, A
In Enterococcus faecium 228, vancomycin resistance is encoded on a 55-kilobase conjugative plasmid, pHKK100. This plasmid was transferred with high frequency into susceptible strains of Enterococcus...
Earlier studies have shown that the highly penicillin-resistant South African Strains of pneumococci contain altered penicillin-binding proteins (PBPs) (S. Zighelboim and A. Tomasz, Antimicrob....
Hakenbeck, R, Ellerbrok, H, Briese, T, Handwerger, S, Tomasz, A
There are several major differences between the penicillin-binding proteins (PBPs) of highly penicillin-resistant and -susceptible strains of pneumococci. The highest-molecular-size PBP 1a (98...
Shonekan, D, Mildvan, D, Handwerger, S
The in vitro activities of teicoplanin, daptomycin, ramoplanin, and PD127,391, a new quinolone, were compared with that of vancomycin. Teicoplanin showed the lowest MICs against Enterococcus...
Handwerger, S, Pucci, M J, Volk, K J, Liu, J, Lee, M S
The emergence of acquired high-level resistance among Enterococcus species has renewed interest in mechanisms of resistance to glycopeptide antibiotics in gram-positive bacteria. In Enterococcus...
Handwerger, S, Pucci, M J, Volk, K J, Liu, J, Lee, M S
Vancomycin resistance plasmids in enterococci carry the genes vanH and vanA, which encode enzymes catalyzing, respectively, the reduction of 2-keto acids to 2-D-hydroxy acids and the addition of...
Vancomycin-dependent Enterococcus faecium isolated from stool following oral vancomycin therapy.
Dever, L L, Smith, S M, Handwerger, S, Eng, R H
The isolation of clinical strains of enterococci requiring vancomycin for growth has only recently been reported. We describe the isolation of Enterococcus faecium requiring vancomycin for growth...
Derivatives of the highly vancomycin-resistant Enterococcus faecalis strain 221 (MIC, 1,024 micrograms/ml) harboring Tn917 insertions in vanR, vanH, and vanA were compared with the parent strain and...
Thraikill, K M, Clemmons, D R, Busby, W H, Handwerger, S
Several growth hormone-independent 25-31,000 kD insulin-like growth factor binding proteins (IGF-BPs) have been identified in plasma, extravascular fluids, and various cell-conditioned media....
Handwerger, S, Quarfordt, S, Barrett, J, Harman, I
High density lipoproteins (HDL) stimulated a dose-dependent increase in the release of placental lactogen (hPL) from human placental explants. The stimulation was not prevented by delipidation of HDL...
Localization and specificity of binding of subprimate placental lactogen in rabbit tissues.
Bolander, F F, Hurley, T W, Handwerger, S, Fellows, R E
A search for specific placental lactogen binding was undertaken in tissues obtained from late pregnant rabbits using the placental lactogens from sheep, cows, and human beings. 125I-labeled ovine...
Vasopressin, angiotensin and phenylephrine stimulate glycogenolysis in postnatal rat liver by a Ca2+-mediated mechanism not involving cyclic AMP. To determine whether these hormones promote...
Handwerger, S, Skoble, J, Discotto, L F, Pucci, M J
In several strains of Enterococcus faecium isolated in Europe, the cluster of genes encoding high-level resistance to vancomycin (VanA phenotype) resides on a 10.85-kb transposon, Tn1546, or closely...
Sahm, D F, Free, L, Handwerger, S
Clinical isolates Enterococcus gallinarum AIB39 and E. gallinarum GS1 were studied to establish whether the expression of vanC-1-mediated resistance may be inducible or constitutive. By growth curve...
A clinical isolate of Enterococcus faecium that contains a chromosomally encoded vanA gene cluster, Tn1546::IS1251, transferred vancomycin resistance to the plasmid-free strain Enterococcus faecalis...
Altered peptidoglycan composition in vancomycin-resistant Enterococcus faecalis.
De Jonge, B L, Handwerger, S, Gage, D
The muropeptide compositions of isogenic vancomycin-resistant and -susceptible Enterococcus faecalis strains were analyzed by reverse-phase high-performance liquid chromatography combined with amino...
Vancomycin resistance is encoded on a pheromone response plasmid in Enterococcus faecium 228.
Handwerger, S, Pucci, M J, Kolokathis, A
In Enterococcus faecium 228, vancomycin resistance is encoded on a 55-kilobase conjugative plasmid, pHKK100. This plasmid was transferred with high frequency into susceptible strains of Enterococcus...
Earlier studies have shown that the highly penicillin-resistant South African Strains of pneumococci contain altered penicillin-binding proteins (PBPs) (S. Zighelboim and A. Tomasz, Antimicrob....
Hakenbeck, R, Ellerbrok, H, Briese, T, Handwerger, S, Tomasz, A
There are several major differences between the penicillin-binding proteins (PBPs) of highly penicillin-resistant and -susceptible strains of pneumococci. The highest-molecular-size PBP 1a (98...
Shonekan, D, Mildvan, D, Handwerger, S
The in vitro activities of teicoplanin, daptomycin, ramoplanin, and PD127,391, a new quinolone, were compared with that of vancomycin. Teicoplanin showed the lowest MICs against Enterococcus...
Handwerger, S, Pucci, M J, Volk, K J, Liu, J, Lee, M S
The emergence of acquired high-level resistance among Enterococcus species has renewed interest in mechanisms of resistance to glycopeptide antibiotics in gram-positive bacteria. In Enterococcus...
Handwerger, S, Pucci, M J, Volk, K J, Liu, J, Lee, M S
Vancomycin resistance plasmids in enterococci carry the genes vanH and vanA, which encode enzymes catalyzing, respectively, the reduction of 2-keto acids to 2-D-hydroxy acids and the addition of...
Vancomycin-dependent Enterococcus faecium isolated from stool following oral vancomycin therapy.
Dever, L L, Smith, S M, Handwerger, S, Eng, R H
The isolation of clinical strains of enterococci requiring vancomycin for growth has only recently been reported. We describe the isolation of Enterococcus faecium requiring vancomycin for growth...
Derivatives of the highly vancomycin-resistant Enterococcus faecalis strain 221 (MIC, 1,024 micrograms/ml) harboring Tn917 insertions in vanR, vanH, and vanA were compared with the parent strain and...
Thraikill, K M, Clemmons, D R, Busby, W H, Handwerger, S
Several growth hormone-independent 25-31,000 kD insulin-like growth factor binding proteins (IGF-BPs) have been identified in plasma, extravascular fluids, and various cell-conditioned media....
Handwerger, S, Quarfordt, S, Barrett, J, Harman, I
High density lipoproteins (HDL) stimulated a dose-dependent increase in the release of placental lactogen (hPL) from human placental explants. The stimulation was not prevented by delipidation of HDL...
Localization and specificity of binding of subprimate placental lactogen in rabbit tissues.
Bolander, F F, Hurley, T W, Handwerger, S, Fellows, R E
A search for specific placental lactogen binding was undertaken in tissues obtained from late pregnant rabbits using the placental lactogens from sheep, cows, and human beings. 125I-labeled ovine...
Vasopressin, angiotensin and phenylephrine stimulate glycogenolysis in postnatal rat liver by a Ca2+-mediated mechanism not involving cyclic AMP. To determine whether these hormones promote...
Orellana, J, Teich, S A, Winterkorn, J S, Mathur-Wagh, U, Handwerger, S, Schlamm, H, ...
Six patients (11 eyes) with virologically confirmed cytomegalovirus (CMV) retinitis involving the posterior pole of the eye were treated with a new drug, ganciclovir. Treatment with intravenous...