S. Hayashi

Immunoglobulin G1 Antibody Response to Helicobacter pylori Heat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

Aging and disease processes may be due to deleterious and irreversible changes produced by free radical reactions. The enzyme copper-zinc superoxide dismutase (Cu-Zn SOD; superoxide:superoxide...

Ishii, E., Yokota, K., Sugiyama, T., Fujinaga, Y., Ayada, K., Hokari, I., ...

Gastric mucosa-associated lymphoid tissue (MALT) lymphoma is related to Helicobacter pylori infection. Specifically, it has been pointed out that pathogenesis of MALT lymphoma involves the 60-kDa...

Functional modulation of estrogen receptor by redox state with reference to thioredoxin as a mediator.

Hayashi, S, Hajiro-Nakanishi, K, Makino, Y, Eguchi, H, Yodoi, J, Tanaka, H

Redox regulation of transcription factors has recently been demonstrated for AP-1, NF-kappaB, Sp-1 and glucocorticoid receptor in vitro and in vivo. The redox state in estrogen-dependent cells...

Regulation of estrogen receptor alpha gene mediated by promoter B responsible for its enhanced expressionin human breast cancer.

Tanimoto, K, Eguchi, H, Yoshida, T, Hajiro-Nakanishi, K, Hayashi, S

We have previously reported that transcription from a distal promoter (promoter B) of the estrogen receptor alpha (ERalpha) gene is responsible for the increased expression of ERalpha in human breast...

Reactivities of Lewis antigen monoclonal antibodies with the lipopolysaccharides of Helicobacter pylori strains isolated from patients with gastroduodenal diseases in Japan.

Amano, K, Hayashi, S, Kubota, T, Fujii, N, Yokota, S

We have examined the reactivity of monoclonal antibodies (MAbs) specific for Lewis antigens (Le(x), Le(y), Le(a), and Le(b)) with Helicobacter pylori lipopolysaccharides (LPS) by immunoblot analysis...

In vitro effects of beta-lactams combined with beta-lactamase inhibitors against methicillin-resistant Staphylococcus aureus.

In vitro inhibition of the infectivity and replication of human immunodeficiency virus type 1 by combination of antiretroviral 2',3'-dideoxynucleosides and virus-binding inhibitors.

The effects of combinations of beta-lactams with two beta-lactamase inhibitors, sulbactam and clavulanic acid, were determined in vitro against 22 clinical isolates of methicillin-resistant...

Hayashi, S, Fine, R L, Chou, T C, Currens, M J, Broder, S, Mitsuya, H

We tested the in vitro inhibitory activities of three 2',3'-dideoxynucleosides and two inhibitors of viral binding in combinations against the infectivity and cytopathic effect of human...

Cyclobut-A and cyclobut-G, carbocyclic oxetanocin analogs that inhibit the replication of human immunodeficiency virus in T cells and monocytes and macrophages in vitro.

Hayashi, S, Norbeck, D W, Rosenbrook, W, Fine, R L, Matsukura, M, Plattner, J J, ...

Two newly synthesized carbocyclic oxetanocin analogs, (+/-)-9-[(1 beta,2 alpha,3 beta)-2,3-bis(hydroxymethyl)-1-cyclobutyl]adenine (cyclobut-A) and (+/-)-9-[(1 beta,2 alpha,3...

Simple assay of beta-lactamase with agar medium containing a chromogenic cephalosporin, pyridinium-2-azo-p-dimethylaniline chromophore (PADAC).

Low antigenicity of the polysaccharide region of Helicobacter pylori lipopolysaccharides derived from tumors of patients with gastric cancer.

A new beta-lactamase assay method with agar plates containing pyridinium-2-azo-p-dimethylaniline chromophore (PADAC) (50 microM), a beta-lactamase-labile, chromogenic cephalosporin, was examined. On...

Yokota, S, Amano, K, Hayashi, S, Fujii, N

We have examined the antibody response to Helicobacter pylori lipopolysaccharide (LPS) during natural infection in humans. The sera of over 70% of H. pylori-infected individuals were found to contain...

Distribution of cefpirome (HR 810) to exudate in the croton oil-induced rat granuloma pouch and its therapeutic effects on experimental infections in the pouch.

Arai, S, Kobayashi, S, Hayashi, S, Sakaguchi, T

Therapeutic effects of intravenously administered cefpirome on experimental bacterial infections in croton oil-induced rat granuloma pouches were compared with those of ceftazidime, moxalactam,...

Beta-lactamase stability of cefpirome (HR 810), a new cephalosporin with a broad antimicrobial spectrum.

Kobayashi, S, Arai, S, Hayashi, S, Fujimoto, K

Cefpirome was highly stable to hydrolysis by various beta-lactamases, although it was hydrolyzed to some extent by R plasmid-mediated penicillinase of Richmond-Sykes type Va/b and by chromosomal...

Preparation and regeneration of bacteriophage PL-1 enzyme-induced Lactobacillus casei protoplasts.

Watanabe, K, Hayashida, M, Nakashima, Y, Hayashi, S

Lactobacillus casei ATCC 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the PL-1 phage lysates of this bacterium, at 37 degrees C for 1.5 h in 50 mM...

Synthesis and export of the outer membrane lipoprotein in Escherichia coli mutants defective in generalized protein export.

Watanabe, T, Hayashi, S, Wu, H C

Export of the outer membrane lipoprotein in Escherichia coli was examined in conditionally lethal mutants that were defective in protein export in general, including secA, secB, secC, and secD....

Effect of a mutation preventing lipid modification on localization of the pCloDF13-encoded bacteriocin release protein and on release of cloacin DF13.

Luirink, J, Hayashi, S, Wu, H C, Kater, M M, De Graaf, F K, Oudega, B

The pCloDF13-encoded bacteriocin release protein (BRP; Mr 2,871) is essential for the translocation of cloacin DF13 across the cell envelope of producing Escherichia coli cells. Overproduction of...

Lipid modification of Escherichia coli penicillin-binding protein 3.

Hayashi, S, Hara, H, Suzuki, H, Hirota, Y

The primary structure of penicillin-binding protein 3 (PBP 3), an essential enzyme for cell division in Escherichia coli, was deduced from the nucleotide sequence of the ftsI gene (M. Nakamura, I. N....

Processing of Bacillus licheniformis penicillinases lacking a lipoprotein modification site in Escherichia coli.

Hayashi, S, Chang, S Y, Chang, S, Wu, H C

We have previously shown that the penP Ser-27 prepenicillinase is processed into two forms, Ser-35-penicillinase and Asn-29 penicillinase. Two new penicillinase mutants, penP Ser-27 Pro-28 and penP...

Accumulation of prolipoprotein in Escherichia coli mutants defective in protein secretion.

Globomycin sensitivity of Escherichia coli and Salmonella typhimurium: effects of mutations affecting structures of murein lipoprotein.

The export of lipoprotein has been found to be affected in both secA and secY mutants of Escherichia coli which are defective in the secretion of a number of outer membrane and periplasmic proteins....

Lai, J S, Philbrick, W M, Hayashi, S, Inukai, M, Arai, M, Hirota, Y, ...

The sensitivity of strains of Escherichia coli and Salmonella typhimurium to globomycin is increased in mutants defective in the lipopolysaccharide structure. E. coli mutants altered in the...

Temperature-sensitive mutations in Drosophila melanogaster,I. Relative frequencies among gamma-ray and chemically induced sex-linked recessive lethals and semilethals.

Suzuki, D T, Piternick, L K, Hayashi, S, Tarasoff, M, Baillie, D, Erasmus, U

Structural rearrangement of integrated hepatitis B virus DNA as well as cellular flanking DNA is present in chronically infected hepatic tissues.

Takada, S, Gotoh, Y, Hayashi, S, Yoshida, M, Koike, K

Cellular DNAs from human livers chronically infected with hepatitis B virus (HBV) were analyzed by Southern blot hybridization for the presence of integrated HBV DNA. In 15 of 16 chronically infected...

Isolation and characteristics of collagenolytic enzyme produced by Candida albicans.

Kaminishi, H, Hagihara, Y, Hayashi, S, Cho, T

In media containing collagen as the nitrogen source, the pathogenic yeast Candida albicans secreted a collagenolytic enzyme. Purification of the enzyme from a culture filtrate was achieved by...

Augmented production of colony-stimulating factor in C3H/HeN mice immunized with Nocardia rubra cell wall skeleton.

Hayashi, S, Masuno, T, Hosoe, S, Kawase, I, Sakatani, M, Ogura, T, ...

C3H/HeN mice subcutaneously injected repeatedly with Nocardia rubra cell wall skeleton (N-CWS) acquired cellular immunity against N-CWS. In these N-CWS-immunized mice, the serum colony-stimulating...

Prevalence of antibodies to Coxiella burnetii in Japan.

Htwe, K K, Yoshida, T, Hayashi, S, Miyake, T, Amano, K, Morita, C, ...

We evaluated the prevalence of Coxiella burnetii antibodies in 626 human serum samples (275 from veterinarians, 107 from meat-processing workers, 184 from respiratory-disorder patients, and 60 from...

Adenallene and cytallene: acyclic-nucleoside analogues that inhibit replication and cytopathic effect of human immunodeficiency virus in vitro.

Hayashi, S, Phadtare, S, Zemlicka, J, Matsukura, M, Mitsuya, H, Broder, S

Although several antiretroviral compounds are already known, almost no acyclic nucleoside derivatives lacking an oxacyclopentane have been reported to exert significant inhibition against human...

Drosophila Cu-Zn superoxide dismutase cDNA sequence.

Growth Stasis by Accumulated l-α-Glycerophosphate in Escherichia coli

Cozzarelli, N. R., Koch, J. P., Hayashi, S., Lin, E. C. C.

Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), J. P. Koch, S. Hayashi, and E. C. C. Lin. Growth stasis by accumulated l-α-glycerophosphate in Escherichia coli. J. Bacteriol....

Genes for tRNALys5 from Drosophila melanogaster.

DeFranco, D, Burke, K B, Hayashi, S, Tener, G M, Miller, R C, Söll, D

The sequences of two cloned genes from Drosophila which hybridize with tRNALys5 are reported. One gene, in plasmid pDt39, has a sequence which corresponds to the sequence of tRNA. The other gene, in...

Nucleotide sequence of a pseudogene for rat ornithine decarboxylase.

Kanamoto, R, Yoshimura, M, Hayashi, S, Oka, T

Exon skipping by mutation of an authentic splice site of c-kit gene in W/W mouse.

Hayashi, S, Kunisada, T, Ogawa, M, Yamaguchi, K, Nishikawa, S

The murine mutation dominant white spotting (W) is in the proto-oncogene, c-kit. The receptor tyrosine kinase encoded by this gene has pleiotropic effects on murine development including hemopoietic...

The sequence of the Cu-Zn superoxide dismutase gene of Drosophila.

In vivo competition of delta-crystallin gene expression by DNA fragments containing a GC box.

Hayashi, S, Kondoh, H

Expression of the chicken delta-crystallin gene 1 injected into the nuclei of mouse cells is lens specific. Coinjection of GC box-containing DNA fragments from delta-crystallin, simian virus 40...

In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development.

Nishikawa, S, Kusakabe, M, Yoshinaga, K, Ogawa, M, Hayashi, S, Kunisada, T, ...

Previous studies on mice bearing various mutations within the c-kit gene, dominant white spotting (W), indicate the functional role of this tyrosine kinase receptor in the development of melanocytes,...

A synthetic peptide inhibitor for alpha-chemokines inhibits the growth of melanoma cell lines.

Hayashi, S, Kurdowska, A, Cohen, A B, Stevens, M D, Fujisawa, N, Miller, E J

Melanoma growth stimulatory activity (MGSA/GROalpha) is a 73 amino acid peptide sharing sequence characteristics with the alpha-chemokine superfamily. MGSA/GROalpha is produced by diverse melanoma...

Tissue-specific regulation of a chicken delta-crystallin gene in mouse cells: involvement of the 5' end region.

Hayashi, S, Kondoh, H, Yasuda, K, Soma, G, Ikawa, Y, Okada, T S

A cloned delta-crystallin gene of the chicken is preferentially expressed in lens cells after introduction into various mouse tissues. The level of expression in the lens epithelium is 20 times...

Antizyme, a protein induced by polyamines, accelerates the degradation of ornithine decarboxylase in Chinese-hamster ovary-cell extracts.

Murakami, Y, Tanaka, K, Matsufuji, S, Miyazaki, Y, Hayashi, S

Ornithine decarboxylase (ODC), the key regulatory enzyme for polyamine biosynthesis, is known to have a short intracellular half-life, and antizyme, an ODC-binding protein induced by polyamines, has...

Multiple regulation of ornithine decarboxylase in enzyme-overproducing cells.

Kameji, T, Hayashi, S, Hoshino, K, Kakinuma, Y, Igarashi, K

We have isolated from mouse FM3A cells a variant cell line, termed EXOD-1, that overproduces ornithine decarboxylase (ODC). The cells were resistant to alpha-difluoromethylornithine, an irreversible...

Involvement of the proteasome and antizyme in ornithine decarboxylase degradation by a reticulocyte lysate.

Murakami, Y, Matsufuji, S, Tanaka, K, Ichihara, A, Hayashi, S

Ornithine decarboxylase (ODC) degradation in a freshly prepared reticulocyte lysate was examined. Immunodepletion of proteasomes from the reticulocyte lysate resulted in almost complete loss of ODC...

Ornithine decarboxylase antizyme in kidneys of male and female mice.

Murakami, Y, Marumo, M, Hayashi, S

Antizyme, a protein inhibitor of ornithine decarboxylase (ODC), was shown to be induced in mouse kidney by repeated injection of putrescine. Antizyme was also present as a complex with ODC in the...

Rapid and regulated degradation of ornithine decarboxylase.

Hayashi, S, Murakami, Y

Forced expression of antizyme abolishes ornithine decarboxylase activity, suppresses cellular levels of polyamines and inhibits cell growth.

Murakami, Y, Matsufuji, S, Miyazaki, Y, Hayashi, S

Ornithine decarboxylase (ODC) is a key enzyme in polyamine biosynthesis. It is a short-lived protein and negatively regulated by its products, polyamines. Its degradation is accelerated by the...

Properties and fluctuations in vivo of rat liver antizyme inhibitor.

Murakami, Y, Matsufuji, S, Nishiyama, M, Hayashi, S

Antizyme inhibitor was highly purified from rat liver by using affinity chromatography. It has some structural resemblance to ornithine decarboxylase (ODC), as judged from Mr, immunoreactivity and...

Accumulation of ornithine decarboxylase-antizyme complex in HMOA cells.

Murakami, Y, Fujita, K, Kameji, T, Hayashi, S

A new method was developed for the assay of ornithine decarboxylase (ODC)-antizyme complex, in which alpha-difluoromethylornithine (DFMO)-inactivated ODC was used to release active ODC competitively...

Role of antizyme in degradation of ornithine decarboxylase in HTC cells.

Murakami, Y, Hayashi, S

A good correlation was observed between the reciprocal of the half-life of ornithine decarboxylase (ODC) activity in the presence of cycloheximide and the relative amount of ODC-antizyme complex to...

Identical catalytic-centre activity for mouse kidney and rat liver ornithine decarboxylases as determined with antizyme and affinity labelling.

Marumo, M, Matsufuji, S, Murakami, Y, Hayashi, S

Since the catalytic-centre activity of mouse kidney ornithine decarboxylase (ODC) has been assumed to be twice as high as that of rat liver ODC, we compared relative catalytic-centre activity of the...

Changes in ornithine decarboxylase and antizyme activities in developing mouse brain.

Onoue, H, Matsufuji, S, Nishiyama, M, Murakami, Y, Hayashi, S

A macromolecular inhibitor to ornithine decarboxylase (ODC) present in mouse brain was identified as ODC antizyme [Fong, Heller & Canellakis (1976) Biochim. Biophys. Acta 428, 456-465; Heller, Fong &...

Cysteine-dependent inactivation of hepatic ornithine decarboxylase.

Murakami, Y, Kameji, T, Hayashi, S

When rat liver homogenate or its postmitochondrial supernatant was incubated with L-cysteine, but not D-cysteine, ornithine decarboxylase (ODC) lost more than half of its catalytic activity within 30...

Antizyme to ornithine decarboxylase is present in the liver of starved rats.

Fujita, K, Matsufuji, S, Murakami, Y, Hayashi, S

Antizyme to ornithine decarboxylase (ODC) and ODC-antizyme complex were both present in liver cytosols of starved rats. The antizyme was identified by its molecular weight, kinetic properties,...

A macromolecular inhibitor of the antizyme to ornithine decarboxylase.

Fujita, K, Murakami, Y, Hayashi, S

A macromolecular factor that inhibits the activity of the antizyme to ornithine decarboxylase (ODC) was found in rat liver extracts. The factor, 'antizyme inhibitor', was heat-labile, non diffusable...

Plant leaf alanine: 2-oxoglutarate aminotransferase. Peroxisomal localization and identity with glutamate:glyoxylate aminotransferase.

Noguchi, T, Hayashi, S

The distribution of alanine:2-oxoglutarate aminotransferase (EC 2.6.1.2) in spinach (Spinacia oleracea) leaf homogenates was examined by centrifugation in a sucrose density gradient. About 55% of the...

Translocation of ornithine decarboxylase to the surface membrane during cell activation and transformation.

Heiskala, M, Zhang, J, Hayashi, S, Hölttä, E, Andersson, L C

Ornithine decarboxylase (ODC) is highly up-regulated in proliferating and transforming cells. Here we show that upon induction, an initial cytosolic increase of ODC is followed by translocation of a...

Proteasome pathway operates for the degradation of ornithine decarboxylase in intact cells.

Murakami, Y, Tanahashi, N, Tanaka, K, Omura, S, Hayashi, S

Ornithine decarboxylase (ODC) is degraded in an ATP-dependent manner in vitro by the 26 S proteasome in the presence of antizyme, an ODC destabilizing protein induced by polyamines. In the present...

Two zebrafish (Danio rerio) antizymes with different expression and activities.

Saito, T, Hascilowicz, T, Ohkido, I, Kikuchi, Y, Okamoto, H, Hayashi, S, ...

Cellular polyamines are regulated by a unique feedback mechanism involving ornithine decarboxylase (ODC) antizyme. The synthesis of mammalian antizyme requires a programmed translational frameshift...

Regulation of mammalian phospholipase D2: interaction with and stimulation by G(M2) activator.

Sarkar, S, Miwa, N, Kominami, H, Igarashi, N, Hayashi, S, Okada, T, ...

We have previously reported that a heat-stable activator for ganglioside metabolism, G(M2) activator, potently stimulates ADP-ribosylation factor (ARF)-dependent phospholipase D (PLD) activity...

Biogenesis of membrane lipoproteins in Escherichia coli.

Wu, H C, Lai, J S, Hayashi, S, Giam, C Z

Globomycin-resistant mutants of Escherichia coli have been isolated and partially characterized. Approximately 2-5% of these mutants synthesize structurally altered Braun's lipoprotein. The majority...

Close associations between prevalences of dominantly inherited spinocerebellar ataxias with CAG-repeat expansions and frequencies of large normal CAG alleles in Japanese and Caucasian populations.

Takano, H, Cancel, G, Ikeuchi, T, Lorenzetti, D, Mawad, R, Stevanin, G, ...

To test the hypothesis that the frequencies of normal alleles (ANs) with a relatively large number of CAG repeats (large ANs) are related to the prevalences of the dominant spinocerebellar ataxias...

Biosynthesis of Bacillus licheniformis penicillinase in Escherichia coli and in Bacillus subtilis.

Total parathyroidectomy with forearm autograft for secondary hyperparathyroidism in chronic renal failure.

Modified prepenicillinase was accumulated in both Escherichia coli and Bacillus subtilis treated with globomycin. Although the inhibitions of processings of prepenicillinase and prolipoprotein by...

Takagi, H, Tominaga, Y, Tanaka, Y, Uchida, K, Orihara, A, Yamada, N, ...

One hundred twenty-eight patients with chronic renal failure and secondary hyperparathyroidism underwent total parathyroidectomy with forearm autograft. Postoperative clinical improvement was...

Problems associated with the EEA stapling technique for esophagojejunostomy after total gastrectomy.

Kataoka, M, Masaoka, A, Hayashi, S, Honda, H, Hotta, T, Niwa, T, ...

Since November 1980, the EEA stapling device has been used to create an end-to-side esophagojejunostomy in 238 patients who underwent total gastrectomy. Failures in the stapling technique were...

A histologically distinctive interstitial pneumonia induced by overexpression of the interleukin 6, transforming growth factor beta 1, or platelet-derived growth factor B gene.

Yoshida, M, Sakuma, J, Hayashi, S, Abe, K, Saito, I, Harada, S, ...

Interstitial pneumonia is characterized by alveolitis with resulting fibrosis of the interstitium. To determine the relevance of humoral factors in the pathogenesis of interstitial pneumonia, we...

Antizyme protects against abnormal accumulation and toxicity of polyamines in ornithine decarboxylase-overproducing cells.

Suzuki, T, He, Y, Kashiwagi, K, Murakami, Y, Hayashi, S, Igarashi, K

Exposure of ornithine decarboxylase (ODC; L-ornithine carboxy-lyase, EC 4.1.1.17)-overproducing mouse FM3A cells to micromolar levels of spermine or spermidine caused abnormal accumulation and...

Overexpression of Cu-Zn superoxide dismutase in Drosophila does not affect life-span.

Immunoglobulin G1 Antibody Response to Helicobacter pylori Heat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

Aging and disease processes may be due to deleterious and irreversible changes produced by free radical reactions. The enzyme copper-zinc superoxide dismutase (Cu-Zn SOD; superoxide:superoxide...

Ishii, E., Yokota, K., Sugiyama, T., Fujinaga, Y., Ayada, K., Hokari, I., ...

Gastric mucosa-associated lymphoid tissue (MALT) lymphoma is related to Helicobacter pylori infection. Specifically, it has been pointed out that pathogenesis of MALT lymphoma involves the 60-kDa...

Functional modulation of estrogen receptor by redox state with reference to thioredoxin as a mediator.

Hayashi, S, Hajiro-Nakanishi, K, Makino, Y, Eguchi, H, Yodoi, J, Tanaka, H

Redox regulation of transcription factors has recently been demonstrated for AP-1, NF-kappaB, Sp-1 and glucocorticoid receptor in vitro and in vivo. The redox state in estrogen-dependent cells...

Regulation of estrogen receptor alpha gene mediated by promoter B responsible for its enhanced expressionin human breast cancer.

Tanimoto, K, Eguchi, H, Yoshida, T, Hajiro-Nakanishi, K, Hayashi, S

We have previously reported that transcription from a distal promoter (promoter B) of the estrogen receptor alpha (ERalpha) gene is responsible for the increased expression of ERalpha in human breast...

Reactivities of Lewis antigen monoclonal antibodies with the lipopolysaccharides of Helicobacter pylori strains isolated from patients with gastroduodenal diseases in Japan.

Amano, K, Hayashi, S, Kubota, T, Fujii, N, Yokota, S

We have examined the reactivity of monoclonal antibodies (MAbs) specific for Lewis antigens (Le(x), Le(y), Le(a), and Le(b)) with Helicobacter pylori lipopolysaccharides (LPS) by immunoblot analysis...

In vitro effects of beta-lactams combined with beta-lactamase inhibitors against methicillin-resistant Staphylococcus aureus.

In vitro inhibition of the infectivity and replication of human immunodeficiency virus type 1 by combination of antiretroviral 2',3'-dideoxynucleosides and virus-binding inhibitors.

The effects of combinations of beta-lactams with two beta-lactamase inhibitors, sulbactam and clavulanic acid, were determined in vitro against 22 clinical isolates of methicillin-resistant...

Hayashi, S, Fine, R L, Chou, T C, Currens, M J, Broder, S, Mitsuya, H

We tested the in vitro inhibitory activities of three 2',3'-dideoxynucleosides and two inhibitors of viral binding in combinations against the infectivity and cytopathic effect of human...

Cyclobut-A and cyclobut-G, carbocyclic oxetanocin analogs that inhibit the replication of human immunodeficiency virus in T cells and monocytes and macrophages in vitro.

Hayashi, S, Norbeck, D W, Rosenbrook, W, Fine, R L, Matsukura, M, Plattner, J J, ...

Two newly synthesized carbocyclic oxetanocin analogs, (+/-)-9-[(1 beta,2 alpha,3 beta)-2,3-bis(hydroxymethyl)-1-cyclobutyl]adenine (cyclobut-A) and (+/-)-9-[(1 beta,2 alpha,3...

Simple assay of beta-lactamase with agar medium containing a chromogenic cephalosporin, pyridinium-2-azo-p-dimethylaniline chromophore (PADAC).

Low antigenicity of the polysaccharide region of Helicobacter pylori lipopolysaccharides derived from tumors of patients with gastric cancer.

A new beta-lactamase assay method with agar plates containing pyridinium-2-azo-p-dimethylaniline chromophore (PADAC) (50 microM), a beta-lactamase-labile, chromogenic cephalosporin, was examined. On...

Yokota, S, Amano, K, Hayashi, S, Fujii, N

We have examined the antibody response to Helicobacter pylori lipopolysaccharide (LPS) during natural infection in humans. The sera of over 70% of H. pylori-infected individuals were found to contain...

Distribution of cefpirome (HR 810) to exudate in the croton oil-induced rat granuloma pouch and its therapeutic effects on experimental infections in the pouch.

Arai, S, Kobayashi, S, Hayashi, S, Sakaguchi, T

Therapeutic effects of intravenously administered cefpirome on experimental bacterial infections in croton oil-induced rat granuloma pouches were compared with those of ceftazidime, moxalactam,...

Beta-lactamase stability of cefpirome (HR 810), a new cephalosporin with a broad antimicrobial spectrum.

Kobayashi, S, Arai, S, Hayashi, S, Fujimoto, K

Cefpirome was highly stable to hydrolysis by various beta-lactamases, although it was hydrolyzed to some extent by R plasmid-mediated penicillinase of Richmond-Sykes type Va/b and by chromosomal...

Preparation and regeneration of bacteriophage PL-1 enzyme-induced Lactobacillus casei protoplasts.

Watanabe, K, Hayashida, M, Nakashima, Y, Hayashi, S

Lactobacillus casei ATCC 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the PL-1 phage lysates of this bacterium, at 37 degrees C for 1.5 h in 50 mM...

Synthesis and export of the outer membrane lipoprotein in Escherichia coli mutants defective in generalized protein export.

Watanabe, T, Hayashi, S, Wu, H C

Export of the outer membrane lipoprotein in Escherichia coli was examined in conditionally lethal mutants that were defective in protein export in general, including secA, secB, secC, and secD....

Effect of a mutation preventing lipid modification on localization of the pCloDF13-encoded bacteriocin release protein and on release of cloacin DF13.

Luirink, J, Hayashi, S, Wu, H C, Kater, M M, De Graaf, F K, Oudega, B

The pCloDF13-encoded bacteriocin release protein (BRP; Mr 2,871) is essential for the translocation of cloacin DF13 across the cell envelope of producing Escherichia coli cells. Overproduction of...

Lipid modification of Escherichia coli penicillin-binding protein 3.

Hayashi, S, Hara, H, Suzuki, H, Hirota, Y

The primary structure of penicillin-binding protein 3 (PBP 3), an essential enzyme for cell division in Escherichia coli, was deduced from the nucleotide sequence of the ftsI gene (M. Nakamura, I. N....

Processing of Bacillus licheniformis penicillinases lacking a lipoprotein modification site in Escherichia coli.

Hayashi, S, Chang, S Y, Chang, S, Wu, H C

We have previously shown that the penP Ser-27 prepenicillinase is processed into two forms, Ser-35-penicillinase and Asn-29 penicillinase. Two new penicillinase mutants, penP Ser-27 Pro-28 and penP...

Accumulation of prolipoprotein in Escherichia coli mutants defective in protein secretion.

Globomycin sensitivity of Escherichia coli and Salmonella typhimurium: effects of mutations affecting structures of murein lipoprotein.

The export of lipoprotein has been found to be affected in both secA and secY mutants of Escherichia coli which are defective in the secretion of a number of outer membrane and periplasmic proteins....

Lai, J S, Philbrick, W M, Hayashi, S, Inukai, M, Arai, M, Hirota, Y, ...

The sensitivity of strains of Escherichia coli and Salmonella typhimurium to globomycin is increased in mutants defective in the lipopolysaccharide structure. E. coli mutants altered in the...

Biosynthesis of Bacillus licheniformis penicillinase in Escherichia coli and in Bacillus subtilis.

Temperature-sensitive mutations in Drosophila melanogaster,I. Relative frequencies among gamma-ray and chemically induced sex-linked recessive lethals and semilethals.

Modified prepenicillinase was accumulated in both Escherichia coli and Bacillus subtilis treated with globomycin. Although the inhibitions of processings of prepenicillinase and prolipoprotein by...

Suzuki, D T, Piternick, L K, Hayashi, S, Tarasoff, M, Baillie, D, Erasmus, U

Structural rearrangement of integrated hepatitis B virus DNA as well as cellular flanking DNA is present in chronically infected hepatic tissues.

Takada, S, Gotoh, Y, Hayashi, S, Yoshida, M, Koike, K

Cellular DNAs from human livers chronically infected with hepatitis B virus (HBV) were analyzed by Southern blot hybridization for the presence of integrated HBV DNA. In 15 of 16 chronically infected...

Isolation and characteristics of collagenolytic enzyme produced by Candida albicans.

Kaminishi, H, Hagihara, Y, Hayashi, S, Cho, T

In media containing collagen as the nitrogen source, the pathogenic yeast Candida albicans secreted a collagenolytic enzyme. Purification of the enzyme from a culture filtrate was achieved by...

Augmented production of colony-stimulating factor in C3H/HeN mice immunized with Nocardia rubra cell wall skeleton.

Hayashi, S, Masuno, T, Hosoe, S, Kawase, I, Sakatani, M, Ogura, T, ...

C3H/HeN mice subcutaneously injected repeatedly with Nocardia rubra cell wall skeleton (N-CWS) acquired cellular immunity against N-CWS. In these N-CWS-immunized mice, the serum colony-stimulating...

Prevalence of antibodies to Coxiella burnetii in Japan.

Htwe, K K, Yoshida, T, Hayashi, S, Miyake, T, Amano, K, Morita, C, ...

We evaluated the prevalence of Coxiella burnetii antibodies in 626 human serum samples (275 from veterinarians, 107 from meat-processing workers, 184 from respiratory-disorder patients, and 60 from...

Adenallene and cytallene: acyclic-nucleoside analogues that inhibit replication and cytopathic effect of human immunodeficiency virus in vitro.

Hayashi, S, Phadtare, S, Zemlicka, J, Matsukura, M, Mitsuya, H, Broder, S

Although several antiretroviral compounds are already known, almost no acyclic nucleoside derivatives lacking an oxacyclopentane have been reported to exert significant inhibition against human...

Drosophila Cu-Zn superoxide dismutase cDNA sequence.

Growth Stasis by Accumulated l-α-Glycerophosphate in Escherichia coli

Cozzarelli, N. R., Koch, J. P., Hayashi, S., Lin, E. C. C.

Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), J. P. Koch, S. Hayashi, and E. C. C. Lin. Growth stasis by accumulated l-α-glycerophosphate in Escherichia coli. J. Bacteriol....

Genes for tRNALys5 from Drosophila melanogaster.

DeFranco, D, Burke, K B, Hayashi, S, Tener, G M, Miller, R C, Söll, D

The sequences of two cloned genes from Drosophila which hybridize with tRNALys5 are reported. One gene, in plasmid pDt39, has a sequence which corresponds to the sequence of tRNA. The other gene, in...

Nucleotide sequence of a pseudogene for rat ornithine decarboxylase.

Kanamoto, R, Yoshimura, M, Hayashi, S, Oka, T

Exon skipping by mutation of an authentic splice site of c-kit gene in W/W mouse.

Hayashi, S, Kunisada, T, Ogawa, M, Yamaguchi, K, Nishikawa, S

The murine mutation dominant white spotting (W) is in the proto-oncogene, c-kit. The receptor tyrosine kinase encoded by this gene has pleiotropic effects on murine development including hemopoietic...

The sequence of the Cu-Zn superoxide dismutase gene of Drosophila.