Direct, Non-Destructive Imaging of Magnetization in a Spin-1 Bose Gas (2005)
Higbie, J. M., Sadler, L. E., Inouye, S., Chikkatur, A. P., Leslie, S. R., Moore, K. L., ...
Polarization-dependent phase-contrast imaging is used to spatially resolve the magnetization of an optically trapped ultracold gas. This probe is applied to Larmor precession of degenerate and...
Cross-Dimensional relaxation in Bose-Fermi mixtures (2005)
Goldwin, J., Inouye, S., Olsen, M. L., Jin, D. S.
We consider the equilibration rate for fermions in Bose-Fermi mixtures undergoing cross-dimensional rethermalization. Classical Monte Carlo simulations of the relaxation process are performed over a...
Effect of Magnesium as an Alloying Element in Inconel 718. (2005)
Couts,W. H. , Jr., Inouye,S., Saul,G.
Six 50-lb heats of Inconel 718 were prepared with magnesium contents ranging from 1 to 350 ppm. Half were vacuum arc remelted and half were arc-remelted under 1/2 atm of argon. The homogenized ingots...
Observation of Heteronuclear Feshbach Resonances in a Bose-Fermi Mixture (2004)
Inouye, S., Goldwin, J., Olsen, M. L., Ticknor, C., Bohn, J. L., Jin, D. S.
Three magnetic-field induced heteronuclear Feshbach resonances were identified in collisions between bosonic 87Rb and fermionic 40K atoms in their absolute ground states. Strong inelastic loss from...
Measurement of the interaction strength in a Bose-Fermi mixture with 87Rb and 40K (2004)
Goldwin, J., Inouye, S., Olsen, M. L., Newman, B., DePaola, B. D., Jin, D. S.
A quantum degenerate, dilute gas mixture of bosonic and fermionic atoms was produced using 87Rb and 40K. The onset of degeneracy was confirmed by observing the spatial distribution of the gases after...
Sodium Bose-Einstein Condensates in the F=2 State in a Large-volume Optical Trap (2002)
Görlitz, A., Gustavson, T. L., Leanhardt, A. E., Löw, R., Chikkatur, A. P., Gupta, S., ...
We have investigated the properties of Bose-Einstein condensates of sodium atoms in the upper hyperfine ground state in a purely optical trap. Condensates in the high-field seeking $| F=2, m_F=-2>$...
Realization of Bose-Einstein condensates in lower dimensions (2001)
Gorlitz, A., Vogels, J. M., Leanhardt, A. E., Raman, C., Gustavson, T. L., Abo-Shaeer, J. R., ...
Bose-Einstein condensates of sodium atoms have been prepared in optical and magnetic traps in which the energy-level spacing in one or two dimensions exceeds the interaction energy between atoms,...
Observation of vortex phase singularities in Bose-Einstein condensates (2001)
Inouye, S., Gupta, S., Rosenband, T., Chikkatur, A. P., Görlitz, A., Gustavson, T. L., ...
We have observed phase singularities due to vortex excitation in Bose-Einstein condensates. Vortices were created by moving a laser beam through a condensate. They were observed as dislocations in...
Amplification of light and atoms in a Bose--Einstein condensate (2000)
Inouye, S., Loew, R. F., Gupta, S., Pfau, T., Gorlitz, A., Gustavson, T. L., ...
A Bose-Einstein condensate illuminated by a single off-resonant laser beam (``dressed condensate'') shows a high gain for matter waves and light. We have characterized the optical and atom-optical...
Suppression and enhancement of impurity scattering in a Bose-Einstein condensate (2000)
Chikkatur, A. P., Gorlitz, A., Stamper-Kurn, D. M., Inouye, S., Gupta, S., Ketterle, W.
Impurity atoms propagating at variable velocities through a trapped Bose-Einstein condensate were produced using a stimulated Raman transition. The redistribution of momentum by collisions between...
Excitation of phonons in a Bose-Einstein condensate by light scattering (1999)
Stamper-Kurn, D. M., Chikkatur, A. P., Görlitz, A., Inouye, S., Gupta, S., Pritchard, D. E., ...
Stimulated small-angle light scattering was used to measure the structure factor of a Bose-Einstein condensate in the phonon regime. The excitation strength for phonons was found to be significantly...
Quantum tunneling across spin domains in a Bose-Einstein condensate (1999)
Stamper-Kurn, D. M., Miesner, H. -J., Chikkatur, A. P., Inouye, S., Stenger, J., Ketterle, W.
Quantum tunneling was observed in the decay of metastable spin domains in gaseous Bose-Einstein condensates. A mean-field description of the tunneling was developed and compared with measurement. The...
Bragg spectroscopy of a Bose-Einstein condensate (1999)
Stenger, J., Inouye, S., Chikkatur, A. P., Stamper--Kurn, D. M., Pritchard, D. E., Ketterle, W.
Properties of a Bose-Einstein condensate were studied by stimulated, two-photon Bragg scattering. The high momentum and energy resolution of this method allowed a spectroscopic measurement of the...
Spin domains in ground state spinor Bose-Einstein condensates (1999)
Stenger, J., Inouye, S., Stamper-Kurn, D. M., Miesner, H. -J., Chikkatur, A. P., Ketterle, W.
Bose-Einstein condensates of dilute atomic gases, characterized by a macroscopic population of the quantum mechanical ground state, are a new, weakly interacting quantum fluid. In most experiments...
Strongly enhanced inelastic collisions in a Bose-Einstein condensate near Feshbach resonances (1999)
Stenger, J., Inouye, S., Andrews, M. R., Miesner, H. -J., Stamper-Kurn, D. M., Ketterle, W.
The properties of Bose-Einstein condensed gases can be strongly altered by tuning the external magnetic field near a Feshbach resonance. Feshbach resonances affect elastic collisions and lead to the...
Observation of metastable states in spinor Bose-Einstein condensates (1998)
Miesner, H. -J., Stamper-Kurn, D. M., Stenger, J., Inouye, S., Chikkatur, A., Ketterle, W.
Bose-Einstein condensates have been prepared in long-lived metastable excited states. Two complementary types of metastable states were observed. The first is due to the immiscibility of multiple...
The Effect of Cold Drawing on the Elastic Modulus of Aluminum Composite Driveshaft Tube. (1998)
Klimowicz, T. F., Dixon, W., Inouye, S.
This paper concerned mmc in some important way and contained information about, utilized, or discussed these subjects: basic topics:, dr mmc, experimental, mmc materials and material types:,...
Reversible Formation of a Bose-Einstein Condensate (1998)
Stamper-Kurn, D. M., Miesner, H. -J., Chikkatur, A. P., Inouye, S., Stenger, J., Ketterle, W.
We present a method of adiabatically changing the local phase-space density of an ultracold gas, using a combination of magnetic and optical forces. Applying this method, we observe phase-space...
Stamper-Kurn, D. M., Miesner, H. -J., Inouye, S., Andrews, M. R., Ketterle, W.
Collective excitations of a dilute Bose gas were probed above and below the Bose-Einstein condensation temperature. The temperature dependencies of the frequency and damping rates of condensate...
Optical Confinement of a Bose-Einstein Condensate (1997)
Stamper-Kurn, D. M., Andrews, M. R., Chikkatur, A. P., Inouye, S., Miesner, H. -J., Stenger, J., ...
Bose-Einstein condensates of sodium atoms have been confined in an optical dipole trap using a single focused infrared laser beam. This eliminates the restrictions of magnetic traps for further...
Ogawa, H, Inouye, S, Tsuji, F I, Yasuda, K, Umesono, K
The localization, trafficking, and fluorescence of Aequorea green fluorescent protein (GFP) in cultured vertebrate cells transiently transfected with GFP cDNA were studied. Fluorescence of GFP in UV...
Bagby, S, Harvey, T S, Eagle, S G, Inouye, S, Ikura, M
The solution structure of Ca(2+)-loaded protein S (M(r) 18,792) from the Gram-negative soil bacterium Myxococcus xanthus has been determined by multidimensional heteronuclear NMR spectroscopy....
Ohshima, A, Inouye, S, Inouye, M
Gene duplication through cDNA synthesis by reverse transcriptase is believed to have played an important role in the diversification of genomes during evolution. Here, we demonstrate that a genomic...
Imaging of luciferase secretion from transformed Chinese hamster ovary cells.
Inouye, S, Ohmiya, Y, Toya, Y, Tsuji, F I
The blue luminescence characteristic of the marine ostracod crustacean Vargula hilgendorfii is from a simple, but highly specific, enzyme-substrate reaction. Light is emitted by the oxidation of...
Two independent retrons with highly diverse reverse transcriptases in Myxococcus xanthus.
Inouye, S, Herzer, P J, Inouye, M
A reverse transcriptase (RT) was recently found in Myxococcus xanthus, a Gram-negative soil bacterium. This RT has been shown to be associated with a chromosomal region designated a retron...
Hsu, M Y, Inouye, M, Inouye, S
The region (retron-Ec67) required for the biosynthesis of a branched-RNA-linked multicopy single-stranded DNA (msDNA-Ec67) from a clinical isolate of Escherichia coli was mapped at a position...
Miyazawa, H., Bannai, H., Yanase, T., Morita, C., Satoh, S., Sugiyama, J., ...
A reverse-sandwich enzyme-linked immunosorbent assay (ELISA), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (VT) in human and...
In vitro activity of ME1401, a new antifungal agent.
Yamaguchi, H, Uchida, K, Hiratani, T, Hara, T, Fukuyasu, H, Kazuno, Y, ...
The in vitro antifungal activity of ME1401, a potential topical antifungal agent, was compared with that of haloprogin, clotrimazole, miconazole, tolnaftate, and ciclopirox olamine by using an agar...
Shimamoto, T, Shimada, M, Inouye, M, Inouye, S
Bacterial reverse transcriptase is responsible for the synthesis of multicopy single-stranded DNA (msDNA). Reverse transcriptases from retron-Ec73 and retron-Ec107 do not contain an RNase H domain....
Two recA genes in Myxococcus xanthus.
Norioka, N, Hsu, M Y, Inouye, S, Inouye, M
Two recA genes, recA1 and recA2, in Myxococcus xanthus were cloned by using the recA gene of Escherichia coli, and their DNA sequences were determined. On the basis of deduced amino acid sequences,...
Germination of myxospores from the fruiting bodies of Myxococcus xanthus.
Otani, M, Inouye, M, Inouye, S
Germination of myxospores from fruiting bodies of Myxococcus xanthus was examined under a light microscope as well as by analyzing the incorporation of [3H]uracil into the RNA fraction. Efficient...
MlpA, a lipoprotein required for normal development of Myxococcus xanthus.
Hanlon, W A, Martinez-Canamero, M, Inouye, M, Inouye, S
The mlpA gene encoding a 236-residue polypeptide has been identified immediately downstream of the oar gene of Myxococcus xanthus (M. Martinez-Canamero, J. Munoz-Dorado, E. Farez-Vidal, M. Inouye,...
Tojo, N, Sanmiya, K, Sugawara, H, Inouye, S, Komano, T
Mx8 is a generalized transducing phage that infects Myxococcus xanthus cells. This phage is lysogenized in M. xanthus cells by the integration of its DNA into the host chromosome through...
Pkn2 is a putative transmembrane protein serine/threonine kinase required for normal development of Myxococcus xanthus. The effect of Pkn2 overexpression on development of M. xanthus was examined by...
msDNA-Ec48, the smallest multicopy single-stranded DNA from Escherichia coli.
Mao, J R, Inouye, S, Inouye, M
Previously we have reported a novel bacterial reverse transcriptase (RT) from Escherichia coli ECOR58 strains in which the YXDD box was replaced with LVDD (J.-R. Mao, S. Inouye, and M. Inouye,...
Inouye, S, Goi, H, Watanabe, T, Hara, T, Miyauchi, K, Yoshida, T, ...
The in vitro and in vivo antibacterial activities of MT-141 were compared with those of cefoxitin, cefmetazole, moxalactam, cefotaxime, and cefoperazone. The MICs of MT-141 for 90% of bacterial...
Matsuhashi, Y, Yoshida, T, Hara, T, Kazuno, Y, Inouye, S
When compared with astromicin, amikacin, gentamicin, and sisomicin, dactimicin was similar to astromicin in in vitro activity and was more active than amikacin and gentamicin against the clinical...
Autophosphorylation of nucleoside diphosphate kinase from Myxococcus xanthus.
Muñoz-Dorado, J, Almaula, N, Inouye, S, Inouye, M
The nucleoside diphosphate kinase (NDP kinase) from Myxococcus xanthus has been purified to homogeneity and crystallized (J. Munoz-Dorado, M. Inouye, and S. Inouye, J. Biol. Chem. 265:2702-2706,...
The lon gene of Escherichia coli is known to encode protease La, an ATP-dependent protease associated with cellular protein degradation. A lon gene homolog from Myxococcus xanthus, a soil bacterium...
A new putative sigma factor of Myxococcus xanthus.
A third putative sigma factor gene, sigC, has been isolated from Myxococcus xanthus by using the sigA gene (formerly rpoD of M. xanthus) as a probe. The nucleotide sequence of sigC has been...
Myxococcus xanthus contains two genes (lonV and lonD) homologous to the Escherichia coli lon gene for an ATP-dependent protease. We found that the lonD gene encodes a 90-kDa protein consisting of 827...
Oar, a 115-kilodalton membrane protein required for development of Myxococcus xanthus.
Martinez-Canamero, M, Munoz-Dorado, J, Farez-Vidal, E, Inouye, M, Inouye, S
Myxococcus xanthus is a developmental gram-negative bacterium which forms multicellular fruiting bodies upon nutrient starvation. This bacterium was found to contain a 115-kDa membrane protein which...
Zenno, S, Saigo, K, Kanoh, H, Inouye, S
The gene encoding the major NAD(P)H-flavin oxidoreductase (flavin reductase) of the luminous bacterium Vibrio fischeri ATCC 7744 was isolated by using synthetic oligonucleotide probes corresponding...
Hsu, M Y, Xu, C, Inouye, M, Inouye, S
To determine the evolutional relationship of bacterial retroelements of Myxococcus xanthus and Stigmatella aurantiaca, the nucleotide sequence of 3,060 bases encompassing msr, msd, and the upstream...
Zhang, W, Munoz-Dorado, J, Inouye, M, Inouye, S
Myxococcus xanthus is a gram-negative bacterium which, upon starvation, undergoes a spectacular developmental cycle culminating in the formation of spore-filled fruiting bodies. We recently...
A unique repetitive DNA sequence in the Myxococcus xanthus genome.
Fujitani, S, Komano, T, Inouye, S
We found a novel type of repetitive DNA sequence in the Myxococcus xanthus genome. The first repetitive sequence is located in the spacer region between the ops and tps genes. We cloned five other...
Protein U, a late-developmental spore coat protein of Myxococcus xanthus, is a secretory protein.
Gollop, R, Inouye, M, Inouye, S
Protein U is a spore coat protein produced at the late stage of development of Myxococcus xanthus. This protein was isolated from developmental cells, and its amino-terminal sequence was determined....
A new multicopy single-stranded DNA (msDNA-Ec73) was found in a clinical strain of Escherichia coli. Retron-Ec73, consisting of an msDNA-coding region and the gene for reverse transcriptase (RT), was...
Lampson, B C, Inouye, M, Inouye, S
Twenty different isolates of the soil bacterium Myxococcus xanthus were examined for the presence of multicopy single-stranded DNA (msDNA)-producing retroelements, or retrons. Each strain was...
Cloning and DNA sequence of the gene coding for the major sigma factor from Myxococcus xanthus.
The gene for a sigma factor (rpoD) was cloned from Myxococcus xanthus, a soil bacterium which differentiates to form fruiting bodies upon starvation for nutrients. The DNA sequence of the gene was...
Mutations that affect production of branched RNA-linked msDNA in Myxococcus xanthus.
Dhundale, A, Furuichi, T, Inouye, M, Inouye, S
A deletion mutation of the gene (msd-msr) for the branched RNA-linked msDNA of Myxococcus xanthus was constructed by replacing the chromosomal 0.7-kilobase (kb) SmaI-XhoI fragment encompassing...
Overproduction of the xylS gene product and activation of the xylDLEGF operon on the TOL plasmid.
Inouye, S, Nakazawa, A, Nakazawa, T
The effect of high-level expression of the regulatory gene xylS of the Pseudomonas putida TOL plasmid on the activation of the xylDLEGF operon was investigated in Escherichia coli. The xylS gene was...
Furuichi, T, Inouye, M, Inouye, S
A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for...
Distribution of multicopy single-stranded DNA among myxobacteria and related species.
Dhundale, A R, Furuichi, T, Inouye, S, Inouye, M
Multicopy single-stranded DNA (msDNA) is a short single-stranded linear DNA originally discovered in Myxococcus xanthus and subsequently found in Stigmatella aurantiaca. It exists at an estimated 500...
Nucleotide sequence of a DNA segment promoting transcription in Pseudomonas putida.
Inouye, S, Asai, Y, Nakazawa, A, Nakazawa, T
A DNA segment that promotes gene expression in Pseudomonas putida was identified in pTN8, a mutant plasmid of an RP4-TOL recombinant. A promoter on the segment was cloned with a promoter-probe vector...
Komano, T, Furuichi, T, Teintze, M, Inouye, M, Inouye, S
A deletion mutation of the gene for protein S (tps), a development-specific protein of Myxococcus xanthus, was constructed. No significant differences in the process of fruiting body formation or the...
Tsuruoka, T, Tamura, A, Miyata, A, Takei, T, Iwamatsu, K, Inouye, S, ...
Certain D-amino acids, such as D-methionine and D-cystine, were incorporated into cells of Escherichia coli under conditions inhibiting protein and cell wall synthesis. Part of the radioactivity of...
Correia, F F, Inouye, S, Inouye, M
Two-dimensional heteroduplex mapping of Neisseria gonorrhoeae genomic DNA revealed a number of spots, indicating the existence of repetitive sequences. When one of the spots was extracted and used as...
Inouye, S, Nakazawa, A, Nakazawa, T
The xylDEGF operon and the regulatory gene xylS of the TOL plasmid found in Pseudomonas putida mt-2 were cloned onto Escherichia coli vector plasmids. A 9.5-kilobase fragment, derived from the TOL...
Development-specific protein S of Myxococcus xanthus: purification and characterization.
Inouye, S, Harada, W, Zusman, D, Inouye, M
Protein S, a development-specific protein of Myxococcus xanthus, was purified from the cells of a late stage of development and crystallized. Its circular dichroism spectra indicated that protein S...
Molecular cloning of TOL genes xylB and xylE in Escherichia coli.
Inouye, S, Nakazawa, A, Nakazawa, T
The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in...
Toh-e, A, Inouye, S, Oshima, Y
pho4 mutants of Saccharomyces cerevisiae, although rare among phosphatase-negative mutants isolated from wild-type strains, were isolated efficiently from pho80, pho85, or pho80 pho85 strains. The...
Komano, T, Inouye, S, Inouye, M
Myxococcus xanthus was pulse-labeled with [3H]thymidine immediately after germination of dimethyl sulfoxide-induced spores. The restriction enzyme digests of the total chromosomal DNA from the...
Teintze, M, Thomas, R, Furuichi, T, Inouye, M, Inouye, S
The ops and tps genes of Myxococcus xanthus have ca. 90% DNA and amino acid sequence homology and are in the same orientation separated by a spacer region of only 1.4 kilobases. The products of the...
Inouye, S, Nakazawa, A, Nakazawa, T
The xylR gene is a regulatory gene on the TOL plasmid, which acts in a positive manner on xyl operons for degradation of toluene and xylenes in Pseudomonas putida. A DNA fragment containing the xylR...
Phosphorylation and methylation of proteins during Myxococcus xanthus spore formation.
Komano, T, Brown, N, Inouye, S, Inouye, M
Post-translational modification of proteins was examined during the life cycle of Myxococcus xanthus. A specific pattern of protein phosphorylation was observed in vegetative cells. When spore...
Amino acid replacement in a mutant lipoprotein of the Escherichia coli outer membrane.
Inouye, S, Lee, N, Inouye, M, Wu, H C, Suzuki, H, Nishimura, Y, ...
The primary structure of a mutant lipoprotein of the outer membrane of Escherichia coli was investigated. This mutant was previously described as a mutant that forms a dimer of the lipoprotein by an...