Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr.
Cheung, A L, Koomey, J M, Butler, C A, Projan, S J, Fischetti, V A
A single insertion of transposon Tn917LTV1 into the chromosome of a Staphylococcus aureus clinical isolate, strain DB, resulted in a pleiotropic effect on the expression of a number of extracellular...
Alksne, L. E., Burgio, P., Hu, W., Feld, B., Singh, M. P., Tuckman, M., ...
Protein secretion is an essential process for bacterial growth, yet there are few if any antimicrobial agents which inhibit secretion. An in vivo, high-throughput screen to detect secretion...
Dunman, P. M., Murphy, E., Haney, S., Palacios, D., Tucker-Kellogg, G., Wu, S., ...
The advent of transcription profiling technologies has provided researchers with an unprecedented ability to study biological processes. Accordingly, a custom-made Affymetrix GeneChip, constituting...
Global Regulation of Staphylococcus aureus Genes by Rot
Saïd-Salim, B., Dunman, P. M., McAleese, F. M., Macapagal, D., Murphy, E., McNamara, P. J., ...
Staphylococcus aureus produces a wide array of cell surface and extracellular proteins involved in virulence. Expression of these virulence factors is tightly controlled by numerous regulatory loci,...
Bradford, P A, Urban, C, Jaiswal, A, Mariano, N, Rasmussen, B A, Projan, S J, ...
Four ceftazidime-resistant Escherichia coli strains were isolated from elderly nursing home patients in a New York hospital during 1993. Strains MCQ-2, MCQ-3, and MCQ-4 were determined to be...
Bradford, P A, Urban, C, Mariano, N, Projan, S J, Rahal, J J, Bush, K
Six Escherichia coli and 12 Klebsiella pneumoniae isolates from a single hospital expressed a common beta-lactamase with a pI of approximately 9.0 and were resistant to cefoxitin and cefotetan (MIC...
Nesin, M, Svec, P, Lupski, J R, Godson, G N, Kreiswirth, B, Kornblum, J, ...
This report describes the cloning and sequencing of a chromosomally encoded tetracycline resistance determinant from a clinical isolate of methicillin-resistant Staphylococcus aureus. On the basis of...
Cheung, A I, Projan, S J, Edelstein, R E, Fischetti, V A
Septicemia due to Staphylococcus aureus often begins as a focal infection (e.g., colonized wounds or catheters) from which the organism gains access to the bloodstream. On the basis of recent data...
Effect of glycerol monolaurate on bacterial growth and toxin production.
Schlievert, P M, Deringer, J R, Kim, M H, Projan, S J, Novick, R P
Glycerol monolaurate (GML) is a naturally occurring surfactant that has potential use as an additive to tampons and wound dressings to reduce the incidence of certain bacterial toxin-mediated...
Plasmids of the pT181 family show replication-specific initiator protein modification.
Rasooly, A, Projan, S J, Novick, R P
The rolling circle plasmids of Staphylococcus aureus regulate their replication by controlling initiator (Rep) protein synthesis. It was demonstrated recently that the pT181 initiator protein RepC is...
Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr.
To evaluate the effect of a sar mutation on the agr locus, Northern (RNA) blotting was performed to determine the levels of RNAIII, the agr regulatory molecule, in two isogenic pairs of...
Projan, S J, Brown-Skrobot, S, Schlievert, P M, Vandenesch, F, Novick, R P
Glycerol monolaurate (GML) is a naturally occurring surfactant that is used widely as an emulsifier in the food and cosmetics industries and is generally regarded as lacking in important biological...
The Staphylococcus aureus plasmid pC221, a 4.6-kilobase multicopy chloramphenicol resistance plasmid that forms plasmid-protein relaxation complexes, was mobilized for transfer by the conjugative...
We present data which indicate that (i) the origin of replication of plasmids pT181 and pC221 can also function as termination signals; (ii) termination of replication occurs when a round of...
Translational fusion with a secretory enzyme as an indicator.
Wang, P Z, Projan, S J, Leason, K R, Novick, R P
A novel type of translational fusion system has been developed by using a secretory protein, staphylococcal beta-lactamase, as an indicator. The beta-lactamase structural gene was modified to provide...
Projan, S J, Monod, M, Narayanan, C S, Dubnau, D
A naturally occurring plasmid from Bacillus subtilis, pIM13, codes for constitutively expressed macrolide-lincosamide-streptogramin B (MLS) resistance, is stably maintained at a high copy number, and...
Isolation and analysis of multicopy extragenic suppressors of dnaA mutations.
Recombinant plasmids were constructed from restriction enzyme digests of Escherichia coli chromosomal deoxyribonucleic acid and pMB9 plasmid deoxyribonucleic acid and selected for correction of the...
Nucleotide sequence: the beta-hemolysin gene of Staphylococcus aureus.
Projan, S J, Kornblum, J, Kreiswirth, B, Moghazeh, S L, Eisner, W, Novick, R P
Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus.
Projan, S J, Moghazeh, S, Novick, R P
pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. The plasmid has a copy number of about 25 per cell, and belongs to the inc5 incompatibility group. The...
A temperature-dependent pBR322 copy number mutant resulting from a Tn5 position effect.
Lupski, J R, Projan, S J, Ozaki, L S, Godson, G N
In the process of randomly mutagenizing a recombinant pBR322 clone with transposon Tn5, a high copy number plasmid mutant, pLO88, has been isolated. The copy number phenotype of pLO88 is observed...
Wang, P Z, Projan, S J, Henriquez, V, Novick, R P
The leading strand replication origin of pT181 plasmids consists of two adjacent inverted repeat elements (IR-II and IR-III), which are involved in origin recognition by the initiator (Rep) protein....
Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule.
Novick, R P, Ross, H F, Projan, S J, Kornblum, J, Kreiswirth, B, Moghazeh, S
The production of most toxins and other exoproteins in Staphylococcus aureus is controlled globally by a complex polycistronic regulatory locus, agr. Secretory proteins are up-regulated by agr...
Uses of Staphylococcus aureus GeneChips in Genotyping and Genetic Composition Analysis
Dunman, P. M., Mounts, W., McAleese, F., Immermann, F., Macapagal, D., Marsilio, E., ...
Understanding the relatedness of strains within a bacterial species is essential for monitoring reservoirs of antimicrobial resistance and for epidemiological studies. Pulsed-field gel...
Novick, R P, Adler, G K, Projan, S J, Carleton, S, Highlander, S K, Gruss, A, ...
pT181 is a fully sequenced 4.4-kb 20 copy Tcr plasmid from Staphylococcus aureus. Its replication system involves a unique unidirectional origin embedded in the coding sequence for a...
Control of pT181 replication II. Mutational analysis.
Carleton, S, Projan, S J, Highlander, S K, Moghazeh, S M, Novick, R P
We describe the isolation and analysis of mutations affecting the regulation of Staphylococcus aureus plasmid pT181 replication. Previous results suggested that regulation is achieved by control of...
MgrA Is a Multiple Regulator of Two New Efflux Pumps in Staphylococcus aureus†
Truong-Bolduc, Q. C., Dunman, P. M., Strahilevitz, J., Projan, S. J., Hooper, D. C.
In an analysis of the resistance mechanisms of an mgrA mutant, we identified two genes encoding previously undescribed transporters, NorB and Tet38. norB was 1,392 bp and encoded a predicted 49-kDa...
Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr.
Cheung, A L, Koomey, J M, Butler, C A, Projan, S J, Fischetti, V A
A single insertion of transposon Tn917LTV1 into the chromosome of a Staphylococcus aureus clinical isolate, strain DB, resulted in a pleiotropic effect on the expression of a number of extracellular...
Alksne, L. E., Burgio, P., Hu, W., Feld, B., Singh, M. P., Tuckman, M., ...
Protein secretion is an essential process for bacterial growth, yet there are few if any antimicrobial agents which inhibit secretion. An in vivo, high-throughput screen to detect secretion...
Dunman, P. M., Murphy, E., Haney, S., Palacios, D., Tucker-Kellogg, G., Wu, S., ...
The advent of transcription profiling technologies has provided researchers with an unprecedented ability to study biological processes. Accordingly, a custom-made Affymetrix GeneChip, constituting...
Global Regulation of Staphylococcus aureus Genes by Rot
Saïd-Salim, B., Dunman, P. M., McAleese, F. M., Macapagal, D., Murphy, E., McNamara, P. J., ...
Staphylococcus aureus produces a wide array of cell surface and extracellular proteins involved in virulence. Expression of these virulence factors is tightly controlled by numerous regulatory loci,...
Bradford, P A, Urban, C, Jaiswal, A, Mariano, N, Rasmussen, B A, Projan, S J, ...
Four ceftazidime-resistant Escherichia coli strains were isolated from elderly nursing home patients in a New York hospital during 1993. Strains MCQ-2, MCQ-3, and MCQ-4 were determined to be...
Bradford, P A, Urban, C, Mariano, N, Projan, S J, Rahal, J J, Bush, K
Six Escherichia coli and 12 Klebsiella pneumoniae isolates from a single hospital expressed a common beta-lactamase with a pI of approximately 9.0 and were resistant to cefoxitin and cefotetan (MIC...
Nesin, M, Svec, P, Lupski, J R, Godson, G N, Kreiswirth, B, Kornblum, J, ...
This report describes the cloning and sequencing of a chromosomally encoded tetracycline resistance determinant from a clinical isolate of methicillin-resistant Staphylococcus aureus. On the basis of...
Cheung, A I, Projan, S J, Edelstein, R E, Fischetti, V A
Septicemia due to Staphylococcus aureus often begins as a focal infection (e.g., colonized wounds or catheters) from which the organism gains access to the bloodstream. On the basis of recent data...
Effect of glycerol monolaurate on bacterial growth and toxin production.
Schlievert, P M, Deringer, J R, Kim, M H, Projan, S J, Novick, R P
Glycerol monolaurate (GML) is a naturally occurring surfactant that has potential use as an additive to tampons and wound dressings to reduce the incidence of certain bacterial toxin-mediated...
Plasmids of the pT181 family show replication-specific initiator protein modification.
Rasooly, A, Projan, S J, Novick, R P
The rolling circle plasmids of Staphylococcus aureus regulate their replication by controlling initiator (Rep) protein synthesis. It was demonstrated recently that the pT181 initiator protein RepC is...
Cloning and sequencing of sarA of Staphylococcus aureus, a gene required for the expression of agr.
To evaluate the effect of a sar mutation on the agr locus, Northern (RNA) blotting was performed to determine the levels of RNAIII, the agr regulatory molecule, in two isogenic pairs of...
Projan, S J, Brown-Skrobot, S, Schlievert, P M, Vandenesch, F, Novick, R P
Glycerol monolaurate (GML) is a naturally occurring surfactant that is used widely as an emulsifier in the food and cosmetics industries and is generally regarded as lacking in important biological...
The Staphylococcus aureus plasmid pC221, a 4.6-kilobase multicopy chloramphenicol resistance plasmid that forms plasmid-protein relaxation complexes, was mobilized for transfer by the conjugative...
We present data which indicate that (i) the origin of replication of plasmids pT181 and pC221 can also function as termination signals; (ii) termination of replication occurs when a round of...
Translational fusion with a secretory enzyme as an indicator.
Wang, P Z, Projan, S J, Leason, K R, Novick, R P
A novel type of translational fusion system has been developed by using a secretory protein, staphylococcal beta-lactamase, as an indicator. The beta-lactamase structural gene was modified to provide...
Projan, S J, Monod, M, Narayanan, C S, Dubnau, D
A naturally occurring plasmid from Bacillus subtilis, pIM13, codes for constitutively expressed macrolide-lincosamide-streptogramin B (MLS) resistance, is stably maintained at a high copy number, and...
Isolation and analysis of multicopy extragenic suppressors of dnaA mutations.
Recombinant plasmids were constructed from restriction enzyme digests of Escherichia coli chromosomal deoxyribonucleic acid and pMB9 plasmid deoxyribonucleic acid and selected for correction of the...
Nucleotide sequence: the beta-hemolysin gene of Staphylococcus aureus.
Projan, S J, Kornblum, J, Kreiswirth, B, Moghazeh, S L, Eisner, W, Novick, R P
Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus.
Projan, S J, Moghazeh, S, Novick, R P
pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. The plasmid has a copy number of about 25 per cell, and belongs to the inc5 incompatibility group. The...
A temperature-dependent pBR322 copy number mutant resulting from a Tn5 position effect.
Lupski, J R, Projan, S J, Ozaki, L S, Godson, G N
In the process of randomly mutagenizing a recombinant pBR322 clone with transposon Tn5, a high copy number plasmid mutant, pLO88, has been isolated. The copy number phenotype of pLO88 is observed...
Wang, P Z, Projan, S J, Henriquez, V, Novick, R P
The leading strand replication origin of pT181 plasmids consists of two adjacent inverted repeat elements (IR-II and IR-III), which are involved in origin recognition by the initiator (Rep) protein....
Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule.
Novick, R P, Ross, H F, Projan, S J, Kornblum, J, Kreiswirth, B, Moghazeh, S
The production of most toxins and other exoproteins in Staphylococcus aureus is controlled globally by a complex polycistronic regulatory locus, agr. Secretory proteins are up-regulated by agr...
Uses of Staphylococcus aureus GeneChips in Genotyping and Genetic Composition Analysis
Dunman, P. M., Mounts, W., McAleese, F., Immermann, F., Macapagal, D., Marsilio, E., ...
Understanding the relatedness of strains within a bacterial species is essential for monitoring reservoirs of antimicrobial resistance and for epidemiological studies. Pulsed-field gel...
Novick, R P, Adler, G K, Projan, S J, Carleton, S, Highlander, S K, Gruss, A, ...
pT181 is a fully sequenced 4.4-kb 20 copy Tcr plasmid from Staphylococcus aureus. Its replication system involves a unique unidirectional origin embedded in the coding sequence for a...
Control of pT181 replication II. Mutational analysis.
Carleton, S, Projan, S J, Highlander, S K, Moghazeh, S M, Novick, R P
We describe the isolation and analysis of mutations affecting the regulation of Staphylococcus aureus plasmid pT181 replication. Previous results suggested that regulation is achieved by control of...
MgrA Is a Multiple Regulator of Two New Efflux Pumps in Staphylococcus aureus†
Truong-Bolduc, Q. C., Dunman, P. M., Strahilevitz, J., Projan, S. J., Hooper, D. C.
In an analysis of the resistance mechanisms of an mgrA mutant, we identified two genes encoding previously undescribed transporters, NorB and Tet38. norB was 1,392 bp and encoded a predicted 49-kDa...