Scientific Commons: S. Jaken

Immunocytochemical localization of phospholipase C-gamma in rat embryo fibroblasts.

Rat embryo fibroblasts (REF52) exhibit a distinctive, transformation-sensitive distribution of alpha-protein kinase C (alpha-PKC). Receptor-mediated activation of phospholipase C (PLC)-gamma...

Purification and characterization of three types of protein kinase C from rabbit brain cytosol.

Jaken, S, Kiley, S C

Three types of protein kinase C were purified from rabbit brain cytosol. Each type has a molecular mass of approximately 80 kDa and serves as a receptor for phorbol esters. Polyclonal antibodies...

Overexpression of the alpha-type protein kinase (PK) C in LLC-PK1 cells does not lead to a proportional increase in the induction of two 12-O-tetradecanoylphorbol-13-acetate-inducible genes.

Wartmann, M, Jans, D A, Parker, P J, Nagamine, Y, Hemmings, B A, Jaken, S, ...

Phorbol esters, by activating protein kinase C (PKC), induce the expression of the urokinase-type plasminogen activator (uPA) gene and the proto-oncogene c-fos in LLC-PK1 (PK1) porcine kidney...

Differences in intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells.

Jaken, S, Black, P H

The intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells was investigated by using sucrose density gradient centrifugation. In confluent cells, which...

Differences in the isoelectric focusing patterns of gamma-glutamyl transpeptidase from normal and cancerous rat mammary tissue.

Jaken, S, Mason, M

Highly purified fractions of gamma-glutamyl transpeptidase [gamma-glutamyltrinsferase; (5-glutamyl)-peptide:amino-acid 5-glutamyltransferase, EC 2.3.2.2] from normal and malignant rat mammary tissue...

Selective loss of substrate recognition induced by the tumour-associated D294G point mutation in protein kinase Calpha.

Prévostel, C, Alvaro, V, Vallentin, A, Martin, A, Jaken, S, Joubert, D

The tumour-associated D294G mutant of protein kinase Calpha (PKCalpha) was recently shown not to be translocated to the plasma membrane on stimulation with PMA, in contrast with the wild-type enzyme....