Otabe, E. S., Kiuchi, M., Kawai, S., Morita, Y., Ge, J., Ni, B., ...
Critical current density of polycrystalline bulk SmFeAsO$_{1-x}$F$_x$ specimens prepared by powder in tube (PIT) method and conventional solid state reaction was investigated by remanent magnetic...
Unbound excited states in 19,17C (2007)
Satou, Y., Nakamura, T., Fukuda, N., Sugimoto, T., Kondo, Y., Matsui, N., ...
The neutron-rich carbon isotopes 19,17C have been investigated via proton inelastic scattering on a liquid hydrogen target at 70 MeV/nucleon. The invariant mass method in inverse kinematics was...
Kashem, M. A., Singh, B. R., Kondo, T., Imamul Huq, S. M., Kawai, S.
Various extraction procedures were employed for measuring extractable concentrations of potential toxic elements in soil. The extractability of Cd, Cu, Pb and Zn in four contaminated and four...
M. A. Kashem, B. R. Singh, T. Kondo, S. Kawai
Various extraction procedures were employed for measuring extractable concentrations of potential toxic elements in soil. The extractability of Cd, Cu, Pb and Zn in four contaminated and four...
Objective. To prospectively evaluate the safety of tacrolimus in active rheumatoid arthritis (RA) in elderly patients with insufficient response to disease-modifying antirheumatic drugs (DMARDs)....
Rew, S.B., Peggs, K., Sanjuan, I., Pizzey, A.R., Koishihara, Y., Kawai, S., ...
Purpose: The purpose of this work was to test the suitability of the HM1.24 antigen as a CTL target for immunotherapy of patients with multiple myeloma. Experimental design: Antigen-specific T cells...
Excited states in neutron rich boron isotopes (2005)
Kanungo, R., Elekes, Z., Baba, H., Dombrádi, Zs., Fülöp, Zs., Gibelin, J., ...
New experimental results on in-beam gamma spectroscopy of neutron rich boron isotopes are presented for 17B and its neutron removal fragments 14,15B, after scattering with a H2 target. A gamma...
Inelastic proton scattering on $^{16}$C (2005)
Imai, N., Aoi, N., Sakurai, H., Dombrádi, Zs., Saito, A., ...
We have studied the 2+1 state in neutron-rich 16Cvia inelastic proton scattering in inverse kinematics, using a 33 MeV/nucleon beam. From the angle-integrated cross-section, the deformation parameter...
Search for an isomeric state in $^{19}$C (2005)
Kanungo, R., Elekes, Z., Baba, H., Dombrádi, Zs., Fülö, Zs., Gibelin, J., ...
A search for an isomeric state in the neutron-rich nucleus $^{19}$C is reported. The existence of an isomer in the nucleus is predicted by shell model calculations which also predicts the...
Low-lying excited states in $^{17,19}$C (2005)
Elekes, Z., Dombrádi, Zs., Kanungo, R., Baba, H., Fülöp, Zs., Gibelin, J., ...
$^{17,19}$C nuclei have been investigated by proton inelastic scattering on a liquid hydrogen target at intermediate energies. Two peaks were observed in the Doppler-corrected $\gamma$-ray spectra...
Excited states in neutron rich boron isotopes (2005)
Kanungo, R., Elekes, Z., Baba, H., Dombrádi, Zs., Fülöp, Zs., Gibelin, J., ...
New experimental results on in-beam gamma spectroscopy of neutron rich boron isotopes are presented for 17B and its neutron removal fragments 14,15B, after scattering with a H2 target. A gamma...
Inelastic proton scattering on $^{16}$C (2005)
Imai, N., Aoi, N., Sakurai, H., Dombrádi, Zs., Saito, A., ...
We have studied the 2+1 state in neutron-rich 16Cvia inelastic proton scattering in inverse kinematics, using a 33 MeV/nucleon beam. From the angle-integrated cross-section, the deformation parameter...
Search for an isomeric state in $^{19}$C (2005)
Kanungo, R., Elekes, Z., Baba, H., Dombrádi, Zs., Fülö, Zs., Gibelin, J., ...
A search for an isomeric state in the neutron-rich nucleus $^{19}$C is reported. The existence of an isomer in the nucleus is predicted by shell model calculations which also predicts the...
Low-lying excited states in $^{17,19}$C (2005)
Elekes, Z., Dombrádi, Zs., Kanungo, R., Baba, H., Fülöp, Zs., Gibelin, J., ...
$^{17,19}$C nuclei have been investigated by proton inelastic scattering on a liquid hydrogen target at intermediate energies. Two peaks were observed in the Doppler-corrected $\gamma$-ray spectra...
Diagnostics of microdischarge-integrated plasma sources for display and materials processing (2005)
Tachibana, K, Kishimoto, Y, Kawai, S, Sakaguchi, T, Sakai, O
Objective. To prospectively evaluate the safety of tacrolimus in active rheumatoid arthritis (RA) in elderly patients with insufficient response to disease-modifying antirheumatic drugs (DMARDs)....
Bound excited states in$^{27}$F (2004)
Elekes, Z., Dombrádi, Zs., Saito, A., Aoi, N., Baba, H., Demichi, K., ...
The 1H(27F, 25,26,27F) reactions have been studied at 40 MeV/nucleon average energy using a liquid hydrogen target. For 25F, 26F and 27F nuclei, we have observed two γ-ray peaks each originating...
Bound excited states in$^{27}$F (2004)
Elekes, Z., Dombrádi, Zs., Saito, A., Aoi, N., Baba, H., Demichi, K., ...
The 1H(27F, 25,26,27F) reactions have been studied at 40 MeV/nucleon average energy using a liquid hydrogen target. For 25F, 26F and 27F nuclei, we have observed two γ-ray peaks each originating...
Kim, GM, Kawai, S, Nagashio, M, Kawakatsu, H, Brugger, J
We report an all-dry, two-step, surface nanoengineering method to fabricate nanomechanical elements without photolithography. It is based on the local deposition through a nanostencil of a...
Kawai, S, Kusunoki, N, Yamazaki, R
No abstract available.
Pathogenic roles of IL-6 and IL-6 blocking therapy on rheumatoid arthritis (2003)
Yoshizaki, K, Nishimoto, N, Miyasaka, N, Yamamoto, K, Kawai, S, Takeuchi, T, ...
No abstract available.
Kusunoki, N, Yamazaki, R, Kawai, S
No abstract available.
Optically addressing a large number of cantilevers (2003)
Kobayashi, D, Kawai, S, Nagashio, M, Saya, D, Toshiyoshi, H, Fujita, H, ...
A heterodyne laser Doppler interferometer operating up to 100 MHz with an optical vibration excitation function was implemented to enable frequency detection of nanocantilevers. By using the...
90 MHz nanomechanical structures fabricated by stencil deposition and dry etching (2003)
Kim, G M, Kawai, S, Kawakatsu, H, Brugger, J
We present a simple method for fabricating nanomechanical elements with ultra high resonance frequency. The process is based on local deposition through a miniaturized shadow mask (nanostencil),...
90 MHz nanomechanical structures fabricated by stencil deposition and dry etching (2003)
Kim, G M, Kawai, S, Kawakatsu, H, Brugger, J
We present a simple method for fabricating fast resonating (90 MHz) nanomechanical elements based on the local deposition through a miniaturized shadow mask (nanostencil), followed by dry etching to...
Moulded Products from Radiata Pine Bark (2001)
MASUDA, K., YANO, Hiroyuki, KAWAI, S.
この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。
Tanaka, H., Nagai, E., Murata, H., Tsubone, T., Shirakura, Y., Sugiyama, T., ...
Objective. To investigate the function of bone morphogenic protein‐2 (BMP‐2) in the ossification of the spinal ligament (OSL). Methods. Total RNA was prepared...
ELECTRON SPIN RESONANCE IN THE DISORDERED SYSTEM Mg1-xCoxCl2 (1988)
Katsumata, K., Kawai, S., Tuchendler, J.
Electron spin resonance experiments performed on a single crystal sample of the site diluted planar magnet Mg0.79Co0.21Cl2 are reported. A number of resonance lines has been observed. From the...
ELECTRON SPIN RESONANCE IN THE DISORDERED SYSTEM Mg1-xCoxCl2 (1988)
Katsumata, K., Kawai, S., Tuchendler, J.
Electron spin resonance experiments performed on a single crystal sample of the site diluted planar magnet Mg0.79Co0.21Cl2 are reported. A number of resonance lines has been observed. From the...
ELECTRON SPIN RESONANCE IN THE DISORDERED SYSTEM Mg1-xCoxCl2 (1988)
Katsumata, K., Kawai, S., Tuchendler, J.
Electron spin resonance experiments performed on a single crystal sample of the site diluted planar magnet Mg0.79Co0.21Cl2 are reported. A number of resonance lines has been observed. From the...
New polymeric model substrates for the study of microbial ligninolysis.
Kawai, S, Jensen, K A, Bao, W, Hammel, K E
Lignin model dimers are valuable tools for the elucidation of microbial ligninolytic mechanisms, but their low molecular weight (MW) makes them susceptible to nonligninolytic intracellular...
Kawai, S, Suzuki, H, Yamamoto, K, Inui, M, Yukawa, H, Kumagai, H
Malic enzyme (EC 1.1.1.39), which catalyzes L-malate oxidative decarboxylation and pyruvate reductive carboxylation, was purified to homogeneity from Streptococcus bovis ATCC 15352, and properties of...
Comparison of the nucleic acids of helical and coccoid forms of Helicobacter pylori.
Narikawa, S, Kawai, S, Aoshima, H, Kawamata, O, Kawaguchi, R, Hikiji, K, ...
The nucleic acids of the helical and coccoid forms of Helicobacter pylori were studied to determine if the coccoid forms are "viable (capable of growing) but nonculturable." Using a reference strain...
Genome structure of avian sarcoma virus Y73 and unique sequence coding for polyprotein p90.
Yoshida, M, Kawai, S, Toyoshima, K
The genome structure of a newly isolated sarcoma virus, Y73, was studied. Y73 is a defective, potent sarcomagenic virus and contains 4.8-kilobase (kb) RNA as its genome; in contrast, helper virus...
Characterization of the L-malate permease gene (maeP) of Streptococcus bovis ATCC 15352.
Kawai, S, Suzuki, H, Yamamoto, K, Kumagai, H
A gene which was shown to be cotranscribed with the NAD+-dependent malic enzyme gene (maeE) of Streptococcus bovis ATCC 15352 was revealed to encode L-malate-specific permease (MaeP), which showed...
Kawai, S, Murao, S, Mochizuki, M, Shibuya, I, Yano, K, Takagi, M
Cycloheximide is one of the antibiotics that inhibit protein synthesis in most eukaryotic cells. We have found that a yeast, Candida maltosa, is resistant to the drug because it possesses a...
Masai, E, Katayama, Y, Kawai, S, Nishikawa, S, Yamasaki, M, Morohoshi, N
We isolated Pseudomonas paucimobilis SYK-6, which was able to degrade various dimeric lignin compounds (Y. Katayama, S. Nishikawa, M. Nakamura, K. Yano, M. Yamasaki, N. Morohoshi, and T. Haraguchi,...
Takagi, M, Kawai, S, Chang, M C, Shibuya, I, Yano, K
To construct a host-vector system in an n-alkane-assimilating yeast, Candida maltosa, the isolation of an ARS site from its genome which replicates autonomously in C. maltosa was attempted. Leu-...
Takagi, M, Kawai, S, Shibuya, I, Miyazaki, M, Yano, K
We have previously shown that cycloheximide resistance can be induced in a strain of Candida maltosa by modifying ribosomes (M. Takagi, S. Kawai, Y. Takata, N. Tanaka, M. Sunairi, M. Miyazaki, and K....
Kimura, M, Miyake, H, Kim, H S, Tanabe, M, Arai, M, Kawai, S, ...
A simple and convenient PCR method that amplifies the 18S rRNA genes has been developed for the purpose of detecting and differentiating four species causing malaria in humans. The advantage of the...
Kawamoto, F, Miyake, H, Kaneko, O, Kimura, M, Nguyen, T D, Nguyen, T D, ...
Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate...
Structure-function analysis of the maf oncogene product, a member of the b-Zip protein family.
Kataoka, K, Nishizawa, M, Kawai, S
The v-maf oncogene, identified as the transforming gene of the avian retrovirus AS42, encodes a protein containing a b-Zip motif. From this structural feature, the v-Maf protein was expected to form...
Shiroki, K, Hamaguchi, M, Kawai, S
When rat 3Y1 cells were infected with Rous sarcoma virus (RSV) variant SR-RSV-D(H), many 3Y1 cells acquired a stable provirus but only few of them formed transformed foci. In contrast, 12E1AY cells...
The expression of the human beta interferon (IFN-beta) gene was activated by the adenovirus E1B-19K protein. The sequence within the IFN-beta promoter which is related to the activation was analyzed...
Infrequent involvement of c-fos in avian leukosis virus-induced nephroblastoma.
Collart, K L, Aurigemma, R, Smith, R E, Kawai, S, Robinson, H L
To determine whether c-fos is involved in avian leukosis virus-induced nephroblastoma, 28 tumors from chickens were analyzed for novel fos fragments. DNA from 1 of 16 clonal outgrowths (in chicken...
Nishizawa, M, Mayer, B J, Takeya, T, Yamamoto, T, Toyoshima, K, Hanafusa, H, ...
We molecularly cloned the src coding region of tsNY68, a mutant of Rous sarcoma virus temperature sensitive (ts) for transformation, and constructed a series of ts wild-type recombinant src genes....
Cell transformation by a virus containing a molecularly constructed gag-erbB fused gene.
Kawai, S, Nishizawa, M, Yamamoto, T, Toyoshima, K
A provirus DNA that contains a gag-erbB fused gene as the sole and transforming gene was molecularly constructed from plasmid pSRA2 containing the entire genome of Rous sarcoma virus and pAE7.7...
A mutant derived from a temperature-sensitive mutant of Rous sarcoma virus ( tsNY68 ) which showed extremely low infectivity was characterized. Infection of chicken embryo fibroblast cells with the...
Koyama, T, Harada, F, Kawai, S
The accompanying paper (S. Kawai and T. Koyama , J. Virol. 51:147-153, 1984) describes the isolation and biological properties of a mutant, TK15 , derived from a Rous sarcoma virus mutant, tsNY68 ....
Unusual features of the leader sequence of Rous sarcoma virus packaging mutant TK15.
Nishizawa, M, Koyama, T, Kawai, S
TK15, a mutant derived from a temperature-sensitive mutant of Rous sarcoma virus (tsNY68), has extremely low infectivity although it has intact viral genes. Biological and biochemical analyses of the...
Frequent segregation of more-defective variants from a Rous sarcoma virus packaging mutant, TK15.
Nishizawa, M, Koyama, T, Kawai, S
TK15, a mutant derived from a temperature-sensitive mutant of Rous sarcoma virus (tsNY68), has extremely low infectivity although it has intact viral genes. Previous analyses of the virus and...
Nishizawa, M, Goto, N, Kawai, S
A new avian transforming retrovirus, NK24, was isolated from a chicken with a nephroblastoma. This transforming virus induced fibrosarcomas with osteogenic cell proliferation and nephroblastomas in...
The chicken c-fos gene: cloning and nucleotide sequence analysis.
Fujiwara, K T, Ashida, K, Nishina, H, Iba, H, Miyajima, N, Nishizawa, M, ...
Using a DNA probe from an avian transforming virus, NK24, that contains the fos gene and that was newly isolated and characterized (M. Nishizawa, N. Goto, and S. Kawai, J. Virol. 61:3733-3740, 1987),...
Koide, H, Yokoyama, S, Kawai, G, Ha, J M, Oka, T, Kawai, S, ...
Endeavoring to develop a method to biosynthesize proteins substituted with nonprotein amino acids, we attempted the incorporation of L-2-aminohexanoic acid (Ahx) into human epidermal growth factor...
Antibody to phosphatidylinositol 4,5-bisphosphate inhibits oncogene-induced mitogenesis.
Fukami, K, Matsuoka, K, Nakanishi, O, Yamakawa, A, Kawai, S, Takenawa, T
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to be enhanced in cells transformed by some types of oncogenes and tumor viruses, but it is still unknown whether the...
Transformation of rat cells by fusion-infection with Rous sarcoma virus.
The transformation of a rat cell line, 3Y1, by nonmammalian tropic strains of avian sarcoma virus was tested using cell-virus fusion mediated by Sendai virus or polyethylene glycol. Furthermore, the...
v-maf, a viral oncogene that encodes a "leucine zipper" motif.
Nishizawa, M, Kataoka, K, Goto, N, Fujiwara, K T, Kawai, S
We have molecularly cloned the provirus of the avian musculoaponeurotic fibrosarcoma virus AS42. Nucleotide sequence analysis of a biologically active clone of AS42 showed that this virus encodes a...
Wang, L H, Duesberg, P H, Kawai, S, Hanafusa, H
Envelope-specific and sarcoma-specific nucleotide sequences have been located within the 10,000 nucleotides of the RNA of nondefective Schmidt-Ruppin Rous sarcoma virus (nd SR). For this purpose,...
Kawai, S, Yoshida, M, Segawa, K, Sugiyama, H, Ishizaki, R, Toyoshima, K
The Y73 strain of avian sarcoma virus recently isolated in Japan is defective in replication and is associated with subgroup A leukosis virus (YAV). The virus caused sarcoma but not acute leukosis...
Tal, J, Fujita, D J, Kawai, S, Varmus, H E, Bishop, J M
The env gene of avian leukosis-sarcoma viruses encodes a glycoprotein that determines the host range and surface antigenicitiy of virions. We have purified radioactive DNA (cDNAgp) complementary to...
Recombination between a temperature-sensitive mutant and a deletion mutant of Rous sarcoma virus.
Cells doubly infected with two mutants of the Schmidt-Ruppin strain of Rous sarcoma virus (RSV), ts68, which is temperature sensitive for cell transformation (srcts), and a deletion mutant, N8, which...
Semba, K, Kawai, S, Matsuzawa, Y, Yamanashi, Y, Nishizawa, M, Toyoshima, K
The transforming activity of the human fyn protein, p59fyn, which is a kinase of the src family, was investigated by testing the effect of recombinant avian retrovirus (Fyn virus) expressing p59fyn...
Molecular cloning and characterization of an activated human c-raf-1 gene.
Fukui, M, Yamamoto, T, Kawai, S, Mitsunobu, F, Toyoshima, K
Results of previous studies have shown that a raf-related transforming DNA sequence is present in NIH 3T3 transformants that are derived from GL-5-JCK human glioblastoma DNA transfection. The...
Activation of the cellular src gene by transducing retrovirus.
Ikawa, S, Hagino-Yamagishi, K, Kawai, S, Yamamoto, T, Toyoshima, K
Newly isolated strains of avian sarcoma virus, S1 and S2, were shown to have the transduced cellular src gene as their viral transforming gene (Yamagishi et al., Virology 137:266-275, 1984). In this...
New procedure for DNA transfection with polycation and dimethyl sulfoxide.
A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to...
Fukui, M, Yamamoto, T, Kawai, S, Maruo, K, Toyoshima, K
High molecular weight DNAs prepared from a variety of human tumors maintained in nude mice were assayed for their ability to transform NIH 3T3 cells. DNAs from 4 of 21 tumors tested induced...
RNA of replication-defective strains of Rous sarcoma virus.
Duesberg, P H, Kawai, S, Wang, L H, Vogt, P K, Murphy, H M, Hanafusa, H
The RNA of a replication-defective (rd) mutant, isolated from stocks of nondefective (nd) Schmidt-Ruppin Rous sarcoma virus of subgroup A (SR-A) and termed SR-N8, was compared to the RNAs of SR-A, of...
Relationship between A-type and C-type particles in cells infected by Rous sarcoma virus.
De Giuli, C, Hanafusa, H, Kawai, S, Dales, S, Chen, J H, Hsu, K C
Chicken cells infected with avian RNA tumor virus often contain small cytoplasmic A-type particles which commonly exist as clusters of 50--100 particles when viewed in thin sections. These particles...
Normal distribution of collagen IV in renal basement membranes in Epstein's syndrome.
Naito, I, Nomura, S, Inoue, S, Kagawa, M, Kawai, S, Gunshin, Y, ...
BACKGROUND: Epstein's syndrome is defined as a subtype of Alport's syndrome, and is distinguished from the other subtypes by accompanying macrothrombocytopenia. Mutations in collagen IV genes are...
Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying length.
Kawai, S, Duesberg, P H, Hanafusa, H
The RNAs of transformation-defective (td) deletion mutants of the Schmidt-Ruppin strain of Rous sarcoma virus were found to vary in size when compared by polyacrylamide gel electrophoresis. Three of...
Jensen, K. A., Bao, W., Kawai, S., Srebotnik, E., Hammel, K. E.
Many ligninolytic fungi appear to lack lignin peroxidase (LiP), the enzyme generally thought to cleave the major, recalcitrant, nonphenolic structures in lignin. At least one such fungus,...
Srebotnik, E., Jensen, K. A., Kawai, S., Hammel, K. E.
The white-rot fungus Ceriporiopsis subvermispora is able to degrade nonphenolic lignin structures but appears to lack lignin peroxidase (LiP), which is generally thought to be responsible for these...
Jensen, K. A., Bao, W., Kawai, S., Srebotnik, E., Hammel, K. E.
Vol. 62, no. 10, p. 3684, column 2, line 16: "Tri(methylsilyl)" should read "Tri(trimethylsilyl)." Line 17: "Di(methylsilyl)" should read "Di(trimethylsilyl)." Line 20: "Tri(methylsilyl)" should read...
New polymeric model substrates for the study of microbial ligninolysis.
Kawai, S, Jensen, K A, Bao, W, Hammel, K E
Lignin model dimers are valuable tools for the elucidation of microbial ligninolytic mechanisms, but their low molecular weight (MW) makes them susceptible to nonligninolytic intracellular...
Kawai, S, Suzuki, H, Yamamoto, K, Inui, M, Yukawa, H, Kumagai, H
Malic enzyme (EC 1.1.1.39), which catalyzes L-malate oxidative decarboxylation and pyruvate reductive carboxylation, was purified to homogeneity from Streptococcus bovis ATCC 15352, and properties of...
Comparison of the nucleic acids of helical and coccoid forms of Helicobacter pylori.
Narikawa, S, Kawai, S, Aoshima, H, Kawamata, O, Kawaguchi, R, Hikiji, K, ...
The nucleic acids of the helical and coccoid forms of Helicobacter pylori were studied to determine if the coccoid forms are "viable (capable of growing) but nonculturable." Using a reference strain...
Genome structure of avian sarcoma virus Y73 and unique sequence coding for polyprotein p90.
Yoshida, M, Kawai, S, Toyoshima, K
The genome structure of a newly isolated sarcoma virus, Y73, was studied. Y73 is a defective, potent sarcomagenic virus and contains 4.8-kilobase (kb) RNA as its genome; in contrast, helper virus...
Characterization of the L-malate permease gene (maeP) of Streptococcus bovis ATCC 15352.
Kawai, S, Suzuki, H, Yamamoto, K, Kumagai, H
A gene which was shown to be cotranscribed with the NAD+-dependent malic enzyme gene (maeE) of Streptococcus bovis ATCC 15352 was revealed to encode L-malate-specific permease (MaeP), which showed...
Kawai, S, Murao, S, Mochizuki, M, Shibuya, I, Yano, K, Takagi, M
Cycloheximide is one of the antibiotics that inhibit protein synthesis in most eukaryotic cells. We have found that a yeast, Candida maltosa, is resistant to the drug because it possesses a...
Masai, E, Katayama, Y, Kawai, S, Nishikawa, S, Yamasaki, M, Morohoshi, N
We isolated Pseudomonas paucimobilis SYK-6, which was able to degrade various dimeric lignin compounds (Y. Katayama, S. Nishikawa, M. Nakamura, K. Yano, M. Yamasaki, N. Morohoshi, and T. Haraguchi,...
Takagi, M, Kawai, S, Chang, M C, Shibuya, I, Yano, K
To construct a host-vector system in an n-alkane-assimilating yeast, Candida maltosa, the isolation of an ARS site from its genome which replicates autonomously in C. maltosa was attempted. Leu-...
Takagi, M, Kawai, S, Shibuya, I, Miyazaki, M, Yano, K
We have previously shown that cycloheximide resistance can be induced in a strain of Candida maltosa by modifying ribosomes (M. Takagi, S. Kawai, Y. Takata, N. Tanaka, M. Sunairi, M. Miyazaki, and K....
Kimura, M, Miyake, H, Kim, H S, Tanabe, M, Arai, M, Kawai, S, ...
A simple and convenient PCR method that amplifies the 18S rRNA genes has been developed for the purpose of detecting and differentiating four species causing malaria in humans. The advantage of the...
Kawamoto, F, Miyake, H, Kaneko, O, Kimura, M, Nguyen, T D, Nguyen, T D, ...
Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate...
Structure-function analysis of the maf oncogene product, a member of the b-Zip protein family.
Kataoka, K, Nishizawa, M, Kawai, S
The v-maf oncogene, identified as the transforming gene of the avian retrovirus AS42, encodes a protein containing a b-Zip motif. From this structural feature, the v-Maf protein was expected to form...
Shiroki, K, Hamaguchi, M, Kawai, S
When rat 3Y1 cells were infected with Rous sarcoma virus (RSV) variant SR-RSV-D(H), many 3Y1 cells acquired a stable provirus but only few of them formed transformed foci. In contrast, 12E1AY cells...
The expression of the human beta interferon (IFN-beta) gene was activated by the adenovirus E1B-19K protein. The sequence within the IFN-beta promoter which is related to the activation was analyzed...
Infrequent involvement of c-fos in avian leukosis virus-induced nephroblastoma.
Collart, K L, Aurigemma, R, Smith, R E, Kawai, S, Robinson, H L
To determine whether c-fos is involved in avian leukosis virus-induced nephroblastoma, 28 tumors from chickens were analyzed for novel fos fragments. DNA from 1 of 16 clonal outgrowths (in chicken...
Nishizawa, M, Mayer, B J, Takeya, T, Yamamoto, T, Toyoshima, K, Hanafusa, H, ...
We molecularly cloned the src coding region of tsNY68, a mutant of Rous sarcoma virus temperature sensitive (ts) for transformation, and constructed a series of ts wild-type recombinant src genes....
Cell transformation by a virus containing a molecularly constructed gag-erbB fused gene.
Kawai, S, Nishizawa, M, Yamamoto, T, Toyoshima, K
A provirus DNA that contains a gag-erbB fused gene as the sole and transforming gene was molecularly constructed from plasmid pSRA2 containing the entire genome of Rous sarcoma virus and pAE7.7...
A mutant derived from a temperature-sensitive mutant of Rous sarcoma virus ( tsNY68 ) which showed extremely low infectivity was characterized. Infection of chicken embryo fibroblast cells with the...
Koyama, T, Harada, F, Kawai, S
The accompanying paper (S. Kawai and T. Koyama , J. Virol. 51:147-153, 1984) describes the isolation and biological properties of a mutant, TK15 , derived from a Rous sarcoma virus mutant, tsNY68 ....
Unusual features of the leader sequence of Rous sarcoma virus packaging mutant TK15.
Nishizawa, M, Koyama, T, Kawai, S
TK15, a mutant derived from a temperature-sensitive mutant of Rous sarcoma virus (tsNY68), has extremely low infectivity although it has intact viral genes. Biological and biochemical analyses of the...
Frequent segregation of more-defective variants from a Rous sarcoma virus packaging mutant, TK15.
Nishizawa, M, Koyama, T, Kawai, S
TK15, a mutant derived from a temperature-sensitive mutant of Rous sarcoma virus (tsNY68), has extremely low infectivity although it has intact viral genes. Previous analyses of the virus and...
Nishizawa, M, Goto, N, Kawai, S
A new avian transforming retrovirus, NK24, was isolated from a chicken with a nephroblastoma. This transforming virus induced fibrosarcomas with osteogenic cell proliferation and nephroblastomas in...
The chicken c-fos gene: cloning and nucleotide sequence analysis.
Fujiwara, K T, Ashida, K, Nishina, H, Iba, H, Miyajima, N, Nishizawa, M, ...
Using a DNA probe from an avian transforming virus, NK24, that contains the fos gene and that was newly isolated and characterized (M. Nishizawa, N. Goto, and S. Kawai, J. Virol. 61:3733-3740, 1987),...
Koide, H, Yokoyama, S, Kawai, G, Ha, J M, Oka, T, Kawai, S, ...
Endeavoring to develop a method to biosynthesize proteins substituted with nonprotein amino acids, we attempted the incorporation of L-2-aminohexanoic acid (Ahx) into human epidermal growth factor...
Antibody to phosphatidylinositol 4,5-bisphosphate inhibits oncogene-induced mitogenesis.
Fukami, K, Matsuoka, K, Nakanishi, O, Yamakawa, A, Kawai, S, Takenawa, T
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to be enhanced in cells transformed by some types of oncogenes and tumor viruses, but it is still unknown whether the...
Transformation of rat cells by fusion-infection with Rous sarcoma virus.
The transformation of a rat cell line, 3Y1, by nonmammalian tropic strains of avian sarcoma virus was tested using cell-virus fusion mediated by Sendai virus or polyethylene glycol. Furthermore, the...
v-maf, a viral oncogene that encodes a "leucine zipper" motif.
Nishizawa, M, Kataoka, K, Goto, N, Fujiwara, K T, Kawai, S
We have molecularly cloned the provirus of the avian musculoaponeurotic fibrosarcoma virus AS42. Nucleotide sequence analysis of a biologically active clone of AS42 showed that this virus encodes a...
Wang, L H, Duesberg, P H, Kawai, S, Hanafusa, H
Envelope-specific and sarcoma-specific nucleotide sequences have been located within the 10,000 nucleotides of the RNA of nondefective Schmidt-Ruppin Rous sarcoma virus (nd SR). For this purpose,...
Kawai, S, Yoshida, M, Segawa, K, Sugiyama, H, Ishizaki, R, Toyoshima, K
The Y73 strain of avian sarcoma virus recently isolated in Japan is defective in replication and is associated with subgroup A leukosis virus (YAV). The virus caused sarcoma but not acute leukosis...
Tal, J, Fujita, D J, Kawai, S, Varmus, H E, Bishop, J M
The env gene of avian leukosis-sarcoma viruses encodes a glycoprotein that determines the host range and surface antigenicitiy of virions. We have purified radioactive DNA (cDNAgp) complementary to...
Recombination between a temperature-sensitive mutant and a deletion mutant of Rous sarcoma virus.
Cells doubly infected with two mutants of the Schmidt-Ruppin strain of Rous sarcoma virus (RSV), ts68, which is temperature sensitive for cell transformation (srcts), and a deletion mutant, N8, which...
Semba, K, Kawai, S, Matsuzawa, Y, Yamanashi, Y, Nishizawa, M, Toyoshima, K
The transforming activity of the human fyn protein, p59fyn, which is a kinase of the src family, was investigated by testing the effect of recombinant avian retrovirus (Fyn virus) expressing p59fyn...
Molecular cloning and characterization of an activated human c-raf-1 gene.
Fukui, M, Yamamoto, T, Kawai, S, Mitsunobu, F, Toyoshima, K
Results of previous studies have shown that a raf-related transforming DNA sequence is present in NIH 3T3 transformants that are derived from GL-5-JCK human glioblastoma DNA transfection. The...
Activation of the cellular src gene by transducing retrovirus.
Ikawa, S, Hagino-Yamagishi, K, Kawai, S, Yamamoto, T, Toyoshima, K
Newly isolated strains of avian sarcoma virus, S1 and S2, were shown to have the transduced cellular src gene as their viral transforming gene (Yamagishi et al., Virology 137:266-275, 1984). In this...
New procedure for DNA transfection with polycation and dimethyl sulfoxide.
A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to...
Fukui, M, Yamamoto, T, Kawai, S, Maruo, K, Toyoshima, K
High molecular weight DNAs prepared from a variety of human tumors maintained in nude mice were assayed for their ability to transform NIH 3T3 cells. DNAs from 4 of 21 tumors tested induced...
RNA of replication-defective strains of Rous sarcoma virus.
Duesberg, P H, Kawai, S, Wang, L H, Vogt, P K, Murphy, H M, Hanafusa, H
The RNA of a replication-defective (rd) mutant, isolated from stocks of nondefective (nd) Schmidt-Ruppin Rous sarcoma virus of subgroup A (SR-A) and termed SR-N8, was compared to the RNAs of SR-A, of...
Relationship between A-type and C-type particles in cells infected by Rous sarcoma virus.
De Giuli, C, Hanafusa, H, Kawai, S, Dales, S, Chen, J H, Hsu, K C
Chicken cells infected with avian RNA tumor virus often contain small cytoplasmic A-type particles which commonly exist as clusters of 50--100 particles when viewed in thin sections. These particles...
Normal distribution of collagen IV in renal basement membranes in Epstein's syndrome.
Naito, I, Nomura, S, Inoue, S, Kagawa, M, Kawai, S, Gunshin, Y, ...
BACKGROUND: Epstein's syndrome is defined as a subtype of Alport's syndrome, and is distinguished from the other subtypes by accompanying macrothrombocytopenia. Mutations in collagen IV genes are...
Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying length.
Kawai, S, Duesberg, P H, Hanafusa, H
The RNAs of transformation-defective (td) deletion mutants of the Schmidt-Ruppin strain of Rous sarcoma virus were found to vary in size when compared by polyacrylamide gel electrophoresis. Three of...
Jensen, K. A., Bao, W., Kawai, S., Srebotnik, E., Hammel, K. E.
Many ligninolytic fungi appear to lack lignin peroxidase (LiP), the enzyme generally thought to cleave the major, recalcitrant, nonphenolic structures in lignin. At least one such fungus,...
Srebotnik, E., Jensen, K. A., Kawai, S., Hammel, K. E.
The white-rot fungus Ceriporiopsis subvermispora is able to degrade nonphenolic lignin structures but appears to lack lignin peroxidase (LiP), which is generally thought to be responsible for these...
Jensen, K. A., Bao, W., Kawai, S., Srebotnik, E., Hammel, K. E.
Vol. 62, no. 10, p. 3684, column 2, line 16: "Tri(methylsilyl)" should read "Tri(trimethylsilyl)." Line 17: "Di(methylsilyl)" should read "Di(trimethylsilyl)." Line 20: "Tri(methylsilyl)" should read...
Giant aneurysm of the left main coronary artery in Takayasu aortitis
Suzuki, H, Daida, H, Tanaka, M, Sato, H, Kawai, S, Sakurai, H, ...
Takayasu aortitis is a chronic inflammatory vasculitis characterised by stenosis or obliteration of large and medium sized arteries. Although coronary arteries are affected in approximately 10% of...
Matsui, T, Akahoshi, T, Namai, R, Hashimoto, A, Kurihara, Y, Rana, M, ...
Infiltration of various types of leucocytes has been shown to play a crucial role in the pathogenesis of rheumatoid arthritis (RA). Macrophage inflammatory protein-3α (MIP-3α) is a recently...
Serum 25-hydroxy-vitamin D in hepatobiliary disease in infancy.
Kobayashi, A, Kawai, S, Ohkubo, M, Ohbe, Y
Serum 25-hydroxy-vitamin D (25-OHD) concentrations were measured in 49 patients with hepatobiliary disease in infancy. Low mean values were found in groups of patients with biliary atresia, neonatal...